Restriction map for
pFN21AB7325
(Circular) (Six-base) MAPSORT of: pFN21AB7325.seq Check: 3436 from: 1 to: 9652
pFN21AB7325 9652 bp
With 182 enzymes: SgfI *
March 9, 2009 17:03 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7462 278
Size: 53 83 186 936 5926 2468
Fragments arranged by size:
5926 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2892 5673 5770 6995 1937
Size: 955 2781 97 1225 4594
Fragments arranged by size:
4594 2781 1225 955 97
Acc65I G'GTAC_C
Cuts at: 6973 6973
Size: 9652
AclI AA'CG_TT
Cuts at: 1563 2602 7779 8152 1563
Size: 1039 5177 373 3063
Fragments arranged by size:
5177 3063 1039 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2933 3681 3690 4245 4671 4768 4821 5180
Size: 1301 748 9 555 426 97 53 359
Cuts at: 5180 5561 6546 6560 6662 7715 9099 9367 1632
Size: 381 985 14 102 1053 1384 268 1917
Fragments arranged by size:
1917 1384 1301 1053 985 748 555 426
381 359 268 102 97 53 14 9
AfeI AGC'GCT
Cuts at: 4154 4154
Size: 9652
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9455
AflIII A'CryG_T
Cuts at: 1203 5425 8551 9528 1203
Size: 4222 3126 977 1327
Fragments arranged by size:
4222 3126 1327 977
AgeI A'CCGG_T
Cuts at: 2146 8483 2146
Size: 6337 3315
AhdI GACnn_n'nnGTC
Cuts at: 8381 8381
Size: 9652
AleI CACnn'nnGTG
Cuts at: 1166 3150 6353 6809 1166
Size: 1984 3203 456 4009
Fragments arranged by size:
4009 3203 1984 456
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3379 1184
Size: 2195 7457
AlwNI CAG_nnn'CTG
Cuts at: 2092 2485 2915 3232 4268 4456 4479 6472 6823
Size: 393 430 317 1036 188 23 1993 351
Cuts at: 6823 7395 8967 2092
Size: 572 1572 2777
Fragments arranged by size:
2777 1993 1572 1036 572 430 393 351
317 188 23
ApaI G_GGCC'C
Cuts at: 2026 4026 2026
Size: 2000 7652
ApaLI G'TGCA_C
Cuts at: 2843 4388 7709 8865 2843
Size: 1545 3321 1156 3630
Fragments arranged by size:
3630 3321 1545 1156
ApoI r'AATT_y
Cuts at: 1493 2802 3041 5401 5732 5827 6723 6870 6961
Size: 1309 239 2360 331 95 896 147 91
Cuts at: 6961 7187 7339 9448 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2360 2109 1697 1309 896 331 239 226
152 147 95 91
AseI AT'TA_AT
Cuts at: 160 1032 5834 8206 160
Size: 872 4802 2372 1606
Fragments arranged by size:
4802 2372 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9652
AvaI C'yCGr_G
Cuts at: 1870 1943 2027 3390 4186 6977 1870
Size: 73 84 1363 796 2791 4545
Fragments arranged by size:
4545 2791 1363 796 84 73
BamHI G'GATC_C
Cuts at: 5361 5646 6982 7349 5361
Size: 285 1336 367 7664
Fragments arranged by size:
7664 1336 367 285
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3314 6973 8428 618
Size: 325 217 627 1527 3659 1455 1842
Fragments arranged by size:
3659 1842 1527 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2026 2160 2467 4026 4193 6971 1391
Size: 635 134 307 1559 167 2778 4072
Fragments arranged by size:
4072 2778 1559 635 307 167 134
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4228 4902 5745 928
Size: 971 2329 674 843 4835
Fragments arranged by size:
4835 2329 971 843 674
BbvCI CC'TCA_GC
Cuts at: 3476 4118 3476
Size: 642 9010
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3191 3225 6506 6540 7843 7877 1903
Size: 34 1254 34 3281 34 1303 34 3678
Fragments arranged by size:
3678 3281 1303 1254 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7544 8754 7544
Size: 1210 8442
BclI T'GATC_A
Cuts at: 1548 2830 5940 1548
Size: 1282 3110 5260
Fragments arranged by size:
5260 3110 1282
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2096 3186 8263
Size: 107 122 71 631 605 424 1090 5077
Cuts at: 8263 136
Size: 1525
Fragments arranged by size:
5077 1525 1090 631 605 424 122 107
71
BglII A'GATC_T
Cuts at: 5103 5809 9647 5103
Size: 706 3838 5108
Fragments arranged by size:
5108 3838 706
BlpI GC'TnA_GC
Cuts at: 2592 3225 7069 2592
Size: 633 3844 5175
Fragments arranged by size:
5175 3844 633
Bme1580I G_kGCm'C
Cuts at: 1790 2026 2847 4026 4168 4392 7713 8869 1790
Size: 236 821 1179 142 224 3321 1156 2573
Fragments arranged by size:
3321 2573 1179 1156 821 236 224 142
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9652
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3711 4237 4710
Size: 416 53 83 399 24 2294 526 473
Cuts at: 4710 6070 7383 8341 442
Size: 1360 1313 958 1753
Fragments arranged by size:
2294 1753 1360 1313 958 526 473 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 6617 1056
Size: 5561 4091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2114 4177 4501 5495 6069 6458 8312 1239
Size: 875 2063 324 994 574 389 1854 2579
Fragments arranged by size:
2579 2063 1854 994 875 574 389 324
Bpu10I CC'TnA_GC
Cuts at: 2466 3176 3476 4118 2466
Size: 710 300 642 8000
Fragments arranged by size:
8000 710 642 300
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2172 4976 7777 8642 8940 2172
Size: 2804 2801 865 298 2884
Fragments arranged by size:
2884 2804 2801 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3163 8315 882
Size: 617 1664 5152 2219
Fragments arranged by size:
5152 2219 1664 617
BsaAI yAC'GTr
Cuts at: 493 1204 2392 7443 493
Size: 711 1188 5051 2702
Fragments arranged by size:
5051 2702 1188 711
BsaBI GATnn'nnATC
Cuts at: 9646 9646
Size: 9652
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3451 4004 4233 7459
Size: 53 83 186 936 1918 553 229 3226
Cuts at: 7459 7841 8476 275
Size: 382 635 1451
Fragments arranged by size:
3226 1918 1451 936 635 553 382 229
186 83 53
BsaWI w'CCGG_w
Cuts at: 2050 2146 8085 8483 8757 8904 9315 2050
Size: 96 5939 398 274 147 411 2387
Fragments arranged by size:
5939 2387 411 398 274 147 96
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3362 3392 6576 6606 6614
Size: 30 165 30 1959 30 3184 30 8
Cuts at: 6614 6644 1178
Size: 30 4186
Fragments arranged by size:
4186 3184 1959 165 30 30 30 30
30 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2486 2722 5039 5728 5906 1186
Size: 1300 236 2317 689 178 4932
Fragments arranged by size:
4932 2317 1300 689 236 178
BseYI C'CCAG_C
Cuts at: 2009 3594 3627 8855 9351 2009
Size: 1585 33 5228 496 2310
Fragments arranged by size:
5228 2310 1585 496 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2414 3153 3236 3845 3917 4032 5033
Size: 573 678 739 83 609 72 115 1001
Cuts at: 5033 5987 1163
Size: 954 4828
Fragments arranged by size:
4828 1001 954 739 678 609 573 115
83 72
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2038 2132 3989 7102 7863 8891
Size: 275 186 37 94 1857 3113 761 1028
Cuts at: 8891 1540
Size: 2301
Fragments arranged by size:
3113 2301 1857 1028 761 275 186 94
37
BsiHKAI G_wGCw'C
Cuts at: 2847 3147 4142 4227 4392 4430 5298 7713 7798
Size: 300 995 85 165 38 868 2415 85
Cuts at: 7798 8869 2847
Size: 1071 3630
Fragments arranged by size:
3630 2415 1071 995 868 300 165 85
85 38
BsmI GAATG_Cn'
Cuts at: 7163 7256 7163
Size: 93 9559
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2026 2160 2467 2847 3147 4026 4142
Size: 399 236 134 307 380 300 879 116
Cuts at: 4142 4168 4193 4227 4392 4430 5298 6971 7713
Size: 26 25 34 165 38 868 1673 742
Cuts at: 7713 7798 8869 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1673 1071 879 868 742 399 380
307 300 236 165 134 116 85 38
34 26 25
BspEI T'CCGG_A
Cuts at: 2050 9315 2050
Size: 7265 2387
BspHI T'CATG_A
Cuts at: 2440 6340 7541 9271 2440
Size: 3900 1201 1730 2821
Fragments arranged by size:
