Restriction map for
pFN21AB7338
(Circular) (Six-base) MAPSORT of: pFN21AB7338.seq Check: 4146 from: 1 to: 7894
pFN21AB7338 7894 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2522 4351 2522
Size: 1829 6065
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5704 278
Size: 53 83 186 936 4168 2468
Fragments arranged by size:
4168 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5237 1937
Size: 3300 4594
Acc65I G'GTAC_C
Cuts at: 5215 5215
Size: 7894
AclI AA'CG_TT
Cuts at: 1563 6021 6394 1563
Size: 4458 373 3063
Fragments arranged by size:
4458 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2521 3100 5957 7341 7609 1632
Size: 889 579 2857 1384 268 1917
Fragments arranged by size:
2857 1917 1384 889 579 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7697
AflIII A'CryG_T
Cuts at: 1203 4729 6793 7770 1203
Size: 3526 2064 977 1327
Fragments arranged by size:
3526 2064 1327 977
AgeI A'CCGG_T
Cuts at: 3457 6725 3457
Size: 3268 4626
AhdI GACnn_n'nnGTC
Cuts at: 6623 6623
Size: 7894
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7894
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7894
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5061 5093 5061
Size: 32 7862
AlwNI CAG_nnn'CTG
Cuts at: 2819 3484 5637 7209 2819
Size: 665 2153 1572 3504
Fragments arranged by size:
3504 2153 1572 665
ApaI G_GGCC'C
Cuts at: 4870 4870
Size: 7894
ApaLI G'TGCA_C
Cuts at: 5951 7107 5951
Size: 1156 6738
ApoI r'AATT_y
Cuts at: 1493 2865 2884 2935 3050 3779 4556 4739 5203
Size: 1372 19 51 115 729 777 183 464
Cuts at: 5203 5429 5581 7690 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1372 777 729 464 226 183
152 115 51 19
AseI AT'TA_AT
Cuts at: 160 1032 6448 160
Size: 872 5416 1606
Fragments arranged by size:
5416 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7894
AvaI C'yCGr_G
Cuts at: 1870 1943 2012 2031 4548 5219 1870
Size: 73 69 19 2517 671 4545
Fragments arranged by size:
4545 2517 671 73 69 19
BamHI G'GATC_C
Cuts at: 5224 5591 5224
Size: 367 7527
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2044 2233 2510 3028 3140
Size: 325 217 627 257 189 277 518 112
Cuts at: 3140 5215 6670 618
Size: 2075 1455 1842
Fragments arranged by size:
2075 1842 1455 627 518 325 277 257
217 189 112
BanII G_rGCy'C
Cuts at: 1391 3107 4437 4674 4757 4870 5213 1391
Size: 1716 1330 237 83 113 343 4072
Fragments arranged by size:
4072 1716 1330 343 237 113 83
BbeI G_GCGC'C
Cuts at: 2048 2237 2048
Size: 189 7705
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3697 3939 928
Size: 971 1798 242 4883
Fragments arranged by size:
4883 1798 971 242
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2155 2189 4258 4292 6085 6119 1903
Size: 34 218 34 2069 34 1793 34 3678
Fragments arranged by size:
3678 2069 1793 218 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2801 3197 5786 6996 2801
Size: 396 2589 1210 3699
Fragments arranged by size:
3699 2589 1210 396
BclI T'GATC_A
Cuts at: 1548 2688 1548
Size: 1140 6754
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2114 2163 6505
Size: 107 122 71 631 605 442 49 4342
Cuts at: 6505 136
Size: 1525
Fragments arranged by size:
4342 1525 631 605 442 122 107 71
49
BglII A'GATC_T
Cuts at: 7889 7889
Size: 7894
BlpI GC'TnA_GC
Cuts at: 4673 5311 4673
Size: 638 7256
Bme1580I G_kGCm'C
Cuts at: 1790 3031 4870 5955 7111 1790
Size: 1241 1839 1085 1156 2573
Fragments arranged by size:
2573 1839 1241 1156 1085
BmgBI CAC'GTC
Cuts at: 1318 2131 4247 1318
Size: 813 2116 4965
Fragments arranged by size:
4965 2116 813
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2498 2844 4426
Size: 416 53 83 399 24 1081 346 1582
Cuts at: 4426 5625 6583 442
Size: 1199 958 1753
Fragments arranged by size:
1753 1582 1199 1081 958 416 399 346
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7894
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2491 2763 4544 4865 6554 1239
Size: 1252 272 1781 321 1689 2579
Fragments arranged by size:
2579 1781 1689 1252 321 272
Bpu10I CC'TnA_GC
Cuts at: 4668 5095 4668
Size: 427 7467
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4271 6019 6884 7182 4271
Size: 1748 865 298 4983
Fragments arranged by size:
4983 1748 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2634 3114 3180 6557 882
Size: 617 1135 480 66 3377 2219
Fragments arranged by size:
3377 2219 1135 617 480 66
BsaAI yAC'GTr
Cuts at: 493 1204 5685 493
Size: 711 4481 2702
Fragments arranged by size:
4481 2702 711
BsaBI GATnn'nnATC
Cuts at: 3687 3981 4005 7888 3687
Size: 294 24 3883 3693
Fragments arranged by size:
3883 3693 294 24
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2045 2205 2234 5701
Size: 53 83 186 936 512 160 29 3467
Cuts at: 5701 6083 6718 275
Size: 382 635 1451
Fragments arranged by size:
3467 1451 936 635 512 382 186 160
83 53 29
BsaWI w'CCGG_w
Cuts at: 2584 3347 3457 6327 6725 6999 7146 7557 2584
Size: 763 110 2870 398 274 147 411 2921
Fragments arranged by size:
2921 2870 763 411 398 274 147 110
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2406 2436 3148 3178 1178
Size: 30 165 30 1003 30 712 30 5894
Fragments arranged by size:
5894 1003 712 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2889 3101 3384 4389 4445 4460 4463 4842
Size: 1703 212 283 1005 56 15 3 379
Cuts at: 4842 5127 1186
Size: 285 3953
Fragments arranged by size:
3953 1703 1005 379 285 283 212 56
15 3
BseYI C'CCAG_C
Cuts at: 2083 2822 3477 7097 7593 2083
Size: 739 655 3620 496 2384
Fragments arranged by size:
3620 2384 739 655 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2341 3473 4469 4827 1163
Size: 573 605 1132 996 358 4230
Fragments arranged by size:
4230 1132 996 605 573 358
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2079 5344 6105 7133 1540
Size: 275 186 78 3265 761 1028 2301
Fragments arranged by size:
3265 2301 1028 761 275 186 78
BsiHKAI G_wGCw'C
Cuts at: 2458 2881 3107 3264 4674 5955 6040 7111 2458
Size: 423 226 157 1410 1281 85 1071 3241
Fragments arranged by size:
3241 1410 1281 1071 423 226 157 85
BsmI GAATG_Cn'
Cuts at: 5405 5498 5405
Size: 93 7801
BsmBI CGTCTCn'nnnn_
Cuts at: 3140 3140
Size: 7894
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2458 2881 3031 3107 3264 4437 4674
Size: 399 668 423 150 76 157 1173 237
Cuts at: 4674 4757 4870 5213 5955 6040 7111 1391
Size: 83 113 343 742 85 1071 2174
Fragments arranged by size:
2174 1173 1071 742 668 423 399 343
237 157 150 113 85 83 76
BspEI T'CCGG_A
Cuts at: 2584 3347 7557 2584
Size: 763 4210 2921
Fragments arranged by size:
4210 2921 763
BspHI T'CATG_A
Cuts at: 5783 7513 5783
Size: 1730 6164
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2522 4351 5249 844
Size: 1678 1829 898 3489
Fragments arranged by size:
3489 1829 1678 898
BsrBI CCG'CTC
Cuts at: 1394 1823 5781 1394
Size: 429 3958 3507
Fragments arranged by size:
3958 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2581 2712 3213 3597 6389 6563 65
Size: 1183 1333 131 501 384 2792 174 1396
Fragments arranged by size:
2792 1396 1333 1183 501 384 174 131
BsrFI r'CCGG_y
Cuts at: 1700 1810 3457 6538 6725 1700
Size: 110 1647 3081 187 2869
Fragments arranged by size:
3081 2869 1647 187 110
BsrGI T'GTAC_A
Cuts at: 96 2148 4726 4775 96
Size: 2052 2578 49 3215
Fragments arranged by size:
3215 2578 2052 49
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7894
BssSI C'ACGA_G
Cuts at: 5954 6966 5954
Size: 1012 6882
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7894
BstBI TT'CG_AA
Cuts at: 6698 6698
Size: 7894
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7894
BstXI CCAn_nnnn'nTGG
Cuts at: 4174 4174
Size: 7894
BstYI r'GATC_y
Cuts at: 1972 3129 5224 5591 5976 5993 7445 7531 7543
Size: 1157 2095 367 385 17 1452 86 12
Cuts at: 7543 7889 1972
Size: 346 1977
Fragments arranged by size:
2095 1977 1452 1157 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2741 5139 5187 2741
Size: 2398 48 5448
Fragments arranged by size:
5448 2398 48
BtgI C'CryG_G
Cuts at: 513 1065 2023 2073 2184 2301 513
Size: 552 958 50 111 117 6106
Fragments arranged by size:
6106 958 552 117 111 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3683 487
Size: 683 56 2457 4698
Fragments arranged by size:
4698 2457 683 56
BtsI GCAGTG_nn'
Cuts at: 2324 2691 4347 4607 4815 5410 6204 6224 2324
Size: 367 1656 260 208 595 794 20 3994
Fragments arranged by size:
3994 1656 794 595 367 260 208 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7894
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3305 3340 529
Size: 35 2741 35 5083
Fragments arranged by size:
5083 2741 35 35
DraI TTT'AAA
Cuts at: 3844 5198 3844
Size: 1354 6540
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6901 809
Size: 507 5585 1802
Fragments arranged by size:
5585 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2076 5341 6230
Size: 54 1425 50 275 18 246 3265 889
Cuts at: 6230 7859 8
Size: 1629 43
Fragments arranged by size:
3265 1629 1425 889 275 246 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2076 5341 1537
Size: 275 264 3265 4090
Fragments arranged by size:
4090 3265 275 264
EarI CTCTTCn'nnn_
Cuts at: 1353 3119 3680 4080 4422 4712 5824 1353
Size: 1766 561 400 342 290 1112 3423
Fragments arranged by size:
3423 1766 1112 561 400 342 290
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6481 6855 7001 387
Size: 1064 138 4892 374 146 1280
Fragments arranged by size:
4892 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3105 4672 3105
Size: 1567 6327
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2491 2521 2763 3100 4544 4865 5957
Size: 393 859 30 242 337 1444 321 1092
Cuts at: 5957 6554 7341 7609 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1444 1092 859 787 597 393 337
321 268 242 30
EcoNI CCTnn'n_nnAGG
Cuts at: 3234 3234
Size: 7894
EcoO109I rG'GnC_Cy
Cuts at: 2500 2657 4592 2500
Size: 157 1935 5802
Fragments arranged by size:
5802 1935 157
EcoRI G'AATT_C
Cuts at: 2865 3779 4739 5203 5581 2865
Size: 914 960 464 378 5178
Fragments arranged by size:
5178 960 914 464 378
EcoRV GAT'ATC
Cuts at: 2263 2710 2737 2263
Size: 447 27 7420
Fragments arranged by size:
7420 447 27
FspI TGC'GCA
Cuts at: 2316 6400 2316
Size: 4084 3810
HaeII r_GCGC'y
Cuts at: 1678 2048 2237 2544 4680 7041 1678
Size: 370 189 307 2136 2361 2531
Fragments arranged by size:
2531 2361 2136 370 307 189
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2381 2413 2673 2705 3490
Size: 32 135 32 809 32 260 32 785
Cuts at: 3490 3522 3779 3811 6535 6567 6609 6641 1373
Size: 32 257 32 2724 32 42 32 2626
Fragments arranged by size:
2724 2626 809 785 260 257 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2995 5238 5484 669
Size: 988 281 1057 2243 246 3079
Fragments arranged by size:
3079 2243 1057 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7894
HpaI GTT'AAC
Cuts at: 2995 5484 2995
Size: 2489 5405
KasI G'GCGC_C
Cuts at: 2044 2233 2044
Size: 189 7705
KpnI G_GTAC'C
Cuts at: 5219 5219
Size: 7894
MfeI C'AATT_G
Cuts at: 5493 5493
Size: 7894
MluI A'CGCG_T
Cuts at: 7770 7770
Size: 7894
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4081 4323 4435 7008 7192 1305
Size: 2776 242 112 2573 184 2007
Fragments arranged by size:
2776 2573 2007 242 184 112
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6072
Fragments arranged by size:
6072 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3224 3917 4476 4861 5852 6211 6370
Size: 648 2058 693 559 385 991 359 159
Cuts at: 6370 518
Size: 2042
Fragments arranged by size:
2058 2042 991 693 648 559 385 359
159
MspA1I CmG'CkG
Cuts at: 1606 2025 2050 2075 2154 2303 5167 5310 5987
Size: 419 25 25 79 149 2864 143 677
Cuts at: 5987 7135 7380 1606
Size: 1148 245 2120
Fragments arranged by size:
2864 2120 1148 677 419 245 149 143
79 25 25
NarI GG'CG_CC
Cuts at: 2045 2234 2045
Size: 189 7705
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7342
NdeI CA'TA_TG
Cuts at: 387 4054 387
Size: 3667 4227
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7894
NotI GC'GGCC_GC
Cuts at: 2076 5341 2076
Size: 3265 4629
NspI r_CATG'y
Cuts at: 1132 1229 3338 4733 5252 6797 1132
Size: 97 2109 1395 519 1545 2229
Fragments arranged by size:
2229 2109 1545 1395 519 97
PasI CC'CwG_GG
Cuts at: 1348 4222 1348
Size: 2874 5020
PciI A'CATG_T
Cuts at: 4729 6793 4729
Size: 2064 5830
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2406 1108
Size: 1298 6596
PfoI T'CCnGG_A
Cuts at: 4521 4521
Size: 7894
PmeI GTTT'AAAC
Cuts at: 5198 5198
Size: 7894
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5963 5995 7476 7508 5963
Size: 32 1481 32 6349
Fragments arranged by size:
6349 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2500 2657 4592 2500
Size: 157 1935 5802
Fragments arranged by size:
5802 1935 157
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7894
PsiI TTA'TAA
Cuts at: 2631 3270 5464 2631
Size: 639 2194 5061
Fragments arranged by size:
5061 2194 639
PspOMI G'GGCC_C
Cuts at: 4866 4866
Size: 7894
PstI C_TGCA'G
Cuts at: 830 4602 5246 830
Size: 3772 644 3478
Fragments arranged by size:
3772 3478 644
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7894
PvuII CAG'CTG
Cuts at: 5167 5167
Size: 7894
SacI G_AGCT'C
Cuts at: 3107 4674 3107
Size: 1567 6327
SacII CC_GC'GG
Cuts at: 2026 2076 2304 2026
Size: 50 228 7616
Fragments arranged by size:
7616 228 50
SalI G'TCGA_C
Cuts at: 1936 5236 1936
Size: 3300 4594
SapI GCTCTTCn'nnn_
Cuts at: 3680 3680
Size: 7894
SbfI CC_TGCA'GG
Cuts at: 5246 5246
Size: 7894
ScaI AGT'ACT
Cuts at: 6142 7854 6142
Size: 1712 6182
SfcI C'TryA_G
Cuts at: 826 1045 3468 3880 4353 4598 5242 6377 7058
Size: 219 2423 412 473 245 644 1135 681
Cuts at: 7058 7249 826
Size: 191 1471
Fragments arranged by size:
2423 1471 1135 681 644 473 412 245
219 191
SfoI GGC'GCC
Cuts at: 2046 2235 2046
Size: 189 7705
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7894
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7894
SmaI CCC'GGG
Cuts at: 1872 2014 2033 4550 5221 1872
Size: 142 19 2517 671 4545
Fragments arranged by size:
4545 2517 671 142 19
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4250 5998 6899 7161 820
Size: 197 926 2307 1748 901 262 1553
Fragments arranged by size:
2307 1748 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5685 493
Size: 5192 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7894
SphI G_CATG'C
Cuts at: 1132 5252 1132
Size: 4120 3774
SspI AAT'ATT
Cuts at: 5 52 3870 4492 5818 5
Size: 47 3818 622 1326 2081
Fragments arranged by size:
3818 2081 1326 622 47
StuI AGG'CCT
Cuts at: 1517 3852 1517
Size: 2335 5559
StyI C'CwwG_G
Cuts at: 513 1065 2160 2294 2803 4186 5333 513
Size: 552 1095 134 509 1383 1147 3074
Fragments arranged by size:
3074 1383 1147 1095 552 509 134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3404 3507 5168 5921 5938 6091 6276
Size: 1255 1824 103 1661 753 17 153 185
Cuts at: 6276 6721 325
Size: 445 1498
Fragments arranged by size:
1824 1661 1498 1255 753 445 185 153
103 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2148 4146 4726
Size: 274 80 33 51 1443 171 1998 580
Cuts at: 4726 4775 6140 7852 96
Size: 49 1365 1712 138
Fragments arranged by size:
1998 1712 1443 1365 580 274 171 138
80 51 49 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2406 2430 2438 2462 3324 3356 4449 4481 7854
Size: 24 8 24 862 32 1093 32 3373
Cuts at: 7854 7886 2406
Size: 32 2414
Fragments arranged by size:
3373 2414 1093 862 32 32 32 24
24 8
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7894
XbaI T'CTAG_A
Cuts at: 5230 5230
Size: 7894
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3134 4525 3134
Size: 1391 6503
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7894
XmaI C'CCGG_G
Cuts at: 1870 2012 2031 4548 5219 1870
Size: 142 19 2517 671 4545
Fragments arranged by size:
4545 2517 671 142 19
XmnI GAAnn'nnTTC
Cuts at: 4560 6023 4560
Size: 1463 6431
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5702 276
Size: 53 83 186 936 4168 2468
Fragments arranged by size:
4168 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI
BstZ17I DraIII FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI RsrII SanDI SexAI
SfiI SrfI SwaI