Restriction map for
pFN21AB7348
(Circular) (Six-base) MAPSORT of: pFN21AB7348.seq Check: 854 from: 1 to: 8062
pFN21AB7348 8062 bp
With 182 enzymes: SgfI *
May 6, 2010 17:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4930 4930
Size: 8062
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5872 278
Size: 53 83 186 936 4336 2468
Fragments arranged by size:
4336 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2686 3108 3268 4622 5405 1937
Size: 749 422 160 1354 783 4594
Fragments arranged by size:
4594 1354 783 749 422 160
Acc65I G'GTAC_C
Cuts at: 5383 5383
Size: 8062
AclI AA'CG_TT
Cuts at: 1563 6189 6562 1563
Size: 4626 373 3063
Fragments arranged by size:
4626 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3045 4681 4714 5177 6125 7509 7777 1632
Size: 1413 1636 33 463 948 1384 268 1917
Fragments arranged by size:
1917 1636 1413 1384 948 463 268 33
AflII C'TTAA_G
Cuts at: 820 1017 2111 3830 820
Size: 197 1094 1719 5052
Fragments arranged by size:
5052 1719 1094 197
AflIII A'CryG_T
Cuts at: 1203 6961 7938 1203
Size: 5758 977 1327
Fragments arranged by size:
5758 1327 977
AgeI A'CCGG_T
Cuts at: 6893 6893
Size: 8062
AhdI GACnn_n'nnGTC
Cuts at: 6791 6791
Size: 8062
AleI CACnn'nnGTG
Cuts at: 1166 2507 5015 1166
Size: 1341 2508 4213
Fragments arranged by size:
4213 2508 1341
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2229 1184
Size: 1045 7017
AlwNI CAG_nnn'CTG
Cuts at: 5805 7377 5805
Size: 1572 6490
ApaI G_GGCC'C
Cuts at: 3281 3281
Size: 8062
ApaLI G'TGCA_C
Cuts at: 2521 2790 2821 3622 6119 7275 2521
Size: 269 31 801 2497 1156 3308
Fragments arranged by size:
3308 2497 1156 801 269 31
ApoI r'AATT_y
Cuts at: 1493 3079 3188 4007 4168 4300 4377 4537 4575
Size: 1586 109 819 161 132 77 160 38
Cuts at: 4575 4681 4864 5371 5597 5749 7858 1493
Size: 106 183 507 226 152 2109 1697
Fragments arranged by size:
2109 1697 1586 819 507 226 183 161
160 152 132 109 106 77 38
AseI AT'TA_AT
Cuts at: 160 1032 3009 6616 160
Size: 872 1977 3607 1606
Fragments arranged by size:
3607 1977 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8062
AvaI C'yCGr_G
Cuts at: 1870 1943 5387 1870
Size: 73 3444 4545
Fragments arranged by size:
4545 3444 73
BamHI G'GATC_C
Cuts at: 4690 5392 5759 4690
Size: 702 367 6993
Fragments arranged by size:
6993 702 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2031 3098 5383 6838 618
Size: 325 217 627 244 1067 2285 1455 1842
Fragments arranged by size:
2285 1842 1455 1067 627 325 244 217
BanII G_rGCy'C
Cuts at: 1391 2055 3281 4159 5196 5381 1391
Size: 664 1226 878 1037 185 4072
Fragments arranged by size:
4072 1226 1037 878 664 185
BbeI G_GCGC'C
Cuts at: 2035 2035
Size: 8062
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3237 3648 3951 928
Size: 971 1338 411 303 5039
Fragments arranged by size:
5039 1338 971 411 303
BbvCI CC'TCA_GC
Cuts at: 4899 4899
Size: 8062
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6253 6287 1903
Size: 34 4316 34 3678
Fragments arranged by size:
4316 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5031 5954 7164 5031
Size: 923 1210 5929
Fragments arranged by size:
5929 1210 923
BclI T'GATC_A
Cuts at: 1548 2511 2766 2962 4273 5205 1548
Size: 963 255 196 1311 932 4405
Fragments arranged by size:
4405 1311 963 932 255 196
BfrBI ATG'CAT
Cuts at: 2643 3263 3903 4916 2643
Size: 620 640 1013 5789
Fragments arranged by size:
5789 1013 640 620
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6673 136
Size: 107 122 71 631 605 5001 1525
Fragments arranged by size:
5001 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5036 8057 5036
Size: 3021 5041
BlpI GC'TnA_GC
Cuts at: 2571 5479 2571
Size: 2908 5154
Bme1580I G_kGCm'C
Cuts at: 1790 2525 2794 2825 3103 3281 3626 6123 7279
Size: 735 269 31 278 178 345 2497 1156
Cuts at: 7279 1790
Size: 2573
Fragments arranged by size:
2573 2497 1156 735 345 278 269 178
31
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8062
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2798 5091 5793
Size: 416 53 83 399 24 1381 2293 702
Cuts at: 5793 6751 442
Size: 958 1753
Fragments arranged by size:
2293 1753 1381 958 702 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2978 1056
Size: 1922 6140
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5245 5245
Size: 8062
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2058 3837 6722 1239
Size: 819 1779 2885 2579
Fragments arranged by size:
2885 2579 1779 819
Bpu10I CC'TnA_GC
Cuts at: 4899 4899
Size: 8062
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2477 5375 6187 7052 7350 2477
Size: 2898 812 865 298 3189
Fragments arranged by size:
3189 2898 865 812 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2927 4196 6725 882
Size: 617 1428 1269 2529 2219
Fragments arranged by size:
2529 2219 1428 1269 617
BsaAI yAC'GTr
Cuts at: 493 1204 5853 493
Size: 711 4649 2702
Fragments arranged by size:
4649 2702 711
BsaBI GATnn'nnATC
Cuts at: 5222 8056 5222
Size: 2834 5228
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2032 5869 6251 6886
Size: 53 83 186 936 499 3837 382 635
Cuts at: 6886 275
Size: 1451
Fragments arranged by size:
3837 1451 936 635 499 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6495 6893 7167 7314 7725 6495
Size: 398 274 147 411 6832
Fragments arranged by size:
6832 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7837
Fragments arranged by size:
7837 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2379 2394 3240 3447 1186
Size: 1193 15 846 207 5801
Fragments arranged by size:
5801 1193 846 207 15
BseYI C'CCAG_C
Cuts at: 7265 7761 7265
Size: 496 7566
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2441 3639 1163
Size: 573 705 1198 5586
Fragments arranged by size:
5586 1198 705 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5512 6273 7301 1540
Size: 275 186 3511 761 1028 2301
Fragments arranged by size:
3511 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2525 2794 2825 3626 4159 5196 6123 6208 7279
Size: 269 31 801 533 1037 927 85 1071
Cuts at: 7279 2525
Size: 3308
Fragments arranged by size:
3308 1071 1037 927 801 533 269 85
31
BsmI GAATG_Cn'
Cuts at: 2645 3905 3934 5573 5666 2645
Size: 1260 29 1639 93 5041
Fragments arranged by size:
5041 1639 1260 93 29
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 2525 2794 2825 3103 3281 3626
Size: 399 265 470 269 31 278 178 345
Cuts at: 3626 4159 5196 5381 6123 6208 7279 1391
Size: 533 1037 185 742 85 1071 2174
Fragments arranged by size:
2174 1071 1037 742 533 470 399 345
278 269 265 185 178 85 31
BspEI T'CCGG_A
Cuts at: 7725 7725
Size: 8062
BspHI T'CATG_A
Cuts at: 2929 4128 4860 5951 7681 2929
Size: 1199 732 1091 1730 3310
Fragments arranged by size:
3310 1730 1199 1091 732
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4930 5417 844
Size: 4086 487 3489
Fragments arranged by size:
4086 3489 487
BsrBI CCG'CTC
Cuts at: 1394 1823 5949 1394
Size: 429 4126 3507
Fragments arranged by size:
4126 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2514 2543 4074 4423 4508 4706 4893
Size: 1183 1266 29 1531 349 85 198 187
Cuts at: 4893 6557 6731 65
Size: 1664 174 1396
Fragments arranged by size:
1664 1531 1396 1266 1183 349 198 187
174 85 29
BsrFI r'CCGG_y
Cuts at: 1700 1810 5151 6706 6893 1700
Size: 110 3341 1555 187 2869
Fragments arranged by size:
3341 2869 1555 187 110
BsrGI T'GTAC_A
Cuts at: 96 3151 96
Size: 3055 5007
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8062
BssSI C'ACGA_G
Cuts at: 6122 7134 6122
Size: 1012 7050
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2554 794
Size: 1760 6302
BstBI TT'CG_AA
Cuts at: 6866 6866
Size: 8062
BstEII G'GTnAC_C
Cuts at: 1184 3437 1184
Size: 2253 5809
BstYI r'GATC_y
Cuts at: 1972 4175 4690 5036 5161 5392 5759 6144 6161
Size: 2203 515 346 125 231 367 385 17
Cuts at: 6161 7613 7699 7711 8057 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2203 1977 1452 515 385 367 346 346
231 125 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2018 2018
Size: 8062
BtgI C'CryG_G
Cuts at: 513 1065 3570 513
Size: 552 2505 5005
Fragments arranged by size:
5005 2505 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7323
Fragments arranged by size:
7323 683 56
BtsI GCAGTG_nn'
Cuts at: 3651 4502 5578 6372 6392 3651
Size: 851 1076 794 20 5321
Fragments arranged by size:
5321 1076 851 794 20
ClaI AT'CG_AT
Cuts at: 1554 3018 1554
Size: 1464 6598
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2845 2880 529
Size: 35 2281 35 5711
Fragments arranged by size:
5711 2281 35 35
DraI TTT'AAA
Cuts at: 5366 5366
Size: 8062
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7069 809
Size: 507 5753 1802
Fragments arranged by size:
5753 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5509 6398 8027
Size: 54 1425 50 275 18 3679 889 1629
Cuts at: 8027 8
Size: 43
Fragments arranged by size:
3679 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5509 1537
Size: 275 3697 4090
Fragments arranged by size:
4090 3697 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2664 4919 5992 1353
Size: 1311 2255 1073 3423
Fragments arranged by size:
3423 2255 1311 1073
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6649 7023 7169 387
Size: 1064 138 5060 374 146 1280
Fragments arranged by size:
5060 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4157 5194 4157
Size: 1037 7025
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2058 3045 3837 4681 4714 5177 6125
Size: 393 426 987 792 844 33 463 948
Cuts at: 6125 6722 7509 7777 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 987 948 844 792 787 597 463
426 393 268 33
EcoNI CCTnn'n_nnAGG
Cuts at: 3391 3391
Size: 8062
EcoO109I rG'GnC_Cy
Cuts at: 3277 3278 3277
Size: 1 8061
EcoRI G'AATT_C
Cuts at: 4007 4300 5371 5749 4007
Size: 293 1071 378 6320
Fragments arranged by size:
6320 1071 378 293
EcoRV GAT'ATC
Cuts at: 2880 4750 2880
Size: 1870 6192
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3458 3458
Size: 8062
FspI TGC'GCA
Cuts at: 6568 6568
Size: 8062
HaeII r_GCGC'y
Cuts at: 1678 2035 7209 1678
Size: 357 5174 2531
Fragments arranged by size:
5174 2531 357
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2389 2421 3329 3361 3826
Size: 32 135 32 817 32 908 32 465
Cuts at: 3826 3858 4991 5023 6703 6735 6777 6809 1373
Size: 32 1133 32 1680 32 42 32 2626
Fragments arranged by size:
2626 1680 1133 908 817 465 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2911 5406 5652 669
Size: 988 281 973 2495 246 3079
Fragments arranged by size:
3079 2495 988 973 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8062
HpaI GTT'AAC
Cuts at: 2911 5652 2911
Size: 2741 5321
KasI G'GCGC_C
Cuts at: 2031 2031
Size: 8062
KpnI G_GTAC'C
Cuts at: 5387 5387
Size: 8062
MfeI C'AATT_G
Cuts at: 4512 4851 5661 4512
Size: 339 810 6913
Fragments arranged by size:
6913 810 339
MluI A'CGCG_T
Cuts at: 7938 7938
Size: 8062
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4408 4572 7176 7360 1305
Size: 3103 164 2604 184 2007
Fragments arranged by size:
3103 2604 2007 184 164
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6240
Fragments arranged by size:
6240 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2417 2507 4199 4457 5015 6020 6379
Size: 648 1251 90 1692 258 558 1005 359
Cuts at: 6379 6538 518
Size: 159 2042
Fragments arranged by size:
2042 1692 1251 1005 648 558 359 258
159 90
MspA1I CmG'CkG
Cuts at: 1606 2507 5478 6155 7303 7548 1606
Size: 901 2971 677 1148 245 2120
Fragments arranged by size:
2971 2120 1148 901 677 245
NarI GG'CG_CC
Cuts at: 2032 2032
Size: 8062
NcoI C'CATG_G
Cuts at: 513 1065 3570 513
Size: 552 2505 5005
Fragments arranged by size:
5005 2505 552
NdeI CA'TA_TG
Cuts at: 387 2137 4161 387
Size: 1750 2024 4288
Fragments arranged by size:
4288 2024 1750
NheI G'CTAG_C
Cuts at: 1052 2974 1052
Size: 1922 6140
NotI GC'GGCC_GC
Cuts at: 5509 5509
Size: 8062
NsiI A_TGCA'T
Cuts at: 2645 3265 3905 4918 2645
Size: 620 640 1013 5789
Fragments arranged by size:
5789 1013 640 620
NspI r_CATG'y
Cuts at: 1132 1229 3267 3931 4916 5420 6965 1132
Size: 97 2038 664 985 504 1545 2229
Fragments arranged by size:
2229 2038 1545 985 664 504 97
PasI CC'CwG_GG
Cuts at: 1348 2056 2848 1348
Size: 708 792 6562
Fragments arranged by size:
6562 792 708
PciI A'CATG_T
Cuts at: 6961 6961
Size: 8062
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8062
PfoI T'CCnGG_A
Cuts at: 5175 5175
Size: 8062
PmeI GTTT'AAAC
Cuts at: 5366 5366
Size: 8062
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6131 6163 7644 7676 6131
Size: 32 1481 32 6517
Fragments arranged by size:
6517 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8062
PsiI TTA'TAA
Cuts at: 5632 5632
Size: 8062
PspOMI G'GGCC_C
Cuts at: 3277 3277
Size: 8062
PstI C_TGCA'G
Cuts at: 830 2218 4497 5414 830
Size: 1388 2279 917 3478
Fragments arranged by size:
3478 2279 1388 917
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8062
PvuII CAG'CTG
Cuts at: 2507 2507
Size: 8062
SacI G_AGCT'C
Cuts at: 4159 5196 4159
Size: 1037 7025
SalI G'TCGA_C
Cuts at: 1936 5404 1936
Size: 3468 4594
SapI GCTCTTCn'nnn_
Cuts at: 2664 2664
Size: 8062
SbfI CC_TGCA'GG
Cuts at: 5414 5414
Size: 8062
ScaI AGT'ACT
Cuts at: 6310 8022 6310
Size: 1712 6350
SfcI C'TryA_G
Cuts at: 826 1045 2214 2319 4493 4623 5410 6545 7226
Size: 219 1169 105 2174 130 787 1135 681
Cuts at: 7226 7417 826
Size: 191 1471
Fragments arranged by size:
2174 1471 1169 1135 787 681 219 191
130 105
SfoI GGC'GCC
Cuts at: 2033 2033
Size: 8062
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8062
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8062
SmaI CCC'GGG
Cuts at: 1872 5389 1872
Size: 3517 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2111 2456 3830 5354 6166 7067
Size: 197 926 168 345 1374 1524 812 901
Cuts at: 7067 7329 820
Size: 262 1553
Fragments arranged by size:
1553 1524 1374 926 901 812 345 262
197 168
SnaBI TAC'GTA
Cuts at: 493 5853 493
Size: 5360 2702
SpeI A'CTAG_T
Cuts at: 152 4268 152
Size: 4116 3946
SphI G_CATG'C
Cuts at: 1132 5420 1132
Size: 4288 3774
SspI AAT'ATT
Cuts at: 5 52 3073 4121 4842 5136 5986 5
Size: 47 3021 1048 721 294 850 2081
Fragments arranged by size:
3021 2081 1048 850 721 294 47
StuI AGG'CCT
Cuts at: 1517 3746 1517
Size: 2229 5833
StyI C'CwwG_G
Cuts at: 513 1065 3570 5501 513
Size: 552 2505 1931 3074
Fragments arranged by size:
3074 2505 1931 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2861 3231 6089 6106 6259 6444 6889
Size: 1255 1281 370 2858 17 153 185 445
Cuts at: 6889 325
Size: 1498
Fragments arranged by size:
2858 1498 1281 1255 445 370 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3151 3245 6308
Size: 274 80 33 51 1443 1174 94 3063
Cuts at: 6308 8020 96
Size: 1712 138
Fragments arranged by size:
3063 1712 1443 1174 274 138 94 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8022 8054 8022
Size: 32 8030
Tth111I GACn'n_nGTC
Cuts at: 1316 3725 1316
Size: 2409 5653
XbaI T'CTAG_A
Cuts at: 5398 5398
Size: 8062
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2641 2641
Size: 8062
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8062
XmaI C'CCGG_G
Cuts at: 1870 5387 1870
Size: 3517 4545
XmnI GAAnn'nnTTC
Cuts at: 6191 6191
Size: 8062
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5870 276
Size: 53 83 186 936 4336 2468
Fragments arranged by size:
4336 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BsiWI BsmBI BstXI
BstZ17I DraIII FseI FspAI NaeI NgoMIV NruI PacI
PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SrfI SwaI