Restriction map for
pFN21AB7358
(Circular) (Six-base) MAPSORT of: pFN21AB7358.seq Check: 2429 from: 1 to: 5962
pFN21AB7358 5962 bp
With 182 enzymes: SgfI *
March 28, 2012 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3772 278
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3305 1937
Size: 1368 4594
Acc65I G'GTAC_C
Cuts at: 2082 3283 2082
Size: 1201 4761
AclI AA'CG_TT
Cuts at: 1563 4089 4462 1563
Size: 2526 373 3063
Fragments arranged by size:
3063 2526 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2610 2852 2886 4025 5409 5677 1632
Size: 978 242 34 1139 1384 268 1917
Fragments arranged by size:
1917 1384 1139 978 268 242 34
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5765
AflIII A'CryG_T
Cuts at: 1203 4861 5838 1203
Size: 3658 977 1327
Fragments arranged by size:
3658 1327 977
AgeI A'CCGG_T
Cuts at: 4793 4793
Size: 5962
AhdI GACnn_n'nnGTC
Cuts at: 4691 4691
Size: 5962
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5962
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5962
AlwNI CAG_nnn'CTG
Cuts at: 3705 5277 3705
Size: 1572 4390
ApaLI G'TGCA_C
Cuts at: 4019 5175 4019
Size: 1156 4806
ApoI r'AATT_y
Cuts at: 1493 3271 3497 3649 5758 1493
Size: 1778 226 152 2109 1697
Fragments arranged by size:
2109 1778 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4516 160
Size: 872 3484 1606
Fragments arranged by size:
3484 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
AvaI C'yCGr_G
Cuts at: 1870 1943 3287 1870
Size: 73 1344 4545
Fragments arranged by size:
4545 1344 73
BamHI G'GATC_C
Cuts at: 3292 3659 3292
Size: 367 5595
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2082 3283 4738 618
Size: 325 217 627 295 1201 1455 1842
Fragments arranged by size:
1842 1455 1201 627 325 295 217
BanII G_rGCy'C
Cuts at: 1391 3281 1391
Size: 1890 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3032 928
Size: 971 1133 3858
Fragments arranged by size:
3858 1133 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4153 4187 1903
Size: 34 50 34 2132 34 3678
Fragments arranged by size:
3678 2132 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2459 3854 5064 2459
Size: 1395 1210 3357
Fragments arranged by size:
3357 1395 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5962
BfrBI ATG'CAT
Cuts at: 2367 2367
Size: 5962
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2151 4573
Size: 107 122 71 631 605 337 142 2422
Cuts at: 4573 136
Size: 1525
Fragments arranged by size:
2422 1525 631 605 337 142 122 107
71
BglII A'GATC_T
Cuts at: 5957 5957
Size: 5962
BlpI GC'TnA_GC
Cuts at: 2762 3379 2762
Size: 617 5345
Bme1580I G_kGCm'C
Cuts at: 1790 4023 5179 1790
Size: 2233 1156 2573
Fragments arranged by size:
2573 2233 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5962
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3693 4651 442
Size: 416 53 83 399 24 2276 958 1753
Fragments arranged by size:
2276 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2502 1056
Size: 1446 4516
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2020 2128 2692 3045 4622 1239
Size: 781 108 564 353 1577 2579
Fragments arranged by size:
2579 1577 781 564 353 108
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2862 4087 4952 5250 2862
Size: 1225 865 298 3574
Fragments arranged by size:
3574 1225 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4625 882
Size: 617 3126 2219
Fragments arranged by size:
3126 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3753 493
Size: 711 2549 2702
Fragments arranged by size:
2702 2549 711
BsaBI GATnn'nnATC
Cuts at: 2180 3146 5956 2180
Size: 966 2810 2186
Fragments arranged by size:
2810 2186 966
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3769 4151 4786 275
Size: 53 83 186 936 2236 382 635 1451
Fragments arranged by size:
2236 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4395 4793 5067 5214 5625 4395
Size: 398 274 147 411 4732
Fragments arranged by size:
4732 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2018 2048 1178
Size: 30 165 30 615 30 5092
Fragments arranged by size:
5092 615 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3100 1186
Size: 1914 4048
BseYI C'CCAG_C
Cuts at: 2431 3070 3098 5165 5661 2431
Size: 639 28 2067 496 2732
Fragments arranged by size:
2732 2067 639 496 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2262 1163
Size: 573 526 4863
Fragments arranged by size:
4863 573 526
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3412 4173 5201 1540
Size: 275 186 1411 761 1028 2301
Fragments arranged by size:
2301 1411 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4023 4108 5179 4023
Size: 85 1071 4806
Fragments arranged by size:
4806 1071 85
BsmI GAATG_Cn'
Cuts at: 3473 3566 3473
Size: 93 5869
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3281 4023 4108 5179 1391
Size: 399 1491 742 85 1071 2174
Fragments arranged by size:
2174 1491 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5625 5625
Size: 5962
BspHI T'CATG_A
Cuts at: 2545 3851 5581 2545
Size: 1306 1730 2926
Fragments arranged by size:
2926 1730 1306
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2094 3317 844
Size: 1250 1223 3489
Fragments arranged by size:
3489 1250 1223
BsrBI CCG'CTC
Cuts at: 1394 1823 3849 1394
Size: 429 2026 3507
Fragments arranged by size:
3507 2026 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2766 4457 4631 65
Size: 1183 1518 1691 174 1396
Fragments arranged by size:
1691 1518 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4606 4793 1700
Size: 110 2796 187 2869
Fragments arranged by size:
2869 2796 187 110
BsrGI T'GTAC_A
Cuts at: 96 3199 96
Size: 3103 2859
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5962
BssSI C'ACGA_G
Cuts at: 2567 4022 5034 2567
Size: 1455 1012 3495
Fragments arranged by size:
3495 1455 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5962
BstBI TT'CG_AA
Cuts at: 4766 4766
Size: 5962
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5962
BstYI r'GATC_y
Cuts at: 1972 2317 3292 3659 4044 4061 5513 5599 5611
Size: 345 975 367 385 17 1452 86 12
Cuts at: 5611 5957 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 975 385 367 346 345 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5410
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5223
Fragments arranged by size:
5223 683 56
BtsI GCAGTG_nn'
Cuts at: 3478 4272 4292 3478
Size: 794 20 5148
Fragments arranged by size:
5148 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5962
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2376 2411 529
Size: 35 1812 35 4080
Fragments arranged by size:
4080 1812 35 35
DraI TTT'AAA
Cuts at: 3266 3266
Size: 5962
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4969 809
Size: 507 3653 1802
Fragments arranged by size:
3653 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2400 2481 3409
Size: 54 1425 50 275 18 570 81 928
Cuts at: 3409 4298 5927 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 928 889 570 275 81 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3409 1537
Size: 275 1597 4090
Fragments arranged by size:
4090 1597 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2586 2905 2931 3892 1353
Size: 1233 319 26 961 3423
Fragments arranged by size:
3423 1233 961 319 26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4549 4923 5069 387
Size: 1064 138 2960 374 146 1280
Fragments arranged by size:
2960 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2020 2128 2610 2692 2852 2886 3045
Size: 393 388 108 482 82 160 34 159
Cuts at: 3045 4025 4622 5409 5677 1239
Size: 980 597 787 268 1524
Fragments arranged by size:
1524 980 787 597 482 393 388 268
160 159 108 82 34
EcoRI G'AATT_C
Cuts at: 3271 3649 3271
Size: 378 5584
FspI TGC'GCA
Cuts at: 4468 4468
Size: 5962
HaeII r_GCGC'y
Cuts at: 1678 5109 1678
Size: 3431 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2018 2050 2224 2256 2585
Size: 32 135 32 446 32 174 32 329
Cuts at: 2585 2617 2701 2733 4603 4635 4677 4709 1373
Size: 32 84 32 1870 32 42 32 2626
Fragments arranged by size:
2626 1870 446 329 174 135 84 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3306 3552 669
Size: 988 281 1368 246 3079
Fragments arranged by size:
3079 1368 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5962
HpaI GTT'AAC
Cuts at: 3552 3552
Size: 5962
KpnI G_GTAC'C
Cuts at: 2086 3287 2086
Size: 1201 4761
MfeI C'AATT_G
Cuts at: 3561 3561
Size: 5962
MluI A'CGCG_T
Cuts at: 5838 5838
Size: 5962
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2913 3017 5076 5260 1305
Size: 1608 104 2059 184 2007
Fragments arranged by size:
2059 2007 1608 184 104
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2402 2483 10
Size: 54 1425 343 570 81 3489
Fragments arranged by size:
3489 1425 570 343 81 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2048 2366 2550 2753 3920 4279 4438
Size: 648 882 318 184 203 1167 359 159
Cuts at: 4438 518
Size: 2042
Fragments arranged by size:
2042 1167 882 648 359 318 203 184
159
MspA1I CmG'CkG
Cuts at: 1606 2015 2582 3023 3378 4055 5203 5448 1606
Size: 409 567 441 355 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 567 441 409 355 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5410
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5962
NheI G'CTAG_C
Cuts at: 1052 2498 1052
Size: 1446 4516
NotI GC'GGCC_GC
Cuts at: 3409 3409
Size: 5962
NsiI A_TGCA'T
Cuts at: 2369 2369
Size: 5962
NspI r_CATG'y
Cuts at: 1132 1229 2367 3320 4865 1132
Size: 97 1138 953 1545 2229
Fragments arranged by size:
2229 1545 1138 953 97
PasI CC'CwG_GG
Cuts at: 1348 3222 1348
Size: 1874 4088
PciI A'CATG_T
Cuts at: 4861 4861
Size: 5962
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5962
PmeI GTTT'AAAC
Cuts at: 3266 3266
Size: 5962
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2695 2727 4031 4063 5544 5576 2695
Size: 32 1304 32 1481 32 3081
Fragments arranged by size:
3081 1481 1304 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5962
PsiI TTA'TAA
Cuts at: 3532 3532
Size: 5962
PstI C_TGCA'G
Cuts at: 830 2842 3314 830
Size: 2012 472 3478
Fragments arranged by size:
3478 2012 472
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5962
PvuII CAG'CTG
Cuts at: 2015 2582 3023 2015
Size: 567 441 4954
Fragments arranged by size:
4954 567 441
SalI G'TCGA_C
Cuts at: 1936 3304 1936
Size: 1368 4594
SbfI CC_TGCA'GG
Cuts at: 3314 3314
Size: 5962
ScaI AGT'ACT
Cuts at: 4210 5922 4210
Size: 1712 4250
SfcI C'TryA_G
Cuts at: 826 1045 2838 3310 4445 5126 5317 826
Size: 219 1793 472 1135 681 191 1471
Fragments arranged by size:
1793 1471 1135 681 472 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5962
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5962
SmaI CCC'GGG
Cuts at: 1872 3289 1872
Size: 1417 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2877 4066 4967 5229 820
Size: 197 926 934 1189 901 262 1553
Fragments arranged by size:
1553 1189 934 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3753 493
Size: 3260 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5962
SphI G_CATG'C
Cuts at: 1132 3320 1132
Size: 2188 3774
SspI AAT'ATT
Cuts at: 5 52 3886 5
Size: 47 3834 2081
Fragments arranged by size:
3834 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5962
StyI C'CwwG_G
Cuts at: 513 1065 3401 513
Size: 552 2336 3074
Fragments arranged by size:
3074 2336 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3989 4006 4159 4344 4789 325
Size: 1255 2409 17 153 185 445 1498
Fragments arranged by size:
2409 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3199 4208 5920
Size: 274 80 33 51 1443 1222 1009 1712
Cuts at: 5920 96
Size: 138
Fragments arranged by size:
1712 1443 1222 1009 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5922 5954 5922
Size: 32 5930
Tth111I GACn'n_nGTC
Cuts at: 1316 3188 1316
Size: 1872 4090
XbaI T'CTAG_A
Cuts at: 3298 3298
Size: 5962
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2491 2491
Size: 5962
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5962
XmaI C'CCGG_G
Cuts at: 1870 3287 1870
Size: 1417 4545
XmnI GAAnn'nnTTC
Cuts at: 4091 4091
Size: 5962
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3770 276
Size: 53 83 186 936 2236 2468
Fragments arranged by size:
2468 2236 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI