Restriction map for
pFN21AB7323
(Circular) (Six-base) MAPSORT of: pFN21AB7323.seq Check: 775 from: 1 to: 10567
pFN21AB7323 10567 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2689 3092 3330 5300 5473 2689
Size: 403 238 1970 173 7783
Fragments arranged by size:
7783 1970 403 238 173
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8377 278
Size: 53 83 186 936 6841 2468
Fragments arranged by size:
6841 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 7910 1937
Size: 5973 4594
Acc65I G'GTAC_C
Cuts at: 7888 7888
Size: 10567
AclI AA'CG_TT
Cuts at: 1563 8694 9067 1563
Size: 7131 373 3063
Fragments arranged by size:
7131 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2479 3419 4625 5055 5123 5380 6224 7515
Size: 847 940 1206 430 68 257 844 1291
Cuts at: 7515 8630 10014 10282 1632
Size: 1115 1384 268 1917
Fragments arranged by size:
1917 1384 1291 1206 1115 940 847 844
430 268 257 68
AfeI AGC'GCT
Cuts at: 3746 3746
Size: 10567
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10370
AflIII A'CryG_T
Cuts at: 1203 4809 9466 10443 1203
Size: 3606 4657 977 1327
Fragments arranged by size:
4657 3606 1327 977
AgeI A'CCGG_T
Cuts at: 9398 9398
Size: 10567
AhdI GACnn_n'nnGTC
Cuts at: 2077 9296 2077
Size: 7219 3348
AleI CACnn'nnGTG
Cuts at: 1166 5483 6429 6653 1166
Size: 4317 946 224 5080
Fragments arranged by size:
5080 4317 946 224
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4217 5519 1184
Size: 3033 1302 6232
Fragments arranged by size:
6232 3033 1302
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2435 2467 4373 4405 4556 4588 4646 4678 2435
Size: 32 1906 32 151 32 58 32 8324
Fragments arranged by size:
8324 1906 151 58 32 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 5569 6772 7528 8310 9882 5569
Size: 1203 756 782 1572 6254
Fragments arranged by size:
6254 1572 1203 782 756
ApaI G_GGCC'C
Cuts at: 2015 4153 4257 5278 6606 7791 7805 7851 2015
Size: 2138 104 1021 1328 1185 14 46 4731
Fragments arranged by size:
4731 2138 1328 1185 1021 104 46 14
ApaLI G'TGCA_C
Cuts at: 3827 3868 8624 9780 3827
Size: 41 4756 1156 4614
Fragments arranged by size:
4756 4614 1156 41
ApoI r'AATT_y
Cuts at: 1493 4457 7103 7876 8102 8254 10363 1493
Size: 2964 2646 773 226 152 2109 1697
Fragments arranged by size:
2964 2646 2109 1697 773 226 152
AseI AT'TA_AT
Cuts at: 160 1032 9121 160
Size: 872 8089 1606
Fragments arranged by size:
8089 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10567
AvaI C'yCGr_G
Cuts at: 1870 1943 2404 2872 3088 3137 3347 5270 5304
Size: 73 461 468 216 49 210 1923 34
Cuts at: 5304 5454 6243 6477 6605 6746 6855 7892 1870
Size: 150 789 234 128 141 109 1037 4545
Fragments arranged by size:
4545 1923 1037 789 468 461 234 216
210 150 141 128 109 73 49 34
BamHI G'GATC_C
Cuts at: 3177 7897 8264 3177
Size: 4720 367 5480
Fragments arranged by size:
5480 4720 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2021 2570 2701 4318 5124
Size: 325 217 627 234 549 131 1617 806
Cuts at: 5124 5979 6423 7212 7218 7888 9343 618
Size: 855 444 789 6 670 1455 1842
Fragments arranged by size:
1842 1617 1455 855 806 789 670 627
549 444 325 234 217 131 6
BanII G_rGCy'C
Cuts at: 1391 2015 2136 2397 2826 3444 3489 4153 4257
Size: 624 121 261 429 618 45 664 104
Cuts at: 4257 4488 4517 4842 5278 5864 6099 6606 6836
Size: 231 29 325 436 586 235 507 230
Cuts at: 6836 6856 7035 7317 7791 7805 7851 7886 1391
Size: 20 179 282 474 14 46 35 4072
Fragments arranged by size:
4072 664 624 618 586 507 474 436
429 325 282 261 235 231 230 179
121 104 46 45 35 29 20 14
BbeI G_GCGC'C
Cuts at: 4322 7222 4322
Size: 2900 7667
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3693 3963 4752 5061 928
Size: 971 1794 270 789 309 6434
Fragments arranged by size:
6434 1794 971 789 309 270
BbvCI CC'TCA_GC
Cuts at: 4001 7020 4001
Size: 3019 7548
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2933 2967 8758 8792 1903
Size: 34 50 34 912 34 5791 34 3678
Fragments arranged by size:
5791 3678 912 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7195 8459 9669 7195
Size: 1264 1210 8093
Fragments arranged by size:
8093 1264 1210
BclI T'GATC_A
Cuts at: 1548 3837 1548
Size: 2289 8278
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2019 3976 5018
Size: 107 122 71 631 605 347 1957 1042
Cuts at: 5018 5273 6169 7490 7846 9178 136
Size: 255 896 1321 356 1332 1525
Fragments arranged by size:
1957 1525 1332 1321 1042 896 631 605
356 347 255 122 107 71
BglII A'GATC_T
Cuts at: 10562 10562
Size: 10567
BlpI GC'TnA_GC
Cuts at: 4859 6962 7607 7984 4859
Size: 2103 645 377 7442
Fragments arranged by size:
7442 2103 645 377
Bme1580I G_kGCm'C
Cuts at: 1790 2015 2024 2706 3831 3872 4153 4160 4257
Size: 225 9 682 1125 41 281 7 97
Cuts at: 4257 5127 5278 6477 6606 6660 6754 7791 7805
Size: 870 151 1199 129 54 94 1037 14
Cuts at: 7805 7851 8628 9784 1790
Size: 46 777 1156 2573
Fragments arranged by size:
2573 1199 1156 1125 1037 870 777 682
281 225 151 129 97 94 54 46
41 14 9 7
BmgBI CAC'GTC
Cuts at: 1318 2440 3481 4810 5617 5749 7627 1318
Size: 1122 1041 1329 807 132 1878 4258
Fragments arranged by size:
4258 1878 1329 1122 1041 807 132
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2009 3937 4303
Size: 416 53 83 399 24 592 1928 366
Cuts at: 4303 5971 8298 9256 442
Size: 1668 2327 958 1753
Fragments arranged by size:
2327 1928 1753 1668 958 592 416 399
366 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2684 1056
Size: 1628 8939
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4261 4381 6505 6649 4261
Size: 120 2124 144 8179
Fragments arranged by size:
8179 2124 144 120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2775 3146 3448 3533 3617 4139 4671 4673
Size: 1536 371 302 85 84 522 532 2
Cuts at: 4673 4727 4739 5192 5204 6475 6977 7370 7412
Size: 54 12 453 12 1271 502 393 42
Cuts at: 7412 7616 9227 1239
Size: 204 1611 2579
Fragments arranged by size:
2579 1611 1536 1271 532 522 502 453
393 371 302 204 85 84 54 42
12 12 2
Bpu10I CC'TnA_GC
Cuts at: 3504 4001 4790 5858 7020 7703 3504
Size: 497 789 1068 1162 683 6368
Fragments arranged by size:
6368 1162 1068 789 683 497
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2156 2297 2810 3899 3968 4544 4784 5567 8692
Size: 141 513 1089 69 576 240 783 3125
Cuts at: 8692 9557 9855 2156
Size: 865 298 2868
Fragments arranged by size:
3125 2868 1089 865 783 576 513 298
240 141 69
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6449 9230 882
Size: 617 4950 2781 2219
Fragments arranged by size:
4950 2781 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4333 4465 5884 6502 8358 493
Size: 711 3129 132 1419 618 1856 2702
Fragments arranged by size:
3129 2702 1856 1419 711 618 132
BsaBI GATnn'nnATC
Cuts at: 10561 10561
Size: 10567
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3300 4319 6339 7219
Size: 53 83 186 936 1767 1019 2020 880
Cuts at: 7219 8374 8756 9391 275
Size: 1155 382 635 1451
Fragments arranged by size:
2020 1767 1451 1155 1019 936 880 635
382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5805 7422 9000 9398 9672 9819 10230 5805
Size: 1617 1578 398 274 147 411 6142
Fragments arranged by size:
6142 1617 1578 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2384 2414 2915 2945 4070
Size: 30 165 30 981 30 501 30 1125
Cuts at: 4070 4100 4370 4400 4646 4676 5846 5876 6050
Size: 30 270 30 246 30 1170 30 174
Cuts at: 6050 6080 7385 7415 7806 7836 1178
Size: 30 1305 30 391 30 3909
Fragments arranged by size:
3909 1305 1170 1125 981 501 391 270
246 174 165 30 30 30 30 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 2045 2069 2474 2860 2909 2992 3227
Size: 856 3 24 405 386 49 83 235
Cuts at: 3227 3230 3245 3248 3251 3301 3755 4661 4670
Size: 3 15 3 3 50 454 906 9
Cuts at: 4670 4736 5285 5483 5909 5924 5954 6625 6875
Size: 66 549 198 426 15 30 671 250
Cuts at: 6875 6887 6932 7037 7184 7427 7613 7750 1186
Size: 12 45 105 147 243 186 137 4003
Fragments arranged by size:
4003 906 856 671 549 454 426 405
386 250 243 235 198 186 147 137
105 83 66 50 49 45 30 24
15 15 12 9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2706 3488 3949 4774 5277 6268 6318 6691 6717
Size: 782 461 825 503 991 50 373 26
Cuts at: 6717 6721 6835 7547 9770 10266 2706
Size: 4 114 712 2223 496 3007
Fragments arranged by size:
3007 2223 991 825 782 712 503 496
461 373 114 50 26 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4088 4223 4469 6471 7559 1163
Size: 573 2352 135 246 2002 1088 4171
Fragments arranged by size:
4171 2352 2002 1088 573 246 135
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2820 8017 8778 9806 1540
Size: 275 186 819 5197 761 1028 2301
Fragments arranged by size:
5197 2301 1028 819 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3831 3872 4488 4842 5136 5864 6099 8628 8713
Size: 41 616 354 294 728 235 2529 85
Cuts at: 8713 9784 3831
Size: 1071 4614
Fragments arranged by size:
4614 2529 1071 728 616 354 294 235
85 41
BsmI GAATG_Cn'
Cuts at: 2951 8078 8171 2951
Size: 5127 93 5347
Fragments arranged by size:
5347 5127 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2651 6869 2651
Size: 4218 6349
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2024 2136 2397 2706 2826 3444
Size: 399 225 9 112 261 309 120 618
Cuts at: 3444 3489 3831 3872 4153 4160 4257 4488 4517
Size: 45 342 41 281 7 97 231 29
Cuts at: 4517 4842 5127 5136 5278 5864 6099 6477 6606
Size: 325 285 9 142 586 235 378 129
Cuts at: 6606 6660 6754 6836 6856 7035 7317 7791 7805
Size: 54 94 82 20 179 282 474 14
Cuts at: 7805 7851 7886 8628 8713 9784 1391
Size: 46 35 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 618 586 474 399 378
342 325 309 285 282 281 261 235
231 225 179 142 129 120 112 97
94 85 82 54 46 45 41 35
29 20 14 9 9 7
BspEI T'CCGG_A
Cuts at: 7422 10230 7422
Size: 2808 7759
BspHI T'CATG_A
Cuts at: 4959 8456 10186 4959
Size: 3497 1730 5340
