Restriction map for
pFN21AB7341
(Circular) (Six-base) MAPSORT of: pFN21AB7341.seq Check: 8560 from: 1 to: 10237
pFN21AB7341 10237 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8047 278
Size: 53 83 186 936 6511 2468
Fragments arranged by size:
6511 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2892 5497 5544 6351 7272 7580 1937
Size: 955 2605 47 807 921 308 4594
Fragments arranged by size:
4594 2605 955 921 807 308 47
Acc65I G'GTAC_C
Cuts at: 7558 7558
Size: 10237
AclI AA'CG_TT
Cuts at: 1563 8364 8737 1563
Size: 6801 373 3063
Fragments arranged by size:
6801 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2784 3189 3437 3768 4062 4188 4298 4845
Size: 1152 405 248 331 294 126 110 547
Cuts at: 4845 5172 5309 5895 6441 8300 9684 9952 1632
Size: 327 137 586 546 1859 1384 268 1917
Fragments arranged by size:
1917 1859 1384 1152 586 547 546 405
331 327 294 268 248 137 126 110
AflII C'TTAA_G
Cuts at: 820 1017 4186 6398 820
Size: 197 3169 2212 4659
Fragments arranged by size:
4659 3169 2212 197
AflIII A'CryG_T
Cuts at: 1203 4618 6346 6969 9136 10113 1203
Size: 3415 1728 623 2167 977 1327
Fragments arranged by size:
3415 2167 1728 1327 977 623
AgeI A'CCGG_T
Cuts at: 9068 9068
Size: 10237
AhdI GACnn_n'nnGTC
Cuts at: 8966 8966
Size: 10237
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 10237
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 10237
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6395 6427 6395
Size: 32 10205
AlwNI CAG_nnn'CTG
Cuts at: 2276 2972 5287 6375 6605 7980 9552 2276
Size: 696 2315 1088 230 1375 1572 2961
Fragments arranged by size:
2961 2315 1572 1375 1088 696 230
ApaLI G'TGCA_C
Cuts at: 8294 9450 8294
Size: 1156 9081
ApoI r'AATT_y
Cuts at: 1493 2563 2683 2810 2940 3027 3349 3977 4688
Size: 1070 120 127 130 87 322 628 711
Cuts at: 4688 4748 4762 4817 4874 5017 5502 5797 6124
Size: 60 14 55 57 143 485 295 327
Cuts at: 6124 6318 6476 6497 6943 7198 7482 7546 7772
Size: 194 158 21 446 255 284 64 226
Cuts at: 7772 7924 10033 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1070 711 628 485 446 327
322 295 284 255 226 194 158 152
143 130 127 120 87 64 60 57
55 21 14
AseI AT'TA_AT
Cuts at: 160 1032 3935 8791 160
Size: 872 2903 4856 1606
Fragments arranged by size:
4856 2903 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10237
AvaI C'yCGr_G
Cuts at: 1870 1943 5323 7562 1870
Size: 73 3380 2239 4545
Fragments arranged by size:
4545 3380 2239 73
BamHI G'GATC_C
Cuts at: 7567 7934 7567
Size: 367 9870
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 7558 9013 618
Size: 325 217 627 5771 1455 1842
Fragments arranged by size:
5771 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3965 7556 1391
Size: 2574 3591 4072
Fragments arranged by size:
4072 3591 2574
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2153 2234 2439 3261 5075 5160 6429
Size: 971 254 81 205 822 1814 85 1269
Cuts at: 6429 928
Size: 4736
Fragments arranged by size:
4736 1814 1269 971 822 254 205 85
81
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8428 8462 1903
Size: 34 6491 34 3678
Fragments arranged by size:
6491 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3368 6203 8129 9339 3368
Size: 2835 1926 1210 4266
Fragments arranged by size:
4266 2835 1926 1210
BclI T'GATC_A
Cuts at: 1548 6763 1548
Size: 5215 5022
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2027 8848 136
Size: 107 122 71 631 605 355 6821 1525
Fragments arranged by size:
6821 1525 631 605 355 122 107 71
BglII A'GATC_T
Cuts at: 2647 3791 4028 4717 10232 2647
Size: 1144 237 689 5515 2652
Fragments arranged by size:
5515 2652 1144 689 237
BlpI GC'TnA_GC
Cuts at: 7654 7654
Size: 10237
Bme1580I G_kGCm'C
Cuts at: 1790 5443 8298 9454 1790
Size: 3653 2855 1156 2573
Fragments arranged by size:
3653 2855 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 10237
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2767 3477 7968
Size: 416 53 83 399 24 1350 710 4491
Cuts at: 7968 8926 442
Size: 958 1753
Fragments arranged by size:
4491 1753 1350 958 710 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10237
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7368 7368
Size: 10237
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4985 5084 7140 8897 1239
Size: 3746 99 2056 1757 2579
Fragments arranged by size:
3746 2579 2056 1757 99
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4868 6557 7106 8362 9227 9525 4868
Size: 1689 549 1256 865 298 5580
Fragments arranged by size:
5580 1689 1256 865 549 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6377 8900 882
Size: 617 4878 2523 2219
Fragments arranged by size:
4878 2523 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 8028 493
Size: 711 6824 2702
Fragments arranged by size:
6824 2702 711
BsaBI GATnn'nnATC
Cuts at: 6732 10231 6732
Size: 3499 6738
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 8044 8426 9061 275
Size: 53 83 186 936 6511 382 635 1451
Fragments arranged by size:
6511 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6821 8670 9068 9342 9489 9900 6821
Size: 1849 398 274 147 411 7158
Fragments arranged by size:
7158 1849 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 6216 6246 1178
Size: 30 165 30 4813 30 5169
Fragments arranged by size:
5169 4813 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4186 5933 1186
Size: 3000 1747 5490
Fragments arranged by size:
5490 3000 1747
BseYI C'CCAG_C
Cuts at: 2946 5484 5882 5948 9440 9936 2946
Size: 2538 398 66 3492 496 3247
Fragments arranged by size:
3492 3247 2538 496 398 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 6639 7451 1163
Size: 573 4903 812 3949
Fragments arranged by size:
4903 3949 812 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7687 8448 9476 1540
Size: 275 186 5686 761 1028 2301
Fragments arranged by size:
5686 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4926 5698 8298 8383 9454 4926
Size: 772 2600 85 1071 5709
Fragments arranged by size:
5709 2600 1071 772 85
BsmI GAATG_Cn'
Cuts at: 5479 5772 5965 7748 7841 5479
Size: 293 193 1783 93 7875
Fragments arranged by size:
7875 1783 293 193 93
BsmBI CGTCTCn'nnnn_
Cuts at: 6819 6819
Size: 10237
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3965 4926 5443 5698 7556 8298 8383
Size: 399 2175 961 517 255 1858 742 85
Cuts at: 8383 9454 1391
Size: 1071 2174
Fragments arranged by size:
2175 2174 1858 1071 961 742 517 399
255 85
BspEI T'CCGG_A
Cuts at: 9900 9900
Size: 10237
BspHI T'CATG_A
Cuts at: 4800 8126 9856 4800
Size: 3326 1730 5181
Fragments arranged by size:
5181 3326 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2117 2941 7404 7592 844
Size: 1273 824 4463 188 3489
Fragments arranged by size:
4463 3489 1273 824 188
BsrBI CCG'CTC
Cuts at: 1394 1823 8124 1394
Size: 429 6301 3507
Fragments arranged by size:
6301 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 8732 8906 65
Size: 1183 7484 174 1396
Fragments arranged by size:
7484 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 8881 9068 1700
Size: 110 7071 187 2869
Fragments arranged by size:
7071 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3853 4581 6280 96
Size: 3757 728 1699 4053
Fragments arranged by size:
4053 3757 1699 728
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10237
BssSI C'ACGA_G
Cuts at: 8297 9309 8297
Size: 1012 9225
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3242 5432 794
Size: 2448 2190 5599
Fragments arranged by size:
5599 2448 2190
BstBI TT'CG_AA
Cuts at: 7408 9041 7408
Size: 1633 8604
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 10237
BstXI CCAn_nnnn'nTGG
Cuts at: 5450 5492 5713 5450
Size: 42 221 9974
Fragments arranged by size:
9974 221 42
BstYI r'GATC_y
Cuts at: 1972 2647 3791 4028 4717 7222 7567 7934 8319
Size: 675 1144 237 689 2505 345 367 385
Cuts at: 8319 8336 9788 9874 9886 10232 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2505 1977 1452 1144 689 675 385 367
346 345 237 86 17 12
BstZ17I GTA'TAC
Cuts at: 7273 7273
Size: 10237
BtgI C'CryG_G
Cuts at: 513 1065 2004 2022 513
Size: 552 939 18 8728
Fragments arranged by size:
8728 939 552 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9498
Fragments arranged by size:
9498 683 56
BtsI GCAGTG_nn'
Cuts at: 4596 5433 5497 7753 8547 8567 4596
Size: 837 64 2256 794 20 6266
Fragments arranged by size:
6266 2256 837 794 64 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10237
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5391 5426 529
Size: 35 4827 35 5340
Fragments arranged by size:
5340 4827 35 35
DraI TTT'AAA
Cuts at: 2445 2817 4284 4693 5341 5728 6441 6942 7541
Size: 372 1467 409 648 387 713 501 599
Cuts at: 7541 2445
Size: 5141
Fragments arranged by size:
5141 1467 713 648 599 501 409 387
372
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2900 9244 809
Size: 507 1584 6344 1802
Fragments arranged by size:
6344 1802 1584 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2019 7684 8573
Size: 54 1425 50 275 18 189 5665 889
Cuts at: 8573 10202 8
Size: 1629 43
Fragments arranged by size:
5665 1629 1425 889 275 189 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7684 1537
Size: 275 5872 4090
Fragments arranged by size:
5872 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2838 3313 5116 5254 5358 5485 7213 7307
Size: 1485 475 1803 138 104 127 1728 94
Cuts at: 7307 7359 8167 1353
Size: 52 808 3423
Fragments arranged by size:
3423 1803 1728 1485 808 475 138 127
104 94 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2031 2988 8824 9198 9344 387
Size: 1064 138 442 957 5836 374 146 1280
Fragments arranged by size:
5836 1280 1064 957 442 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2784 3189 3437 3768 4062 4188 4298
Size: 393 1152 405 248 331 294 126 110
Cuts at: 4298 4845 4985 5084 5172 5309 5895 6441 7140
Size: 547 140 99 88 137 586 546 699
Cuts at: 7140 8300 8897 9684 9952 1239
Size: 1160 597 787 268 1524
Fragments arranged by size:
1524 1160 1152 787 699 597 586 547
546 405 393 331 294 268 248 140
137 126 110 99 88
EcoNI CCTnn'n_nnAGG
Cuts at: 2586 3653 2586
Size: 1067 9170
EcoO109I rG'GnC_Cy
Cuts at: 2014 2014
Size: 10237
EcoRI G'AATT_C
Cuts at: 4762 6497 7198 7482 7546 7924 4762
Size: 1735 701 284 64 378 7075
Fragments arranged by size:
7075 1735 701 378 284 64
EcoRV GAT'ATC
Cuts at: 6181 7320 6181
Size: 1139 9098
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2487 3615 3934 4107 4528 5177 2487
Size: 1128 319 173 421 649 7547
Fragments arranged by size:
7547 1128 649 421 319 173
FspI TGC'GCA
Cuts at: 8743 8743
Size: 10237
HaeII r_GCGC'y
Cuts at: 1678 4195 9384 1678
Size: 2517 5189 2531
Fragments arranged by size:
5189 2531 2517
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2087 2119 5985 6017 6956
Size: 32 135 32 515 32 3866 32 939
Cuts at: 6956 6988 7341 7373 8878 8910 8952 8984 1373
Size: 32 353 32 1505 32 42 32 2626
Fragments arranged by size:
3866 2626 1505 939 515 353 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2281 2537 2893 3783 3869 6162
Size: 988 281 343 256 356 890 86 2293
Cuts at: 6162 7581 7827 669
Size: 1419 246 3079
Fragments arranged by size:
3079 2293 1419 988 890 356 343 281
256 246 86
HindIII A'AGCT_T
Cuts at: 748 2659 3722 4137 748
Size: 1911 1063 415 6848
Fragments arranged by size:
6848 1911 1063 415
HpaI GTT'AAC
Cuts at: 6162 7827 6162
Size: 1665 8572
KpnI G_GTAC'C
Cuts at: 7562 7562
Size: 10237
MfeI C'AATT_G
Cuts at: 2403 4118 7836 2403
Size: 1715 3718 4804
Fragments arranged by size:
4804 3718 1715
MluI A'CGCG_T
Cuts at: 10113 10113
Size: 10237
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5848 7019 7250 9351 9535 1305
Size: 4543 1171 231 2101 184 2007
Fragments arranged by size:
4543 2101 2007 1171 231 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2021 10
Size: 54 1425 343 189 8226
Fragments arranged by size:
8226 1425 343 189 54
MslI CAynn'nnrTG
Cuts at: 518 1166 8195 8554 8713 518
Size: 648 7029 359 159 2042
Fragments arranged by size:
7029 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2036 4424 7136 7526 7653 8330 9478 9723
Size: 430 2388 2712 390 127 677 1148 245
Cuts at: 9723 1606
Size: 2120
Fragments arranged by size:
2712 2388 2120 1148 677 430 390 245
127
NcoI C'CATG_G
Cuts at: 513 1065 2004 2022 513
Size: 552 939 18 8728
Fragments arranged by size:
8728 939 552 18
NdeI CA'TA_TG
Cuts at: 387 2803 387
Size: 2416 7821
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10237
NotI GC'GGCC_GC
Cuts at: 7684 7684
Size: 10237
NspI r_CATG'y
Cuts at: 1132 1229 4622 5161 6973 7595 9140 1132
Size: 97 3393 539 1812 622 1545 2229
Fragments arranged by size:
3393 2229 1812 1545 622 539 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 10237
PciI A'CATG_T
Cuts at: 4618 6969 9136 4618
Size: 2351 2167 5719
Fragments arranged by size:
5719 2351 2167
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 10237
PmeI GTTT'AAAC
Cuts at: 7541 7541
Size: 10237
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8306 8338 9819 9851 8306
Size: 32 1481 32 8692
Fragments arranged by size:
8692 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2014 2014
Size: 10237
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10237
PsiI TTA'TAA
Cuts at: 4256 5393 7807 4256
Size: 1137 2414 6686
Fragments arranged by size:
6686 2414 1137
PstI C_TGCA'G
Cuts at: 830 2035 5428 6907 7141 7589 830
Size: 1205 3393 1479 234 448 3478
Fragments arranged by size:
3478 3393 1479 1205 448 234
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10237
PvuII CAG'CTG
Cuts at: 2036 4424 7136 7526 2036
Size: 2388 2712 390 4747
Fragments arranged by size:
4747 2712 2388 390
SalI G'TCGA_C
Cuts at: 1936 2891 7579 1936
Size: 955 4688 4594
Fragments arranged by size:
4688 4594 955
SapI GCTCTTCn'nnn_
Cuts at: 5254 5254
Size: 10237
SbfI CC_TGCA'GG
Cuts at: 7589 7589
Size: 10237
ScaI AGT'ACT
Cuts at: 2470 3994 4932 6070 8485 10197 2470
Size: 1524 938 1138 2415 1712 2510
Fragments arranged by size:
2510 2415 1712 1524 1138 938
SfcI C'TryA_G
Cuts at: 826 1045 2031 2437 3819 5424 6903 7137 7585
Size: 219 986 406 1382 1605 1479 234 448
Cuts at: 7585 8720 9401 9592 826
Size: 1135 681 191 1471
Fragments arranged by size:
1605 1479 1471 1382 1135 986 681 448
406 234 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10237
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10237
SmaI CCC'GGG
Cuts at: 1872 7564 1872
Size: 5692 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4186 4847 6398 6536 7085 8341
Size: 197 926 2243 661 1551 138 549 1256
Cuts at: 8341 9242 9504 820
Size: 901 262 1553
Fragments arranged by size:
2243 1553 1551 1256 926 901 661 549
262 197 138
SnaBI TAC'GTA
Cuts at: 493 8028 493
Size: 7535 2702
SpeI A'CTAG_T
Cuts at: 152 6286 152
Size: 6134 4103
SphI G_CATG'C
Cuts at: 1132 7595 1132
Size: 6463 3774
SspI AAT'ATT
Cuts at: 5 52 2129 2188 2380 4892 6720 8161 5
Size: 47 2077 59 192 2512 1828 1441 2081
Fragments arranged by size:
2512 2081 2077 1828 1441 192 59 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 10237
StyI C'CwwG_G
Cuts at: 513 1065 2004 2022 2667 5712 7676 513
Size: 552 939 18 645 3045 1964 3074
Fragments arranged by size:
3074 3045 1964 939 645 552 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2230 4629 8264 8281 8434 8619 9064
Size: 1255 650 2399 3635 17 153 185 445
Cuts at: 9064 325
Size: 1498
Fragments arranged by size:
3635 2399 1498 1255 650 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2468 3853 3992
Size: 274 80 33 51 1443 491 1385 139
Cuts at: 3992 4581 4930 6068 6280 8483 10195 96
Size: 589 349 1138 212 2203 1712 138
Fragments arranged by size:
2203 1712 1443 1385 1138 589 491 349
274 212 139 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4625 4657 4844 4876 6205 6237 10197 10229 4625
Size: 32 187 32 1329 32 3960 32 4633
Fragments arranged by size:
4633 3960 1329 187 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 10237
XbaI T'CTAG_A
Cuts at: 2747 3193 4199 7573 2747
Size: 446 1006 3374 5411
Fragments arranged by size:
5411 3374 1006 446
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10237
XmaI C'CCGG_G
Cuts at: 1870 7562 1870
Size: 5692 4545
XmnI GAAnn'nnTTC
Cuts at: 2209 2517 7482 8366 2209
Size: 308 4965 884 4080
Fragments arranged by size:
4965 4080 884 308
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8045 276
Size: 53 83 186 936 6511 2468
Fragments arranged by size:
6511 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI Bsu36I DraIII EcoICRI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI