Restriction map for
pFN21AB7314
(Circular) (Six-base) MAPSORT of: pFN21AB7314.seq Check: 1112 from: 1 to: 7942
pFN21AB7314 7942 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5100 5100
Size: 7942
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5752 278
Size: 53 83 186 936 4216 2468
Fragments arranged by size:
4216 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2412 4449 5285 1937
Size: 475 2037 836 4594
Fragments arranged by size:
4594 2037 836 475
Acc65I G'GTAC_C
Cuts at: 5263 5263
Size: 7942
AclI AA'CG_TT
Cuts at: 1563 3142 6069 6442 1563
Size: 1579 2927 373 3063
Fragments arranged by size:
3063 2927 1579 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2277 4290 6005 7389 7657 1632
Size: 645 2013 1715 1384 268 1917
Fragments arranged by size:
2013 1917 1715 1384 645 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7745
AflIII A'CryG_T
Cuts at: 1203 6841 7818 1203
Size: 5638 977 1327
Fragments arranged by size:
5638 1327 977
AgeI A'CCGG_T
Cuts at: 6773 6773
Size: 7942
AhdI GACnn_n'nnGTC
Cuts at: 2545 6671 2545
Size: 4126 3816
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7942
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7942
AlwNI CAG_nnn'CTG
Cuts at: 4124 4858 5685 7257 4124
Size: 734 827 1572 4809
Fragments arranged by size:
4809 1572 827 734
ApaLI G'TGCA_C
Cuts at: 5999 7155 5999
Size: 1156 6786
ApoI r'AATT_y
Cuts at: 1493 2499 2613 3179 3417 3600 4156 5251 5477
Size: 1006 114 566 238 183 556 1095 226
Cuts at: 5477 5629 7738 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1095 1006 566 556 238 226
183 152 114
AseI AT'TA_AT
Cuts at: 160 1032 4163 6496 160
Size: 872 3131 2333 1606
Fragments arranged by size:
3131 2333 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7942
AvaI C'yCGr_G
Cuts at: 1870 1943 5238 5267 1870
Size: 73 3295 29 4545
Fragments arranged by size:
4545 3295 73 29
AvrII C'CTAG_G
Cuts at: 3067 3067
Size: 7942
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3483 3516 3483
Size: 33 7909
BamHI G'GATC_C
Cuts at: 4882 5272 5639 4882
Size: 390 367 7185
Fragments arranged by size:
7185 390 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2192 4030 5088 5263 6718
Size: 325 217 627 405 1838 1058 175 1455
Cuts at: 6718 618
Size: 1842
Fragments arranged by size:
1842 1838 1455 1058 627 405 325 217
175
BanII G_rGCy'C
Cuts at: 1391 2520 4439 4774 5261 1391
Size: 1129 1919 335 487 4072
Fragments arranged by size:
4072 1919 1129 487 335
BbeI G_GCGC'C
Cuts at: 2196 2196
Size: 7942
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2210 928
Size: 971 311 6660
Fragments arranged by size:
6660 971 311
BbvCI CC'TCA_GC
Cuts at: 4179 5060 5194 4179
Size: 881 134 6927
Fragments arranged by size:
6927 881 134
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6133 6167 1903
Size: 34 4196 34 3678
Fragments arranged by size:
4196 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5834 7044 5834
Size: 1210 6732
BclI T'GATC_A
Cuts at: 1548 4279 1548
Size: 2731 5211
BfrBI ATG'CAT
Cuts at: 2429 2752 2429
Size: 323 7619
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6553 136
Size: 107 122 71 631 605 4881 1525
Fragments arranged by size:
4881 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4472 4485 4783 4925 7937 4472
Size: 13 298 142 3012 4477
Fragments arranged by size:
4477 3012 298 142 13
BlpI GC'TnA_GC
Cuts at: 5359 5359
Size: 7942
Bme1580I G_kGCm'C
Cuts at: 1790 6003 7159 1790
Size: 4213 1156 2573
Fragments arranged by size:
4213 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7942
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3844 3849 4512
Size: 416 53 83 399 24 2427 5 663
Cuts at: 4512 4888 5673 6631 442
Size: 376 785 958 1753
Fragments arranged by size:
2427 1753 958 785 663 416 399 376
83 53 24 5
BmtI G_CTAG'C
Cuts at: 1056 4467 1056
Size: 3411 4531
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3540 4074 4788 6602 1239