Fragments arranged by size:
5340 3497 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2689 3092 3330 3861 4779 5300 5473 6037
Size: 1845 403 238 531 918 521 173 564
Cuts at: 6037 7368 7922 844
Size: 1331 554 3489
Fragments arranged by size:
3489 1845 1331 918 564 554 531 521
403 238 173
BsrBI CCG'CTC
Cuts at: 1394 1823 4365 5209 5920 6865 7174 8454 1394
Size: 429 2542 844 711 945 309 1280 3507
Fragments arranged by size:
3507 2542 1280 945 844 711 429 309
BsrDI GCAATG_nn'
Cuts at: 65 1248 2224 2517 3325 3568 9062 9236 65
Size: 1183 976 293 808 243 5494 174 1396
Fragments arranged by size:
5494 1396 1183 976 808 293 243 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4825 5647 7215 9211 9398 1700
Size: 110 3015 822 1568 1996 187 2869
Fragments arranged by size:
3015 2869 1996 1568 822 187 110
BsrGI T'GTAC_A
Cuts at: 96 7770 96
Size: 7674 2893
BssHII G'CGCG_C
Cuts at: 1925 4269 1925
Size: 2344 8223
BssSI C'ACGA_G
Cuts at: 6827 8627 9639 6827
Size: 1800 1012 7755
Fragments arranged by size:
7755 1800 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4771 5131 6502 794
Size: 3977 360 1371 4859
Fragments arranged by size:
4859 3977 1371 360
BstBI TT'CG_AA
Cuts at: 9371 9371
Size: 10567
BstEII G'GTnAC_C
Cuts at: 1184 2662 4336 1184
Size: 1478 1674 7415
Fragments arranged by size:
7415 1674 1478
BstXI CCAn_nnnn'nTGG
Cuts at: 3059 5161 5800 3059
Size: 2102 639 7826
Fragments arranged by size:
7826 2102 639
BstYI r'GATC_y
Cuts at: 1972 2245 2538 3177 3351 6942 7897 8264 8649
Size: 273 293 639 174 3591 955 367 385
Cuts at: 8649 8666 10118 10204 10216 10562 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
3591 1977 1452 955 639 385 367 346
293 273 174 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2507 6597 2507
Size: 4090 6477
BtgI C'CryG_G
Cuts at: 513 1065 2039 2619 4050 4073 5013 5334 7292
Size: 552 974 580 1431 23 940 321 1958
Cuts at: 7292 7470 513
Size: 178 3610
Fragments arranged by size:
3610 1958 1431 974 940 580 552 321
178 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 7108 487
Size: 683 56 5882 3946
Fragments arranged by size:
5882 3946 683 56
BtsI GCAGTG_nn'
Cuts at: 2281 4100 5767 8083 8877 8897 2281
Size: 1819 1667 2316 794 20 3951
Fragments arranged by size:
3951 2316 1819 1667 794 20
ClaI AT'CG_AT
Cuts at: 1554 3882 1554
Size: 2328 8239
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3308 3343 529
Size: 35 2744 35 7753
Fragments arranged by size:
7753 2744 35 35
DraI TTT'AAA
Cuts at: 7871 7871
Size: 10567
DraIII CAC_nnn'GTG
Cuts at: 3613 5131 5518 7158 3613
Size: 1518 387 1640 7022
Fragments arranged by size:
7022 1640 1518 387
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4052 9574 809
Size: 507 2736 5522 1802
Fragments arranged by size:
5522 2736 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2418 2817 4173
Size: 54 1425 50 275 18 588 399 1356
Cuts at: 4173 4827 5331 5416 5487 5694 5784 6866 7668
Size: 654 504 85 71 207 90 1082 802
Cuts at: 7668 8014 8903 10532 8
Size: 346 889 1629 43
Fragments arranged by size:
1629 1425 1356 1082 889 802 654 588
504 399 346 275 207 90 85 71
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2817 8014 1537
Size: 275 1005 5197 4090
Fragments arranged by size:
