Restriction map for
pFN21AB7337
(Circular) (Six-base) MAPSORT of: pFN21AB7337.seq Check: 7375 from: 1 to: 7834
pFN21AB7337 7834 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2033 2033
Size: 7834
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5644 278
Size: 53 83 186 936 4108 2468
Fragments arranged by size:
4108 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5108 5177 1937
Size: 3171 69 4594
Fragments arranged by size:
4594 3171 69
Acc65I G'GTAC_C
Cuts at: 2039 3009 5155 2039
Size: 970 2146 4718
Fragments arranged by size:
4718 2146 970
AclI AA'CG_TT
Cuts at: 1563 5961 6334 1563
Size: 4398 373 3063
Fragments arranged by size:
4398 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3329 4322 4354 4880 5897 7281 7549 1632
Size: 1697 993 32 526 1017 1384 268 1917
Fragments arranged by size:
1917 1697 1384 1017 993 526 268 32
AflII C'TTAA_G
Cuts at: 820 1017 3116 820
Size: 197 2099 5538
Fragments arranged by size:
5538 2099 197
AflIII A'CryG_T
Cuts at: 1203 3718 4519 6733 7710 1203
Size: 2515 801 2214 977 1327
Fragments arranged by size:
2515 2214 1327 977 801
AgeI A'CCGG_T
Cuts at: 6665 6665
Size: 7834
AhdI GACnn_n'nnGTC
Cuts at: 2137 6563 2137
Size: 4426 3408
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7834
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4669 1184
Size: 3485 4349
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3283 3315 3283
Size: 32 7802
AlwNI CAG_nnn'CTG
Cuts at: 2125 4282 4789 5577 7149 2125
Size: 2157 507 788 1572 2810
Fragments arranged by size:
2810 2157 1572 788 507
ApaI G_GGCC'C
Cuts at: 3451 3451
Size: 7834
ApaLI G'TGCA_C
Cuts at: 5891 7047 5891
Size: 1156 6678
ApoI r'AATT_y
Cuts at: 1493 2096 3668 4107 4792 5143 5369 5521 7630
Size: 603 1572 439 685 351 226 152 2109
Cuts at: 7630 1493
Size: 1697
Fragments arranged by size:
2109 1697 1572 685 603 439 351 226
152
AseI AT'TA_AT
Cuts at: 160 1032 6388 160
Size: 872 5356 1606
Fragments arranged by size:
5356 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7834
AvaI C'yCGr_G
Cuts at: 1870 1943 5129 5159 1870
Size: 73 3186 30 4545
Fragments arranged by size:
4545 3186 73 30
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2023 2056 2023
Size: 33 7801
BamHI G'GATC_C
Cuts at: 2662 5164 5531 2662
Size: 2502 367 4965
Fragments arranged by size:
4965 2502 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2039 3009 5155 6610 618
Size: 325 217 627 252 970 2146 1455 1842
Fragments arranged by size:
2146 1842 1455 970 627 325 252 217
BanII G_rGCy'C
Cuts at: 1391 2683 3021 3451 4626 5153 1391
Size: 1292 338 430 1175 527 4072
Fragments arranged by size:
4072 1292 1175 527 430 338
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2732 3513 3954 928
Size: 971 833 781 441 4808
Fragments arranged by size:
4808 971 833 781 441
BbvCI CC'TCA_GC
Cuts at: 4847 4847
Size: 7834
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2864 2898 6025 6059 1903
Size: 34 927 34 3127 34 3678
Fragments arranged by size:
3678 3127 927 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2177 5059 5726 6936 2177
Size: 2882 667 1210 3075
Fragments arranged by size:
3075 2882 1210 667
BclI T'GATC_A
Cuts at: 1548 3084 1548
Size: 1536 6298
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6445 136
Size: 107 122 71 631 605 4773 1525
Fragments arranged by size:
4773 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2365 3112 7829 2365
Size: 747 4717 2370
Fragments arranged by size:
4717 2370 747
BlpI GC'TnA_GC
Cuts at: 5251 5251
Size: 7834
Bme1580I G_kGCm'C
Cuts at: 1790 2157 3277 3451 5895 7051 1790
Size: 367 1120 174 2444 1156 2573
Fragments arranged by size:
2573 2444 1156 1120 367 174
BmgBI CAC'GTC
Cuts at: 1318 3721 4287 1318
Size: 2403 566 4865
Fragments arranged by size:
4865 2403 566
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2767 3017 5565
Size: 416 53 83 399 24 1350 250 2548
Cuts at: 5565 6523 442
Size: 958 1753
Fragments arranged by size:
2548 1753 1350 958 416 399 250 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7834