Size: 2301 534 714 1814 2579
Fragments arranged by size:
2579 2301 1814 714 534
Bpu10I CC'TnA_GC
Cuts at: 3399 3771 4179 5060 5194 3399
Size: 372 408 881 134 6147
Fragments arranged by size:
6147 881 408 372 134
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3084 6067 6932 7230 3084
Size: 2983 865 298 3796
Fragments arranged by size:
3796 2983 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6605 882
Size: 617 5106 2219
Fragments arranged by size:
5106 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4757 5733 493
Size: 711 3553 976 2702
Fragments arranged by size:
3553 2702 976 711
BsaBI GATnn'nnATC
Cuts at: 7936 7936
Size: 7942
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2193 5749 6131 6766
Size: 53 83 186 936 660 3556 382 635
Cuts at: 6766 275
Size: 1451
Fragments arranged by size:
3556 1451 936 660 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6375 6773 7047 7194 7605 6375
Size: 398 274 147 411 6712
Fragments arranged by size:
6712 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4315 4345 1178
Size: 30 165 30 2912 30 4775
Fragments arranged by size:
4775 2912 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 1186
Size: 931 7011
BseYI C'CCAG_C
Cuts at: 2477 4258 4420 5210 7145 7641 2477
Size: 1781 162 790 1935 496 2778
Fragments arranged by size:
2778 1935 1781 790 496 162
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2694 2967 1163
Size: 573 958 273 6138
Fragments arranged by size:
6138 958 573 273
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5392 6153 7181 1540
Size: 275 186 3391 761 1028 2301
Fragments arranged by size:
3391 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3490 4439 4774 6003 6088 7159 3490
Size: 949 335 1229 85 1071 4273
Fragments arranged by size:
4273 1229 1071 949 335 85
BsmI GAATG_Cn'
Cuts at: 2386 5001 5453 5546 2386
Size: 2615 452 93 4782
Fragments arranged by size:
4782 2615 452 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2165 3514 2165
Size: 1349 6593
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2520 3490 4439 4774 5261 6003 6088
Size: 399 730 970 949 335 487 742 85
Cuts at: 6088 7159 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 970 949 742 730 487 399
335 85
BspEI T'CCGG_A
Cuts at: 7605 7605
Size: 7942
BspHI T'CATG_A
Cuts at: 2182 5831 7561 2182
Size: 3649 1730 2563
Fragments arranged by size:
3649 2563 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3687 4461 5100 5297 844
Size: 2843 774 639 197 3489
Fragments arranged by size:
3489 2843 774 639 197
BsrBI CCG'CTC
Cuts at: 1394 1823 3770 5829 1394
Size: 429 1947 2059 3507
Fragments arranged by size:
3507 2059 1947 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4222 5006 6437 6611 65
Size: 1183 2974 784 1431 174 1396
Fragments arranged by size:
2974 1431 1396 1183 784 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6586 6773 1700
Size: 110 4776 187 2869
Fragments arranged by size:
4776 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3028 4724 96
Size: 2932 1696 3314
Fragments arranged by size:
3314 2932 1696
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7942
BssSI C'ACGA_G
Cuts at: 6002 7014 6002
Size: 1012 6930
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2336 794
Size: 1542 6400
BstBI TT'CG_AA
Cuts at: 6746 6746
Size: 7942
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7942
BstXI CCAn_nnnn'nTGG
Cuts at: 4810 5210 4810
Size: 400 7542
BstYI r'GATC_y
Cuts at: 1972 2764 2833 4022 4472 4485 4783 4882 4925
Size: 792 69 1189 450 13 298 99 43
Cuts at: 4925 5272 5639 6024 6041 7493 7579 7591 7937
Size: 347 367 385 17 1452 86 12 346
Cuts at: 7937 1972
Size: 1977
Fragments arranged by size:
1977 1452 1189 792 450 385 367 347
346 298 99 86 69 43 17 13
12
Bsu36I CC'TnA_GG
Cuts at: 3967 5176 3967
Size: 1209 6733
BtgI C'CryG_G
Cuts at: 513 1065 3040 3743 4141 513
Size: 552 1975 703 398 4314
Fragments arranged by size:
4314 1975 703 552 398
