Restriction map for
pFN21AB7372
(Circular) (Six-base) MAPSORT of: pFN21AB7372.seq Check: 6982 from: 1 to: 5590
pFN21AB7372 5590 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2619 3400 278
Size: 53 83 186 936 1083 781 2468
Fragments arranged by size:
2468 1083 936 781 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2745 2933 1937
Size: 808 188 4594
Fragments arranged by size:
4594 808 188
Acc65I G'GTAC_C
Cuts at: 2911 2911
Size: 5590
AclI AA'CG_TT
Cuts at: 1563 3717 4090 1563
Size: 2154 373 3063
Fragments arranged by size:
3063 2154 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3653 5037 5305 1632
Size: 2021 1384 268 1917
Fragments arranged by size:
2021 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5393
AflIII A'CryG_T
Cuts at: 1203 2643 2720 4489 5466 1203
Size: 1440 77 1769 977 1327
Fragments arranged by size:
1769 1440 1327 977 77
AgeI A'CCGG_T
Cuts at: 4421 4421
Size: 5590
AhdI GACnn_n'nnGTC
Cuts at: 4319 4319
Size: 5590
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5590
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5590
AlwNI CAG_nnn'CTG
Cuts at: 3333 4905 3333
Size: 1572 4018
ApaLI G'TGCA_C
Cuts at: 3647 4803 3647
Size: 1156 4434
ApoI r'AATT_y
Cuts at: 1493 2899 3125 3277 5386 1493
Size: 1406 226 152 2109 1697
Fragments arranged by size:
2109 1697 1406 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4144 160
Size: 872 3112 1606
Fragments arranged by size:
3112 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5590
AvaI C'yCGr_G
Cuts at: 1870 1943 2915 1870
Size: 73 972 4545
Fragments arranged by size:
4545 972 73
BamHI G'GATC_C
Cuts at: 2920 3287 2920
Size: 367 5223
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2133 2911 4366 618
Size: 325 217 627 346 778 1455 1842
Fragments arranged by size:
1842 1455 778 627 346 325 217
BanII G_rGCy'C
Cuts at: 1391 2909 1391
Size: 1518 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2662 928
Size: 971 763 3856
Fragments arranged by size:
3856 971 763
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3781 3815 1903
Size: 34 1844 34 3678
Fragments arranged by size:
3678 1844 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3482 4692 3482
Size: 1210 4380
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5590
BfrBI ATG'CAT
Cuts at: 2029 2059 2029
Size: 30 5560
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4201 136
Size: 107 122 71 631 605 2529 1525
Fragments arranged by size:
2529 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2604 5585 2604
Size: 2981 2609
BlpI GC'TnA_GC
Cuts at: 3007 3007
Size: 5590
Bme1580I G_kGCm'C
Cuts at: 1790 3651 4807 1790
Size: 1861 1156 2573
Fragments arranged by size:
2573 1861 1156
BmgBI CAC'GTC
Cuts at: 1318 2788 1318
Size: 1470 4120
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3321 4279 442
Size: 416 53 83 399 24 1904 958 1753
Fragments arranged by size:
1904 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5590
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4250 1239
Size: 3011 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2735 3715 4580 4878 2735
Size: 980 865 298 3447
Fragments arranged by size:
3447 980 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4253 882
Size: 617 2754 2219
Fragments arranged by size:
2754 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2646 3381 493
Size: 711 1442 735 2702
Fragments arranged by size:
2702 1442 735 711
BsaBI GATnn'nnATC
Cuts at: 2231 5584 2231
Size: 3353 2237
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2616 3397 3779 4414
Size: 53 83 186 936 1083 781 382 635
Cuts at: 4414 275
Size: 1451
Fragments arranged by size:
1451 1083 936 781 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4023 4421 4695 4842 5253 4023
Size: 398 274 147 411 4360
Fragments arranged by size:
4360 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2126 2156 1178
Size: 30 165 30 723 30 4612
Fragments arranged by size:
4612 723 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5590
BseYI C'CCAG_C
Cuts at: 2103 4793 5289 2103
Size: 2690 496 2404
Fragments arranged by size:
2690 2404 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2138 2189 2529 1163
Size: 573 402 51 340 4224
Fragments arranged by size:
4224 573 402 340 51
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3040 3801 4829 1540
Size: 275 186 1039 761 1028 2301
Fragments arranged by size:
2301 1039 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2019 3651 3736 4807 2019
Size: 1632 85 1071 2802
Fragments arranged by size:
2802 1632 1071 85
BsmI GAATG_Cn'
Cuts at: 3101 3194 3101
Size: 93 5497
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2019 2909 3651 3736 4807 1391
Size: 399 229 890 742 85 1071 2174
Fragments arranged by size:
2174 1071 890 742 399 229 85
BspEI T'CCGG_A
Cuts at: 5253 5253
Size: 5590
BspHI T'CATG_A
Cuts at: 3479 5209 3479
Size: 1730 3860
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2945 844
Size: 2101 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3477 1394
Size: 429 1654 3507
Fragments arranged by size:
3507 1654 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4085 4259 65
Size: 1183 2837 174 1396
Fragments arranged by size:
2837 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4234 4421 1700
Size: 110 2424 187 2869
Fragments arranged by size:
2869 2424 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5590
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5590
BssSI C'ACGA_G
Cuts at: 2675 3650 4662 2675
Size: 975 1012 3603
Fragments arranged by size:
3603 1012 975
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5590
BstBI TT'CG_AA
Cuts at: 4394 4394
Size: 5590
BstEII G'GTnAC_C
Cuts at: 1184 2155 1184
Size: 971 4619
BstYI r'GATC_y
Cuts at: 1972 2232 2604 2920 3287 3672 3689 5141 5227
Size: 260 372 316 367 385 17 1452 86
Cuts at: 5227 5239 5585 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 372 367 346 316 260
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2041 2433 2689 513
Size: 552 976 392 256 3414
Fragments arranged by size:
3414 976 552 392 256
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2068 487
Size: 683 56 842 4009
Fragments arranged by size:
4009 842 683 56
BtsI GCAGTG_nn'
Cuts at: 2126 3106 3900 3920 2126
Size: 980 794 20 3796
Fragments arranged by size:
3796 980 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5590
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2023 2058 2076 2111 2341 2376 529
Size: 35 1459 35 18 35 230 35 3743
Fragments arranged by size:
3743 1459 230 35 35 35 35 18
DraI TTT'AAA
Cuts at: 2894 2894
Size: 5590
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4597 809
Size: 507 3281 1802
Fragments arranged by size:
3281 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2044 3037 3926
Size: 54 1425 50 275 18 214 993 889
Cuts at: 3926 5555 8
Size: 1629 43
Fragments arranged by size:
1629 1425 993 889 275 214 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3037 1537
Size: 275 1225 4090
Fragments arranged by size:
4090 1225 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2462 2619 3520 1353
Size: 1109 157 901 3423
Fragments arranged by size:
3423 1109 901 157
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4177 4551 4697 387
Size: 1064 138 2588 374 146 1280
Fragments arranged by size:
2588 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3653 4250 5037 5305 1239
Size: 393 2021 597 787 268 1524
Fragments arranged by size:
2021 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2776 2776
Size: 5590
EcoRI G'AATT_C
Cuts at: 2899 3277 2899
Size: 378 5212
FspI TGC'GCA
Cuts at: 4096 4096
Size: 5590
HaeII r_GCGC'y
Cuts at: 1678 4737 1678
Size: 3059 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4231 4263 4305 4337 1373
Size: 32 135 32 2659 32 42 32 2626
Fragments arranged by size:
2659 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2934 3180 669
Size: 988 281 996 246 3079
Fragments arranged by size:
3079 996 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5590
HpaI GTT'AAC
Cuts at: 3180 3180
Size: 5590
KpnI G_GTAC'C
Cuts at: 2915 2915
Size: 5590
MfeI C'AATT_G
Cuts at: 3189 3189
Size: 5590
MluI A'CGCG_T
Cuts at: 5466 5466
Size: 5590
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4704 4888 1305
Size: 3399 184 2007
Fragments arranged by size:
3399 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2046 10
Size: 54 1425 343 214 3554
Fragments arranged by size:
3554 1425 343 214 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3548 3907 4066 518
Size: 648 2382 359 159 2042
Fragments arranged by size:
2382 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2107 3006 3683 4831 5076 1606
Size: 501 899 677 1148 245 2120
Fragments arranged by size:
2120 1148 899 677 501 245
NcoI C'CATG_G
Cuts at: 513 1065 2433 513
Size: 552 1368 3670
Fragments arranged by size:
3670 1368 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5590
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5590
NotI GC'GGCC_GC
Cuts at: 3037 3037
Size: 5590
NsiI A_TGCA'T
Cuts at: 2031 2061 2031
Size: 30 5560
NspI r_CATG'y
Cuts at: 1132 1229 2029 2033 2063 2557 2948 4493 1132
Size: 97 800 4 30 494 391 1545 2229
Fragments arranged by size:
2229 1545 800 494 391 97 30 4
PasI CC'CwG_GG
Cuts at: 1348 2772 1348
Size: 1424 4166
PciI A'CATG_T
Cuts at: 4489 4489
Size: 5590
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5590
PmeI GTTT'AAAC
Cuts at: 2894 2894
Size: 5590
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3659 3691 5172 5204 3659
Size: 32 1481 32 4045
Fragments arranged by size:
4045 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5590
PsiI TTA'TAA
Cuts at: 2316 3160 2316
Size: 844 4746
PstI C_TGCA'G
Cuts at: 830 2942 830
Size: 2112 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5590
PvuII CAG'CTG
Cuts at: 2107 2107
Size: 5590
SalI G'TCGA_C
Cuts at: 1936 2932 1936
Size: 996 4594
SbfI CC_TGCA'GG
Cuts at: 2942 2942
Size: 5590
ScaI AGT'ACT
Cuts at: 3838 5550 3838
Size: 1712 3878
SfcI C'TryA_G
Cuts at: 826 1045 2539 2938 4073 4754 4945 826
Size: 219 1494 399 1135 681 191 1471
Fragments arranged by size:
1494 1471 1135 681 399 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5590
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5590
SmaI CCC'GGG
Cuts at: 1872 2917 1872
Size: 1045 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2750 3694 4595 4857 820
Size: 197 926 807 944 901 262 1553
Fragments arranged by size:
1553 944 926 901 807 262 197
SnaBI TAC'GTA
Cuts at: 493 3381 493
Size: 2888 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5590
SphI G_CATG'C
Cuts at: 1132 2029 2033 2063 2557 2948 1132
Size: 897 4 30 494 391 3774
Fragments arranged by size:
3774 897 494 391 30 4
SspI AAT'ATT
Cuts at: 5 52 3514 5
Size: 47 3462 2081
Fragments arranged by size:
3462 2081 47
StuI AGG'CCT
Cuts at: 1517 2782 1517
Size: 1265 4325
StyI C'CwwG_G
Cuts at: 513 1065 2091 2303 2433 2860 3029 513
Size: 552 1026 212 130 427 169 3074
Fragments arranged by size:
3074 1026 552 427 212 169 130
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2151 3617 3634 3787 3972 4417 325
Size: 1255 571 1466 17 153 185 445 1498
Fragments arranged by size:
1498 1466 1255 571 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2290 3836 5548
Size: 274 80 33 51 1443 313 1546 1712
Cuts at: 5548 96
Size: 138
Fragments arranged by size:
1712 1546 1443 313 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2126 2158 5550 5582 2126
Size: 32 3392 32 2134
Fragments arranged by size:
3392 2134 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5590
XbaI T'CTAG_A
Cuts at: 2926 2926
Size: 5590
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5590
XmaI C'CCGG_G
Cuts at: 1870 2915 1870
Size: 1045 4545
XmnI GAAnn'nnTTC
Cuts at: 3719 3719
Size: 5590
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2617 3398 276
Size: 53 83 186 936 1083 781 2468
Fragments arranged by size:
2468 1083 936 781 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI