Restriction map for
pFN21AB7393
(Circular) (Six-base) MAPSORT of: pFN21AB7393.seq Check: 3772 from: 1 to: 5947
pFN21AB7393 5947 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3757 278
Size: 53 83 186 936 2221 2468
Fragments arranged by size:
2468 2221 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3290 1937
Size: 1353 4594
Acc65I G'GTAC_C
Cuts at: 3268 3268
Size: 5947
AclI AA'CG_TT
Cuts at: 1563 4074 4447 1563
Size: 2511 373 3063
Fragments arranged by size:
3063 2511 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2180 2373 2465 3201 4010 5394 5662 1632
Size: 548 193 92 736 809 1384 268 1917
Fragments arranged by size:
1917 1384 809 736 548 268 193 92
AflII C'TTAA_G
Cuts at: 820 1017 2756 820
Size: 197 1739 4011
Fragments arranged by size:
4011 1739 197
AflIII A'CryG_T
Cuts at: 1203 4846 5823 1203
Size: 3643 977 1327
Fragments arranged by size:
3643 1327 977
AgeI A'CCGG_T
Cuts at: 4778 4778
Size: 5947
AhdI GACnn_n'nnGTC
Cuts at: 4676 4676
Size: 5947
AleI CACnn'nnGTG
Cuts at: 1166 2378 1166
Size: 1212 4735
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3156 1184
Size: 1972 3975
AlwNI CAG_nnn'CTG
Cuts at: 3690 5262 3690
Size: 1572 4375
ApaLI G'TGCA_C
Cuts at: 4004 5160 4004
Size: 1156 4791
ApoI r'AATT_y
Cuts at: 1493 2219 2393 2455 2761 2873 2881 3069 3256
Size: 726 174 62 306 112 8 188 187
Cuts at: 3256 3482 3634 5743 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 726 306 226 188 187 174
152 112 62 8
AseI AT'TA_AT
Cuts at: 160 1032 4501 160
Size: 872 3469 1606
Fragments arranged by size:
3469 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
AvaI C'yCGr_G
Cuts at: 1870 1943 3272 1870
Size: 73 1329 4545
Fragments arranged by size:
4545 1329 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2346 2379 2346
Size: 33 5914
BamHI G'GATC_C
Cuts at: 3277 3644 3277
Size: 367 5580
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3268 4723 618
Size: 325 217 627 1481 1455 1842
Fragments arranged by size:
1842 1481 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3266 1391
Size: 1875 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2390 928
Size: 971 491 4485
Fragments arranged by size:
4485 971 491
BbvCI CC'TCA_GC
Cuts at: 2702 2702
Size: 5947
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4138 4172 1903
Size: 34 2201 34 3678
Fragments arranged by size:
3678 2201 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3052 3839 5049 3052
Size: 787 1210 3950
Fragments arranged by size:
3950 1210 787
BclI T'GATC_A
Cuts at: 1548 2031 1548
Size: 483 5464
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4558 136
Size: 107 122 71 631 605 2886 1525
Fragments arranged by size:
2886 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5942 5942
Size: 5947
BlpI GC'TnA_GC
Cuts at: 3364 3364
Size: 5947
Bme1580I G_kGCm'C
Cuts at: 1790 4008 5164 1790
Size: 2218 1156 2573
Fragments arranged by size:
2573 2218 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5947
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3678 4636 442
Size: 416 53 83 399 24 2261 958 1753
Fragments arranged by size:
2261 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5947
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2349 2349
Size: 5947
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2660 2732 4607 1239
Size: 1421 72 1875 2579
Fragments arranged by size:
2579 1875 1421 72
Bpu10I CC'TnA_GC
Cuts at: 2060 2189 2702 2060
Size: 129 513 5305
Fragments arranged by size:
5305 513 129
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2575 4072 4937 5235 2575
Size: 1497 865 298 3287
Fragments arranged by size:
3287 1497 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2589 4610 882
Size: 617 1090 2021 2219
Fragments arranged by size:
2219 2021 1090 617
BsaAI yAC'GTr
Cuts at: 493 1204 3738 493
Size: 711 2534 2702
Fragments arranged by size:
2702 2534 711
BsaBI GATnn'nnATC
Cuts at: 5941 5941
Size: 5947
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2079 3754 4136 4771
Size: 53 83 186 936 546 1675 382 635
Cuts at: 4771 275
Size: 1451
Fragments arranged by size:
1675 1451 936 635 546 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4380 4778 5052 5199 5610 4380
Size: 398 274 147 411 4717