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7203
Fragments arranged by size:
7203 683 56
BtsI GCAGTG_nn'
Cuts at: 2025 3446 5018 5458 6252 6272 2025
Size: 1421 1572 440 794 20 3695
Fragments arranged by size:
3695 1572 1421 794 440 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7942
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7907
DraI TTT'AAA
Cuts at: 2556 3318 5246 2556
Size: 762 1928 5252
Fragments arranged by size:
5252 1928 762
DraIII CAC_nnn'GTG
Cuts at: 3485 3485
Size: 7942
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6949 809
Size: 507 5633 1802
Fragments arranged by size:
5633 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2845 3214 5149
Size: 54 1425 50 275 18 1015 369 1935
Cuts at: 5149 5389 6278 7907 8
Size: 240 889 1629 43
Fragments arranged by size:
1935 1629 1425 1015 889 369 275 240
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5389 1537
Size: 275 3577 4090
Fragments arranged by size:
4090 3577 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2094 2168 3732 4300 4309 5872 1353
Size: 741 74 1564 568 9 1563 3423
Fragments arranged by size:
3423 1564 1563 741 568 74 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2087 5019 6529 6903 7049 387
Size: 1064 138 498 2932 1510 374 146 1280
Fragments arranged by size:
2932 1510 1280 1064 498 374 146 138
EcoICRI GAG'CTC
Cuts at: 4437 4772 4437
Size: 335 7607
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2277 3540 4074 4290 4788 6005 6602
Size: 393 645 1263 534 216 498 1217 597
Cuts at: 6602 7389 7657 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1263 1217 787 645 597 534 498
393 268 216
EcoO109I rG'GnC_Cy
Cuts at: 3809 3809
Size: 7942
EcoRI G'AATT_C
Cuts at: 3417 5251 5629 3417
Size: 1834 378 5730
Fragments arranged by size:
5730 1834 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2480 2480
Size: 7942
FspI TGC'GCA
Cuts at: 2017 6448 2017
Size: 4431 3511
HaeII r_GCGC'y
Cuts at: 1678 2141 2196 7089 1678
Size: 463 55 4893 2531
Fragments arranged by size:
4893 2531 463 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2502 2531 2534 2563 2641
Size: 32 135 32 930 29 3 29 78
Cuts at: 2641 2673 5000 5032 6583 6615 6657 6689 1373
Size: 32 2327 32 1551 32 42 32 2626
Fragments arranged by size:
2626 2327 1551 930 135 78 42 32
32 32 32 32 32 29 29 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2473 5286 5532 669
Size: 988 281 535 2813 246 3079
Fragments arranged by size:
3079 2813 988 535 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7942
HpaI GTT'AAC
Cuts at: 5532 5532
Size: 7942
KasI G'GCGC_C
Cuts at: 2192 2192
Size: 7942
KpnI G_GTAC'C
Cuts at: 5267 5267
Size: 7942
MfeI C'AATT_G
Cuts at: 3032 4938 5541 3032
Size: 1906 603 5433
Fragments arranged by size:
5433 1906 603
MluI A'CGCG_T
Cuts at: 7818 7818
Size: 7942
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2292 2726 7056 7240 1305
Size: 987 434 4330 184 2007
Fragments arranged by size:
4330 2007 987 434 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2847 3216 5151 10
Size: 54 1425 343 1015 369 1935 2801
Fragments arranged by size:
2801 1935 1425 1015 369 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2187 2637 3045 5900 6259 6418 518
Size: 648 1021 450 408 2855 359 159 2042
Fragments arranged by size:
2855 2042 1021 648 450 408 359 159
MspA1I CmG'CkG
Cuts at: 1606 2039 3340 4262 5358 6035 7183 7428 1606
Size: 433 1301 922 1096 677 1148 245 2120
Fragments arranged by size:
2120 1301 1148 1096 922 677 433 245
NarI GG'CG_CC
Cuts at: 2193 2193
Size: 7942
NcoI C'CATG_G
Cuts at: 513 1065 3040 3743 4141 513
Size: 552 1975 703 398 4314
Fragments arranged by size:
4314 1975 703 552 398
NdeI CA'TA_TG
Cuts at: 387 2677 4833 387
Size: 2290 2156 3496
Fragments arranged by size:
3496 2290 2156
NheI G'CTAG_C
Cuts at: 1052 4463 1052
Size: 3411 4531
NotI GC'GGCC_GC
Cuts at: 5389 5389
Size: 7942
NsiI A_TGCA'T
Cuts at: 2431 2754 2431
Size: 323 7619
NspI r_CATG'y
Cuts at: 1132 1229 3050 4545 5300 6845 1132
Size: 97 1821 1495 755 1545 2229
Fragments arranged by size:
2229 1821 1545 1495 755 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7942
PciI A'CATG_T
Cuts at: 6841 6841
Size: 7942
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7942
PfoI T'CCnGG_A
Cuts at: 4974 4974
Size: 7942
PmeI GTTT'AAAC
Cuts at: 5246 5246
Size: 7942
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6011 6043 7524 7556 6011
Size: 32 1481 32 6397
Fragments arranged by size:
6397 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3809 3809
Size: 7942
PshAI GACnn'nnGTC
Cuts at: 1613 2157 1613
Size: 544 7398
PsiI TTA'TAA
Cuts at: 5512 5512
Size: 7942
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2525 2557 2525
Size: 32 7910
PstI C_TGCA'G
Cuts at: 830 3574 3684 5025 5294 830
Size: 2744 110 1341 269 3478
Fragments arranged by size:
3478 2744 1341 269 110
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7942
PvuII CAG'CTG
Cuts at: 3340 4262 3340
Size: 922 7020
SacI G_AGCT'C
Cuts at: 4439 4774 4439
Size: 335 7607
SalI G'TCGA_C
Cuts at: 1936 5284 1936
Size: 3348 4594
SapI GCTCTTCn'nnn_
Cuts at: 3732 3732
Size: 7942
SbfI CC_TGCA'GG
Cuts at: 5294 5294
Size: 7942
ScaI AGT'ACT
Cuts at: 6190 7902 6190
Size: 1712 6230
SfcI C'TryA_G
Cuts at: 826 1045 3185 3570 3680 5021 5290 6425 7106
Size: 219 2140 385 110 1341 269 1135 681
Cuts at: 7106 7297 826
Size: 191 1471
Fragments arranged by size:
2140 1471 1341 1135 681 385 269 219
191 110
SfoI GGC'GCC
Cuts at: 2194 2194
Size: 7942
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7942
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7942
SmaI CCC'GGG
Cuts at: 1872 5240 5269 1872
Size: 3368 29 4545
Fragments arranged by size:
4545 3368 29
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3099 6046 6947 7209 820
Size: 197 926 1156 2947 901 262 1553
Fragments arranged by size:
2947 1553 1156 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5733 493
Size: 5240 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7942
SphI G_CATG'C
Cuts at: 1132 3050 4545 5300 1132
Size: 1918 1495 755 3774
Fragments arranged by size:
3774 1918 1495 755
SspI AAT'ATT
Cuts at: 5 52 4252 5866 5
Size: 47 4200 1614 2081
Fragments arranged by size:
4200 2081 1614 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7942
StyI C'CwwG_G
Cuts at: 513 1065 2401 2493 3040 3067 3743 4026 4141
Size: 552 1336 92 547 27 676 283 115
Cuts at: 4141 5381 513
Size: 1240 3074
Fragments arranged by size:
3074 1336 1240 676 552 547 283 115
92 27
SwaI ATTT'AAAT
Cuts at: 3318 3318
Size: 7942
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4272 4675 5969 5986 6139 6324 6769
Size: 1255 2692 403 1294 17 153 185 445
Cuts at: 6769 325
Size: 1498
Fragments arranged by size:
2692 1498 1294 1255 445 403 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2594 3028 3238
Size: 274 80 33 51 1443 617 434 210
Cuts at: 3238 3611 4724 6188 7900 96
Size: 373 1113 1464 1712 138
Fragments arranged by size:
1712 1464 1443 1113 617 434 373 274
210 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5206 5238 7902 7934 5206
Size: 32 2664 32 5214
Fragments arranged by size:
5214 2664 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7942
XbaI T'CTAG_A
Cuts at: 5278 5278
Size: 7942
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4216 4216
Size: 7942
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7942
XmaI C'CCGG_G
Cuts at: 1870 5238 5267 1870
Size: 3368 29 4545
Fragments arranged by size:
4545 3368 29
XmnI GAAnn'nnTTC
Cuts at: 2703 6071 2703
Size: 3368 4574
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5750 276
Size: 53 83 186 936 4216 2468
Fragments arranged by size:
4216 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BplI BsiWI BstZ17I EcoNI
EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI
PspOMI PspXI RsrII SacII SanDI SexAI SfiI SrfI