Restriction map for
pFN21AB7363
(Circular) (Six-base) MAPSORT of: pFN21AB7363.seq Check: 4727 from: 1 to: 5791
pFN21AB7363 5791 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3601 278
Size: 53 83 186 936 2065 2468
Fragments arranged by size:
2468 2065 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3134 1937
Size: 1197 4594
Acc65I G'GTAC_C
Cuts at: 3084 3112 3084
Size: 28 5763
AclI AA'CG_TT
Cuts at: 1563 3918 4291 1563
Size: 2355 373 3063
Fragments arranged by size:
3063 2355 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3854 5238 5506 1632
Size: 2222 1384 268 1917
Fragments arranged by size:
2222 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5594
AflIII A'CryG_T
Cuts at: 1203 4690 5667 1203
Size: 3487 977 1327
Fragments arranged by size:
3487 1327 977
AgeI A'CCGG_T
Cuts at: 4622 4622
Size: 5791
AhdI GACnn_n'nnGTC
Cuts at: 4520 4520
Size: 5791
AleI CACnn'nnGTG
Cuts at: 1166 2366 1166
Size: 1200 4591
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2666 1184
Size: 1482 4309
AlwNI CAG_nnn'CTG
Cuts at: 2846 3534 5106 2846
Size: 688 1572 3531
Fragments arranged by size:
3531 1572 688
ApaI G_GGCC'C
Cuts at: 3056 3056
Size: 5791
ApaLI G'TGCA_C
Cuts at: 3848 5004 3848
Size: 1156 4635
ApoI r'AATT_y
Cuts at: 1493 2508 2738 3100 3326 3478 5587 1493
Size: 1015 230 362 226 152 2109 1697
Fragments arranged by size:
2109 1697 1015 362 230 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4345 160
Size: 872 3313 1606
Fragments arranged by size:
3313 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5791
AvaI C'yCGr_G
Cuts at: 1870 1943 2592 2604 2764 2783 2983 3116 1870
Size: 73 649 12 160 19 200 133 4545
Fragments arranged by size:
4545 649 200 160 133 73 19 12
BamHI G'GATC_C
Cuts at: 3121 3488 3121
Size: 367 5424
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2448 3084 3112 4567 618
Size: 325 217 627 661 636 28 1455 1842
Fragments arranged by size:
1842 1455 661 636 627 325 217 28
BanII G_rGCy'C
Cuts at: 1391 2259 2588 2961 3056 3110 1391
Size: 868 329 373 95 54 4072
Fragments arranged by size:
4072 868 373 329 95 54
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4820
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3016 3050 3982 4016 1903
Size: 34 1079 34 932 34 3678
Fragments arranged by size:
3678 1079 932 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3683 4893 3683
Size: 1210 4581
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5791
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4402 136
Size: 107 122 71 631 605 2730 1525
Fragments arranged by size:
2730 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2334 5786 2334
Size: 3452 2339
BlpI GC'TnA_GC
Cuts at: 2556 2587 3208 2556
Size: 31 621 5139
Fragments arranged by size:
5139 621 31
Bme1580I G_kGCm'C
Cuts at: 1790 3056 3852 5008 1790
Size: 1266 796 1156 2573
Fragments arranged by size:
2573 1266 1156 796
BmgBI CAC'GTC
Cuts at: 1318 2377 1318
Size: 1059 4732
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2768 3522 4480
Size: 416 53 83 399 24 1351 754 958
Cuts at: 4480 442
Size: 1753
Fragments arranged by size:
1753 1351 958 754 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5791
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2278 2278
Size: 5791
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2877 4451 1239
Size: 1638 1574 2579
Fragments arranged by size:
2579 1638 1574
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3916 4781 5079 3916
Size: 865 298 4628
Fragments arranged by size:
4628 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2607 4454 882
Size: 617 1108 1847 2219
Fragments arranged by size:
2219 1847 1108 617
BsaAI yAC'GTr
Cuts at: 493 1204 3582 493
Size: 711 2378 2702
Fragments arranged by size:
2702 2378 711
BsaBI GATnn'nnATC
Cuts at: 2072 5785 2072
Size: 3713 2078
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2415 2628 3598 3980
Size: 53 83 186 936 882 213 970 382
Cuts at: 3980 4615 275
Size: 635 1451
Fragments arranged by size:
1451 970 936 882 635 382 213 186
83 53
BsaWI w'CCGG_w
Cuts at: 4224 4622 4896 5043 5454 4224
Size: 398 274 147 411 4561
Fragments arranged by size:
4561 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2486 2516 2558 2588 2869
Size: 30 165 30 1083 30 42 30 281
Cuts at: 2869 2899 1178
Size: 30 4070
Fragments arranged by size:
4070 1083 281 165 42 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5791
BseYI C'CCAG_C
Cuts at: 2022 2638 2653 2906 3023 4994 5490 2022
Size: 616 15 253 117 1971 496 2323
Fragments arranged by size:
2323 1971 616 496 253 117 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5218
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3241 4002 5030 1540
Size: 275 186 1240 761 1028 2301
Fragments arranged by size:
2301 1240 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2259 2305 2961 3852 3937 5008 2259
Size: 46 656 891 85 1071 3042
Fragments arranged by size:
3042 1071 891 656 85 46
BsmI GAATG_Cn'
Cuts at: 3302 3395 3302
Size: 93 5698
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2259 2305 2588 2961 3056 3110 3852
Size: 399 469 46 283 373 95 54 742
Cuts at: 3852 3937 5008 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 469 399 373 283 95
85 54 46
BspEI T'CCGG_A
Cuts at: 5454 5454
Size: 5791
BspHI T'CATG_A
Cuts at: 2518 3680 5410 2518
Size: 1162 1730 2899
Fragments arranged by size:
2899 1730 1162
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3146 844
Size: 2302 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2752 3678 1394
Size: 429 929 926 3507
Fragments arranged by size:
3507 929 926 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4286 4460 65
Size: 1183 3038 174 1396
Fragments arranged by size:
3038 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2445 3029 4435 4622 1700
Size: 110 635 584 1406 187 2869
Fragments arranged by size:
2869 1406 635 584 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5791
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5791
BssSI C'ACGA_G
Cuts at: 2252 3851 4863 2252
Size: 1599 1012 3180
Fragments arranged by size:
3180 1599 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5791
BstBI TT'CG_AA
Cuts at: 4595 4595
Size: 5791
BstEII G'GTnAC_C
Cuts at: 1184 2657 1184
Size: 1473 4318
BstXI CCAn_nnnn'nTGG
Cuts at: 3047 3047
Size: 5791
BstYI r'GATC_y
Cuts at: 1972 2180 2334 3121 3488 3873 3890 5342 5428
Size: 208 154 787 367 385 17 1452 86
Cuts at: 5428 5440 5786 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 787 385 367 346 208 154
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2280 2280
Size: 5791
BtgI C'CryG_G
Cuts at: 513 1065 2004 2364 2949 513
Size: 552 939 360 585 3355
Fragments arranged by size:
3355 939 585 552 360
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5052
Fragments arranged by size:
5052 683 56
BtsI GCAGTG_nn'
Cuts at: 2805 3307 4101 4121 2805
Size: 502 794 20 4475
Fragments arranged by size:
4475 794 502 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5791
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5756
DraI TTT'AAA
Cuts at: 3095 3095
Size: 5791
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2987 4798 809
Size: 507 1671 1811 1802
Fragments arranged by size:
1811 1802 1671 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2125 2952 2970
Size: 54 1425 50 275 18 295 827 18
Cuts at: 2970 3037 3238 4127 5756 8
Size: 67 201 889 1629 43
Fragments arranged by size:
1629 1425 889 827 295 275 201 67
54 50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 3238 1537
Size: 275 1426 4090
Fragments arranged by size:
4090 1426 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3721 1353
Size: 2368 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4378 4752 4898 387
Size: 1064 138 2789 374 146 1280
Fragments arranged by size:
2789 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2257 2959 2257
Size: 702 5089
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2877 3854 4451 5238 5506 1239
Size: 393 1245 977 597 787 268 1524
Fragments arranged by size:
1524 1245 977 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2164 3052 3053 2164
Size: 888 1 4902
Fragments arranged by size:
4902 888 1
EcoRI G'AATT_C
Cuts at: 2738 3100 3478 2738
Size: 362 378 5051
Fragments arranged by size:
5051 378 362
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2305 2353 2305
Size: 48 5743
FspI TGC'GCA
Cuts at: 4297 4297
Size: 5791
HaeII r_GCGC'y
Cuts at: 1678 2772 3009 4938 1678
Size: 1094 237 1929 2531
Fragments arranged by size:
2531 1929 1094 237
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2305 2337 2558 2590 2851
Size: 32 135 32 733 32 221 32 261
Cuts at: 2851 2869 2883 2901 4432 4464 4506 4538 1373
Size: 18 14 18 1531 32 42 32 2626
Fragments arranged by size:
2626 1531 733 261 221 135 42 32
32 32 32 32 32 18 18 14
HincII GTy'rAC
Cuts at: 669 1657 1938 3135 3381 669
Size: 988 281 1197 246 3079
Fragments arranged by size:
3079 1197 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5791
HpaI GTT'AAC
Cuts at: 3381 3381
Size: 5791
KpnI G_GTAC'C
Cuts at: 3088 3116 3088
Size: 28 5763
MfeI C'AATT_G
Cuts at: 2271 3390 2271
Size: 1119 4672
MluI A'CGCG_T
Cuts at: 5667 5667
Size: 5791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4905 5089 1305
Size: 3600 184 2007
Fragments arranged by size:
3600 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3969
Fragments arranged by size:
3969 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2207 2366 2423 2517 2648 3749 4108
Size: 648 1041 159 57 94 131 1101 359
Cuts at: 4108 4267 518
Size: 159 2042
Fragments arranged by size:
2042 1101 1041 648 359 159 159 131
94 57
MspA1I CmG'CkG
Cuts at: 1606 2834 2843 3207 3884 5032 5277 1606
Size: 1228 9 364 677 1148 245 2120
Fragments arranged by size:
2120 1228 1148 677 364 245 9
NcoI C'CATG_G
Cuts at: 513 1065 2004 2949 513
Size: 552 939 945 3355
Fragments arranged by size:
3355 945 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5791
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5791
NotI GC'GGCC_GC
Cuts at: 3238 3238
Size: 5791
NspI r_CATG'y
Cuts at: 1132 1229 2653 3149 4694 1132
Size: 97 1424 496 1545 2229
Fragments arranged by size:
2229 1545 1424 496 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5791
PciI A'CATG_T
Cuts at: 4690 4690
Size: 5791
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5791
PmeI GTTT'AAAC
Cuts at: 3095 3095
Size: 5791
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3860 3892 5373 5405 3860
Size: 32 1481 32 4246
Fragments arranged by size:
4246 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2164 2164
Size: 5791
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5791
PsiI TTA'TAA
Cuts at: 3361 3361
Size: 5791
PspOMI G'GGCC_C
Cuts at: 3052 3052
Size: 5791
PstI C_TGCA'G
Cuts at: 830 3143 830
Size: 2313 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5791
SacI G_AGCT'C
Cuts at: 2259 2961 2259
Size: 702 5089
SalI G'TCGA_C
Cuts at: 1936 3133 1936
Size: 1197 4594
SanDI GG'GwC_CC
Cuts at: 2164 2164
Size: 5791
SbfI CC_TGCA'GG
Cuts at: 3143 3143
Size: 5791
ScaI AGT'ACT
Cuts at: 4039 5751 4039
Size: 1712 4079
SfcI C'TryA_G
Cuts at: 826 1045 3139 4274 4955 5146 826
Size: 219 2094 1135 681 191 1471
Fragments arranged by size:
2094 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5791
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5791
SmaI CCC'GGG
Cuts at: 1872 2594 3118 1872
Size: 722 524 4545
Fragments arranged by size:
4545 722 524
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2604 3895 4796 5058 820
Size: 197 926 661 1291 901 262 1553
Fragments arranged by size:
1553 1291 926 901 661 262 197
SnaBI TAC'GTA
Cuts at: 493 3582 493
Size: 3089 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5791
SphI G_CATG'C
Cuts at: 1132 2653 3149 1132
Size: 1521 496 3774
Fragments arranged by size:
3774 1521 496
SspI AAT'ATT
Cuts at: 5 52 2387 3715 5
Size: 47 2335 1328 2081
Fragments arranged by size:
2335 2081 1328 47
StuI AGG'CCT
Cuts at: 1517 2706 1517
Size: 1189 4602
StyI C'CwwG_G
Cuts at: 513 1065 2004 2949 3230 513
Size: 552 939 945 281 3074
Fragments arranged by size:
3074 945 939 552 281
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3818 3835 3988 4173 4618 325
Size: 1255 2238 17 153 185 445 1498
Fragments arranged by size:
2238 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4037 5749 96
Size: 274 80 33 51 1443 2060 1712 138
Fragments arranged by size:
2060 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2486 2518 5751 5783 2486
Size: 32 3233 32 2494
Fragments arranged by size:
3233 2494 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5791
XbaI T'CTAG_A
Cuts at: 3127 3127
Size: 5791
XhoI C'TCGA_G
Cuts at: 1943 2604 1943
Size: 661 5130
XmaI C'CCGG_G
Cuts at: 1870 2592 3116 1870
Size: 722 524 4545
Fragments arranged by size:
4545 722 524
XmnI GAAnn'nnTTC
Cuts at: 3920 3920
Size: 5791
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3599 276
Size: 53 83 186 936 2065 2468
Fragments arranged by size:
2468 2065 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SacI SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspXI PsrI PvuII RsrII SacII
SapI SexAI SfiI SfoI SrfI SwaI XcmI