Restriction map for
pFN21AB7332
(Circular) (Six-base) MAPSORT of: pFN21AB7332.seq Check: 3343 from: 1 to: 7801
pFN21AB7332 7801 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5611 278
Size: 53 83 186 936 4075 2468
Fragments arranged by size:
4075 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2685 5144 1937
Size: 748 2459 4594
Fragments arranged by size:
4594 2459 748
Acc65I G'GTAC_C
Cuts at: 4486 5122 4486
Size: 636 7165
AclI AA'CG_TT
Cuts at: 1563 4924 5928 6301 1563
Size: 3361 1004 373 3063
Fragments arranged by size:
3361 3063 1004 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2229 3251 3785 3797 4782 5864 7248 7516
Size: 597 1022 534 12 985 1082 1384 268
Cuts at: 7516 1632
Size: 1917
Fragments arranged by size:
1917 1384 1082 1022 985 597 534 268
12
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7604
AflIII A'CryG_T
Cuts at: 1203 4030 6700 7677 1203
Size: 2827 2670 977 1327
Fragments arranged by size:
2827 2670 1327 977
AgeI A'CCGG_T
Cuts at: 6632 6632
Size: 7801
AhdI GACnn_n'nnGTC
Cuts at: 6530 6530
Size: 7801
AleI CACnn'nnGTG
Cuts at: 1166 4937 1166
Size: 3771 4030
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4623 1184
Size: 3439 4362
AlwNI CAG_nnn'CTG
Cuts at: 5544 7116 5544
Size: 1572 6229
ApaI G_GGCC'C
Cuts at: 2144 2650 2144
Size: 506 7295
ApaLI G'TGCA_C
Cuts at: 5858 7014 5858
Size: 1156 6645
ApoI r'AATT_y
Cuts at: 1493 2573 2584 2741 3452 3788 4492 4699 5110
Size: 1080 11 157 711 336 704 207 411
Cuts at: 5110 5336 5488 7597 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1080 711 704 411 336 226
207 157 152 11
AseI AT'TA_AT
Cuts at: 160 1032 6355 160
Size: 872 5323 1606
Fragments arranged by size:
5323 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7801
AvaI C'yCGr_G
Cuts at: 1870 1943 2258 3043 5126 1870
Size: 73 315 785 2083 4545
Fragments arranged by size:
4545 2083 785 315 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4216 4249 4216
Size: 33 7768
BamHI G'GATC_C
Cuts at: 2420 5131 5498 2420
Size: 2711 367 4723
Fragments arranged by size:
4723 2711 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4486 5122 6577 618
Size: 325 217 627 2699 636 1455 1842
Fragments arranged by size:
2699 1842 1455 636 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2144 2650 2821 3175 4205 5120 1391
Size: 753 506 171 354 1030 915 4072
Fragments arranged by size:
4072 1030 915 753 506 354 171
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3974 928
Size: 971 2075 4755
Fragments arranged by size:
4755 2075 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5992 6026 1903
Size: 34 4055 34 3678
Fragments arranged by size:
4055 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5693 6903 5693
Size: 1210 6591
BclI T'GATC_A
Cuts at: 1548 2250 1548
Size: 702 7099
BfrBI ATG'CAT
Cuts at: 4013 4013
Size: 7801
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6412 136
Size: 107 122 71 631 605 4740 1525
Fragments arranged by size:
4740 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2968 3493 7796 2968
Size: 525 4303 2973
Fragments arranged by size:
4303 2973 525
BlpI GC'TnA_GC
Cuts at: 5218 5218
Size: 7801
Bme1580I G_kGCm'C
Cuts at: 1790 2144 2511 2650 3874 5862 7018 1790
Size: 354 367 139 1224 1988 1156 2573
Fragments arranged by size:
2573 1988 1224 1156 367 354 139
BmgBI CAC'GTC
Cuts at: 1318 2304 3631 1318
Size: 986 1327 5488
Fragments arranged by size:
5488 1327 986
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5532 6490 442
Size: 416 53 83 399 24 4115 958 1753
Fragments arranged by size:
4115 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7801
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2300 4023 4572 6461 1239
Size: 1061 1723 549 1889 2579
Fragments arranged by size:
2579 1889 1723 1061 549
Bpu10I CC'TnA_GC
Cuts at: 3386 3386
Size: 7801
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4153 4790 5926 6791 7089 4153
Size: 637 1136 865 298 4865
Fragments arranged by size:
4865 1136 865 637 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6464 882
Size: 617 4965 2219
Fragments arranged by size:
4965 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5592 493
Size: 711 4388 2702
Fragments arranged by size:
4388 2702 711
BsaBI GATnn'nnATC
Cuts at: 7795 7795
Size: 7801
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5608 5990 6625 275
Size: 53 83 186 936 4075 382 635 1451
Fragments arranged by size:
4075 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4635 6234 6632 6906 7053 7464 4635
Size: 1599 398 274 147 411 4972
Fragments arranged by size:
4972 1599 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2916 2946 1178
Size: 30 165 30 1513 30 6033
Fragments arranged by size:
6033 1513 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2811 2829 1186
Size: 1625 18 6158
Fragments arranged by size:
6158 1625 18
BseYI C'CCAG_C
Cuts at: 2320 7004 7500 2320
Size: 4684 496 2621
Fragments arranged by size:
4684 2621 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2162 3243 1163
Size: 573 426 1081 5721
Fragments arranged by size:
5721 1081 573 426
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2094 2098 2172 5251 6012 7040
Size: 275 186 93 4 74 3079 761 1028
Cuts at: 7040 1540
Size: 2301
Fragments arranged by size:
3079 2301 1028 761 275 186 93 74
4
BsiHKAI G_wGCw'C
Cuts at: 2821 2986 2999 3175 4205 5862 5947 7018 2821
Size: 165 13 176 1030 1657 85 1071 3604
Fragments arranged by size:
3604 1657 1071 1030 176 165 85 13
BsmI GAATG_Cn'
Cuts at: 3064 4562 5312 5405 3064
Size: 1498 750 93 5460
Fragments arranged by size:
5460 1498 750 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2051 2051
Size: 7801
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2144 2511 2650 2821 2986 2999 3175
Size: 399 354 367 139 171 165 13 176
Cuts at: 3175 3874 4205 5120 5862 5947 7018 1391
Size: 699 331 915 742 85 1071 2174
Fragments arranged by size:
2174 1071 915 742 699 399 367 354
331 176 171 165 139 85 13
BspEI T'CCGG_A
Cuts at: 7464 7464
Size: 7801
BspHI T'CATG_A
Cuts at: 5690 7420 5690
Size: 1730 6071
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5156 844
Size: 4312 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4217 5688 1394
Size: 429 2394 1471 3507
Fragments arranged by size:
3507 2394 1471 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3069 4617 5098 6296 6470 65
Size: 1183 1821 1548 481 1198 174 1396
Fragments arranged by size:
1821 1548 1396 1198 1183 481 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2093 4767 6445 6632 1700
Size: 110 283 2674 1678 187 2869
Fragments arranged by size:
2869 2674 1678 283 187 110
BsrGI T'GTAC_A
Cuts at: 96 3027 96
Size: 2931 4870
BssHII G'CGCG_C
Cuts at: 1925 2124 1925
Size: 199 7602
BssSI C'ACGA_G
Cuts at: 2975 5861 6873 2975
Size: 2886 1012 3903
Fragments arranged by size:
3903 2886 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3356 794
Size: 2562 5239
BstBI TT'CG_AA
Cuts at: 6605 6605
Size: 7801
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7801
BstYI r'GATC_y
Cuts at: 1972 2420 2968 3231 3493 5131 5498 5883 5900
Size: 448 548 263 262 1638 367 385 17
Cuts at: 5900 7352 7438 7450 7796 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1638 1452 548 448 385 367 346
263 262 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2185 4044 513
Size: 552 939 181 1859 4270
Fragments arranged by size:
4270 1859 939 552 181
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7062
Fragments arranged by size:
7062 683 56
BtsI GCAGTG_nn'
Cuts at: 2220 5317 6111 6131 2220
Size: 3097 794 20 3890
Fragments arranged by size:
3890 3097 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7801
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7766
DraI TTT'AAA
Cuts at: 4314 4698 5020 5105 4314
Size: 384 322 85 7010
Fragments arranged by size:
7010 384 322 85
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6808 809
Size: 507 5492 1802
Fragments arranged by size:
5492 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2095 2296 3217
Size: 54 1425 50 275 18 265 201 921
Cuts at: 3217 4769 5248 6137 7766 8
Size: 1552 479 889 1629 43
Fragments arranged by size:
1629 1552 1425 921 889 479 275 265
201 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2095 5248 1537
Size: 275 283 3153 4090
Fragments arranged by size:
4090 3153 283 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2358 3010 3616 4074 4215 4257 5053 5731
Size: 1005 652 606 458 141 42 796 678
Cuts at: 5731 1353
Size: 3423
Fragments arranged by size:
3423 1005 796 678 652 606 458 141
42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6388 6762 6908 387
Size: 1064 138 4799 374 146 1280
Fragments arranged by size:
4799 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2819 3173 4203 2819
Size: 354 1030 6417
Fragments arranged by size:
6417 1030 354
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2229 2300 3251 3785 3797 4023 4572
Size: 393 597 71 951 534 12 226 549
Cuts at: 4572 4782 5864 6461 7248 7516 1239
Size: 210 1082 597 787 268 1524
Fragments arranged by size:
1524 1082 951 787 597 597 549 534
393 268 226 210 71 12
EcoO109I rG'GnC_Cy
Cuts at: 2086 2140 2141 2646 2647 4225 2086
Size: 54 1 505 1 1578 5662
Fragments arranged by size:
5662 1578 505 54 1 1
EcoRI G'AATT_C
Cuts at: 3788 5110 5488 3788
Size: 1322 378 6101
Fragments arranged by size:
6101 1322 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5029 5029
Size: 7801
FseI GG_CCGG'CC
Cuts at: 4771 4771
Size: 7801
FspI TGC'GCA
Cuts at: 4884 6307 4884
Size: 1423 6378
HaeII r_GCGC'y
Cuts at: 1678 6948 1678
Size: 5270 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2265 2297 2359 2391 2659
Size: 32 135 32 693 32 62 32 268
Cuts at: 2659 2691 2751 2783 2916 2948 2980 3218 3250
Size: 32 60 32 133 32 32 238 32
Cuts at: 3250 3255 3287 4151 4183 4187 4219 6442 6474
Size: 5 32 864 32 4 32 2223 32
Cuts at: 6474 6516 6548 1373
Size: 42 32 2626
Fragments arranged by size:
2626 2223 864 693 268 238 135 133
62 60 42 32 32 32 32 32
32 32 32 32 32 32 32 32
32 5 4
HincII GTy'rAC
Cuts at: 669 1657 1938 3240 3412 5145 5391 669
Size: 988 281 1302 172 1733 246 3079
Fragments arranged by size:
3079 1733 1302 988 281 246 172
HindIII A'AGCT_T
Cuts at: 748 2906 748
Size: 2158 5643
HpaI GTT'AAC
Cuts at: 5391 5391
Size: 7801
KpnI G_GTAC'C
Cuts at: 4490 5126 4490
Size: 636 7165
MfeI C'AATT_G
Cuts at: 5400 5400
Size: 7801
MluI A'CGCG_T
Cuts at: 7677 7677
Size: 7801
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2749 2791 3061 4780 5048 6915 7099 1305
Size: 1444 42 270 1719 268 1867 184 2007
Fragments arranged by size:
2007 1867 1719 1444 270 268 184 42
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5979
Fragments arranged by size:
5979 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3650 4937 5759 6118 6277 518
Size: 648 2484 1287 822 359 159 2042
Fragments arranged by size:
2484 2042 1287 822 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2320 5217 5894 7042 7287 1606
Size: 714 2897 677 1148 245 2120
Fragments arranged by size:
2897 2120 1148 714 677 245
NaeI GCC'GGC
Cuts at: 4769 4769
Size: 7801
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6310
Fragments arranged by size:
6310 939 552
NdeI CA'TA_TG
Cuts at: 387 4015 4993 387
Size: 3628 978 3195
Fragments arranged by size:
3628 3195 978
NgoMIV G'CCGG_C
Cuts at: 4767 4767
Size: 7801
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7801
NotI GC'GGCC_GC
Cuts at: 5248 5248
Size: 7801
NsiI A_TGCA'T
Cuts at: 4015 4015
Size: 7801
NspI r_CATG'y
Cuts at: 1132 1229 4034 4247 4889 5159 6704 1132
Size: 97 2805 213 642 270 1545 2229
Fragments arranged by size:
2805 2229 1545 642 270 213 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7801
PciI A'CATG_T
Cuts at: 4030 6700 4030
Size: 2670 5131
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 6899
PfoI T'CCnGG_A
Cuts at: 2052 4191 2052
Size: 2139 5662
PmeI GTTT'AAAC
Cuts at: 5105 5105
Size: 7801
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5870 5902 7383 7415 5870
Size: 32 1481 32 6256
Fragments arranged by size:
6256 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2086 4225 2086
Size: 2139 5662
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7801
PsiI TTA'TAA
Cuts at: 3555 5371 3555
Size: 1816 5985
PspOMI G'GGCC_C
Cuts at: 2140 2646 2140
Size: 506 7295
PstI C_TGCA'G
Cuts at: 830 4139 5153 830
Size: 3309 1014 3478
Fragments arranged by size:
3478 3309 1014
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7801
PvuII CAG'CTG
Cuts at: 2320 2320
Size: 7801
SacI G_AGCT'C
Cuts at: 2821 3175 4205 2821
Size: 354 1030 6417
Fragments arranged by size:
6417 1030 354
SalI G'TCGA_C
Cuts at: 1936 5143 1936
Size: 3207 4594
SanDI GG'GwC_CC
Cuts at: 2086 2086
Size: 7801
SbfI CC_TGCA'GG
Cuts at: 5153 5153
Size: 7801
ScaI AGT'ACT
Cuts at: 6049 7761 6049
Size: 1712 6089
SfcI C'TryA_G
Cuts at: 826 1045 3117 3399 3673 4135 4299 5149 6284
Size: 219 2072 282 274 462 164 850 1135
Cuts at: 6284 6965 7156 826
Size: 681 191 1471
Fragments arranged by size:
2072 1471 1135 850 681 462 282 274
219 191 164
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7801
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7801
SmaI CCC'GGG
Cuts at: 1872 2260 5128 1872
Size: 388 2868 4545
Fragments arranged by size:
4545 2868 388
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4168 4805 5905 6806 7068 820
Size: 197 926 2225 637 1100 901 262 1553
Fragments arranged by size:
2225 1553 1100 926 901 637 262 197
SnaBI TAC'GTA
Cuts at: 493 5592 493
Size: 5099 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7801
SphI G_CATG'C
Cuts at: 1132 4247 5159 1132
Size: 3115 912 3774
Fragments arranged by size:
3774 3115 912
SspI AAT'ATT
Cuts at: 5 52 5725 5
Size: 47 5673 2081
Fragments arranged by size:
5673 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7801
StyI C'CwwG_G
Cuts at: 513 1065 2004 3751 5240 513
Size: 552 939 1747 1489 3074
Fragments arranged by size:
3074 1747 1489 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2158 2201 3001 3354 5828 5845 5998
Size: 1255 578 43 800 353 2474 17 153
Cuts at: 5998 6183 6628 325
Size: 185 445 1498
Fragments arranged by size:
2474 1498 1255 800 578 445 353 185
153 43 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3027 3779 4668
Size: 274 80 33 51 1443 1050 752 889
Cuts at: 4668 4975 6047 7759 96
Size: 307 1072 1712 138
Fragments arranged by size:
1712 1443 1072 1050 889 752 307 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2412 2444 7761 7793 2412
Size: 32 5317 32 2420
Fragments arranged by size:
5317 2420 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7801
XbaI T'CTAG_A
Cuts at: 5137 5137
Size: 7801
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4471 4547 4783 4471
Size: 76 236 7489
Fragments arranged by size:
7489 236 76
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7801
XmaI C'CCGG_G
Cuts at: 1870 2258 5126 1870
Size: 388 2868 4545
Fragments arranged by size:
4545 2868 388
XmnI GAAnn'nnTTC
Cuts at: 3264 5930 3264
Size: 2666 5135
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5609 276
Size: 53 83 186 936 4075 2468
Fragments arranged by size:
4075 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FspAI
KasI NarI NruI PacI PmlI PspXI PsrI RsrII
SacII SapI SexAI SfiI SfoI SrfI SwaI