Restriction map for
pFN21AB7386
(Circular) (Six-base) MAPSORT of: pFN21AB7386.seq Check: 5011 from: 1 to: 5959
pFN21AB7386 5959 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2086 2086
Size: 5959
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3769 278
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3302 1937
Size: 1365 4594
Acc65I G'GTAC_C
Cuts at: 3280 3280
Size: 5959
AclI AA'CG_TT
Cuts at: 1563 4086 4459 1563
Size: 2523 373 3063
Fragments arranged by size:
3063 2523 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2529 2640 4022 5406 5674 1632
Size: 897 111 1382 1384 268 1917
Fragments arranged by size:
1917 1384 1382 897 268 111
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5762
AflIII A'CryG_T
Cuts at: 1203 4858 5835 1203
Size: 3655 977 1327
Fragments arranged by size:
3655 1327 977
AgeI A'CCGG_T
Cuts at: 4790 4790
Size: 5959
AhdI GACnn_n'nnGTC
Cuts at: 4688 4688
Size: 5959
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5959
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5959
AlwNI CAG_nnn'CTG
Cuts at: 2305 2663 3038 3702 5274 2305
Size: 358 375 664 1572 2990
Fragments arranged by size:
2990 1572 664 375 358
ApaLI G'TGCA_C
Cuts at: 3220 4016 5172 3220
Size: 796 1156 4007
Fragments arranged by size:
4007 1156 796
ApoI r'AATT_y
Cuts at: 1493 2138 2498 2611 2798 3268 3494 3646 5755
Size: 645 360 113 187 470 226 152 2109
Cuts at: 5755 1493
Size: 1697
Fragments arranged by size:
2109 1697 645 470 360 226 187 152
113
AseI AT'TA_AT
Cuts at: 160 1032 4513 160
Size: 872 3481 1606
Fragments arranged by size:
3481 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
AvaI C'yCGr_G
Cuts at: 1870 1943 3284 1870
Size: 73 1341 4545
Fragments arranged by size:
4545 1341 73
BamHI G'GATC_C
Cuts at: 2630 3289 3656 2630
Size: 659 367 4933
Fragments arranged by size:
4933 659 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3190 3280 4735 618
Size: 325 217 627 1403 90 1455 1842
Fragments arranged by size:
1842 1455 1403 627 325 217 90
BanII G_rGCy'C
Cuts at: 1391 3278 1391
Size: 1887 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2025 2919 928
Size: 971 126 894 3968
Fragments arranged by size:
3968 971 894 126
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4150 4184 1903
Size: 34 2213 34 3678
Fragments arranged by size:
3678 2213 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3851 5061 3851
Size: 1210 4749
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5959
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4570 136
Size: 107 122 71 631 605 2898 1525
Fragments arranged by size:
2898 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5954 5954
Size: 5959
BlpI GC'TnA_GC
Cuts at: 3376 3376
Size: 5959
Bme1580I G_kGCm'C
Cuts at: 1790 3224 4020 5176 1790
Size: 1434 796 1156 2573
Fragments arranged by size:
2573 1434 1156 796
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5959
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3690 4648 442
Size: 416 53 83 399 24 2273 958 1753
Fragments arranged by size:
2273 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2415 4619 1239
Size: 1176 2204 2579
Fragments arranged by size:
2579 2204 1176
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2305 2679 4084 4949 5247 2305
Size: 374 1405 865 298 3017
Fragments arranged by size:
3017 1405 865 374 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4622 882
Size: 617 3123 2219
Fragments arranged by size:
3123 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3750 493
Size: 711 2546 2702
Fragments arranged by size:
2702 2546 711
BsaBI GATnn'nnATC
Cuts at: 5953 5953
Size: 5959
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3766 4148 4783 275
Size: 53 83 186 936 2233 382 635 1451
Fragments arranged by size:
2233 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4392 4790 5064 5211 5622 4392
Size: 398 274 147 411 4729
Fragments arranged by size:
4729 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5734
Fragments arranged by size:
5734 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2944 2947 1186
Size: 1758 3 4198
Fragments arranged by size:
4198 1758 3
BseYI C'CCAG_C
Cuts at: 5162 5658 5162
Size: 496 5463
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2120 2688 1163
Size: 573 384 568 4434
Fragments arranged by size:
4434 573 568 384
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3409 4170 5198 1540
Size: 275 186 1408 761 1028 2301
Fragments arranged by size:
2301 1408 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3224 4020 4105 5176 3224
Size: 796 85 1071 4007
Fragments arranged by size:
4007 1071 796 85
BsmI GAATG_Cn'
Cuts at: 2110 2155 2872 3470 3563 2110
Size: 45 717 598 93 4506
Fragments arranged by size:
4506 717 598 93 45
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3224 3278 4020 4105 5176 1391
Size: 399 1434 54 742 85 1071 2174
Fragments arranged by size:
2174 1434 1071 742 399 85 54
BspEI T'CCGG_A
Cuts at: 5622 5622
Size: 5959
BspHI T'CATG_A
Cuts at: 3848 5578 3848
Size: 1730 4229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2086 2100 3314 844
Size: 1242 14 1214 3489
Fragments arranged by size:
3489 1242 1214 14
BsrBI CCG'CTC
Cuts at: 1394 1823 3846 1394
Size: 429 2023 3507
Fragments arranged by size:
3507 2023 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2115 3237 4454 4628 65
Size: 1183 867 1122 1217 174 1396
Fragments arranged by size:
1396 1217 1183 1122 867 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4603 4790 1700
Size: 110 2793 187 2869
Fragments arranged by size:
2869 2793 187 110
BsrGI T'GTAC_A
Cuts at: 96 2998 96
Size: 2902 3057
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5959
BssSI C'ACGA_G
Cuts at: 4019 5031 4019
Size: 1012 4947
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5959
BstBI TT'CG_AA
Cuts at: 4763 4763
Size: 5959
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5959
BstYI r'GATC_y
Cuts at: 1972 2053 2630 3289 3656 4041 4058 5510 5596
Size: 81 577 659 367 385 17 1452 86
Cuts at: 5596 5608 5954 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 659 577 385 367 346 86
81 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2682 513
Size: 552 1617 3790
Fragments arranged by size:
3790 1617 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5220
Fragments arranged by size:
5220 683 56
BtsI GCAGTG_nn'
Cuts at: 3222 3475 4269 4289 3222
Size: 253 794 20 4892
Fragments arranged by size:
4892 794 253 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5959
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5924
DraI TTT'AAA
Cuts at: 2317 2503 2748 3263 2317
Size: 186 245 515 5013
Fragments arranged by size:
5013 515 245 186
DraIII CAC_nnn'GTG
Cuts at: 2897 2897
Size: 5959
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4966 809
Size: 507 3650 1802
Fragments arranged by size:
3650 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2808 3406 4295
Size: 54 1425 50 275 18 978 598 889
Cuts at: 4295 5924 8
Size: 1629 43
Fragments arranged by size:
1629 1425 978 889 598 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3406 1537
Size: 275 1594 4090
Fragments arranged by size:
4090 1594 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2175 2520 2593 2965 3230 3235 3889 1353
Size: 822 345 73 372 265 5 654 3423
Fragments arranged by size:
3423 822 654 372 345 265 73 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4546 4920 5066 387
Size: 1064 138 2957 374 146 1280
Fragments arranged by size:
2957 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2415 2529 2640 4022 4619 5406 5674
Size: 393 783 114 111 1382 597 787 268
Cuts at: 5674 1239
Size: 1524
Fragments arranged by size:
1524 1382 787 783 597 393 268 114
111
EcoRI G'AATT_C
Cuts at: 2138 2611 3268 3646 2138
Size: 473 657 378 4451
Fragments arranged by size:
4451 657 473 378
FspI TGC'GCA
Cuts at: 4465 4465
Size: 5959
HaeII r_GCGC'y
Cuts at: 1678 5106 1678
Size: 3428 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2354 2386 2464 2496 4600
Size: 32 135 32 782 32 78 32 2104
Cuts at: 4600 4632 4674 4706 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2104 782 135 78 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3303 3549 669
Size: 988 281 1365 246 3079
Fragments arranged by size:
3079 1365 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2217 748
Size: 1469 4490
HpaI GTT'AAC
Cuts at: 3549 3549
Size: 5959
KpnI G_GTAC'C
Cuts at: 3284 3284
Size: 5959
MfeI C'AATT_G
Cuts at: 3558 3558
Size: 5959
MluI A'CGCG_T
Cuts at: 5835 5835
Size: 5959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2480 3157 5073 5257 1305
Size: 1175 677 1916 184 2007
Fragments arranged by size:
2007 1916 1175 677 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2810 10
Size: 54 1425 343 978 3159
Fragments arranged by size:
3159 1425 978 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2075 3917 4276 4435 518
Size: 648 909 1842 359 159 2042
Fragments arranged by size:
2042 1842 909 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2690 3375 4052 5200 5445 1606
Size: 1084 685 677 1148 245 2120
Fragments arranged by size:
2120 1148 1084 685 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2682 513
Size: 552 1617 3790
Fragments arranged by size:
3790 1617 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5959
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5959
NotI GC'GGCC_GC
Cuts at: 3406 3406
Size: 5959
NspI r_CATG'y
Cuts at: 1132 1229 3317 4862 1132
Size: 97 2088 1545 2229
Fragments arranged by size:
2229 2088 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5959
PciI A'CATG_T
Cuts at: 4858 4858
Size: 5959
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2626 1108
Size: 1518 4441
PmeI GTTT'AAAC
Cuts at: 3263 3263
Size: 5959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4028 4060 5541 5573 4028
Size: 32 1481 32 4414
Fragments arranged by size:
4414 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5959
PsiI TTA'TAA
Cuts at: 3529 3529
Size: 5959
PstI C_TGCA'G
Cuts at: 830 2097 3311 830
Size: 1267 1214 3478
Fragments arranged by size:
3478 1267 1214
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5959
PvuII CAG'CTG
Cuts at: 2690 2690
Size: 5959
SalI G'TCGA_C
Cuts at: 1936 3301 1936
Size: 1365 4594
SapI GCTCTTCn'nnn_
Cuts at: 2175 2520 2175
Size: 345 5614
SbfI CC_TGCA'GG
Cuts at: 2097 3311 2097
Size: 1214 4745
ScaI AGT'ACT
Cuts at: 4207 5919 4207
Size: 1712 4247
SfcI C'TryA_G
Cuts at: 826 1045 2093 2709 3024 3150 3307 4442 5123
Size: 219 1048 616 315 126 157 1135 681
Cuts at: 5123 5314 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1048 681 616 315 219 191
157 126
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5959
SmaI CCC'GGG
Cuts at: 1872 3286 1872
Size: 1414 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2284 2694 4063 4964 5226 820
Size: 197 926 341 410 1369 901 262 1553
Fragments arranged by size:
1553 1369 926 901 410 341 262 197
SnaBI TAC'GTA
Cuts at: 493 3750 493
Size: 3257 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5959
SphI G_CATG'C
Cuts at: 1132 3317 1132
Size: 2185 3774
SspI AAT'ATT
Cuts at: 5 52 2358 3883 5
Size: 47 2306 1525 2081
Fragments arranged by size:
2306 2081 1525 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5959
StyI C'CwwG_G
Cuts at: 513 1065 2682 3398 513
Size: 552 1617 716 3074
Fragments arranged by size:
3074 1617 716 552
SwaI ATTT'AAAT
Cuts at: 2503 2503
Size: 5959
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3986 4003 4156 4341 4786 325
Size: 1255 2406 17 153 185 445 1498
Fragments arranged by size:
2406 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2998 4205 5917
Size: 274 80 33 51 1443 1021 1207 1712
Cuts at: 5917 96
Size: 138
Fragments arranged by size:
1712 1443 1207 1021 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5919 5951 5919
Size: 32 5927
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5959
XbaI T'CTAG_A
Cuts at: 3295 3295
Size: 5959
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3201 3201
Size: 5959
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5959
XmaI C'CCGG_G
Cuts at: 1870 3284 1870
Size: 1414 4545
XmnI GAAnn'nnTTC
Cuts at: 2514 4088 2514
Size: 1574 4385
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3767 276
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI