Restriction map for
pFN21AB7400
(Circular) (Six-base) MAPSORT of: pFN21AB7400.seq Check: 9614 from: 1 to: 5620
pFN21AB7400 5620 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3430 278
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2229 2963 1937
Size: 292 734 4594
Fragments arranged by size:
4594 734 292
Acc65I G'GTAC_C
Cuts at: 2941 2941
Size: 5620
AclI AA'CG_TT
Cuts at: 1563 2489 3747 4120 1563
Size: 926 1258 373 3063
Fragments arranged by size:
3063 1258 926 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2283 3683 5067 5335 1632
Size: 651 1400 1384 268 1917
Fragments arranged by size:
1917 1400 1384 651 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5423
AflIII A'CryG_T
Cuts at: 1203 4519 5496 1203
Size: 3316 977 1327
Fragments arranged by size:
3316 1327 977
AgeI A'CCGG_T
Cuts at: 4451 4451
Size: 5620
AhdI GACnn_n'nnGTC
Cuts at: 4349 4349
Size: 5620
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5620
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5620
AlwNI CAG_nnn'CTG
Cuts at: 2030 3363 4935 2030
Size: 1333 1572 2715
Fragments arranged by size:
2715 1572 1333
ApaLI G'TGCA_C
Cuts at: 3677 4833 3677
Size: 1156 4464
ApoI r'AATT_y
Cuts at: 1493 2929 3155 3307 5416 1493
Size: 1436 226 152 2109 1697
Fragments arranged by size:
2109 1697 1436 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4174 160
Size: 872 3142 1606
Fragments arranged by size:
3142 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
AvaI C'yCGr_G
Cuts at: 1870 1943 2945 1870
Size: 73 1002 4545
Fragments arranged by size:
4545 1002 73
BamHI G'GATC_C
Cuts at: 2950 3317 2950
Size: 367 5253
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2941 4396 618
Size: 325 217 627 1154 1455 1842
Fragments arranged by size:
1842 1455 1154 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2939 1391
Size: 1548 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2271 928
Size: 971 372 4277
Fragments arranged by size:
4277 971 372
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2119 2153 3811 3845 1903
Size: 34 50 34 98 34 1658 34 3678
Fragments arranged by size:
3678 1658 98 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3512 4722 3512
Size: 1210 4410
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5620
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4231 136
Size: 107 122 71 631 605 2559 1525
Fragments arranged by size:
2559 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2789 5615 2789
Size: 2826 2794
BlpI GC'TnA_GC
Cuts at: 3037 3037
Size: 5620
Bme1580I G_kGCm'C
Cuts at: 1790 3681 4837 1790
Size: 1891 1156 2573
Fragments arranged by size:
2573 1891 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5620
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2912 3351 4309
Size: 416 53 83 399 24 1495 439 958
Cuts at: 4309 442
Size: 1753
Fragments arranged by size:
1753 1495 958 439 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5620
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4280 1239
Size: 3041 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2698 3745 4610 4908 2698
Size: 1047 865 298 3410
Fragments arranged by size:
3410 1047 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4283 882
Size: 617 2784 2219
Fragments arranged by size:
2784 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2371 3411 493
Size: 711 1167 1040 2702
Fragments arranged by size:
2702 1167 1040 711
BsaBI GATnn'nnATC
Cuts at: 5614 5614
Size: 5620
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3427 3809 4444 275
Size: 53 83 186 936 1894 382 635 1451
Fragments arranged by size:
1894 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4053 4451 4725 4872 5283 4053
Size: 398 274 147 411 4390
Fragments arranged by size:
4390 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2744 2774 1178
Size: 30 165 30 1341 30 4024
Fragments arranged by size:
4024 1341 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2026 2677 2680 2716 1186
Size: 840 651 3 36 4090
Fragments arranged by size:
4090 840 651 36 3
BseYI C'CCAG_C
Cuts at: 2044 2074 4823 5319 2044
Size: 30 2749 496 2345
Fragments arranged by size:
2749 2345 496 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5047
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3070 3831 4859 1540
Size: 275 186 1069 761 1028 2301
Fragments arranged by size:
2301 1069 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2640 3681 3766 4837 2640
Size: 1041 85 1071 3423
Fragments arranged by size:
3423 1071 1041 85
BsmI GAATG_Cn'
Cuts at: 2073 3131 3224 2073
Size: 1058 93 4469
Fragments arranged by size:
4469 1058 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2640 2939 3681 3766 4837 1391
Size: 399 850 299 742 85 1071 2174
Fragments arranged by size:
2174 1071 850 742 399 299 85
BspEI T'CCGG_A
Cuts at: 5283 5283
Size: 5620
BspHI T'CATG_A
Cuts at: 2862 3509 5239 2862
Size: 647 1730 3243
Fragments arranged by size:
3243 1730 647
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2261 2349 2975 844
Size: 1417 88 626 3489
Fragments arranged by size:
3489 1417 626 88
BsrBI CCG'CTC
Cuts at: 1394 1823 3507 1394
Size: 429 1684 3507
Fragments arranged by size:
3507 1684 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2426 4115 4289 65
Size: 1183 1178 1689 174 1396
Fragments arranged by size:
1689 1396 1183 1178 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4264 4451 1700
Size: 110 2454 187 2869
Fragments arranged by size:
2869 2454 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5620
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5620
BssSI C'ACGA_G
Cuts at: 3680 4692 3680
Size: 1012 4608
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5620
BstBI TT'CG_AA
Cuts at: 4424 4424
Size: 5620
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5620
BstYI r'GATC_y
Cuts at: 1972 2209 2241 2682 2789 2950 3317 3702 3719
Size: 237 32 441 107 161 367 385 17
Cuts at: 3719 5171 5257 5269 5615 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 441 385 367 346 237 161
107 86 32 17 12
Bsu36I CC'TnA_GG
Cuts at: 2876 2876
Size: 5620
BtgI C'CryG_G
Cuts at: 513 1065 2352 513
Size: 552 1287 3781
Fragments arranged by size:
3781 1287 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4881
Fragments arranged by size:
4881 683 56
BtsI GCAGTG_nn'
Cuts at: 2640 3136 3930 3950 2640
Size: 496 794 20 4310
Fragments arranged by size:
4310 794 496 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5620
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5585
DraI TTT'AAA
Cuts at: 2924 2924
Size: 5620
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4627 809
Size: 507 3311 1802
Fragments arranged by size:
3311 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2373 3067 3956
Size: 54 1425 50 275 18 543 694 889
Cuts at: 3956 5585 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 694 543 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3067 1537
Size: 275 1255 4090
Fragments arranged by size:
4090 1255 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2087 2473 3550 1353
Size: 734 386 1077 3423
Fragments arranged by size:
3423 1077 734 386
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4207 4581 4727 387
Size: 1064 138 2618 374 146 1280
Fragments arranged by size:
2618 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2283 3683 4280 5067 5335 1239
Size: 393 651 1400 597 787 268 1524
Fragments arranged by size:
1524 1400 787 651 597 393 268
EcoRI G'AATT_C
Cuts at: 2929 3307 2929
Size: 378 5242
FspI TGC'GCA
Cuts at: 4126 4126
Size: 5620
HaeII r_GCGC'y
Cuts at: 1678 4767 1678
Size: 3089 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2002 2034 4261 4293 4335
Size: 32 135 32 430 32 2227 32 42
Cuts at: 4335 4367 1373
Size: 32 2626
Fragments arranged by size:
2626 2227 430 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2964 3210 669
Size: 988 281 1026 246 3079
Fragments arranged by size:
3079 1026 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5620
HpaI GTT'AAC
Cuts at: 3210 3210
Size: 5620
KpnI G_GTAC'C
Cuts at: 2945 2945
Size: 5620
MfeI C'AATT_G
Cuts at: 3219 3219
Size: 5620
MluI A'CGCG_T
Cuts at: 5496 5496
Size: 5620
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4734 4918 1305
Size: 3429 184 2007
Fragments arranged by size:
3429 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2375 10
Size: 54 1425 343 543 3255
Fragments arranged by size:
3255 1425 543 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2174 3578 3937 4096 518
Size: 648 1008 1404 359 159 2042
Fragments arranged by size:
2042 1404 1008 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2074 3036 3713 4861 5106 1606
Size: 468 962 677 1148 245 2120
Fragments arranged by size:
2120 1148 962 677 468 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5068
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5620
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5620
NotI GC'GGCC_GC
Cuts at: 3067 3067
Size: 5620
NspI r_CATG'y
Cuts at: 1132 1229 2724 2978 4523 1132
Size: 97 1495 254 1545 2229
Fragments arranged by size:
2229 1545 1495 254 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5620
PciI A'CATG_T
Cuts at: 4519 4519
Size: 5620
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5620
PmeI GTTT'AAAC
Cuts at: 2924 2924
Size: 5620
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3689 3721 5202 5234 3689
Size: 32 1481 32 4075
Fragments arranged by size:
4075 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2741 1613
Size: 1128 4492
PsiI TTA'TAA
Cuts at: 3190 3190
Size: 5620
PstI C_TGCA'G
Cuts at: 830 2317 2972 830
Size: 1487 655 3478
Fragments arranged by size:
3478 1487 655
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5620
PvuII CAG'CTG
Cuts at: 2074 2074
Size: 5620
SalI G'TCGA_C
Cuts at: 1936 2962 1936
Size: 1026 4594
SbfI CC_TGCA'GG
Cuts at: 2972 2972
Size: 5620
ScaI AGT'ACT
Cuts at: 3868 5580 3868
Size: 1712 3908
SfcI C'TryA_G
Cuts at: 826 1045 2313 2326 2869 2968 4103 4784 4975
Size: 219 1268 13 543 99 1135 681 191
Cuts at: 4975 826
Size: 1471
Fragments arranged by size:
1471 1268 1135 681 543 219 191 99
13
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5620
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5620
SmaI CCC'GGG
Cuts at: 1872 2947 1872
Size: 1075 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2677 3724 4625 4887 820
Size: 197 926 734 1047 901 262 1553
Fragments arranged by size:
1553 1047 926 901 734 262 197
SnaBI TAC'GTA
Cuts at: 493 3411 493
Size: 2918 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5620
SphI G_CATG'C
Cuts at: 1132 2978 1132
Size: 1846 3774
SspI AAT'ATT
Cuts at: 5 52 3544 5
Size: 47 3492 2081
Fragments arranged by size:
3492 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5620
StyI C'CwwG_G
Cuts at: 513 1065 2550 2694 3059 513
Size: 552 1485 144 365 3074
Fragments arranged by size:
3074 1485 552 365 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2188 3647 3664 3817 4002 4447 325
Size: 1255 608 1459 17 153 185 445 1498
Fragments arranged by size:
1498 1459 1255 608 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2103 2598 3866
Size: 274 80 33 51 1443 126 495 1268
Cuts at: 3866 5578 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1268 495 274 138 126 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5580 5612 5580
Size: 32 5588
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5620
XbaI T'CTAG_A
Cuts at: 2956 2956
Size: 5620
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5620
XmaI C'CCGG_G
Cuts at: 1870 2945 1870
Size: 1075 4545
XmnI GAAnn'nnTTC
Cuts at: 3749 3749
Size: 5620
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3428 276
Size: 53 83 186 936 1894 2468
Fragments arranged by size:
2468 1894 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI