Restriction map for
pFN21AB7402
(Circular) (Six-base) MAPSORT of: pFN21AB7402.seq Check: 8680 from: 1 to: 5794
pFN21AB7402 5794 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3604 278
Size: 53 83 186 936 2068 2468
Fragments arranged by size:
2468 2068 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3137 1937
Size: 1200 4594
Acc65I G'GTAC_C
Cuts at: 2470 3115 2470
Size: 645 5149
AclI AA'CG_TT
Cuts at: 1563 3921 4294 1563
Size: 2358 373 3063
Fragments arranged by size:
3063 2358 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3857 5241 5509 1632
Size: 2225 1384 268 1917
Fragments arranged by size:
2225 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2219 820
Size: 197 1202 4395
Fragments arranged by size:
4395 1202 197
AflIII A'CryG_T
Cuts at: 1203 4693 5670 1203
Size: 3490 977 1327
Fragments arranged by size:
3490 1327 977
AgeI A'CCGG_T
Cuts at: 4625 4625
Size: 5794
AhdI GACnn_n'nnGTC
Cuts at: 4523 4523
Size: 5794
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5794
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5794
AlwNI CAG_nnn'CTG
Cuts at: 2524 3537 5109 2524
Size: 1013 1572 3209
Fragments arranged by size:
3209 1572 1013
ApaI G_GGCC'C
Cuts at: 2732 2732
Size: 5794
ApaLI G'TGCA_C
Cuts at: 3851 5007 3851
Size: 1156 4638
ApoI r'AATT_y
Cuts at: 1493 2845 3103 3329 3481 5590 1493
Size: 1352 258 226 152 2109 1697
Fragments arranged by size:
2109 1697 1352 258 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4348 160
Size: 872 3316 1606
Fragments arranged by size:
3316 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5794
AvaI C'yCGr_G
Cuts at: 1870 1943 2027 3119 1870
Size: 73 84 1092 4545
Fragments arranged by size:
4545 1092 84 73
BamHI G'GATC_C
Cuts at: 3124 3491 3124
Size: 367 5427
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2104 2443 2470 2546 3115
Size: 325 217 627 317 339 27 76 569
Cuts at: 3115 4570 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 569 339 325 317 217
76 27
BanII G_rGCy'C
Cuts at: 1391 2732 2872 3113 1391
Size: 1341 140 241 4072
Fragments arranged by size:
4072 1341 241 140
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4823
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2626 2660 3985 4019 1903
Size: 34 50 34 605 34 1325 34 3678
Fragments arranged by size:
3678 1325 605 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2794 3686 4896 2794
Size: 892 1210 3692
Fragments arranged by size:
3692 1210 892
BclI T'GATC_A
Cuts at: 1548 2238 1548
Size: 690 5104
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4405 136
Size: 107 122 71 631 605 2733 1525
Fragments arranged by size:
2733 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5789 5789
Size: 5794
BlpI GC'TnA_GC
Cuts at: 2648 3211 2648
Size: 563 5231
Bme1580I G_kGCm'C
Cuts at: 1790 2715 2732 3855 5011 1790
Size: 925 17 1123 1156 2573
Fragments arranged by size:
2573 1156 1123 925 17
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5794
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3525 4483 442
Size: 416 53 83 399 24 2108 958 1753
Fragments arranged by size:
2108 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5794
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2062 2741 2903 4454 1239
Size: 823 679 162 1551 2579
Fragments arranged by size:
2579 1551 823 679 162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2132 3919 4784 5082 2132
Size: 1787 865 298 2844
Fragments arranged by size:
2844 1787 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2168 4457 882
Size: 617 669 2289 2219
Fragments arranged by size:
2289 2219 669 617
BsaAI yAC'GTr
Cuts at: 493 1204 2124 3585 493
Size: 711 920 1461 2702
Fragments arranged by size:
2702 1461 920 711
BsaBI GATnn'nnATC
Cuts at: 2687 5788 2687
Size: 3101 2693
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3601 3983 4618 275
Size: 53 83 186 936 2068 382 635 1451
Fragments arranged by size:
2068 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2532 4227 4625 4899 5046 5457 2532
Size: 1695 398 274 147 411 2869
Fragments arranged by size:
2869 1695 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2318 2348 2543 2573 2753
Size: 30 165 30 915 30 195 30 180
Cuts at: 2753 2783 1178
Size: 30 4189
Fragments arranged by size:
4189 915 195 180 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2221 2789 1186
Size: 1035 568 4191
Fragments arranged by size:
4191 1035 568
BseYI C'CCAG_C
Cuts at: 4997 5493 4997
Size: 496 5298
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2356 2854 1163
Size: 573 620 498 4103
Fragments arranged by size:
4103 620 573 498
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3244 4005 5033 1540
Size: 275 186 1243 761 1028 2301
Fragments arranged by size:
2301 1243 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2359 3855 3940 5011 2359
Size: 1496 85 1071 3142
Fragments arranged by size:
3142 1496 1071 85
BsmI GAATG_Cn'
Cuts at: 3305 3398 3305
Size: 93 5701
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2359 2715 2732 2872 3113 3855 3940
Size: 399 569 356 17 140 241 742 85
Cuts at: 3940 5011 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 569 399 356 241 140
85 17
BspEI T'CCGG_A
Cuts at: 5457 5457
Size: 5794
BspHI T'CATG_A
Cuts at: 2739 3683 5413 2739
Size: 944 1730 3120
Fragments arranged by size:
3120 1730 944
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2242 3149 844
Size: 1398 907 3489
Fragments arranged by size:
3489 1398 907
BsrBI CCG'CTC
Cuts at: 1394 1823 3681 1394
Size: 429 1858 3507
Fragments arranged by size:
3507 1858 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3072 4289 4463 65
Size: 1183 1824 1217 174 1396
Fragments arranged by size:
1824 1396 1217 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4438 4625 1700
Size: 110 2628 187 2869
Fragments arranged by size:
2869 2628 187 110
BsrGI T'GTAC_A
Cuts at: 96 2274 96
Size: 2178 3616
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5794
BssSI C'ACGA_G
Cuts at: 3854 4866 3854
Size: 1012 4782
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5794
BstBI TT'CG_AA
Cuts at: 4598 4598
Size: 5794
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5794
BstYI r'GATC_y
Cuts at: 1972 2259 2382 3124 3491 3876 3893 5345 5431
Size: 287 123 742 367 385 17 1452 86
Cuts at: 5431 5443 5789 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 742 385 367 346 287 123
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2872 513
Size: 552 1807 3435
Fragments arranged by size:
3435 1807 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5055
Fragments arranged by size:
5055 683 56
BtsI GCAGTG_nn'
Cuts at: 2344 3310 4104 4124 2344
Size: 966 794 20 4014
Fragments arranged by size:
4014 966 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5794
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5759
DraI TTT'AAA
Cuts at: 3098 3098
Size: 5794
DraIII CAC_nnn'GTG
Cuts at: 2124 3086 2124
Size: 962 4832
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4801 809
Size: 507 3485 1802
Fragments arranged by size:
3485 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2673 2853 3241
Size: 54 1425 50 275 18 843 180 388
Cuts at: 3241 4130 5759 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 843 388 275 180 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3241 1537
Size: 275 1429 4090
Fragments arranged by size:
4090 1429 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3724 1353
Size: 2371 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2501 4381 4755 4901 387
Size: 1064 138 912 1880 374 146 1280
Fragments arranged by size:
1880 1280 1064 912 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2062 2741 2903 3857 4454 5241 5509
Size: 393 430 679 162 954 597 787 268
Cuts at: 5509 1239
Size: 1524
Fragments arranged by size:
1524 954 787 679 597 430 393 268
162
EcoNI CCTnn'n_nnAGG
Cuts at: 2723 2816 2723
Size: 93 5701
EcoO109I rG'GnC_Cy
Cuts at: 2728 2729 2728
Size: 1 5793
EcoRI G'AATT_C
Cuts at: 3103 3481 3103
Size: 378 5416
FspI TGC'GCA
Cuts at: 4300 4300
Size: 5794
HaeII r_GCGC'y
Cuts at: 1678 4941 1678
Size: 3263 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2148 2180 2768 2800 3043
Size: 32 135 32 576 32 588 32 243
Cuts at: 3043 3075 4435 4467 4509 4541 1373
Size: 32 1360 32 42 32 2626
Fragments arranged by size:
2626 1360 588 576 243 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3138 3384 669
Size: 988 281 1200 246 3079
Fragments arranged by size:
3079 1200 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5794
HpaI GTT'AAC
Cuts at: 3384 3384
Size: 5794
KpnI G_GTAC'C
Cuts at: 2474 3119 2474
Size: 645 5149
MfeI C'AATT_G
Cuts at: 3393 3393
Size: 5794
MluI A'CGCG_T
Cuts at: 5670 5670
Size: 5794
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4908 5092 1305
Size: 3603 184 2007
Fragments arranged by size:
3603 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2675 2855 10
Size: 54 1425 343 843 180 2949
Fragments arranged by size:
2949 1425 843 343 180 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2096 2519 3752 4111 4270 518
Size: 648 843 87 423 1233 359 159 2042
Fragments arranged by size:
2042 1233 843 648 423 359 159 87
MspA1I CmG'CkG
Cuts at: 1606 3210 3887 5035 5280 1606
Size: 1604 677 1148 245 2120
Fragments arranged by size:
2120 1604 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5242
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5794
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5794
NotI GC'GGCC_GC
Cuts at: 3241 3241
Size: 5794
NspI r_CATG'y
Cuts at: 1132 1229 3152 4697 1132
Size: 97 1923 1545 2229
Fragments arranged by size:
2229 1923 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5794
PciI A'CATG_T
Cuts at: 4693 4693
Size: 5794
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5794
PfoI T'CCnGG_A
Cuts at: 2409 2718 2880 2409
Size: 309 162 5323
Fragments arranged by size:
5323 309 162
PmeI GTTT'AAAC
Cuts at: 3098 3098
Size: 5794
PmlI CAC'GTG
Cuts at: 2124 2124
Size: 5794
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3863 3895 5376 5408 3863
Size: 32 1481 32 4249
Fragments arranged by size:
4249 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5794
PsiI TTA'TAA
Cuts at: 3364 3364
Size: 5794
PspOMI G'GGCC_C
Cuts at: 2728 2728
Size: 5794
PstI C_TGCA'G
Cuts at: 830 3146 830
Size: 2316 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5794
SalI G'TCGA_C
Cuts at: 1936 3136 1936
Size: 1200 4594
SbfI CC_TGCA'GG
Cuts at: 3146 3146
Size: 5794
ScaI AGT'ACT
Cuts at: 4042 5754 4042
Size: 1712 4082
SfcI C'TryA_G
Cuts at: 826 1045 2398 3142 4277 4958 5149 826
Size: 219 1353 744 1135 681 191 1471
Fragments arranged by size:
1471 1353 1135 744 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5794
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5794
SmaI CCC'GGG
Cuts at: 1872 3121 1872
Size: 1249 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2147 2219 3898 4799 5061 820
Size: 197 926 204 72 1679 901 262 1553
Fragments arranged by size:
1679 1553 926 901 262 204 197 72
SnaBI TAC'GTA
Cuts at: 493 3585 493
Size: 3092 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5794
SphI G_CATG'C
Cuts at: 1132 3152 1132
Size: 2020 3774
SspI AAT'ATT
Cuts at: 5 52 3718 5
Size: 47 3666 2081
Fragments arranged by size:
3666 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5794
StyI C'CwwG_G
Cuts at: 513 1065 2298 2419 2562 2764 3233 513
Size: 552 1233 121 143 202 469 3074
Fragments arranged by size:
3074 1233 552 469 202 143 121
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2991 3821 3838 3991 4176 4621 325
Size: 1255 1411 830 17 153 185 445 1498
Fragments arranged by size:
1498 1411 1255 830 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2274 4040 5752
Size: 274 80 33 51 1443 297 1766 1712
Cuts at: 5752 96
Size: 138
Fragments arranged by size:
1766 1712 1443 297 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2318 2350 5754 5786 2318
Size: 32 3404 32 2326
Fragments arranged by size:
3404 2326 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2160 1316
Size: 844 4950
XbaI T'CTAG_A
Cuts at: 3130 3130
Size: 5794
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2305 2580 2974 2305
Size: 275 394 5125
Fragments arranged by size:
5125 394 275
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5794
XmaI C'CCGG_G
Cuts at: 1870 3119 1870
Size: 1249 4545
XmnI GAAnn'nnTTC
Cuts at: 2204 3923 2204
Size: 1719 4075
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3602 276
Size: 53 83 186 936 2068 2468
Fragments arranged by size:
2468 2068 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PpuMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI