Restriction map for
pFN21AB7349
(Circular) (Six-base) MAPSORT of: pFN21AB7349.seq Check: 9559 from: 1 to: 7753
pFN21AB7349 7753 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2317 2844 4576 4588 2317
Size: 527 1732 12 5482
Fragments arranged by size:
5482 1732 527 12
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2025 5563 278
Size: 53 83 186 936 489 3538 2468
Fragments arranged by size:
3538 2468 936 489 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5096 1937
Size: 3159 4594
Acc65I G'GTAC_C
Cuts at: 5074 5074
Size: 7753
AclI AA'CG_TT
Cuts at: 1563 5880 6253 1563
Size: 4317 373 3063
Fragments arranged by size:
4317 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2462 2561 2648 2660 3601 3688 3741 3896
Size: 830 99 87 12 941 87 53 155
Cuts at: 3896 4274 4289 4406 4679 5816 7200 7468 1632
Size: 378 15 117 273 1137 1384 268 1917
Fragments arranged by size:
1917 1384 1137 941 830 378 273 268
155 117 99 87 87 53 15 12
AfeI AGC'GCT
Cuts at: 3098 4055 4489 3098
Size: 957 434 6362
Fragments arranged by size:
6362 957 434
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7556
AflIII A'CryG_T
Cuts at: 1203 2040 6652 7629 1203
Size: 837 4612 977 1327
Fragments arranged by size:
4612 1327 977 837
AgeI A'CCGG_T
Cuts at: 6584 6584
Size: 7753
AhdI GACnn_n'nnGTC
Cuts at: 6482 6482
Size: 7753
AleI CACnn'nnGTG
Cuts at: 1166 4965 1166
Size: 3799 3954
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4091 4332 4656 4877 1184
Size: 2907 241 324 221 4060
Fragments arranged by size:
4060 2907 324 241 221
AlwNI CAG_nnn'CTG
Cuts at: 2059 2071 2332 3460 3928 5005 5496 7068 2059
Size: 12 261 1128 468 1077 491 1572 2744
Fragments arranged by size:
2744 1572 1128 1077 491 468 261 12
ApaI G_GGCC'C
Cuts at: 3401 4422 3401
Size: 1021 6732
ApaLI G'TGCA_C
Cuts at: 2633 5810 6966 2633
Size: 3177 1156 3420
Fragments arranged by size:
3420 3177 1156
ApoI r'AATT_y
Cuts at: 1493 3956 5062 5288 5440 7549 1493
Size: 2463 1106 226 152 2109 1697
Fragments arranged by size:
2463 2109 1697 1106 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6307 160
Size: 872 5275 1606
Fragments arranged by size:
5275 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7753
AvaI C'yCGr_G
Cuts at: 1870 1943 2240 2888 3494 4100 4848 5078 1870
Size: 73 297 648 606 606 748 230 4545
Fragments arranged by size:
4545 748 648 606 606 297 230 73
BamHI G'GATC_C
Cuts at: 3645 4906 5083 5450 3645
Size: 1261 177 367 5948
Fragments arranged by size:
5948 1261 367 177
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4967 5074 6529 618
Size: 325 217 627 3180 107 1455 1842
Fragments arranged by size:
3180 1842 1455 627 325 217 107
BanII G_rGCy'C
Cuts at: 1391 2241 2889 3039 3401 3551 3561 3642 4359
Size: 850 648 150 362 150 10 81 717
Cuts at: 4359 4422 4895 4941 5072 1391
Size: 63 473 46 131 4072
Fragments arranged by size:
4072 850 717 648 473 362 150 150
131 81 63 46 10
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2417 3729 4394 928
Size: 971 518 1312 665 4287
Fragments arranged by size:
4287 1312 971 665 518
BbvCI CC'TCA_GC
Cuts at: 3774 3774
Size: 7753
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3191 3225 5944 5978 1903
Size: 34 50 34 1170 34 2719 34 3678
Fragments arranged by size:
3678 2719 1170 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5645 6855 5645
Size: 1210 6543
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7753
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3236 3795 6364
Size: 107 122 71 631 605 1564 559 2569
Cuts at: 6364 136
Size: 1525
Fragments arranged by size:
2569 1564 1525 631 605 559 122 107
71
BglII A'GATC_T
Cuts at: 7748 7748
Size: 7753
BlpI GC'TnA_GC
Cuts at: 2322 2669 2984 3038 4158 5170 2322
Size: 347 315 54 1120 1012 4905
Fragments arranged by size:
4905 1120 1012 347 315 54
Bme1580I G_kGCm'C
Cuts at: 1790 2637 3401 4422 4972 5814 6970 1790
Size: 847 764 1021 550 842 1156 2573
Fragments arranged by size:
2573 1156 1021 847 842 764 550
BmgBI CAC'GTC
Cuts at: 1318 2041 2953 1318
Size: 723 912 6118
Fragments arranged by size:
6118 912 723
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3163 5484 6442
Size: 416 53 83 399 24 1746 2321 958
Cuts at: 6442 442
Size: 1753
Fragments arranged by size:
2321 1753 1746 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7753
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3544 3829 3544
Size: 285 7468
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2030 2207 2291 2366 2711 3077 3158 3920
Size: 791 177 84 75 345 366 81 762
Cuts at: 3920 3968 3982 4555 4694 6413 1239
Size: 48 14 573 139 1719 2579
Fragments arranged by size:
2579 1719 791 762 573 366 345 177
139 84 81 75 48 14
Bpu10I CC'TnA_GC
Cuts at: 3440 3774 4037 4622 3440
Size: 334 263 585 6571
Fragments arranged by size:
6571 585 334 263
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4034 4343 5878 6743 7041 4034
Size: 309 1535 865 298 4746
Fragments arranged by size:
4746 1535 865 309 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2209 2612 6416 882
Size: 617 710 403 3804 2219
Fragments arranged by size:
3804 2219 710 617 403
BsaAI yAC'GTr
Cuts at: 493 1204 5544 493
Size: 711 4340 2702
Fragments arranged by size:
4340 2702 711
BsaBI GATnn'nnATC
Cuts at: 7747 7747
Size: 7753
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2022 3147 5560 5942
Size: 53 83 186 936 489 1125 2413 382
Cuts at: 5942 6577 275
Size: 635 1451
Fragments arranged by size:
2413 1451 1125 936 635 489 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 6186 6584 6858 7005 7416 6186
Size: 398 274 147 411 6523
Fragments arranged by size:
6523 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2027 2057 3095 3125 1178
Size: 30 165 30 624 30 1038 30 5806
Fragments arranged by size:
5806 1038 624 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2048 2288 2291 2483 2546 2603 2666 2866
Size: 862 240 3 192 63 57 63 200
Cuts at: 2866 2867 3017 3071 3854 4160 4469 5006 1186
Size: 1 150 54 783 306 309 537 3933
Fragments arranged by size:
3933 862 783 537 309 306 240 200
192 150 63 63 57 54 3 1
BseYI C'CCAG_C
Cuts at: 2788 3692 3996 4079 5034 6956 7452 2788
Size: 904 304 83 955 1922 496 3089
Fragments arranged by size:
3089 1922 955 904 496 304 83
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2441 2454 4007 4165 1163
Size: 573 705 13 1553 158 4751
Fragments arranged by size:
4751 1553 705 573 158 13
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2354 3015 5203 5964 6992 1540
Size: 275 186 353 661 2188 761 1028 2301
Fragments arranged by size:
2301 2188 1028 761 661 353 275 186
BsiHKAI G_wGCw'C
Cuts at: 2241 2637 2889 2943 2985 3039 3081 3503 4359
Size: 396 252 54 42 54 42 422 856
Cuts at: 4359 4895 5814 5899 6970 2241
Size: 536 919 85 1071 3024
Fragments arranged by size:
3024 1071 919 856 536 422 396 252
85 54 54 42 42
BsmI GAATG_Cn'
Cuts at: 2362 3469 4162 5264 5357 2362
Size: 1107 693 1102 93 4758
Fragments arranged by size:
4758 1107 1102 693 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3134 3134
Size: 7753
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2241 2637 2889 2943 2985 3039 3081
Size: 399 451 396 252 54 42 54 42
Cuts at: 3081 3401 3503 3551 3561 3642 4359 4422 4895
Size: 320 102 48 10 81 717 63 473
Cuts at: 4895 4941 4972 5072 5814 5899 6970 1391
Size: 46 31 100 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 717 473 451 399 396
320 252 102 100 85 81 63 54
54 48 46 42 42 31 10
BspEI T'CCGG_A
Cuts at: 7416 7416
Size: 7753
BspHI T'CATG_A
Cuts at: 4029 5642 7372 4029
Size: 1613 1730 4410
Fragments arranged by size:
4410 1730 1613
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2099 2317 2673 2844 3633 4576 4588 5108
Size: 1255 218 356 171 789 943 12 520
Cuts at: 5108 844
Size: 3489
Fragments arranged by size:
3489 1255 943 789 520 356 218 171
12
BsrBI CCG'CTC
Cuts at: 1394 1823 2388 2550 2934 3823 4348 4411 4564
Size: 429 565 162 384 889 525 63 153
Cuts at: 4564 5640 1394
Size: 1076 3507
Fragments arranged by size:
3507 1076 889 565 525 429 384 162
153 63
BsrDI GCAATG_nn'
Cuts at: 65 1248 6248 6422 65
Size: 1183 5000 174 1396
Fragments arranged by size:
5000 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2109 2153 6397 6584 1700
Size: 110 299 44 4244 187 2869
Fragments arranged by size:
4244 2869 299 187 110 44
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7753
BssHII G'CGCG_C
Cuts at: 1925 2574 1925
Size: 649 7104
BssSI C'ACGA_G
Cuts at: 2942 3200 5813 6825 2942
Size: 258 2613 1012 3870
Fragments arranged by size:
3870 2613 1012 258
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2059 2091 2332 3766 794
Size: 1265 32 241 1434 4781
Fragments arranged by size:
4781 1434 1265 241 32
BstBI TT'CG_AA
Cuts at: 6557 6557
Size: 7753
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7753
BstYI r'GATC_y
Cuts at: 1972 3645 4716 4906 5083 5450 5835 5852 7304
Size: 1673 1071 190 177 367 385 17 1452
Cuts at: 7304 7390 7402 7748 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1673 1452 1071 385 367 346 190
177 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2763 3902 2763
Size: 1139 6614
BtgI C'CryG_G
Cuts at: 513 1065 2179 4288 513
Size: 552 1114 2109 3978
Fragments arranged by size:
3978 2109 1114 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7014
Fragments arranged by size:
7014 683 56
BtsI GCAGTG_nn'
Cuts at: 3533 4714 5269 6063 6083 3533
Size: 1181 555 794 20 5203
Fragments arranged by size:
5203 1181 794 555 20
ClaI AT'CG_AT
Cuts at: 1554 3291 1554
Size: 1737 6016
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7718
DraI TTT'AAA
Cuts at: 4767 5057 4767
Size: 290 7463
DraIII CAC_nnn'GTG
Cuts at: 4709 4709
Size: 7753
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3202 4652 6760 809
Size: 507 1886 1450 2108 1802
Fragments arranged by size:
2108 1886 1802 1450 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2155 2595 2922
Size: 54 1425 50 275 18 325 440 327
Cuts at: 2922 3012 4233 5200 6089 7718 8
Size: 90 1221 967 889 1629 43
Fragments arranged by size:
1629 1425 1221 967 889 440 327 325
275 90 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3012 5200 1537
Size: 275 1200 2188 4090
Fragments arranged by size:
4090 2188 1200 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2280 2844 4031 4137 5683 1353
Size: 927 564 1187 106 1546 3423
Fragments arranged by size:
3423 1546 1187 927 564 106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2811 4854 6340 6714 6860 387
Size: 1064 138 1222 2043 1486 374 146 1280
Fragments arranged by size:
2043 1486 1280 1222 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2239 2887 3037 4357 4893 2239
Size: 648 150 1320 536 5099
Fragments arranged by size:
5099 1320 648 536 150
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2030 2207 2291 2366 2462 2561 2648
Size: 393 398 177 84 75 96 99 87
Cuts at: 2648 2660 2711 3077 3158 3601 3688 3741 3896
Size: 12 51 366 81 443 87 53 155
Cuts at: 3896 3920 3968 3982 4274 4289 4406 4555 4679
Size: 24 48 14 292 15 117 149 124
Cuts at: 4679 4694 5816 6413 7200 7468 1239
Size: 15 1122 597 787 268 1524
Fragments arranged by size:
1524 1122 787 597 443 398 393 366
292 268 177 155 149 124 117 99
96 87 87 84 81 75 53 51
48 24 15 15 14 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2396 2396
Size: 7753
EcoO109I rG'GnC_Cy
Cuts at: 2563 2759 3379 3397 3398 3622 4418 4654 4984
Size: 196 620 18 1 224 796 236 330
Cuts at: 4984 2563
Size: 5332
Fragments arranged by size:
5332 796 620 330 236 224 196 18
1
EcoRI G'AATT_C
Cuts at: 5062 5440 5062
Size: 378 7375
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4030 4766 5026 4030
Size: 736 260 6757
Fragments arranged by size:
6757 736 260
FspI TGC'GCA
Cuts at: 2117 6259 2117
Size: 4142 3611
HaeII r_GCGC'y
Cuts at: 1678 2131 2875 2960 3100 3899 4057 4491 6900
Size: 453 744 85 140 799 158 434 2409
Cuts at: 6900 1678
Size: 2531
Fragments arranged by size:
2531 2409 799 744 453 434 158 140
85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3095 3127 3578 3610 3662
Size: 32 135 32 1523 32 451 32 52
Cuts at: 3662 3694 4141 4173 6394 6426 6468 6500 1373
Size: 32 447 32 2221 32 42 32 2626
Fragments arranged by size:
2626 2221 1523 451 447 135 52 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5097 5343 669
Size: 988 281 3159 246 3079
Fragments arranged by size:
3159 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2501 748
Size: 1753 6000
HpaI GTT'AAC
Cuts at: 5343 5343
Size: 7753
KpnI G_GTAC'C
Cuts at: 5078 5078
Size: 7753
MfeI C'AATT_G
Cuts at: 5352 5352
Size: 7753
MluI A'CGCG_T
Cuts at: 7629 7629
Size: 7753
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2943 3111 3241 6867 7051 1305
Size: 1638 168 130 3626 184 2007
Fragments arranged by size:
3626 2007 1638 184 168 130
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2924 10
Size: 54 1425 343 1092 4839
Fragments arranged by size:
4839 1425 1092 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2219 2745 3431 3983 4244 4965 5711
Size: 648 1053 526 686 552 261 721 746
Cuts at: 5711 6070 6229 518
Size: 359 159 2042
Fragments arranged by size:
2042 1053 746 721 686 648 552 526
359 261 159
MspA1I CmG'CkG
Cuts at: 1606 2050 2071 2299 3042 3763 3874 3940 4194
Size: 444 21 228 743 721 111 66 254
Cuts at: 4194 4444 4513 4555 4630 4692 5169 5846 6994
Size: 250 69 42 75 62 477 677 1148
Cuts at: 6994 7239 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 743 721 677 477 444 254
250 245 228 111 75 69 66 62
42 21
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7201
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7753
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7753
NotI GC'GGCC_GC
Cuts at: 3012 5200 3012
Size: 2188 5565
NspI r_CATG'y
Cuts at: 1132 1229 3982 5111 6656 1132
Size: 97 2753 1129 1545 2229
Fragments arranged by size:
2753 2229 1545 1129 97
PasI CC'CwG_GG
Cuts at: 1348 2392 3187 3402 3553 4650 4991 1348
Size: 1044 795 215 151 1097 341 4110
Fragments arranged by size:
4110 1097 1044 795 341 215 151
PciI A'CATG_T
Cuts at: 6652 6652
Size: 7753
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3865 1108
Size: 2757 4996
PfoI T'CCnGG_A
Cuts at: 4275 4275
Size: 7753
PmeI GTTT'AAAC
Cuts at: 5057 5057
Size: 7753
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5822 5854 7335 7367 5822
Size: 32 1481 32 6208
Fragments arranged by size:
6208 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2563 2759 3622 4654 4984 2563
Size: 196 863 1032 330 5332
Fragments arranged by size:
5332 1032 863 330 196
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7753
PsiI TTA'TAA
Cuts at: 5323 5323
Size: 7753
PspOMI G'GGCC_C
Cuts at: 3397 4418 3397
Size: 1021 6732
PspXI vC'TCGA_Gb
Cuts at: 2240 2888 4100 2240
Size: 648 1212 5893
Fragments arranged by size:
5893 1212 648
PstI C_TGCA'G
Cuts at: 830 2055 2106 2337 2841 3027 3063 3227 3453
Size: 1225 51 231 504 186 36 164 226
Cuts at: 3453 3762 3924 4518 4554 4572 4614 4635 5105
Size: 309 162 594 36 18 42 21 470
Cuts at: 5105 830
Size: 3478
Fragments arranged by size:
3478 1225 594 504 470 309 231 226
186 164 162 51 42 36 36 21
18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7753
PvuII CAG'CTG
Cuts at: 2050 2071 3763 3874 4194 4513 4555 4630 4692
Size: 21 1692 111 320 319 42 75 62
Cuts at: 4692 2050
Size: 5111
Fragments arranged by size:
5111 1692 320 319 111 75 62 42
21
RsrII CG'GwC_CG
Cuts at: 3214 3214
Size: 7753
SacI G_AGCT'C
Cuts at: 2241 2889 3039 4359 4895 2241
Size: 648 150 1320 536 5099
Fragments arranged by size:
5099 1320 648 536 150
SalI G'TCGA_C
Cuts at: 1936 5095 1936
Size: 3159 4594
SapI GCTCTTCn'nnn_
Cuts at: 2280 4031 2280
Size: 1751 6002
SbfI CC_TGCA'GG
Cuts at: 5105 5105
Size: 7753
ScaI AGT'ACT
Cuts at: 6001 7713 6001
Size: 1712 6041
SfcI C'TryA_G
Cuts at: 826 1045 2051 2102 2333 2837 3023 3059 3223
Size: 219 1006 51 231 504 186 36 164
Cuts at: 3223 3449 3758 3920 4439 4483 4514 4550 4568
Size: 226 309 162 519 44 31 36 18
Cuts at: 4568 4610 4631 5101 6236 6917 7108 826
Size: 42 21 470 1135 681 191 1471
Fragments arranged by size:
1471 1135 1006 681 519 504 470 309
231 226 219 191 186 164 162 51
44 42 36 36 31 21 18
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7753
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7753
SmaI CCC'GGG
Cuts at: 1872 4850 5080 1872
Size: 2978 230 4545
Fragments arranged by size:
4545 2978 230
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2240 2888 4049 4100 4358 5857
Size: 197 926 297 648 1161 51 258 1499
Cuts at: 5857 6758 7020 820
Size: 901 262 1553
Fragments arranged by size:
1553 1499 1161 926 901 648 297 262
258 197 51
SnaBI TAC'GTA
Cuts at: 493 5544 493
Size: 5051 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7753
SphI G_CATG'C
Cuts at: 1132 5111 1132
Size: 3979 3774
SspI AAT'ATT
Cuts at: 5 52 5677 5
Size: 47 5625 2081
Fragments arranged by size:
5625 2081 47
StuI AGG'CCT
Cuts at: 1517 2709 3709 4178 1517
Size: 1192 1000 469 5092
Fragments arranged by size:
5092 1192 1000 469
StyI C'CwwG_G
Cuts at: 513 1065 2508 3543 3589 4320 4326 4923 5192
Size: 552 1443 1035 46 731 6 597 269
Cuts at: 5192 513
Size: 3074
Fragments arranged by size:
3074 1443 1035 731 597 552 269 46
6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2368 2636 2780 4955 5780 5797 5950
Size: 1255 788 268 144 2175 825 17 153
Cuts at: 5950 6135 6580 325
Size: 185 445 1498
Fragments arranged by size:
2175 1498 1255 825 788 445 268 185
153 144 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4125 4218 5999
Size: 274 80 33 51 1443 2148 93 1781
Cuts at: 5999 7711 96
Size: 1712 138
Fragments arranged by size:
2148 1781 1712 1443 274 138 93 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3338 3370 7713 7745 3338
Size: 32 4343 32 3346
Fragments arranged by size:
4343 3346 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7753
XbaI T'CTAG_A
Cuts at: 5089 5089
Size: 7753
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3295 3295
Size: 7753
XhoI C'TCGA_G
Cuts at: 1943 2240 2888 4100 1943
Size: 297 648 1212 5596
Fragments arranged by size:
5596 1212 648 297
XmaI C'CCGG_G
Cuts at: 1870 4848 5078 1870
Size: 2978 230 4545
Fragments arranged by size:
4545 2978 230
XmnI GAAnn'nnTTC
Cuts at: 2289 3467 5882 2289
Size: 1178 2415 4160
Fragments arranged by size:
4160 2415 1178
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2023 5561 276
Size: 53 83 186 936 489 3538 2468
Fragments arranged by size:
3538 2468 936 489 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI
BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PsrI SacII SanDI SexAI
SfiI SfoI SrfI SwaI