5197 4090 1005 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2235 2247 2963 2987 3204 3698 5003 5609
Size: 882 12 716 24 217 494 1305 606
Cuts at: 5609 6150 6200 6374 6419 8497 1353
Size: 541 50 174 45 2078 3423
Fragments arranged by size:
3423 2078 1305 882 716 606 541 494
217 174 50 45 24 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5354 6458 9154 9528 9674 387
Size: 1064 138 3765 1104 2696 374 146 1280
Fragments arranged by size:
3765 2696 1280 1104 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4486 4840 5862 6097 4486
Size: 354 1022 235 8956
Fragments arranged by size:
8956 1022 354 235
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2479 2775 3146 3419 3448 3533 3617
Size: 393 847 296 371 273 29 85 84
Cuts at: 3617 4139 4625 4671 4673 4727 4739 5055 5123
Size: 522 486 46 2 54 12 316 68
Cuts at: 5123 5192 5204 5380 6224 6475 6977 7370 7412
Size: 69 12 176 844 251 502 393 42
Cuts at: 7412 7515 7616 8630 9227 10014 10282 1239
Size: 103 101 1014 597 787 268 1524
Fragments arranged by size:
1524 1014 847 844 787 597 522 502
486 393 393 371 316 296 273 268
251 176 103 101 85 84 69 68
54 46 42 29 12 12 2
EcoNI CCTnn'n_nnAGG
Cuts at: 3088 3917 4232 5171 5240 6023 7532 7577 3088
Size: 829 315 939 69 783 1509 45 6078
Fragments arranged by size:
6078 1509 939 829 783 315 69 45
EcoO109I rG'GnC_Cy
Cuts at: 2011 2428 2750 3093 3473 3718 4123 4253 4254
Size: 417 322 343 380 245 405 130 1
Cuts at: 4254 4632 5020 5233 5274 6004 6367 6484 6602
Size: 378 388 213 41 730 363 117 118
Cuts at: 6602 6784 6886 7453 7582 7729 7801 7802 7847
Size: 182 102 567 129 147 72 1 45
Cuts at: 7847 7848 2011
Size: 1 4730
Fragments arranged by size:
4730 730 567 417 405 388 380 378
363 343 322 245 213 182 147 130
129 118 117 102 72 45 41 1
1 1
EcoRI G'AATT_C
Cuts at: 7876 8254 7876
Size: 378 10189
EcoRV GAT'ATC
Cuts at: 7864 7864
Size: 10567
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2851 2851
Size: 10567
FspI TGC'GCA
Cuts at: 9073 9073
Size: 10567
HaeII r_GCGC'y
Cuts at: 1678 2595 2916 3748 4322 7222 7752 9714 1678
Size: 917 321 832 574 2900 530 1962 2531
Fragments arranged by size:
2900 2531 1962 917 832 574 530 321
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2063 2095 2213 2245 2384
Size: 32 135 32 491 32 118 32 139
Cuts at: 2384 2416 3659 3691 5039 5071 5657 5689 6083
Size: 32 1243 32 1348 32 586 32 394
Cuts at: 6083 6095 6115 6127 7806 7838 9208 9240 9282
Size: 12 20 12 1679 32 1370 32 42
Cuts at: 9282 9314 1373
Size: 32 2626
Fragments arranged by size:
2626 1679 1370 1348 1243 586 491 394
139 135 118 42 32 32 32 32
32 32 32 32 32 32 32 20
12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 6052 6565 7825 7911 8157 669
Size: 988 281 4114 513 1260 86 246 3079
Fragments arranged by size:
4114 3079 1260 988 513 281 246 86
HindIII A'AGCT_T
Cuts at: 748 748
Size: 10567
HpaI GTT'AAC
Cuts at: 8157 8157
Size: 10567
KasI G'GCGC_C
Cuts at: 4318 7218 4318
Size: 2900 7667
KpnI G_GTAC'C
Cuts at: 7892 7892
Size: 10567
MfeI C'AATT_G
Cuts at: 8166 8166
Size: 10567
MluI A'CGCG_T
Cuts at: 10443 10443
Size: 10567
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2241 2880 7668 9681 9865 1305
Size: 936 639 4788 2013 184 2007
Fragments arranged by size:
4788 2013 2007 936 639 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5333 5489 10
Size: 54 1425 343 3501 156 5088
Fragments arranged by size:
5088 3501 1425 343 156 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3614 4961 5483 5985 6263 6429 6653
Size: 648 2448 1347 522 502 278 166 224
Cuts at: 6653 8525 8884 9043 518
Size: 1872 359 159 2042
Fragments arranged by size:
2448 2042 1872 1347 648 522 502 359
278 224 166 159
MspA1I CmG'CkG
Cuts at: 1606 2041 2621 2630 3343 3415 3595 4117 6202
Size: 435 580 9 713 72 180 522 2085
Cuts at: 6202 6289 6691 6721 6907 7269 7594 7983 8660
Size: 87 402 30 186 362 325 389 677
Cuts at: 8660 9808 10053 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 2085 1148 713 677 580 522 435
402 389 362 325 245 186 180 87
72 30 9
NaeI GCC'GGC
Cuts at: 4827 4827
Size: 10567
NarI GG'CG_CC
Cuts at: 4319 7219 4319
Size: 2900 7667
NcoI C'CATG_G
Cuts at: 513 1065 4073 7470 513
Size: 552 3008 3397 3610
Fragments arranged by size:
3610 3397 3008 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10567
NgoMIV G'CCGG_C
Cuts at: 4825 4825
Size: 10567
NheI G'CTAG_C
Cuts at: 1052 2680 1052
Size: 1628 8939
NotI GC'GGCC_GC
Cuts at: 8014 8014
Size: 10567
NspI r_CATG'y
Cuts at: 1132 1229 4789 4825 6328 6563 7925 9470 1132
Size: 97 3560 36 1503 235 1362 1545 2229
Fragments arranged by size:
3560 2229 1545 1503 1362 235 97 36
PasI CC'CwG_GG
Cuts at: 1348 2959 4153 7843 1348
Size: 1611 1194 3690 4072
Fragments arranged by size:
4072 3690 1611 1194
PciI A'CATG_T
Cuts at: 9466 9466
Size: 10567
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4524 5518 6358 1108
Size: 3416 994 840 5317
Fragments arranged by size:
5317 3416 994 840
PfoI T'CCnGG_A
Cuts at: 4716 5169 5181 6191 6945 4716
Size: 453 12 1010 754 8338
Fragments arranged by size:
8338 1010 754 453 12
PmeI GTTT'AAAC
Cuts at: 7871 7871
Size: 10567
PmlI CAC'GTG
Cuts at: 4465 6502 4465
Size: 2037 8530
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3518 3550 4646 4678 8636 8668 10149 10181 3518
Size: 32 1096 32 3958 32 1481 32 3904
Fragments arranged by size:
3958 3904 1481 1096 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3718 4632 6004 6367 7453 7582 7729 3718
Size: 914 1372 363 1086 129 147 6556
Fragments arranged by size:
6556 1372 1086 914 363 147 129
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10567
PsiI TTA'TAA
Cuts at: 8137 8137
Size: 10567
PspOMI G'GGCC_C
Cuts at: 2011 4149 4253 5274 6602 7787 7801 7847 2011
Size: 2138 104 1021 1328 1185 14 46 4731
Fragments arranged by size:
4731 2138 1328 1185 1021 104 46 14
PspXI vC'TCGA_Gb
Cuts at: 2872 2872
Size: 10567
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4871 4903 4871
Size: 32 10535
PstI C_TGCA'G
Cuts at: 830 2956 7683 7919 830
Size: 2126 4727 236 3478
Fragments arranged by size:
4727 3478 2126 236
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10567
PvuII CAG'CTG
Cuts at: 3595 4117 6202 6721 6907 7594 3595
Size: 522 2085 519 186 687 6568
Fragments arranged by size:
6568 2085 687 522 519 186
SacI G_AGCT'C
Cuts at: 4488 4842 5864 6099 4488
Size: 354 1022 235 8956
Fragments arranged by size:
8956 1022 354 235
SacII CC_GC'GG
Cuts at: 2042 2622 2042
Size: 580 9987
SalI G'TCGA_C
Cuts at: 1936 7909 1936
Size: 5973 4594
SanDI GG'GwC_CC
Cuts at: 4632 7729 4632
Size: 3097 7470
SapI GCTCTTCn'nnn_
Cuts at: 2963 6374 2963
Size: 3411 7156
SbfI CC_TGCA'GG
Cuts at: 7919 7919
Size: 10567
ScaI AGT'ACT
Cuts at: 4880 8815 10527 4880
Size: 3935 1712 4920
Fragments arranged by size:
4920 3935 1712
SexAI A'CCwGG_T
Cuts at: 4260 5514 4260
Size: 1254 9313
SfcI C'TryA_G
Cuts at: 826 1045 2652 2952 3590 5090 5820 5837 7679
Size: 219 1607 300 638 1500 730 17 1842
Cuts at: 7679 7915 9050 9731 9922 826
Size: 236 1135 681 191 1471
Fragments arranged by size:
1842 1607 1500 1471 1135 730 681 638
300 236 219 191 17
SfoI GGC'GCC
Cuts at: 4320 7220 4320
Size: 2900 7667
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10567
SgrAI Cr'CCGG_yG
Cuts at: 1700 7215 1700
Size: 5515 5052
SmaI CCC'GGG
Cuts at: 1872 5272 5306 6479 6748 7894 1872
Size: 3400 34 1173 269 1146 4545
Fragments arranged by size:
4545 3400 1173 1146 269 34
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2171 2312 2825 2872 3914 3983
Size: 197 926 228 141 513 47 1042 69
Cuts at: 3983 4559 4799 5582 8671 9572 9834 820
Size: 576 240 783 3089 901 262 1553
Fragments arranged by size:
3089 1553 1042 926 901 783 576 513
262 240 228 197 141 69 47
SnaBI TAC'GTA
Cuts at: 493 8358 493
Size: 7865 2702
SpeI A'CTAG_T
Cuts at: 152 6529 152
Size: 6377 4190
SphI G_CATG'C
Cuts at: 1132 6328 7925 1132
Size: 5196 1597 3774
Fragments arranged by size:
5196 3774 1597
SspI AAT'ATT
Cuts at: 5 52 6119 8491 5
Size: 47 6067 2372 2081
Fragments arranged by size:
6067 2372 2081 47
StuI AGG'CCT
Cuts at: 1517 2492 3923 5393 6296 1517
Size: 975 1431 1470 903 5788
Fragments arranged by size:
5788 1470 1431 975 903
StyI C'CwwG_G
Cuts at: 513 1065 2736 4073 4740 4923 6761 6798 7014
Size: 552 1671 1337 667 183 1838 37 216
Cuts at: 7014 7470 7533 8006 513
Size: 456 63 473 3074
Fragments arranged by size:
3074 1838 1671 1337 667 552 473 456
216 183 63 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2558 3568 4850 5525 5671 7874 8594
Size: 1255 978 1010 1282 675 146 2203 720
Cuts at: 8594 8611 8764 8949 9394 325
Size: 17 153 185 445 1498
Fragments arranged by size:
2203 1498 1282 1255 1010 978 720 675
445 185 153 146 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3383 4878 6573
Size: 274 80 33 51 1443 1406 1495 1695
Cuts at: 6573 7770 8813 10525 96
Size: 1197 1043 1712 138
Fragments arranged by size:
1712 1695 1495 1443 1406 1197 1043 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2168 2200 2915 2947 5203 5235 6068 6100 10527
Size: 32 715 32 2256 32 833 32 4427
Cuts at: 10527 10559 2168
Size: 32 2176
Fragments arranged by size:
4427 2256 2176 833 715 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5345 1316
Size: 4029 6538
XbaI T'CTAG_A
Cuts at: 7903 7903
Size: 10567
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3363 5566 3363
Size: 2203 8364
XhoI C'TCGA_G
Cuts at: 1943 2872 1943
Size: 929 9638
XmaI C'CCGG_G
Cuts at: 1870 5270 5304 6477 6746 7892 1870
Size: 3400 34 1173 269 1146 4545
Fragments arranged by size:
4545 3400 1173 1146 269 34
XmnI GAAnn'nnTTC
Cuts at: 4427 7364 8696 4427
Size: 2937 1332 6298
Fragments arranged by size:
6298 2937 1332
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8375 276
Size: 53 83 186 936 6841 2468
Fragments arranged by size:
6841 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BstZ17I FseI FspAI
NruI NsiI PacI RsrII SfiI SrfI SwaI