3900 2821 1730 1201
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2133 3204 5364 6072 7007 844
Size: 1289 1071 2160 708 935 3489
Fragments arranged by size:
3489 2160 1289 1071 935 708
BsrBI CCG'CTC
Cuts at: 1394 1823 3976 3985 7539 1394
Size: 429 2153 9 3554 3507
Fragments arranged by size:
3554 3507 2153 429 9
BsrDI GCAATG_nn'
Cuts at: 65 1248 3099 4342 8147 8321 65
Size: 1183 1851 1243 3805 174 1396
Fragments arranged by size:
3805 1851 1396 1243 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2146 3708 3885 3988 4060 4181 8296
Size: 110 336 1562 177 103 72 121 4115
Cuts at: 8296 8483 1700
Size: 187 2869
Fragments arranged by size:
4115 2869 1562 336 187 177 121 110
103 72
BsrGI T'GTAC_A
Cuts at: 96 6915 96
Size: 6819 2833
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9652
BssSI C'ACGA_G
Cuts at: 2078 3980 7712 8724 2078
Size: 1902 3732 1012 3006
Fragments arranged by size:
3732 3006 1902 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5044 794
Size: 4250 5402
BstBI TT'CG_AA
Cuts at: 8456 8456
Size: 9652
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9652
BstXI CCAn_nnnn'nTGG
Cuts at: 3127 3127
Size: 9652
BstYI r'GATC_y
Cuts at: 1972 4777 4845 4870 5103 5361 5646 5809 6736
Size: 2805 68 25 233 258 285 163 927
Cuts at: 6736 6982 7349 7734 7751 9203 9289 9301 9647
Size: 246 367 385 17 1452 86 12 346
Cuts at: 9647 1972
Size: 1977
Fragments arranged by size:
2805 1977 1452 927 385 367 346 285
258 246 233 163 86 68 25 17
12
Bsu36I CC'TnA_GG
Cuts at: 2734 2734
Size: 9652
BtgI C'CryG_G
Cuts at: 513 1065 2004 2017 4824 6580 513
Size: 552 939 13 2807 1756 3585
Fragments arranged by size:
3585 2807 1756 939 552 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4212 487
Size: 683 56 2986 5927
Fragments arranged by size:
5927 2986 683 56
BtsI GCAGTG_nn'
Cuts at: 2872 3861 3917 4713 5523 6111 6534 6718 7168
Size: 989 56 796 810 588 423 184 450
Cuts at: 7168 7962 7982 2872
Size: 794 20 4542
Fragments arranged by size:
4542 989 810 796 794 588 450 423
184 56 20
ClaI AT'CG_AT
Cuts at: 1554 5133 5452 1554
Size: 3579 319 5754
Fragments arranged by size:
5754 3579 319
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5361 5396 6100 6135 529
Size: 35 4797 35 704 35 4046
Fragments arranged by size:
4797 4046 704 35 35 35
DraI TTT'AAA
Cuts at: 5230 5406 6956 5230
Size: 176 1550 7926
Fragments arranged by size:
7926 1550 176
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3922 8659 809
Size: 507 2606 4737 1802
Fragments arranged by size:
4737 2606 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2035 2129 2343
Size: 54 1425 50 275 18 205 94 214
Cuts at: 2343 3879 3986 4029 4158 6217 7099 7988 9617
Size: 1536 107 43 129 2059 882 889 1629
Cuts at: 9617 8
Size: 43
Fragments arranged by size:
2059 1629 1536 1425 889 882 275 214
205 129 107 94 54 50 43 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2035 2129 3986 7099 1537
Size: 275 223 94 1857 3113 4090
Fragments arranged by size:
4090 3113 1857 275 223 94
EarI CTCTTCn'nnn_
Cuts at: 1353 2502 2550 2562 4418 4787 7582 1353
Size: 1149 48 12 1856 369 2795 3423
Fragments arranged by size:
3423 2795 1856 1149 369 48 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8239 8613 8759 387
Size: 1064 138 6650 374 146 1280
Fragments arranged by size:
6650 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 2933 3681 3690 4177 4245 4501
Size: 393 482 819 748 9 487 68 256
Cuts at: 4501 4671 4768 4821 5180 5495 5561 6069 6458
Size: 170 97 53 359 315 66 508 389
Cuts at: 6458 6546 6560 6662 7715 8312 9099 9367 1239
Size: 88 14 102 1053 597 787 268 1524
Fragments arranged by size:
1524 1053 819 787 748 597 508 487
482 393 389 359 315 268 256 170
102 97 88 68 66 53 14 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2737 4031 5891 2737
Size: 1294 1860 6498
Fragments arranged by size:
6498 1860 1294
EcoO109I rG'GnC_Cy
Cuts at: 2022 2023 2089 2650 2944 3188 3277 4023 2022
Size: 1 66 561 294 244 89 746 7651
Fragments arranged by size:
7651 746 561 294 244 89 66 1
EcoRI G'AATT_C
Cuts at: 3041 6961 7339 3041
Size: 3920 378 5354
Fragments arranged by size:
5354 3920 378
EcoRV GAT'ATC
Cuts at: 6147 6147
Size: 9652
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5185 5893 5185
Size: 708 8944
FspI TGC'GCA
Cuts at: 8158 8158
Size: 9652
HaeII r_GCGC'y
Cuts at: 1678 3352 4156 6652 8799 1678
Size: 1674 804 2496 2147 2531
Fragments arranged by size:
2531 2496 2147 1674 804
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2624 2656 2815 2847 3801
Size: 32 135 32 1052 32 159 32 954
Cuts at: 3801 3833 4455 4487 4606 4638 4801 4833 6131
Size: 32 622 32 119 32 163 32 1298
Cuts at: 6131 6163 6614 6646 8293 8325 8367 8399 1373
Size: 32 451 32 1647 32 42 32 2626
Fragments arranged by size:
2626 1647 1298 1052 954 622 451 163
159 135 119 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4561 5771 6996 7242 669
Size: 988 281 2623 1210 1225 246 3079
Fragments arranged by size:
3079 2623 1225 1210 988 281 246
HindIII A'AGCT_T
Cuts at: 748 6510 748
Size: 5762 3890
HpaI GTT'AAC
Cuts at: 7242 7242
Size: 9652
KpnI G_GTAC'C
Cuts at: 6977 6977
Size: 9652
MfeI C'AATT_G
Cuts at: 7251 7251
Size: 9652
MluI A'CGCG_T
Cuts at: 9528 9528
Size: 9652
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2469 3816 5881 8766 8950 1305
Size: 1164 1347 2065 2885 184 2007
Fragments arranged by size:
2885 2065 2007 1347 1164 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2345 4031 6219 10
Size: 54 1425 343 513 1686 2188 3443
Fragments arranged by size:
3443 2188 1686 1425 513 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3072 3125 3137 3150 3197 3821 4359
Size: 648 1906 53 12 13 47 624 538
Cuts at: 4359 4406 5093 5205 6353 6775 6809 7610 7969
Size: 47 687 112 1148 422 34 801 359
Cuts at: 7969 8128 518
Size: 159 2042
Fragments arranged by size:
2042 1906 1148 801 687 648 624 538
422 359 159 112 53 47 47 34
13 12
MspA1I CmG'CkG
Cuts at: 1606 3533 5328 6047 6820 7068 7745 8893 9138
Size: 1927 1795 719 773 248 677 1148 245
Cuts at: 9138 1606
Size: 2120
Fragments arranged by size:
2120 1927 1795 1148 773 719 677 248
245
NaeI GCC'GGC
Cuts at: 3887 3990 4062 3887
Size: 103 72 9477
Fragments arranged by size:
9477 103 72
NcoI C'CATG_G
Cuts at: 513 1065 2004 4824 6580 513
Size: 552 939 2820 1756 3585
Fragments arranged by size:
3585 2820 1756 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9652
NgoMIV G'CCGG_C
Cuts at: 3885 3988 4060 3885
Size: 103 72 9477
Fragments arranged by size:
9477 103 72
NheI G'CTAG_C
Cuts at: 1052 6613 1052
Size: 5561 4091
NotI GC'GGCC_GC
Cuts at: 7099 7099
Size: 9652
NspI r_CATG'y
Cuts at: 1132 1229 4919 5204 7010 8555 1132
Size: 97 3690 285 1806 1545 2229
Fragments arranged by size:
3690 2229 1806 1545 285 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9652
PciI A'CATG_T
Cuts at: 8551 8551
Size: 9652
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4774 5998 1108
Size: 3666 1224 4762
Fragments arranged by size:
4762 3666 1224
PmeI GTTT'AAAC
Cuts at: 6956 6956
Size: 9652
PmlI CAC'GTG
Cuts at: 2392 2392
Size: 9652
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7721 7753 9234 9266 7721
Size: 32 1481 32 8107
Fragments arranged by size:
8107 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2650 2944 3277 2650
Size: 294 333 9025
Fragments arranged by size:
9025 333 294
PshAI GACnn'nnGTC
Cuts at: 1613 6482 1613
Size: 4869 4783
PsiI TTA'TAA
Cuts at: 3423 3620 6876 7222 3423
Size: 197 3256 346 5853
Fragments arranged by size:
5853 3256 346 197
PspOMI G'GGCC_C
Cuts at: 2022 4022 2022
Size: 2000 7652
PstI C_TGCA'G
Cuts at: 830 6335 6468 7004 830
Size: 5505 133 536 3478
Fragments arranged by size:
5505 3478 536 133
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9652
PvuII CAG'CTG
Cuts at: 3533 6047 6820 3533
Size: 2514 773 6365
Fragments arranged by size:
6365 2514 773
RsrII CG'GwC_CG
Cuts at: 2144 2144
Size: 9652
SalI G'TCGA_C
Cuts at: 1936 5769 6994 1936
Size: 3833 1225 4594
Fragments arranged by size:
4594 3833 1225
SapI GCTCTTCn'nnn_
Cuts at: 2502 4418 2502
Size: 1916 7736
SbfI CC_TGCA'GG
Cuts at: 7004 7004
Size: 9652
ScaI AGT'ACT
Cuts at: 4415 6329 7900 9612 4415
Size: 1914 1571 1712 4455
Fragments arranged by size:
4455 1914 1712 1571
SfcI C'TryA_G
Cuts at: 826 1045 2084 3334 6135 6331 6464 6922 6937
Size: 219 1039 1250 2801 196 133 458 15
Cuts at: 6937 7000 8135 8816 9007 826
Size: 63 1135 681 191 1471
Fragments arranged by size:
2801 1471 1250 1135 1039 681 458 219
196 191 133 63 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 2096 2096
Size: 9652
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9652
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9652
SmaI CCC'GGG
Cuts at: 1872 2029 4188 6979 1872
Size: 157 2159 2791 4545
Fragments arranged by size:
4545 2791 2159 157
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2187 4991 7756 8657 8919 820
Size: 197 926 244 2804 2765 901 262 1553
Fragments arranged by size:
2804 2765 1553 926 901 262 244 197
SnaBI TAC'GTA
Cuts at: 493 7443 493
Size: 6950 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9652
SphI G_CATG'C
Cuts at: 1132 4919 7010 1132
Size: 3787 2091 3774
Fragments arranged by size:
3787 3774 2091
SrfI GCCC'GGGC
Cuts at: 4188 4188
Size: 9652
SspI AAT'ATT
Cuts at: 5 52 6756 7576 5
Size: 47 6704 820 2081
Fragments arranged by size:
6704 2081 820 47
StuI AGG'CCT
Cuts at: 1517 2193 3240 5081 1517
Size: 676 1047 1841 6088
Fragments arranged by size:
6088 1841 1047 676
StyI C'CwwG_G
Cuts at: 513 1065 2004 3481 4032 4741 4824 6580 7091
Size: 552 939 1477 551 709 83 1756 511
Cuts at: 7091 513
Size: 3074
Fragments arranged by size:
3074 1756 1477 939 709 552 551 511
83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4056 4126 7679 7696 7849 8034 8479
Size: 1255 2476 70 3553 17 153 185 445
Cuts at: 8479 325
Size: 1498
Fragments arranged by size:
3553 2476 1498 1255 445 185 153 70
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4413 5255 6327
Size: 274 80 33 51 1443 2436 842 1072
Cuts at: 6327 6460 6915 7898 9610 96
Size: 133 455 983 1712 138
Fragments arranged by size:
2436 1712 1443 1072 983 842 455 274
138 133 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6576 6608 9612 9644 6576
Size: 32 3004 32 6584
Fragments arranged by size:
6584 3004 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4469 1316
Size: 3153 6499
XbaI T'CTAG_A
Cuts at: 6988 6988
Size: 9652
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5470 5470
Size: 9652
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9652
XmaI C'CCGG_G
Cuts at: 1870 2027 4186 6977 1870
Size: 157 2159 2791 4545
Fragments arranged by size:
4545 2791 2159 157
XmnI GAAnn'nnTTC
Cuts at: 7781 7781
Size: 9652
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7460 276
Size: 53 83 186 936 5926 2468
Fragments arranged by size:
5926 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII RsrII SalI SapI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI FseI FspAI KasI
NarI NruI NsiI PacI PfoI PspXI PsrI SacI
SacII SanDI SexAI SfoI SwaI