Restriction map for
pFN21AB7339
(Circular) (Six-base) MAPSORT of: pFN21AB7339.seq Check: 7774 from: 1 to: 7204
pFN21AB7339 7204 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3552 3937 4129 4309 3552
Size: 385 192 180 6447
Fragments arranged by size:
6447 385 192 180
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2013 3893 5014 278
Size: 53 83 186 936 477 1880 1121 2468
Fragments arranged by size:
2468 1880 1121 936 477 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4547 1937
Size: 2610 4594
Acc65I G'GTAC_C
Cuts at: 3912 4525 3912
Size: 613 6591
AclI AA'CG_TT
Cuts at: 1563 3654 5331 5704 1563
Size: 2091 1677 373 3063
Fragments arranged by size:
3063 2091 1677 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2159 2165 2588 2735 2897 3890 4277 5267
Size: 527 6 423 147 162 993 387 990
Cuts at: 5267 6651 6919 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 993 990 527 423 387 268
162 147 6
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7007
AflIII A'CryG_T
Cuts at: 1203 6103 7080 1203
Size: 4900 977 1327
Fragments arranged by size:
4900 1327 977
AgeI A'CCGG_T
Cuts at: 6035 6035
Size: 7204
AhdI GACnn_n'nnGTC
Cuts at: 2116 2599 2775 5933 2116
Size: 483 176 3158 3387
Fragments arranged by size:
3387 3158 483 176
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7204
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7204
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3374 3406 3779 3811 3374
Size: 32 373 32 6767
Fragments arranged by size:
6767 373 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2071 2377 3544 3952 4007 4357 4947 6519 2071
Size: 306 1167 408 55 350 590 1572 2756
Fragments arranged by size:
2756 1572 1167 590 408 350 306 55
ApaI G_GGCC'C
Cuts at: 3300 3866 3300
Size: 566 6638
ApaLI G'TGCA_C
Cuts at: 2657 5261 6417 2657
Size: 2604 1156 3444
Fragments arranged by size:
3444 2604 1156
ApoI r'AATT_y
Cuts at: 1493 2279 3929 4513 4739 4891 7000 1493
Size: 786 1650 584 226 152 2109 1697
Fragments arranged by size:
2109 1697 1650 786 584 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5758 160
Size: 872 4726 1606
Fragments arranged by size:
4726 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7204
AvaI C'yCGr_G
Cuts at: 1870 1943 2043 2106 2885 2927 3087 3268 3393
Size: 73 100 63 779 42 160 181 125
Cuts at: 3393 4040 4479 4529 1870
Size: 647 439 50 4545
Fragments arranged by size:
4545 779 647 439 181 160 125 100
73 63 50 42
BamHI G'GATC_C
Cuts at: 2988 3495 4534 4901 2988
Size: 507 1039 367 5291
Fragments arranged by size:
5291 1039 507 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3912 4525 5980 618
Size: 325 217 627 2125 613 1455 1842
Fragments arranged by size:
2125 1842 1455 627 613 325 217
BanII G_rGCy'C
Cuts at: 1391 2340 2928 3269 3300 3857 3866 4040 4330
Size: 949 588 341 31 557 9 174 290
Cuts at: 4330 4523 1391
Size: 193 4072
Fragments arranged by size:
4072 949 588 557 341 290 193 174
31 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6233
BbvCI CC'TCA_GC
Cuts at: 4471 4471
Size: 7204
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5395 5429 1903
Size: 34 3458 34 3678
Fragments arranged by size:
3678 3458 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2570 2836 5096 6306 2570
Size: 266 2260 1210 3468
Fragments arranged by size:
3468 2260 1210 266
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7204
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5815 136
Size: 107 122 71 631 605 4143 1525
Fragments arranged by size:
4143 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7199 7199
Size: 7204
BlpI GC'TnA_GC
Cuts at: 3263 4621 3263
Size: 1358 5846
Bme1580I G_kGCm'C
Cuts at: 1790 2661 3300 3460 3866 5265 6421 1790
Size: 871 639 160 406 1399 1156 2573
Fragments arranged by size:
2573 1399 1156 871 639 406 160
BmgBI CAC'GTC
Cuts at: 1318 3154 1318
Size: 1836 5368
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4211 4935 5893
Size: 416 53 83 399 24 2794 724 958
Cuts at: 5893 442
Size: 1753
Fragments arranged by size:
2794 1753 958 724 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7204
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2285 2324 2354 2959 4424 5864 1239
Size: 1046 39 30 605 1465 1440 2579
Fragments arranged by size:
2579 1465 1440 1046 605 39 30
Bpu10I CC'TnA_GC
Cuts at: 4471 4471
Size: 7204
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5329 6194 6492 5329
Size: 865 298 6041
Fragments arranged by size:
6041 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2031 3041 3084 3722 4356 5867 882
Size: 617 532 1010 43 638 634 1511 2219
Fragments arranged by size:
2219 1511 1010 638 634 617 532 43
BsaAI yAC'GTr
Cuts at: 493 1204 2085 3633 4995 493
Size: 711 881 1548 1362 2702
Fragments arranged by size:
2702 1548 1362 881 711
BsaBI GATnn'nnATC
Cuts at: 7198 7198
Size: 7204
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2010 2509 3890 3968
Size: 53 83 186 936 477 499 1381 78
Cuts at: 3968 5011 5393 6028 275
Size: 1043 382 635 1451
Fragments arranged by size:
1451 1381 1043 936 635 499 477 382
186 83 78 53
BsaWI w'CCGG_w
Cuts at: 2545 3282 3492 3582 5637 6035 6309 6456 6867
Size: 737 210 90 2055 398 274 147 411
Cuts at: 6867 2545
Size: 2882
Fragments arranged by size:
2882 2055 737 411 398 274 210 147
90
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3172 3202 3374 3404 4305
Size: 30 165 30 1769 30 172 30 901
Cuts at: 4305 4335 1178
Size: 30 4047
Fragments arranged by size:
4047 1769 901 172 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2936 3075 3177 4430 4433 1186
Size: 1750 139 102 1253 3 3957
Fragments arranged by size:
3957 1750 1253 139 102 3
BseYI C'CCAG_C
Cuts at: 2071 2447 2792 2829 3058 4102 4487 6407 6903
Size: 376 345 37 229 1044 385 1920 496
Cuts at: 6903 2071
Size: 2372
Fragments arranged by size:
2372 1920 1044 496 385 376 345 229
37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4157 4337 4429 1163
Size: 573 2421 180 92 3938
Fragments arranged by size:
3938 2421 573 180 92
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3510 4049 4654 5415 6443 1540
Size: 275 186 1509 539 605 761 1028 2301
Fragments arranged by size:
2301 1509 1028 761 605 539 275 186
BsiHKAI G_wGCw'C
Cuts at: 2340 2370 2661 3857 4065 4118 4125 5265 5350
Size: 30 291 1196 208 53 7 1140 85
Cuts at: 5350 6421 2340
Size: 1071 3123
Fragments arranged by size:
3123 1196 1140 1071 291 208 85 53
30 7
BsmI GAATG_Cn'
Cuts at: 4715 4808 4715
Size: 93 7111
BsmBI CGTCTCn'nnnn_
Cuts at: 3881 3881
Size: 7204
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2340 2370 2661 2928 3269 3300 3460
Size: 399 550 30 291 267 341 31 160
Cuts at: 3460 3857 3866 4040 4065 4118 4125 4330 4523
Size: 397 9 174 25 53 7 205 193
Cuts at: 4523 5265 5350 6421 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 550 399 397 341 291
267 205 193 174 160 85 53 31
30 25 9 7
BspEI T'CCGG_A
Cuts at: 2545 3492 6867 2545
Size: 947 3375 2882
Fragments arranged by size:
3375 2882 947
BspHI T'CATG_A
Cuts at: 2682 5093 6823 2682
Size: 2411 1730 3063
Fragments arranged by size:
3063 2411 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2067 3552 3937 4129 4309 4559 844
Size: 1223 1485 385 192 180 250 3489
Fragments arranged by size:
3489 1485 1223 385 250 192 180
BsrBI CCG'CTC
Cuts at: 1394 1823 2647 2973 3071 5091 1394
Size: 429 824 326 98 2020 3507
Fragments arranged by size:
3507 2020 824 429 326 98
BsrDI GCAATG_nn'
Cuts at: 65 1248 5699 5873 65
Size: 1183 4451 174 1396
Fragments arranged by size:
4451 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2211 5848 6035 1700
Size: 110 401 3637 187 2869
Fragments arranged by size:
3637 2869 401 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7204
BssHII G'CGCG_C
Cuts at: 1925 4086 1925
Size: 2161 5043
BssSI C'ACGA_G
Cuts at: 5264 6276 5264
Size: 1012 6192
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4315 794
Size: 3521 3683
BstBI TT'CG_AA
Cuts at: 6008 6008
Size: 7204
BstEII G'GTnAC_C
Cuts at: 1184 4141 4198 1184
Size: 2957 57 4190
Fragments arranged by size:
4190 2957 57
BstXI CCAn_nnnn'nTGG
Cuts at: 2227 2626 2983 2227
Size: 399 357 6448
Fragments arranged by size:
6448 399 357
BstYI r'GATC_y
Cuts at: 1972 2268 2860 2960 2988 3495 3804 4242 4534
Size: 296 592 100 28 507 309 438 292
Cuts at: 4534 4901 5286 5303 6755 6841 6853 7199 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 592 507 438 385 367 346
309 296 292 100 86 28 17 12
Bsu36I CC'TnA_GG
Cuts at: 3095 3095
Size: 7204
BtgI C'CryG_G
Cuts at: 513 1065 2004 2236 4269 513
Size: 552 939 232 2033 3448
Fragments arranged by size:
3448 2033 939 552 232
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3733 487
Size: 683 56 2507 3958
Fragments arranged by size:
3958 2507 683 56
BtsI GCAGTG_nn'
Cuts at: 3959 4417 4429 4720 5514 5534 3959
Size: 458 12 291 794 20 5629
Fragments arranged by size:
5629 794 458 291 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7204
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7169
DraI TTT'AAA
Cuts at: 4508 4508
Size: 7204
DraIII CAC_nnn'GTG
Cuts at: 4075 4075
Size: 7204
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3973 6211 809
Size: 507 2657 2238 1802
Fragments arranged by size:
2657 2238 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2502 2616 2628
Size: 54 1425 50 275 18 672 114 12
Cuts at: 2628 3507 4046 4651 5540 7169 8
Size: 879 539 605 889 1629 43
Fragments arranged by size:
1629 1425 889 879 672 605 539 275
114 54 50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 3507 4046 4651 1537
Size: 275 1695 539 605 4090
Fragments arranged by size:
4090 1695 605 539 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2681 3437 4070 5134 1353
Size: 1328 756 633 1064 3423
Fragments arranged by size:
3423 1328 1064 756 633
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2968 3857 5791 6165 6311 387
Size: 1064 138 1379 889 1934 374 146 1280
Fragments arranged by size:
1934 1379 1280 1064 889 374 146 138
EcoICRI GAG'CTC
Cuts at: 2338 3855 2338
Size: 1517 5687
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2159 2165 2285 2324 2354 2588 2735
Size: 393 527 6 120 39 30 234 147
Cuts at: 2735 2897 2959 3890 4277 4424 5267 5864 6651
Size: 162 62 931 387 147 843 597 787
Cuts at: 6651 6919 1239
Size: 268 1524
Fragments arranged by size:
1524 931 843 787 597 527 393 387
268 234 162 147 147 120 62 39
30 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2183 2183
Size: 7204
EcoO109I rG'GnC_Cy
Cuts at: 2125 2894 3233 3297 3862 4130 4212 2125
Size: 769 339 64 565 268 82 5117
Fragments arranged by size:
5117 769 565 339 268 82 64
EcoRI G'AATT_C
Cuts at: 2279 4513 4891 2279
Size: 2234 378 4592
Fragments arranged by size:
4592 2234 378
FspI TGC'GCA
Cuts at: 5710 5710
Size: 7204
HaeII r_GCGC'y
Cuts at: 1678 2799 6351 1678
Size: 1121 3552 2531
Fragments arranged by size:
3552 2531 1121
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2102 2134 2156 2188 2415
Size: 32 135 32 530 32 22 32 227
Cuts at: 2415 2447 2585 2617 2761 2793 2797 2829 2838
Size: 32 138 32 144 32 4 32 9
Cuts at: 2838 2845 2870 2877 4411 4443 5845 5877 5919
Size: 7 25 7 1534 32 1402 32 42
Cuts at: 5919 5951 1373
Size: 32 2626
Fragments arranged by size:
2626 1534 1402 530 227 144 138 135
42 32 32 32 32 32 32 32
32 32 32 32 25 22 9 7
7 4
HincII GTy'rAC
Cuts at: 669 1657 1938 4548 4794 669
Size: 988 281 2610 246 3079
Fragments arranged by size:
3079 2610 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7204
HpaI GTT'AAC
Cuts at: 4794 4794
Size: 7204
KpnI G_GTAC'C
Cuts at: 3916 4529 3916
Size: 613 6591
MfeI C'AATT_G
Cuts at: 4803 4803
Size: 7204
MluI A'CGCG_T
Cuts at: 7080 7080
Size: 7204
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4261 6318 6502 1305
Size: 2956 2057 184 2007
Fragments arranged by size:
2956 2057 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2504 10
Size: 54 1425 343 672 4710
Fragments arranged by size:
4710 1425 672 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2804 4031 5162 5521 5680 518
Size: 648 1638 1227 1131 359 159 2042
Fragments arranged by size:
2042 1638 1227 1131 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2068 2558 3262 4019 4345 4620 5297 6445
Size: 462 490 704 757 326 275 677 1148
Cuts at: 6445 6690 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 757 704 677 490 462 326
275 245
NaeI GCC'GGC
Cuts at: 2213 2213
Size: 7204
NcoI C'CATG_G
Cuts at: 513 1065 2004 2236 513
Size: 552 939 232 5481
Fragments arranged by size:
5481 939 552 232
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7204
NgoMIV G'CCGG_C
Cuts at: 2211 2211
Size: 7204
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7204
NotI GC'GGCC_GC
Cuts at: 4651 4651
Size: 7204
NspI r_CATG'y
Cuts at: 1132 1229 4562 6107 1132
Size: 97 3333 1545 2229
Fragments arranged by size:
3333 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2605 1348
Size: 1257 5947
PciI A'CATG_T
Cuts at: 6103 6103
Size: 7204
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2377 1108
Size: 1269 5935
PfoI T'CCnGG_A
Cuts at: 2301 2856 3807 2301
Size: 555 951 5698
Fragments arranged by size:
5698 951 555
PmeI GTTT'AAAC
Cuts at: 4508 4508
Size: 7204
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3360 3374 3392 3406 5273 5305 6786 6818 3360
Size: 14 18 14 1867 32 1481 32 3746
Fragments arranged by size:
3746 1867 1481 32 32 18 14 14
PpuMI rG'GwC_Cy
Cuts at: 2125 2894 3233 4130 4212 2125
Size: 769 339 897 82 5117
Fragments arranged by size:
5117 897 769 339 82
PshAI GACnn'nnGTC
Cuts at: 1613 3006 3231 1613
Size: 1393 225 5586
Fragments arranged by size:
5586 1393 225
PsiI TTA'TAA
Cuts at: 4774 4774
Size: 7204
PspOMI G'GGCC_C
Cuts at: 3296 3862 3296
Size: 566 6638
PstI C_TGCA'G
Cuts at: 830 3175 4556 830
Size: 2345 1381 3478
Fragments arranged by size:
3478 2345 1381
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7204
PvuII CAG'CTG
Cuts at: 2558 3262 4345 2558
Size: 704 1083 5417
Fragments arranged by size:
5417 1083 704
SacI G_AGCT'C
Cuts at: 2340 3857 2340
Size: 1517 5687
SalI G'TCGA_C
Cuts at: 1936 4546 1936
Size: 2610 4594
SanDI GG'GwC_CC
Cuts at: 4130 4212 4130
Size: 82 7122
SapI GCTCTTCn'nnn_
Cuts at: 4070 4070
Size: 7204
SbfI CC_TGCA'GG
Cuts at: 4556 4556
Size: 7204
ScaI AGT'ACT
Cuts at: 5452 7164 5452
Size: 1712 5492
SexAI A'CCwGG_T
Cuts at: 2127 2127
Size: 7204
SfcI C'TryA_G
Cuts at: 826 1045 2063 2636 3077 3171 4349 4552 5687
Size: 219 1018 573 441 94 1178 203 1135
Cuts at: 5687 6368 6559 826
Size: 681 191 1471
Fragments arranged by size:
1471 1178 1135 1018 681 573 441 219
203 191 94
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7204
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7204
SmaI CCC'GGG
Cuts at: 1872 2045 2887 3270 3395 4042 4531 1872
Size: 173 842 383 125 647 489 4545
Fragments arranged by size:
4545 842 647 489 383 173 125
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2106 3087 5308 6209 6471 820
Size: 197 926 163 981 2221 901 262 1553
Fragments arranged by size:
2221 1553 981 926 901 262 197 163
SnaBI TAC'GTA
Cuts at: 493 4995 493
Size: 4502 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7204
SphI G_CATG'C
Cuts at: 1132 4562 1132
Size: 3430 3774
SspI AAT'ATT
Cuts at: 5 52 5128 5
Size: 47 5076 2081
Fragments arranged by size:
5076 2081 47
StuI AGG'CCT
Cuts at: 1517 2850 4298 4500 1517
Size: 1333 1448 202 4221
Fragments arranged by size:
4221 1448 1333 202
StyI C'CwwG_G
Cuts at: 513 1065 2004 2236 3531 3706 4643 513
Size: 552 939 232 1295 175 937 3074
Fragments arranged by size:
3074 1295 939 937 552 232 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2063 2981 3625 4268 5231 5248 5401
Size: 1255 483 918 644 643 963 17 153
Cuts at: 5401 5586 6031 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 963 918 644 643 483 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3639 5450 7162
Size: 274 80 33 51 1443 1662 1811 1712
Cuts at: 7162 96
Size: 138
Fragments arranged by size:
1811 1712 1662 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3172 3204 4227 4259 4305 4337 7164 7196 3172
Size: 32 1023 32 46 32 2827 32 3180
Fragments arranged by size:
3180 2827 1023 46 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7204
XbaI T'CTAG_A
Cuts at: 4540 4540
Size: 7204
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 7204
XhoI C'TCGA_G
Cuts at: 1943 2106 3087 1943
Size: 163 981 6060
Fragments arranged by size:
6060 981 163
XmaI C'CCGG_G
Cuts at: 1870 2043 2885 3268 3393 4040 4529 1870
Size: 173 842 383 125 647 489 4545
Fragments arranged by size:
4545 842 647 489 383 173 125
XmnI GAAnn'nnTTC
Cuts at: 3929 5333 3929
Size: 1404 5800
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2011 3891 5012 276
Size: 53 83 186 936 477 1880 1121 2468
Fragments arranged by size:
2468 1880 1121 936 477 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspXI PsrI RsrII SacII SfiI
SfoI SrfI SwaI