Restriction map for
pFN21AB7327
(Circular) (Six-base) MAPSORT of: pFN21AB7327.seq Check: 7312 from: 1 to: 7219
pFN21AB7327 7219 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5029 278
Size: 53 83 186 936 3493 2468
Fragments arranged by size:
3493 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2183 2203 2846 3523 4562 1937
Size: 246 20 643 677 1039 4594
Fragments arranged by size:
4594 1039 677 643 246 20
Acc65I G'GTAC_C
Cuts at: 2196 2679 4540 2196
Size: 483 1861 4875
Fragments arranged by size:
4875 1861 483
AclI AA'CG_TT
Cuts at: 1563 5346 5719 1563
Size: 3783 373 3063
Fragments arranged by size:
3783 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2507 3297 3380 3687 3698 3913 4094 4425
Size: 875 790 83 307 11 215 181 331
Cuts at: 4425 4512 5282 6666 6934 1632
Size: 87 770 1384 268 1917
Fragments arranged by size:
1917 1384 875 790 770 331 307 268
215 181 87 83 11
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7022
AflIII A'CryG_T
Cuts at: 1203 3474 6118 7095 1203
Size: 2271 2644 977 1327
Fragments arranged by size:
2644 2271 1327 977
AgeI A'CCGG_T
Cuts at: 6050 6050
Size: 7219
AhdI GACnn_n'nnGTC
Cuts at: 5948 5948
Size: 7219
AleI CACnn'nnGTG
Cuts at: 1166 2075 1166
Size: 909 6310
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7219
AlwNI CAG_nnn'CTG
Cuts at: 4962 6534 4962
Size: 1572 5647
ApaLI G'TGCA_C
Cuts at: 5276 6432 5276
Size: 1156 6063
ApoI r'AATT_y
Cuts at: 1493 2190 2243 2344 2442 2591 2870 3764 3793
Size: 697 53 101 98 149 279 894 29
Cuts at: 3793 4294 4486 4528 4754 4906 7015 1493
Size: 501 192 42 226 152 2109 1697
Fragments arranged by size:
2109 1697 894 697 501 279 226 192
152 149 101 98 53 42 29
AseI AT'TA_AT
Cuts at: 160 1032 2730 4047 4316 5773 160
Size: 872 1698 1317 269 1457 1606
Fragments arranged by size:
1698 1606 1457 1317 872 269
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7219
AvaI C'yCGr_G
Cuts at: 1870 1943 4544 1870
Size: 73 2601 4545
Fragments arranged by size:
4545 2601 73
BamHI G'GATC_C
Cuts at: 3482 3994 4549 4916 3482
Size: 512 555 367 5785
Fragments arranged by size:
5785 555 512 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2196 2679 4540 5995 618
Size: 325 217 627 409 483 1861 1455 1842
Fragments arranged by size:
1861 1842 1455 627 483 409 325 217
BanII G_rGCy'C
Cuts at: 1391 4538 1391
Size: 3147 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3361 928
Size: 971 1462 4786
Fragments arranged by size:
4786 1462 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5410 5444 1903
Size: 34 50 34 3389 34 3678
Fragments arranged by size:
3678 3389 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5111 6321 5111
Size: 1210 6009
BclI T'GATC_A
Cuts at: 1548 3597 4239 4426 1548
Size: 2049 642 187 4341
Fragments arranged by size:
4341 2049 642 187
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5830 136
Size: 107 122 71 631 605 4158 1525
Fragments arranged by size:
4158 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2518 3435 4390 7214 2518
Size: 917 955 2824 2523
Fragments arranged by size:
2824 2523 955 917
BlpI GC'TnA_GC
Cuts at: 4636 4636
Size: 7219
Bme1580I G_kGCm'C
Cuts at: 1790 5280 6436 1790
Size: 3490 1156 2573
Fragments arranged by size:
3490 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2997 1318
Size: 1679 5540
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2600 4093 4950
Size: 416 53 83 399 24 1183 1493 857
Cuts at: 4950 5908 442
Size: 958 1753
Fragments arranged by size:
1753 1493 1183 958 857 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7219
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2031 2682 5879 1239
Size: 792 651 3197 2579
Fragments arranged by size:
3197 2579 792 651
Bpu10I CC'TnA_GC
Cuts at: 2705 2705
Size: 7219
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5344 6209 6507 5344
Size: 865 298 6056
Fragments arranged by size:
6056 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3012 5882 882
Size: 617 1513 2870 2219
Fragments arranged by size:
2870 2219 1513 617
BsaAI yAC'GTr
Cuts at: 493 1204 5010 493
Size: 711 3806 2702
Fragments arranged by size:
3806 2702 711
BsaBI GATnn'nnATC
Cuts at: 4389 7213 4389
Size: 2824 4395
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5026 5408 6043 275
Size: 53 83 186 936 3493 382 635 1451
Fragments arranged by size:
3493 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2610 5652 6050 6324 6471 6882 2610
Size: 3042 398 274 147 411 2947
Fragments arranged by size:
3042 2947 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3139 3169 1178
Size: 30 165 30 1736 30 5228
Fragments arranged by size:
5228 1736 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2482 2485 2679 2823 1186
Size: 1296 3 194 144 5582
Fragments arranged by size:
5582 1296 194 144 3
BseYI C'CCAG_C
Cuts at: 6422 6918 6422
Size: 496 6723
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3308 1163
Size: 573 1572 5074
Fragments arranged by size:
5074 1572 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4669 5430 6458 1540
Size: 275 186 2668 761 1028 2301
Fragments arranged by size:
2668 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5280 5365 6436 5280
Size: 85 1071 6063
Fragments arranged by size:
6063 1071 85
BsmI GAATG_Cn'
Cuts at: 3828 4441 4730 4823 3828
Size: 613 289 93 6224
Fragments arranged by size:
6224 613 289 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4538 5280 5365 6436 1391
Size: 399 2748 742 85 1071 2174
Fragments arranged by size:
2748 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 2610 6882 2610
Size: 4272 2947
BspHI T'CATG_A
Cuts at: 5108 6838 5108
Size: 1730 5489
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4574 844
Size: 3730 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2140 5106 1394
Size: 429 317 2966 3507
Fragments arranged by size:
3507 2966 429 317
BsrDI GCAATG_nn'
Cuts at: 65 1248 2058 2778 3037 3188 5714 5888 65
Size: 1183 810 720 259 151 2526 174 1396
Fragments arranged by size:
2526 1396 1183 810 720 259 174 151
BsrFI r'CCGG_y
Cuts at: 1700 1810 5863 6050 1700
Size: 110 4053 187 2869
Fragments arranged by size:
4053 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7219
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7219
BssSI C'ACGA_G
Cuts at: 4026 5279 6291 4026
Size: 1253 1012 4954
Fragments arranged by size:
4954 1253 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3322 794
Size: 2528 4691
BstBI TT'CG_AA
Cuts at: 2540 6023 2540
Size: 3483 3736
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7219
BstYI r'GATC_y
Cuts at: 1972 2518 3435 3482 3994 4105 4303 4390 4549
Size: 546 917 47 512 111 198 87 159
Cuts at: 4549 4916 5301 5318 6770 6856 6868 7214 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 917 546 512 385 367 346
198 159 111 87 86 47 17 12
Bsu36I CC'TnA_GG
Cuts at: 4308 4308
Size: 7219
BtgI C'CryG_G
Cuts at: 513 1065 2022 513
Size: 552 957 5710
Fragments arranged by size:
5710 957 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2689 487
Size: 683 56 1463 5017
Fragments arranged by size:
5017 1463 683 56
BtsI GCAGTG_nn'
Cuts at: 2070 4735 5529 5549 2070
Size: 2665 794 20 3740
Fragments arranged by size:
3740 2665 794 20
ClaI AT'CG_AT
Cuts at: 1554 3774 4188 1554
Size: 2220 414 4585
Fragments arranged by size:
4585 2220 414
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7184
DraI TTT'AAA
Cuts at: 2325 4523 2325
Size: 2198 5021
DraIII CAC_nnn'GTG
Cuts at: 3856 3856
Size: 7219
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6226 809
Size: 507 4910 1802
Fragments arranged by size:
4910 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2088 4666 5555
Size: 54 1425 50 275 18 258 2578 889
Cuts at: 5555 7184 8
Size: 1629 43
Fragments arranged by size:
2578 1629 1425 889 275 258 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4666 1537
Size: 275 2854 4090
Fragments arranged by size:
4090 2854 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2094 2358 2389 5149 1353
Size: 741 264 31 2760 3423
Fragments arranged by size:
3423 2760 741 264 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5806 6180 6326 387
Size: 1064 138 4217 374 146 1280
Fragments arranged by size:
4217 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2031 2507 2682 3297 3380 3687 3698
Size: 393 399 476 175 615 83 307 11
Cuts at: 3698 3913 4094 4425 4512 5282 5879 6666 6934
Size: 215 181 331 87 770 597 787 268
Cuts at: 6934 1239
Size: 1524
Fragments arranged by size:
1524 787 770 615 597 476 399 393
331 307 268 215 181 175 87 83
11
EcoO109I rG'GnC_Cy
Cuts at: 2819 2819
Size: 7219
EcoRI G'AATT_C
Cuts at: 2870 4528 4906 2870
Size: 1658 378 5183
Fragments arranged by size:
5183 1658 378
EcoRV GAT'ATC
Cuts at: 2893 2893
Size: 7219
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2129 4315 2129
Size: 2186 5033
FspI TGC'GCA
Cuts at: 5725 5725
Size: 7219
HaeII r_GCGC'y
Cuts at: 1678 6366 1678
Size: 4688 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2165 2197 2414 2446 2474
Size: 32 135 32 593 32 217 32 28
Cuts at: 2474 2506 2549 2581 3139 3171 3387 3419 3724
Size: 32 43 32 558 32 216 32 305
Cuts at: 3724 3756 3802 3834 4359 4391 5860 5892 5934
Size: 32 46 32 525 32 1469 32 42
Cuts at: 5934 5966 1373
Size: 32 2626
Fragments arranged by size:
2626 1469 593 558 525 305 217 216
135 46 43 42 32 32 32 32
32 32 32 32 32 32 32 32
32 28
HincII GTy'rAC
Cuts at: 669 1657 1938 2184 3524 4563 4809 669
Size: 988 281 246 1340 1039 246 3079
Fragments arranged by size:
3079 1340 1039 988 281 246 246
HindIII A'AGCT_T
Cuts at: 748 3232 748
Size: 2484 4735
HpaI GTT'AAC
Cuts at: 4809 4809
Size: 7219
KpnI G_GTAC'C
Cuts at: 2200 2683 4544 2200
Size: 483 1861 4875
Fragments arranged by size:
4875 1861 483
MfeI C'AATT_G
Cuts at: 2349 3465 3501 4818 2349
Size: 1116 36 1317 4750
Fragments arranged by size:
4750 1317 1116 36
MluI A'CGCG_T
Cuts at: 7095 7095
Size: 7219
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3867 4083 6333 6517 1305
Size: 2562 216 2250 184 2007
Fragments arranged by size:
2562 2250 2007 216 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5397
Fragments arranged by size:
5397 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2075 3896 5177 5536 5695 518
Size: 648 909 1821 1281 359 159 2042
Fragments arranged by size:
2042 1821 1281 909 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2012 2024 2031 4214 4635 5312 6460 6705
Size: 406 12 7 2183 421 677 1148 245
Cuts at: 6705 1606
Size: 2120
Fragments arranged by size:
2183 2120 1148 677 421 406 245 12
7
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6667
NdeI CA'TA_TG
Cuts at: 387 3318 387
Size: 2931 4288
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7219
NotI GC'GGCC_GC
Cuts at: 4666 4666
Size: 7219
NspI r_CATG'y
Cuts at: 1132 1229 2167 3478 3886 3979 4577 6122 1132
Size: 97 938 1311 408 93 598 1545 2229
Fragments arranged by size:
2229 1545 1311 938 598 408 97 93
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7219
PciI A'CATG_T
Cuts at: 3474 6118 3474
Size: 2644 4575
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7219
PmeI GTTT'AAAC
Cuts at: 4523 4523
Size: 7219
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5288 5320 6801 6833 5288
Size: 32 1481 32 5674
Fragments arranged by size:
5674 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2819 2819
Size: 7219
PshAI GACnn'nnGTC
Cuts at: 1613 3361 1613
Size: 1748 5471
PsiI TTA'TAA
Cuts at: 4789 4789
Size: 7219
PstI C_TGCA'G
Cuts at: 830 3687 4060 4571 830
Size: 2857 373 511 3478
Fragments arranged by size:
3478 2857 511 373
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7219
PvuII CAG'CTG
Cuts at: 4214 4214
Size: 7219
SacII CC_GC'GG
Cuts at: 2025 2025
Size: 7219
SalI G'TCGA_C
Cuts at: 1936 2182 3522 4561 1936
Size: 246 1340 1039 4594
Fragments arranged by size:
4594 1340 1039 246
SbfI CC_TGCA'GG
Cuts at: 4571 4571
Size: 7219
ScaI AGT'ACT
Cuts at: 5467 7179 5467
Size: 1712 5507
SfcI C'TryA_G
Cuts at: 826 1045 3657 3683 3744 4056 4567 5702 6383
Size: 219 2612 26 61 312 511 1135 681
Cuts at: 6383 6574 826
Size: 191 1471
Fragments arranged by size:
2612 1471 1135 681 511 312 219 191
61 26
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7219
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7219
SmaI CCC'GGG
Cuts at: 1872 4546 1872
Size: 2674 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5323 6224 6486 820
Size: 197 926 3380 901 262 1553
Fragments arranged by size:
3380 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5010 493
Size: 4517 2702
SpeI A'CTAG_T
Cuts at: 152 3257 152
Size: 3105 4114
SphI G_CATG'C
Cuts at: 1132 4577 1132
Size: 3445 3774
SspI AAT'ATT
Cuts at: 5 52 2416 2950 5143 5
Size: 47 2364 534 2193 2081
Fragments arranged by size:
2364 2193 2081 534 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7219
StyI C'CwwG_G
Cuts at: 513 1065 4658 513
Size: 552 3593 3074
Fragments arranged by size:
3593 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3036 3639 5246 5263 5416 5601 6046
Size: 1255 1456 603 1607 17 153 185 445
Cuts at: 6046 325
Size: 1498
Fragments arranged by size:
1607 1498 1456 1255 603 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4022 5465 7177
Size: 274 80 33 51 1443 2045 1443 1712
Cuts at: 7177 96
Size: 138
Fragments arranged by size:
2045 1712 1443 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2877 2909 7179 7211 2877
Size: 32 4270 32 2885
Fragments arranged by size:
4270 2885 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2606 1316
Size: 1290 5929
XbaI T'CTAG_A
Cuts at: 4334 4393 4555 4334
Size: 59 162 6998
Fragments arranged by size:
6998 162 59
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7219
XmaI C'CCGG_G
Cuts at: 1870 4544 1870
Size: 2674 4545
XmnI GAAnn'nnTTC
Cuts at: 2520 5348 2520
Size: 2828 4391
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5027 276
Size: 53 83 186 936 3493 2468
Fragments arranged by size:
3493 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI