Restriction map for
pFN21AB7384
(Circular) (Six-base) MAPSORT of: pFN21AB7384.seq Check: 8015 from: 1 to: 6097
pFN21AB7384 6097 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3907 278
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3440 1937
Size: 1503 4594
Acc65I G'GTAC_C
Cuts at: 2651 3167 3418 2651
Size: 516 251 5330
Fragments arranged by size:
5330 516 251
AclI AA'CG_TT
Cuts at: 1563 4224 4597 1563
Size: 2661 373 3063
Fragments arranged by size:
3063 2661 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4160 5544 5812 1632
Size: 2528 1384 268 1917
Fragments arranged by size:
2528 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5900
AflIII A'CryG_T
Cuts at: 1203 2208 4996 5973 1203
Size: 1005 2788 977 1327
Fragments arranged by size:
2788 1327 1005 977
AgeI A'CCGG_T
Cuts at: 4928 4928
Size: 6097
AhdI GACnn_n'nnGTC
Cuts at: 4826 4826
Size: 6097
AleI CACnn'nnGTG
Cuts at: 1166 3240 1166
Size: 2074 4023
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6097
AlwNI CAG_nnn'CTG
Cuts at: 3840 5412 3840
Size: 1572 4525
ApaLI G'TGCA_C
Cuts at: 4154 5310 4154
Size: 1156 4941
ApoI r'AATT_y
Cuts at: 1493 2472 3387 3406 3632 3784 5893 1493
Size: 979 915 19 226 152 2109 1697
Fragments arranged by size:
2109 1697 979 915 226 152 19
AseI AT'TA_AT
Cuts at: 160 1032 4651 160
Size: 872 3619 1606
Fragments arranged by size:
3619 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
AvaI C'yCGr_G
Cuts at: 1870 1943 3422 1870
Size: 73 1479 4545
Fragments arranged by size:
4545 1479 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2635 2643 2668 2676 2635
Size: 8 25 8 6056
Fragments arranged by size:
6056 25 8 8
BamHI G'GATC_C
Cuts at: 3427 3794 3427
Size: 367 5730
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2131 2651 3167 3418 4873
Size: 325 217 627 344 520 516 251 1455
Cuts at: 4873 618
Size: 1842
Fragments arranged by size:
1842 1455 627 520 516 344 325 251
217
BanII G_rGCy'C
Cuts at: 1391 2244 2622 3416 1391
Size: 853 378 794 4072
Fragments arranged by size:
4072 853 794 378
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2895 3017 928
Size: 971 996 122 4008
Fragments arranged by size:
4008 996 971 122
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4288 4322 1903
Size: 34 2351 34 3678
Fragments arranged by size:
3678 2351 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3989 5199 3989
Size: 1210 4887
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6097
BfrBI ATG'CAT
Cuts at: 2587 2587
Size: 6097
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4708 136
Size: 107 122 71 631 605 337 2699 1525
Fragments arranged by size:
2699 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 3324 6092 3324
Size: 2768 3329
BlpI GC'TnA_GC
Cuts at: 3514 3514
Size: 6097
Bme1580I G_kGCm'C
Cuts at: 1790 2136 4158 5314 1790
Size: 346 2022 1156 2573
Fragments arranged by size:
2573 2022 1156 346
BmgBI CAC'GTC
Cuts at: 1318 2149 1318
Size: 831 5266
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3828 4786 442
Size: 416 53 83 399 24 2411 958 1753
Fragments arranged by size:
2411 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6097
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2315 2317 2335 2315
Size: 2 18 6077
Fragments arranged by size:
6077 18 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3201 4757 1239
Size: 1962 1556 2579
Fragments arranged by size:
2579 1962 1556
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4222 5087 5385 4222
Size: 865 298 4934
Fragments arranged by size:
4934 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4760 882
Size: 617 3261 2219
Fragments arranged by size:
3261 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2211 3888 493
Size: 711 1007 1677 2702
Fragments arranged by size:
2702 1677 1007 711
BsaBI GATnn'nnATC
Cuts at: 6091 6091
Size: 6097
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3904 4286 4921 275
Size: 53 83 186 936 2371 382 635 1451
Fragments arranged by size:
2371 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2094 4530 4928 5202 5349 5760 2094
Size: 2436 398 274 147 411 2431
Fragments arranged by size:
2436 2431 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5872
Fragments arranged by size:
5872 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2689 3033 3172 1186
Size: 1503 344 139 4111
Fragments arranged by size:
4111 1503 344 139
BseYI C'CCAG_C
Cuts at: 2135 5300 5796 2135
Size: 3165 496 2436
Fragments arranged by size:
3165 2436 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5524
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2064 2167 3547 4308 5336 1540
Size: 275 186 63 103 1380 761 1028 2301
Fragments arranged by size:
2301 1380 1028 761 275 186 103 63
BsiHKAI G_wGCw'C
Cuts at: 2148 2316 2569 2622 4158 4243 5314 2148
Size: 168 253 53 1536 85 1071 2931
Fragments arranged by size:
2931 1536 1071 253 168 85 53
BsmI GAATG_Cn'
Cuts at: 3608 3701 3608
Size: 93 6004
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2136 2148 2244 2316 2569 2622 3416
Size: 399 346 12 96 72 253 53 794
Cuts at: 3416 4158 4243 5314 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 794 742 399 346 253 96
85 72 53 12
BspEI T'CCGG_A
Cuts at: 2094 5760 2094
Size: 3666 2431
BspHI T'CATG_A
Cuts at: 3986 5716 3986
Size: 1730 4367
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3452 844
Size: 2608 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3984 1394
Size: 429 2161 3507
Fragments arranged by size:
3507 2161 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4592 4766 65
Size: 1183 3344 174 1396
Fragments arranged by size:
3344 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4741 4928 1700
Size: 110 2931 187 2869
Fragments arranged by size:
2931 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6097
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6097
BssSI C'ACGA_G
Cuts at: 4157 5169 4157
Size: 1012 5085
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6097
BstBI TT'CG_AA
Cuts at: 4901 4901
Size: 6097
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6097
BstXI CCAn_nnnn'nTGG
Cuts at: 2648 2648
Size: 6097
BstYI r'GATC_y
Cuts at: 1972 2295 3324 3427 3794 4179 4196 5648 5734
Size: 323 1029 103 367 385 17 1452 86
Cuts at: 5734 5746 6092 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1029 385 367 346 323 103
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2280 513
Size: 552 1215 4330
Fragments arranged by size:
4330 1215 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2030 487
Size: 683 56 804 4554
Fragments arranged by size:
4554 804 683 56
BtsI GCAGTG_nn'
Cuts at: 3613 4407 4427 3613
Size: 794 20 5283
Fragments arranged by size:
5283 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6097
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6062
DraI TTT'AAA
Cuts at: 3401 3401
Size: 6097
DraIII CAC_nnn'GTG
Cuts at: 2121 2121
Size: 6097
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5104 809
Size: 507 3788 1802
Fragments arranged by size:
3788 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2061 2164 2283
Size: 54 1425 50 275 18 231 103 119
Cuts at: 2283 3289 3544 4433 6062 8
Size: 1006 255 889 1629 43
Fragments arranged by size:
1629 1425 1006 889 275 255 231 119
103 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2061 2164 3544 1537
Size: 275 249 103 1380 4090
Fragments arranged by size:
4090 1380 275 249 103
EarI CTCTTCn'nnn_
Cuts at: 1353 2232 2622 3190 3305 4027 1353
Size: 879 390 568 115 722 3423
Fragments arranged by size:
3423 879 722 568 390 115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4684 5058 5204 387
Size: 1064 138 3095 374 146 1280
Fragments arranged by size:
3095 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2620 2620
Size: 6097
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3201 4160 4757 5544 5812 1239
Size: 393 1569 959 597 787 268 1524
Fragments arranged by size:
1569 1524 959 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3117 3117
Size: 6097
EcoO109I rG'GnC_Cy
Cuts at: 2416 2416
Size: 6097
EcoRI G'AATT_C
Cuts at: 2472 3406 3784 2472
Size: 934 378 4785
Fragments arranged by size:
4785 934 378
EcoRV GAT'ATC
Cuts at: 2736 2736
Size: 6097
FspI TGC'GCA
Cuts at: 4603 4603
Size: 6097
HaeII r_GCGC'y
Cuts at: 1678 5244 1678
Size: 3566 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4738 4770 4812 4844 1373
Size: 32 135 32 3166 32 42 32 2626
Fragments arranged by size:
3166 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2110 2560 2902 2968 3441 3687
Size: 988 281 172 450 342 66 473 246
Cuts at: 3687 669
Size: 3079
Fragments arranged by size:
3079 988 473 450 342 281 246 172
66
HindIII A'AGCT_T
Cuts at: 748 2662 748
Size: 1914 4183
HpaI GTT'AAC
Cuts at: 2968 3687 2968
Size: 719 5378
KpnI G_GTAC'C
Cuts at: 2655 3171 3422 2655
Size: 516 251 5330
Fragments arranged by size:
5330 516 251
MfeI C'AATT_G
Cuts at: 3696 3696
Size: 6097
MluI A'CGCG_T
Cuts at: 5973 5973
Size: 6097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2425 2849 5211 5395 1305
Size: 1120 424 2362 184 2007
Fragments arranged by size:
2362 2007 1120 424 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2285 3291 10
Size: 54 1425 343 453 1006 2816
Fragments arranged by size:
2816 1425 1006 453 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2646 3240 4055 4414 4573 518
Size: 648 1480 594 815 359 159 2042
Fragments arranged by size:
2042 1480 815 648 594 359 159
MspA1I CmG'CkG
Cuts at: 1606 2306 3368 3513 4190 5338 5583 1606
Size: 700 1062 145 677 1148 245 2120
Fragments arranged by size:
2120 1148 1062 700 677 245 145
NcoI C'CATG_G
Cuts at: 513 1065 2280 513
Size: 552 1215 4330
Fragments arranged by size:
4330 1215 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6097
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6097
NotI GC'GGCC_GC
Cuts at: 2061 3544 2061
Size: 1483 4614
NsiI A_TGCA'T
Cuts at: 2589 2589
Size: 6097
NspI r_CATG'y
Cuts at: 1132 1229 3455 5000 1132
Size: 97 2226 1545 2229
Fragments arranged by size:
2229 2226 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2359 1348
Size: 1011 5086
PciI A'CATG_T
Cuts at: 4996 4996
Size: 6097
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2978 1108
Size: 1870 4227
PmeI GTTT'AAAC
Cuts at: 3401 3401
Size: 6097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4166 4198 5679 5711 4166
Size: 32 1481 32 4552
Fragments arranged by size:
4552 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2416 2416
Size: 6097
PshAI GACnn'nnGTC
Cuts at: 1613 2820 1613
Size: 1207 4890
PsiI TTA'TAA
Cuts at: 3667 3667
Size: 6097
PstI C_TGCA'G
Cuts at: 830 2311 3449 830
Size: 1481 1138 3478
Fragments arranged by size:
3478 1481 1138
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6097
PvuII CAG'CTG
Cuts at: 2306 3368 2306
Size: 1062 5035
SacI G_AGCT'C
Cuts at: 2622 2622
Size: 6097
SalI G'TCGA_C
Cuts at: 1936 3439 1936
Size: 1503 4594
SanDI GG'GwC_CC
Cuts at: 2416 2416
Size: 6097
SapI GCTCTTCn'nnn_
Cuts at: 2232 2232
Size: 6097
SbfI CC_TGCA'GG
Cuts at: 3449 3449
Size: 6097
ScaI AGT'ACT
Cuts at: 4345 6057 4345
Size: 1712 4385
SfcI C'TryA_G
Cuts at: 826 1045 2307 2781 2877 3445 4580 5261 5452
Size: 219 1262 474 96 568 1135 681 191
Cuts at: 5452 826
Size: 1471
Fragments arranged by size:
1471 1262 1135 681 568 474 219 191
96
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6097
SmaI CCC'GGG
Cuts at: 1872 3424 1872
Size: 1552 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4201 5102 5364 820
Size: 197 926 2258 901 262 1553
Fragments arranged by size:
2258 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3888 493
Size: 3395 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6097
SphI G_CATG'C
Cuts at: 1132 3455 1132
Size: 2323 3774
SspI AAT'ATT
Cuts at: 5 52 4021 5
Size: 47 3969 2081
Fragments arranged by size:
3969 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6097
StyI C'CwwG_G
Cuts at: 513 1065 2280 3536 513
Size: 552 1215 1256 3074
Fragments arranged by size:
3074 1256 1215 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2577 4124 4141 4294 4479 4924 325
Size: 1255 997 1547 17 153 185 445 1498
Fragments arranged by size:
1547 1498 1255 997 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3116 4343 6055
Size: 274 80 33 51 1443 1139 1227 1712
Cuts at: 6055 96
Size: 138
Fragments arranged by size:
1712 1443 1227 1139 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6057 6089 6057
Size: 32 6065
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6097
XbaI T'CTAG_A
Cuts at: 3433 3433
Size: 6097
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6097
XmaI C'CCGG_G
Cuts at: 1870 3422 1870
Size: 1552 4545
XmnI GAAnn'nnTTC
Cuts at: 2636 4226 2636
Size: 1590 4507
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3905 276
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI
SrfI SwaI XcmI