Fragments arranged by size:
4717 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5722
Fragments arranged by size:
5722 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5947
BseYI C'CCAG_C
Cuts at: 5150 5646 5150
Size: 496 5451
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2753 2970 1163
Size: 573 1017 217 4140
Fragments arranged by size:
4140 1017 573 217
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3397 4158 5186 1540
Size: 275 186 1396 761 1028 2301
Fragments arranged by size:
2301 1396 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4008 4093 5164 4008
Size: 85 1071 4791
Fragments arranged by size:
4791 1071 85
BsmI GAATG_Cn'
Cuts at: 3133 3458 3551 3133
Size: 325 93 5529
Fragments arranged by size:
5529 325 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3266 4008 4093 5164 1391
Size: 399 1476 742 85 1071 2174
Fragments arranged by size:
2174 1476 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5610 5610
Size: 5947
BspHI T'CATG_A
Cuts at: 3836 5566 3836
Size: 1730 4217
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3302 844
Size: 2458 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3834 1394
Size: 429 2011 3507
Fragments arranged by size:
3507 2011 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4442 4616 65
Size: 1183 3194 174 1396
Fragments arranged by size:
3194 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2037 2128 4591 4778 1700
Size: 110 227 91 2463 187 2869
Fragments arranged by size:
2869 2463 227 187 110 91
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5947
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5947
BssSI C'ACGA_G
Cuts at: 4007 5019 4007
Size: 1012 4935
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5947
BstBI TT'CG_AA
Cuts at: 4751 4751
Size: 5947
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5947
BstYI r'GATC_y
Cuts at: 1972 2902 3277 3644 4029 4046 5498 5584 5596
Size: 930 375 367 385 17 1452 86 12
Cuts at: 5596 5942 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 930 385 375 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2540 2540
Size: 5947
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5395
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5208
Fragments arranged by size:
5208 683 56
BtsI GCAGTG_nn'
Cuts at: 3463 4257 4277 3463
Size: 794 20 5133
Fragments arranged by size:
5133 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5947
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5912
DraI TTT'AAA
Cuts at: 2304 2407 2766 3251 2304
Size: 103 359 485 5000
Fragments arranged by size:
5000 485 359 103
DraIII CAC_nnn'GTG
Cuts at: 3151 3151
Size: 5947
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4954 809
Size: 507 3638 1802
Fragments arranged by size:
3638 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3394 4283 5912
Size: 54 1425 50 275 18 1564 889 1629
Cuts at: 5912 8
Size: 43
Fragments arranged by size:
1629 1564 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3394 1537
Size: 275 1582 4090
Fragments arranged by size:
4090 1582 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3877 1353
Size: 2524 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4534 4908 5054 387
Size: 1064 138 2945 374 146 1280
Fragments arranged by size:
2945 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2180 2373 2465 2660 2732 3201 4010
Size: 393 548 193 92 195 72 469 809
Cuts at: 4010 4607 5394 5662 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 809 787 597 548 469 393 268
195 193 92 72
EcoO109I rG'GnC_Cy
Cuts at: 2175 2175
Size: 5947
EcoRI G'AATT_C
Cuts at: 3256 3634 3256
Size: 378 5569
EcoRV GAT'ATC
Cuts at: 2272 2272
Size: 5947
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2330 2330
Size: 5947
FspI TGC'GCA
Cuts at: 4453 4453
Size: 5947
HaeII r_GCGC'y
Cuts at: 1678 5094 1678
Size: 3416 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2086 2118 4588 4620 4662
Size: 32 135 32 514 32 2470 32 42
Cuts at: 4662 4694 1373
Size: 32 2626
Fragments arranged by size:
2626 2470 514 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3291 3537 669
Size: 988 281 1353 246 3079
Fragments arranged by size:
3079 1353 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2576 2852 748
Size: 1828 276 3843
Fragments arranged by size:
3843 1828 276
HpaI GTT'AAC
Cuts at: 3537 3537
Size: 5947
KpnI G_GTAC'C
Cuts at: 3272 3272
Size: 5947
MfeI C'AATT_G
Cuts at: 2589 3546 2589
Size: 957 4990
MluI A'CGCG_T
Cuts at: 5823 5823
Size: 5947
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5061 5245 1305
Size: 3756 184 2007
Fragments arranged by size:
3756 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4125
Fragments arranged by size:
4125 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2378 3905 4264 4423 518
Size: 648 1212 1527 359 159 2042
Fragments arranged by size:
2042 1527 1212 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3363 4040 5188 5433 1606
Size: 1757 677 1148 245 2120
Fragments arranged by size:
2120 1757 1148 677 245
NaeI GCC'GGC
Cuts at: 2039 2039
Size: 5947
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5395
NdeI CA'TA_TG
Cuts at: 387 3016 387
Size: 2629 3318
NgoMIV G'CCGG_C
Cuts at: 2037 2037
Size: 5947
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5947
NotI GC'GGCC_GC
Cuts at: 3394 3394
Size: 5947
NspI r_CATG'y
Cuts at: 1132 1229 3305 4850 1132
Size: 97 2076 1545 2229
Fragments arranged by size:
2229 2076 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5947
PciI A'CATG_T
Cuts at: 4846 4846
Size: 5947
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3055 3151 1108
Size: 1947 96 3904
Fragments arranged by size:
3904 1947 96
PfoI T'CCnGG_A
Cuts at: 2114 2748 2114
Size: 634 5313
PmeI GTTT'AAAC
Cuts at: 3251 3251
Size: 5947
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4016 4048 5529 5561 4016
Size: 32 1481 32 4402
Fragments arranged by size:
4402 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2175 2175
Size: 5947
PshAI GACnn'nnGTC
Cuts at: 1613 2556 1613
Size: 943 5004
PsiI TTA'TAA
Cuts at: 3517 3517
Size: 5947
PstI C_TGCA'G
Cuts at: 830 3299 830
Size: 2469 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5947
RsrII CG'GwC_CG
Cuts at: 2131 2131
Size: 5947
SalI G'TCGA_C
Cuts at: 1936 3289 1936
Size: 1353 4594
SanDI GG'GwC_CC
Cuts at: 2175 2175
Size: 5947
SbfI CC_TGCA'GG
Cuts at: 3299 3299
Size: 5947
ScaI AGT'ACT
Cuts at: 4195 5907 4195
Size: 1712 4235
SfcI C'TryA_G
Cuts at: 826 1045 3295 4430 5111 5302 826
Size: 219 2250 1135 681 191 1471
Fragments arranged by size:
2250 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5947
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5947
SmaI CCC'GGG
Cuts at: 1872 3274 1872
Size: 1402 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2554 2756 4051 4952 5214 820
Size: 197 926 611 202 1295 901 262 1553
Fragments arranged by size:
1553 1295 926 901 611 262 202 197
SnaBI TAC'GTA
Cuts at: 493 3738 493
Size: 3245 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5947
SphI G_CATG'C
Cuts at: 1132 3305 1132
Size: 2173 3774
SspI AAT'ATT
Cuts at: 5 52 3871 5
Size: 47 3819 2081
Fragments arranged by size:
3819 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5947
StyI C'CwwG_G
Cuts at: 513 1065 2052 2469 3386 513
Size: 552 987 417 917 3074
Fragments arranged by size:
3074 987 917 552 417
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3161 3974 3991 4144 4329 4774 325
Size: 1255 1581 813 17 153 185 445 1498
Fragments arranged by size:
1581 1498 1255 813 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4193 5905 96
Size: 274 80 33 51 1443 2216 1712 138
Fragments arranged by size:
2216 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5907 5939 5907
Size: 32 5915
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5947
XbaI T'CTAG_A
Cuts at: 3283 3283
Size: 5947
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5947
XmaI C'CCGG_G
Cuts at: 1870 3272 1870
Size: 1402 4545
XmnI GAAnn'nnTTC
Cuts at: 2478 2787 3101 4076 2478
Size: 309 314 975 4349
Fragments arranged by size:
4349 975 314 309
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3755 276
Size: 53 83 186 936 2221 2468
Fragments arranged by size:
2468 2221 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
RsrII SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI FseI FspAI
KasI NarI NruI NsiI PacI PmlI PspOMI PspXI
PsrI PvuII SacI SacII SapI SexAI SfiI SfoI
SrfI SwaI XcmI