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2986 2986
Size: 7834
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2111 2591 3323 3735 4133 4475 4640 6494
Size: 872 480 732 412 398 342 165 1854
Cuts at: 6494 1239
Size: 2579
Fragments arranged by size:
2579 1854 872 732 480 412 398 342
165
Bpu10I CC'TnA_GC
Cuts at: 4064 4847 4064
Size: 783 7051
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2203 3193 4424 5959 6824 7122 2203
Size: 990 1231 1535 865 298 2915
Fragments arranged by size:
2915 1535 1231 990 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4244 6497 882
Size: 617 2745 2253 2219
Fragments arranged by size:
2745 2253 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2608 4522 5625 493
Size: 711 1404 1914 1103 2702
Fragments arranged by size:
2702 1914 1404 1103 711
BsaBI GATnn'nnATC
Cuts at: 2082 3089 7828 2082
Size: 1007 4739 2088
Fragments arranged by size:
4739 2088 1007
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5641 6023 6658 275
Size: 53 83 186 936 4108 382 635 1451
Fragments arranged by size:
4108 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6267 6665 6939 7086 7497 6267
Size: 398 274 147 411 6604
Fragments arranged by size:
6604 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3380 3410 4613 4643 1178
Size: 30 165 30 1977 30 1203 30 4369
Fragments arranged by size:
4369 1977 1203 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7834
BseYI C'CCAG_C
Cuts at: 2118 4384 7037 7533 2118
Size: 2266 2653 496 2419
Fragments arranged by size:
2653 2419 2266 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2351 2738 1163
Size: 573 615 387 6259
Fragments arranged by size:
6259 615 573 387
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5284 6045 7073 1540
Size: 275 186 3283 761 1028 2301
Fragments arranged by size:
3283 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3021 4626 5895 5980 7051 3021
Size: 1605 1269 85 1071 3804
Fragments arranged by size:
3804 1605 1269 1071 85
BsmI GAATG_Cn'
Cuts at: 5345 5438 5345
Size: 93 7741
BsmBI CGTCTCn'nnnn_
Cuts at: 4722 4722
Size: 7834
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2157 2683 3021 3277 3451 4626 5153
Size: 399 367 526 338 256 174 1175 527
Cuts at: 5153 5895 5980 7051 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1175 1071 742 527 526 399 367
338 256 174 85
BspEI T'CCGG_A
Cuts at: 7497 7497
Size: 7834
BspHI T'CATG_A
Cuts at: 3189 5723 7453 3189
Size: 2534 1730 3570
Fragments arranged by size:
3570 2534 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2033 4788 5189 844
Size: 1189 2755 401 3489
Fragments arranged by size:
3489 2755 1189 401
BsrBI CCG'CTC
Cuts at: 1394 1823 5721 1394
Size: 429 3898 3507
Fragments arranged by size:
3898 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2745 4946 5062 6329 6503 65
Size: 1183 1497 2201 116 1267 174 1396
Fragments arranged by size:
2201 1497 1396 1267 1183 174 116
BsrFI r'CCGG_y
Cuts at: 1700 1810 6478 6665 1700
Size: 110 4668 187 2869
Fragments arranged by size:
4668 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2467 2999 3145 96
Size: 2371 532 146 4785
Fragments arranged by size:
4785 2371 532 146
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7834
BssSI C'ACGA_G
Cuts at: 3932 5894 6906 3932
Size: 1962 1012 4860
Fragments arranged by size:
4860 1962 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2749 794
Size: 1955 5879
BstBI TT'CG_AA
Cuts at: 6638 6638
Size: 7834
BstEII G'GTnAC_C
Cuts at: 1184 4775 1184
Size: 3591 4243
BstXI CCAn_nnnn'nTGG
Cuts at: 3441 3841 4562 3441
Size: 400 721 6713
Fragments arranged by size:
6713 721 400
BstYI r'GATC_y
Cuts at: 1972 2350 2365 2419 2488 2662 3112 5164 5531
Size: 378 15 54 69 174 450 2052 367
Cuts at: 5531 5916 5933 7385 7471 7483 7829 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
2052 1977 1452 450 385 378 367 346
174 86 69 54 17 15 12
BtgI C'CryG_G
Cuts at: 513 1065 3279 513
Size: 552 2214 5068
Fragments arranged by size:
5068 2214 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7095
Fragments arranged by size:
7095 683 56
BtsI GCAGTG_nn'
Cuts at: 3277 3922 5350 6144 6164 3277
Size: 645 1428 794 20 4947
Fragments arranged by size:
4947 1428 794 645 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7834
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3568 3603 529
Size: 35 3004 35 4760
Fragments arranged by size:
4760 3004 35 35
DraI TTT'AAA
Cuts at: 3780 5138 3780
Size: 1358 6476
DraIII CAC_nnn'GTG
Cuts at: 2297 4943 2297
Size: 2646 5188
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4736 6841 809
Size: 507 3420 2105 1802
Fragments arranged by size:
3420 2105 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3431 3462 5281
Size: 54 1425 50 275 18 1601 31 1819
Cuts at: 5281 6170 7799 8
Size: 889 1629 43
Fragments arranged by size:
1819 1629 1601 1425 889 275 54 50
43 31 18
EagI C'GGCC_G
Cuts at: 1537 1812 5281 1537
Size: 275 3469 4090
Fragments arranged by size:
4090 3469 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2889 3861 5009 5764 1353
Size: 1536 972 1148 755 3423
Fragments arranged by size:
3423 1536 1148 972 755
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6421 6795 6941 387
Size: 1064 138 4832 374 146 1280
Fragments arranged by size:
4832 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3019 4624 3019
Size: 1605 6229
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2111 2591 3323 3329 3735 4133 4322
Size: 393 479 480 732 6 406 398 189
Cuts at: 4322 4354 4475 4640 4880 5897 6494 7281 7549
Size: 32 121 165 240 1017 597 787 268
Cuts at: 7549 1239
Size: 1524
Fragments arranged by size:
1524 1017 787 732 597 480 479 406
398 393 268 240 189 165 121 32
6
EcoNI CCTnn'n_nnAGG
Cuts at: 4751 4751
Size: 7834
EcoO109I rG'GnC_Cy
Cuts at: 3447 3448 3523 4321 4489 3447
Size: 1 75 798 168 6792
Fragments arranged by size:
6792 798 168 75 1
EcoRI G'AATT_C
Cuts at: 2096 3668 5143 5521 2096
Size: 1572 1475 378 4409
Fragments arranged by size:
4409 1572 1475 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2125 2749 4007 4624 2125
Size: 624 1258 617 5335
Fragments arranged by size:
5335 1258 624 617
FspI TGC'GCA
Cuts at: 6340 6340
Size: 7834
HaeII r_GCGC'y
Cuts at: 1678 2656 6981 1678
Size: 978 4325 2531
Fragments arranged by size:
4325 2531 978
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2123 2155 2266 2298 2888
Size: 32 135 32 551 32 111 32 590
Cuts at: 2888 2920 2975 3007 3326 3358 3955 3987 4257
Size: 32 55 32 319 32 597 32 270
Cuts at: 4257 4289 4608 4640 6475 6507 6549 6581 1373
Size: 32 319 32 1835 32 42 32 2626
Fragments arranged by size:
2626 1835 597 590 551 319 319 270
135 111 55 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3988 5109 5178 5424 669
Size: 988 281 2050 1121 69 246 3079
Fragments arranged by size:
3079 2050 1121 988 281 246 69
HindIII A'AGCT_T
Cuts at: 748 2102 4020 4413 748
Size: 1354 1918 393 4169
Fragments arranged by size:
4169 1918 1354 393
HpaI GTT'AAC
Cuts at: 5424 5424
Size: 7834
KpnI G_GTAC'C
Cuts at: 2043 3013 5159 2043
Size: 970 2146 4718
Fragments arranged by size:
4718 2146 970
MfeI C'AATT_G
Cuts at: 5433 5433
Size: 7834
MluI A'CGCG_T
Cuts at: 7710 7710
Size: 7834
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2378 3269 3411 4540 6948 7132 1305
Size: 1073 891 142 1129 2408 184 2007
Fragments arranged by size:
2408 2007 1129 1073 891 184 142
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3433 3464 10
Size: 54 1425 343 1601 31 4380
Fragments arranged by size:
4380 1601 1425 343 54 31
MslI CAynn'nnrTG
Cuts at: 518 1166 3201 5792 6151 6310 518
Size: 648 2035 2591 359 159 2042
Fragments arranged by size:
2591 2042 2035 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2089 2125 2811 3429 4482 4639 5250 5927
Size: 483 36 686 618 1053 157 611 677
Cuts at: 5927 7075 7320 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1053 686 677 618 611 483
245 157 36
NcoI C'CATG_G
Cuts at: 513 1065 3279 513
Size: 552 2214 5068
Fragments arranged by size:
5068 2214 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7834
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7834
NotI GC'GGCC_GC
Cuts at: 5281 5281
Size: 7834
NspI r_CATG'y
Cuts at: 1132 1229 5115 5192 6737 1132
Size: 97 3886 77 1545 2229
Fragments arranged by size:
3886 2229 1545 97 77
PasI CC'CwG_GG
Cuts at: 1348 2149 2172 2407 2476 3834 1348
Size: 801 23 235 69 1358 5348
Fragments arranged by size:
5348 1358 801 235 69 23
PciI A'CATG_T
Cuts at: 6733 6733
Size: 7834
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7834
PfoI T'CCnGG_A
Cuts at: 3621 4133 3621
Size: 512 7322
PmeI GTTT'AAAC
Cuts at: 5138 5138
Size: 7834
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4358 4390 5903 5935 7416 7448 4358
Size: 32 1513 32 1481 32 4744
Fragments arranged by size:
4744 1513 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4321 4489 4321
Size: 168 7666
PshAI GACnn'nnGTC
Cuts at: 1613 2696 1613
Size: 1083 6751
PsiI TTA'TAA
Cuts at: 5404 5404
Size: 7834
PspOMI G'GGCC_C
Cuts at: 3447 3447
Size: 7834
PstI C_TGCA'G
Cuts at: 830 2652 3234 3272 3458 4320 4683 4785 5124
Size: 1822 582 38 186 862 363 102 339
Cuts at: 5124 5186 830
Size: 62 3478
Fragments arranged by size:
3478 1822 862 582 363 339 186 102
62 38
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7834
PvuII CAG'CTG
Cuts at: 2089 2125 2811 3429 4482 4639 2089
Size: 36 686 618 1053 157 5284
Fragments arranged by size:
5284 1053 686 618 157 36
SacI G_AGCT'C
Cuts at: 3021 4626 3021
Size: 1605 6229
SalI G'TCGA_C
Cuts at: 1936 5107 5176 1936
Size: 3171 69 4594
Fragments arranged by size:
4594 3171 69
SbfI CC_TGCA'GG
Cuts at: 5186 5186
Size: 7834
ScaI AGT'ACT
Cuts at: 6082 7794 6082
Size: 1712 6122
SexAI A'CCwGG_T
Cuts at: 3098 3098
Size: 7834
SfcI C'TryA_G
Cuts at: 826 1045 2648 3230 3268 3454 4316 4679 4781
Size: 219 1603 582 38 186 862 363 102
Cuts at: 4781 5120 5182 6317 6998 7189 826
Size: 339 62 1135 681 191 1471
Fragments arranged by size:
1603 1471 1135 862 681 582 363 339
219 191 186 102 62 38
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7834
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7834
SmaI CCC'GGG
Cuts at: 1872 5161 1872
Size: 3289 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2182 3116 3208 4439 5938 6839
Size: 197 926 239 934 92 1231 1499 901
Cuts at: 6839 7101 820
Size: 262 1553
Fragments arranged by size:
1553 1499 1231 934 926 901 262 239
197 92
SnaBI TAC'GTA
Cuts at: 493 5625 493
Size: 5132 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7834
SphI G_CATG'C
Cuts at: 1132 5192 1132
Size: 4060 3774
SspI AAT'ATT
Cuts at: 5 52 3565 3997 5758 5
Size: 47 3513 432 1761 2081
Fragments arranged by size:
3513 2081 1761 432 47
StuI AGG'CCT
Cuts at: 1517 3122 4757 1517
Size: 1605 1635 4594
Fragments arranged by size:
4594 1635 1605
StyI C'CwwG_G
Cuts at: 513 1065 2047 2733 3279 4758 5273 513
Size: 552 982 686 546 1479 515 3074
Fragments arranged by size:
3074 1479 982 686 552 546 515
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3623 5861 5878 6031 6216 6661 325
Size: 1255 2043 2238 17 153 185 445 1498
Fragments arranged by size:
2238 2043 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2467 2999 3145
Size: 274 80 33 51 1443 490 532 146
Cuts at: 3145 6080 7792 96
Size: 2935 1712 138
Fragments arranged by size:
2935 1712 1443 532 490 274 146 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7794 7826 7794
Size: 32 7802
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7834
XbaI T'CTAG_A
Cuts at: 2368 5170 2368
Size: 2802 5032
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3003 3003
Size: 7834
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7834
XmaI C'CCGG_G
Cuts at: 1870 5159 1870
Size: 3289 4545
XmnI GAAnn'nnTTC
Cuts at: 3063 5963 3063
Size: 2900 4934
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5642 276
Size: 53 83 186 936 4108 2468
Fragments arranged by size:
4108 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbeI BfrBI BsiWI BstZ17I Bsu36I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI
SapI SfiI SfoI SrfI SwaI