Restriction map for
pFN21AB7345
(Circular) (Six-base) MAPSORT of: pFN21AB7345.seq Check: 8759 from: 1 to: 11374
pFN21AB7345 11374 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6454 6454
Size: 11374
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 9184 278
Size: 53 83 186 936 7648 2468
Fragments arranged by size:
7648 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3276 8717 1937
Size: 1339 5441 4594
Fragments arranged by size:
5441 4594 1339
Acc65I G'GTAC_C
Cuts at: 6368 8695 6368
Size: 2327 9047
AclI AA'CG_TT
Cuts at: 1563 4492 9501 9874 1563
Size: 2929 5009 373 3063
Fragments arranged by size:
5009 3063 2929 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2182 2742 2807 3156 3240 3291 3396 3546
Size: 550 560 65 349 84 51 105 150
Cuts at: 3546 3845 4062 4370 5463 6219 6717 7542 8025
Size: 299 217 308 1093 756 498 825 483
Cuts at: 8025 9437 10821 11089 1632
Size: 1412 1384 268 1917
Fragments arranged by size:
1917 1412 1384 1093 825 756 560 550
498 483 349 308 299 268 217 150
105 84 65 51
AfeI AGC'GCT
Cuts at: 4859 4859
Size: 11374
AflII C'TTAA_G
Cuts at: 820 1017 4861 7361 8267 820
Size: 197 3844 2500 906 3927
Fragments arranged by size:
3927 3844 2500 906 197
AflIII A'CryG_T
Cuts at: 1203 6916 8671 10273 11250 1203
Size: 5713 1755 1602 977 1327
Fragments arranged by size:
5713 1755 1602 1327 977
AgeI A'CCGG_T
Cuts at: 10205 10205
Size: 11374
AhdI GACnn_n'nnGTC
Cuts at: 10103 10103
Size: 11374
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 11374
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3566 8079 1184
Size: 2382 4513 4479
Fragments arranged by size:
4513 4479 2382
AlwNI CAG_nnn'CTG
Cuts at: 3203 3890 4148 5636 6278 6531 9117 10689 3203
Size: 687 258 1488 642 253 2586 1572 3888
Fragments arranged by size:
3888 2586 1572 1488 687 642 258 253
ApaLI G'TGCA_C
Cuts at: 9431 10587 9431
Size: 1156 10218
ApoI r'AATT_y
Cuts at: 1493 2393 2414 3320 3625 3680 4033 4701 7987
Size: 900 21 906 305 55 353 668 3286
Cuts at: 7987 8034 8683 8909 9061 11170 1493
Size: 47 649 226 152 2109 1697
Fragments arranged by size:
3286 2109 1697 906 900 668 649 353
305 226 152 55 47 21
AseI AT'TA_AT
Cuts at: 160 1032 2235 3120 8202 9928 160
Size: 872 1203 885 5082 1726 1606
Fragments arranged by size:
5082 1726 1606 1203 885 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11374
AvaI C'yCGr_G
Cuts at: 1870 1943 6303 7261 8699 1870
Size: 73 4360 958 1438 4545
Fragments arranged by size:
4545 4360 1438 958 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5310 5343 7116 7149 5310
Size: 33 1773 33 9535
Fragments arranged by size:
9535 1773 33 33
BamHI G'GATC_C
Cuts at: 5446 5903 6757 8704 9071 5446
Size: 457 854 1947 367 7749
Fragments arranged by size:
7749 1947 854 457 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2657 5431 6176 6215
Size: 325 217 627 221 649 2774 745 39
Cuts at: 6215 6368 7058 7265 8695 10150 618
Size: 153 690 207 1430 1455 1842
Fragments arranged by size:
2774 1842 1455 1430 745 690 649 627
325 221 217 207 153 39
BanII G_rGCy'C
Cuts at: 1391 5703 7283 8028 8693 1391
Size: 4312 1580 745 665 4072
Fragments arranged by size:
4312 4072 1580 745 665
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2466 2619 6315 7919 8638 928
Size: 971 567 153 3696 1604 719 3664
Fragments arranged by size:
3696 3664 1604 971 719 567 153
BbvCI CC'TCA_GC
Cuts at: 2184 2697 2184
Size: 513 10861
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4363 4397 9565 9599 1903
Size: 34 2426 34 5168 34 3678
Fragments arranged by size:
5168 3678 2426 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5060 5130 9266 10476 5060
Size: 70 4136 1210 5958
Fragments arranged by size:
5958 4136 1210 70
BclI T'GATC_A
Cuts at: 1548 6424 1548
Size: 4876 6498
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 9985 136
Size: 107 122 71 631 605 8313 1525
Fragments arranged by size:
8313 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2833 3382 11369 2833
Size: 549 7987 2838
Fragments arranged by size:
7987 2838 549
BlpI GC'TnA_GC
Cuts at: 2718 4191 4650 8791 2718
Size: 1473 459 4141 5301
Fragments arranged by size:
5301 4141 1473 459
Bme1580I G_kGCm'C
Cuts at: 1790 2662 3029 3336 3555 7261 9435 10591 1790
Size: 872 367 307 219 3706 2174 1156 2573
Fragments arranged by size:
3706 2573 2174 1156 872 367 307 219
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 11374
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2587 3390 5688
Size: 416 53 83 399 24 1170 803 2298
Cuts at: 5688 7278 8038 9105 10063 442
Size: 1590 760 1067 958 1753
Fragments arranged by size:
2298 1753 1590 1170 1067 958 803 760
416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 4698 1056
Size: 3642 7732
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2210 2390 4232 4369 6117 7176 10034 1239
Size: 971 180 1842 137 1748 1059 2858 2579
Fragments arranged by size:
2858 2579 1842 1748 1059 971 180 137
Bpu10I CC'TnA_GC
Cuts at: 2184 2697 2184
Size: 513 10861
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2978 3793 3957 4799 7259 7451 7897 8288 9499
Size: 815 164 842 2460 192 446 391 1211
Cuts at: 9499 10364 10662 2978
Size: 865 298 3690
Fragments arranged by size:
3690 2460 1211 865 842 815 446 391
298 192 164
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 10037 882
Size: 617 8538 2219
Fragments arranged by size:
8538 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 9165 493
Size: 711 7961 2702
Fragments arranged by size:
7961 2702 711
BsaBI GATnn'nnATC
Cuts at: 5445 7869 11368 5445
Size: 2424 3499 5451
Fragments arranged by size:
5451 3499 2424
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 9181 9563 10198 275
Size: 53 83 186 936 7648 382 635 1451
Fragments arranged by size:
7648 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 7146 9807 10205 10479 10626 11037 7146
Size: 2661 398 274 147 411 7483
Fragments arranged by size:
7483 2661 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2019 2049 3644 3674 6139
Size: 30 165 30 616 30 1595 30 2465
Cuts at: 6139 6169 7984 8014 8154 8184 1178
Size: 30 1815 30 140 30 4368
Fragments arranged by size:
4368 2465 1815 1595 616 165 140 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2622 4868 6120 7451 7454 1186
Size: 1436 2246 1252 1331 3 5106
Fragments arranged by size:
5106 2246 1436 1331 1252 3
BseYI C'CCAG_C
Cuts at: 8354 10577 11073 8354
Size: 2223 496 8655
Fragments arranged by size:
8655 2223 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2061 7064 1163
Size: 573 325 5003 5473
Fragments arranged by size:
5473 5003 573 325
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8824 9585 10613 1540
Size: 275 186 6823 761 1028 2301
Fragments arranged by size:
6823 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 9435 9520 10591 9435
Size: 85 1071 10218
Fragments arranged by size:
10218 1071 85
BsmI GAATG_Cn'
Cuts at: 2727 4114 8885 8978 2727
Size: 1387 4771 93 5123
Fragments arranged by size:
5123 4771 1387 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2662 3029 3336 3555 5703 7261 7283
Size: 399 872 367 307 219 2148 1558 22
Cuts at: 7283 8028 8693 9435 9520 10591 1391
Size: 745 665 742 85 1071 2174
Fragments arranged by size:
2174 2148 1558 1071 872 745 742 665
399 367 307 219 85 22
BspEI T'CCGG_A
Cuts at: 11037 11037
Size: 11374
BspHI T'CATG_A
Cuts at: 3508 3580 3739 3957 4182 4267 6720 9263 10993
Size: 72 159 218 225 85 2453 2543 1730
Cuts at: 10993 3508
Size: 3889
Fragments arranged by size:
3889 2543 2453 1730 225 218 159 85
72
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3196 6454 6702 7342 8319 8729 844
Size: 2352 3258 248 640 977 410 3489
Fragments arranged by size:
3489 3258 2352 977 640 410 248
BsrBI CCG'CTC
Cuts at: 1394 1823 9261 1394
Size: 429 7438 3507
Fragments arranged by size:
7438 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2109 4515 7456 9869 10043 65
Size: 1183 861 2406 2941 2413 174 1396
Fragments arranged by size:
2941 2413 2406 1396 1183 861 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 10018 10205 1700
Size: 110 8208 187 2869
Fragments arranged by size:
8208 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 11374
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11374
BssSI C'ACGA_G
Cuts at: 9434 10446 9434
Size: 1012 10362
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3352 3655 5930 8195 794
Size: 2558 303 2275 2265 3973
Fragments arranged by size:
3973 2558 2275 2265 303
BstBI TT'CG_AA
Cuts at: 10178 10178
Size: 11374
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 11374
BstYI r'GATC_y
Cuts at: 1972 2258 2833 3382 4081 5001 5363 5446 5903
Size: 286 575 549 699 920 362 83 457
Cuts at: 5903 6274 6757 8704 9071 9456 9473 10925 11011
Size: 371 483 1947 367 385 17 1452 86
Cuts at: 11011 11023 11369 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1947 1452 920 699 575 549 483
457 385 371 367 362 346 286 86
83 17 12
Bsu36I CC'TnA_GG
Cuts at: 2562 5238 6762 2562
Size: 2676 1524 7174
Fragments arranged by size:
7174 2676 1524
BtgI C'CryG_G
Cuts at: 513 1065 2004 2166 3707 3908 7103 513
Size: 552 939 162 1541 201 3195 4784
Fragments arranged by size:
4784 3195 1541 939 552 201 162
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 10635
Fragments arranged by size:
10635 683 56
BtsI GCAGTG_nn'
Cuts at: 3555 7965 8890 9684 9704 3555
Size: 4410 925 794 20 5225
Fragments arranged by size:
5225 4410 925 794 20
ClaI AT'CG_AT
Cuts at: 1554 4840 1554
Size: 3286 8088
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 8425 8460 529
Size: 35 7861 35 3443
Fragments arranged by size:
7861 3443 35 35
DraI TTT'AAA
Cuts at: 4369 5229 6840 7741 8335 8678 4369
Size: 860 1611 901 594 343 7065
Fragments arranged by size:
7065 1611 901 860 594 343
DraIII CAC_nnn'GTG
Cuts at: 3457 3457
Size: 11374
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5774 6317 10381 809
Size: 507 4458 543 4064 1802
Fragments arranged by size:
4458 4064 1802 543 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 8821 9710 11339
Size: 54 1425 50 275 18 6991 889 1629
Cuts at: 11339 8
Size: 43
Fragments arranged by size:
6991 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 8821 1537
Size: 275 7009 4090
Fragments arranged by size:
7009 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2201 4824 4845 5013 5253 5277 5598 9304
Size: 848 2623 21 168 240 24 321 3706
Cuts at: 9304 1353
Size: 3423
Fragments arranged by size:
3706 3423 2623 848 321 240 168 24
21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 9961 10335 10481 387
Size: 1064 138 8372 374 146 1280
Fragments arranged by size:
8372 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2182 2210 2390 2742 2807 3156 3240
Size: 393 550 28 180 352 65 349 84
Cuts at: 3240 3291 3396 3546 3845 4062 4232 4369 4370
Size: 51 105 150 299 217 170 137 1
Cuts at: 4370 5463 6117 6219 6717 7176 7542 8025 9437
Size: 1093 654 102 498 459 366 483 1412
Cuts at: 9437 10034 10821 11089 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1412 1093 787 654 597 550 498
483 459 393 366 352 349 299 268
217 180 170 150 137 105 102 84
65 51 28 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2538 2538
Size: 11374
EcoO109I rG'GnC_Cy
Cuts at: 2179 3560 4390 6443 7196 2179
Size: 1381 830 2053 753 6357
Fragments arranged by size:
6357 2053 1381 830 753
EcoRI G'AATT_C
Cuts at: 3320 8683 9061 3320
Size: 5363 378 5633
Fragments arranged by size:
5633 5363 378
EcoRV GAT'ATC
Cuts at: 4755 4755
Size: 11374
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2162 2837 4400 5591 2162
Size: 675 1563 1191 7945
Fragments arranged by size:
7945 1563 1191 675
FspI TGC'GCA
Cuts at: 9880 9880
Size: 11374
HaeII r_GCGC'y
Cuts at: 1678 4861 10521 1678
Size: 3183 5660 2531
Fragments arranged by size:
5660 3183 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3644 3676 4744 4776 4931
Size: 32 135 32 2072 32 1068 32 155
Cuts at: 4931 4963 5506 5538 5929 5961 6340 6372 6854
Size: 32 543 32 391 32 379 32 482
Cuts at: 6854 6886 10015 10047 10089 10121 1373
Size: 32 3129 32 42 32 2626
Fragments arranged by size:
3129 2626 2072 1068 543 482 391 379
155 135 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2325 2800 5709 8718 8964 669
Size: 988 281 387 475 2909 3009 246 3079
Fragments arranged by size:
3079 3009 2909 988 475 387 281 246
HindIII A'AGCT_T
Cuts at: 748 5346 748
Size: 4598 6776
HpaI GTT'AAC
Cuts at: 2325 2800 8964 2325
Size: 475 6164 4735
Fragments arranged by size:
6164 4735 475
KpnI G_GTAC'C
Cuts at: 6372 8699 6372
Size: 2327 9047
MfeI C'AATT_G
Cuts at: 7128 8973 7128
Size: 1845 9529
MluI A'CGCG_T
Cuts at: 11250 11250
Size: 11374
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2236 4516 5516 5877 8063 8140 8293 8425
Size: 931 2280 1000 361 2186 77 153 132
Cuts at: 8425 10488 10672 1305
Size: 2063 184 2007
Fragments arranged by size:
2280 2186 2063 2007 1000 931 361 184
153 132 77
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 9552
Fragments arranged by size:
9552 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3329 3962 4530 5229 6111 6363 7102
Size: 648 2163 633 568 699 882 252 739
Cuts at: 7102 8283 9332 9691 9850 518
Size: 1181 1049 359 159 2042
Fragments arranged by size:
2163 2042 1181 1049 882 739 699 648
633 568 359 252 159
MspA1I CmG'CkG
Cuts at: 1606 2978 4162 6641 6700 7847 8399 8441 8790
Size: 1372 1184 2479 59 1147 552 42 349
Cuts at: 8790 9467 10615 10860 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2479 2120 1372 1184 1148 1147 677 552
349 245 59 42
NcoI C'CATG_G
Cuts at: 513 1065 2004 3707 7103 513
Size: 552 939 1703 3396 4784
Fragments arranged by size:
4784 3396 1703 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 11374
NheI G'CTAG_C
Cuts at: 1052 4694 1052
Size: 3642 7732
NotI GC'GGCC_GC
Cuts at: 8821 8821
Size: 11374
NspI r_CATG'y
Cuts at: 1132 1229 3112 6920 8675 8732 10277 1132
Size: 97 1883 3808 1755 57 1545 2229
Fragments arranged by size:
3808 2229 1883 1755 1545 97 57
PasI CC'CwG_GG
Cuts at: 1348 8584 1348
Size: 7236 4138
PciI A'CATG_T
Cuts at: 6916 8671 10273 6916
Size: 1755 1602 8017
Fragments arranged by size:
8017 1755 1602
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2654 3203 1108
Size: 1546 549 9279
Fragments arranged by size:
9279 1546 549
PfoI T'CCnGG_A
Cuts at: 3069 3202 7192 3069
Size: 133 3990 7251
Fragments arranged by size:
7251 3990 133
PmeI GTTT'AAAC
Cuts at: 8678 8678
Size: 11374
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2052 2084 8165 8197 8501 8533 9443 9475 10956
Size: 32 6081 32 304 32 910 32 1481
Cuts at: 10956 10988 2052
Size: 32 2438
Fragments arranged by size:
6081 2438 1481 910 304 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2179 4390 6443 7196 2179
Size: 2211 2053 753 6357
Fragments arranged by size:
6357 2211 2053 753
PshAI GACnn'nnGTC
Cuts at: 1613 3257 1613
Size: 1644 9730
PsiI TTA'TAA
Cuts at: 8944 8944
Size: 11374
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5625 5657 5625
Size: 32 11342
PstI C_TGCA'G
Cuts at: 830 2781 7353 7407 7951 8058 8330 8726 830
Size: 1951 4572 54 544 107 272 396 3478
Fragments arranged by size:
4572 3478 1951 544 396 272 107 54
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11374
PvuII CAG'CTG
Cuts at: 2978 4162 6641 6700 7847 8399 8441 2978
Size: 1184 2479 59 1147 552 42 5911
Fragments arranged by size:
5911 2479 1184 1147 552 59 42
SalI G'TCGA_C
Cuts at: 1936 8716 1936
Size: 6780 4594
SapI GCTCTTCn'nnn_
Cuts at: 4824 4824
Size: 11374
SbfI CC_TGCA'GG
Cuts at: 8726 8726
Size: 11374
ScaI AGT'ACT
Cuts at: 2897 4380 9622 11334 2897
Size: 1483 5242 1712 2937
Fragments arranged by size:
5242 2937 1712 1483
SexAI A'CCwGG_T
Cuts at: 6179 6588 6179
Size: 409 10965
SfcI C'TryA_G
Cuts at: 826 1045 2289 2777 3611 5730 7292 7349 7403
Size: 219 1244 488 834 2119 1562 57 54
Cuts at: 7403 7947 8054 8326 8722 9857 10538 10729 826
Size: 544 107 272 396 1135 681 191 1471
Fragments arranged by size:
2119 1562 1471 1244 1135 834 681 544
488 396 272 219 191 107 57 54
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11374
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11374
SmaI CCC'GGG
Cuts at: 1872 7263 8701 1872
Size: 5391 1438 4545
Fragments arranged by size:
5391 4545 1438
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2957 3772 3972 4814 4861 6303
Size: 197 926 1014 815 200 842 47 1442
Cuts at: 6303 7274 7361 7466 7912 8267 8303 9478 10379
Size: 971 87 105 446 355 36 1175 901
Cuts at: 10379 10641 820
Size: 262 1553
Fragments arranged by size:
1553 1442 1175 1014 971 926 901 842
815 446 355 262 200 197 105 87
47 36
SnaBI TAC'GTA
Cuts at: 493 9165 493
Size: 8672 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11374
SphI G_CATG'C
Cuts at: 1132 3112 8732 1132
Size: 1980 5620 3774
Fragments arranged by size:
5620 3774 1980
SspI AAT'ATT
Cuts at: 5 52 3046 4508 5466 9298 5
Size: 47 2994 1462 958 3832 2081
Fragments arranged by size:
3832 2994 2081 1462 958 47
StuI AGG'CCT
Cuts at: 1517 2105 7227 1517
Size: 588 5122 5664
Fragments arranged by size:
5664 5122 588
StyI C'CwwG_G
Cuts at: 513 1065 2004 2505 3707 7103 8460 8813 513
Size: 552 939 501 1202 3396 1357 353 3074
Fragments arranged by size:
3396 3074 1357 1202 939 552 501 353
SwaI ATTT'AAAT
Cuts at: 5229 6840 5229
Size: 1611 9763
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2026 2606 3409 4457 5607 8368 9401
Size: 1255 446 580 803 1048 1150 2761 1033
Cuts at: 9401 9418 9571 9756 10201 325
Size: 17 153 185 445 1498
Fragments arranged by size:
2761 1498 1255 1150 1048 1033 803 580
446 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2895 2961 4378
Size: 274 80 33 51 1443 918 66 1417
Cuts at: 4378 6645 9620 11332 96
Size: 2267 2975 1712 138
Fragments arranged by size:
2975 2267 1712 1443 1417 918 274 138
80 66 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2019 2051 3443 3475 3862 3894 7619 7651 8154
Size: 32 1392 32 387 32 3725 32 503
Cuts at: 8154 8186 8355 8387 11334 11366 2019
Size: 32 169 32 2947 32 2027
Fragments arranged by size:
3725 2947 2027 1392 503 387 169 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 11374
XbaI T'CTAG_A
Cuts at: 2836 7873 8710 2836
Size: 5037 837 5500
Fragments arranged by size:
5500 5037 837
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2116 4288 4532 4651 2116
Size: 2172 244 119 8839
Fragments arranged by size:
8839 2172 244 119
XhoI C'TCGA_G
Cuts at: 1943 6303 1943
Size: 4360 7014
XmaI C'CCGG_G
Cuts at: 1870 7261 8699 1870
Size: 5391 1438 4545
Fragments arranged by size:
5391 4545 1438
XmnI GAAnn'nnTTC
Cuts at: 2817 3684 4788 6966 7167 7961 9503 2817
Size: 867 1104 2178 201 794 1542 4688
Fragments arranged by size:
4688 2178 1542 1104 867 794 201
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 9182 276
Size: 53 83 186 936 7648 2468
Fragments arranged by size:
7648 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI
PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I EcoICRI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
RsrII SacI SacII SanDI SfiI SfoI SrfI