Restriction map for
pFN21AB7312
(Circular) (Six-base) MAPSORT of: pFN21AB7312.seq Check: 4775 from: 1 to: 7882
pFN21AB7312 7882 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4516 4516
Size: 7882
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4620 5692 278
Size: 53 83 186 936 3084 1072 2468
Fragments arranged by size:
3084 2468 1072 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4044 4620 5225 1937
Size: 2107 576 605 4594
Fragments arranged by size:
4594 2107 605 576
Acc65I G'GTAC_C
Cuts at: 5203 5203
Size: 7882
AclI AA'CG_TT
Cuts at: 1563 6009 6382 1563
Size: 4446 373 3063
Fragments arranged by size:
4446 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3587 5945 7329 7597 1632
Size: 1955 2358 1384 268 1917
Fragments arranged by size:
2358 1955 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7685
AflIII A'CryG_T
Cuts at: 1203 6781 7758 1203
Size: 5578 977 1327
Fragments arranged by size:
5578 1327 977
AgeI A'CCGG_T
Cuts at: 6713 6713
Size: 7882
AhdI GACnn_n'nnGTC
Cuts at: 3176 6611 3176
Size: 3435 4447
AleI CACnn'nnGTG
Cuts at: 1166 2396 2685 3653 1166
Size: 1230 289 968 5395
Fragments arranged by size:
5395 1230 968 289
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4994 1184
Size: 3810 4072
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2465 2497 2465
Size: 32 7850
AlwNI CAG_nnn'CTG
Cuts at: 2305 3073 5625 7197 2305
Size: 768 2552 1572 2990
Fragments arranged by size:
2990 2552 1572 768
ApaI G_GGCC'C
Cuts at: 2824 3673 4073 2824
Size: 849 400 6633
Fragments arranged by size:
6633 849 400
ApaLI G'TGCA_C
Cuts at: 5939 7095 5939
Size: 1156 6726
ApoI r'AATT_y
Cuts at: 1493 5191 5417 5569 7678 1493
Size: 3698 226 152 2109 1697
Fragments arranged by size:
3698 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6436 160
Size: 872 5404 1606
Fragments arranged by size:
5404 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7882
AvaI C'yCGr_G
Cuts at: 1870 1943 2635 3270 3510 3929 4297 4814 5020
Size: 73 692 635 240 419 368 517 206
Cuts at: 5020 5136 5207 1870
Size: 116 71 4545
Fragments arranged by size:
4545 692 635 517 419 368 240 206
116 73 71
AvrII C'CTAG_G
Cuts at: 4651 4651
Size: 7882
BamHI G'GATC_C
Cuts at: 2343 3294 4194 5212 5579 2343
Size: 951 900 1018 367 4646
Fragments arranged by size:
4646 1018 951 900 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3547 4462 4545 5203 6658
Size: 325 217 627 1760 915 83 658 1455
Cuts at: 6658 618
Size: 1842
Fragments arranged by size:
1842 1760 1455 915 658 627 325 217
83
BanII G_rGCy'C
Cuts at: 1391 2013 2412 2824 3192 3395 3673 3713 3936
Size: 622 399 412 368 203 278 40 223
Cuts at: 3936 4073 4821 4871 5058 5201 1391
Size: 137 748 50 187 143 4072
Fragments arranged by size:
4072 748 622 412 399 368 278 223
203 187 143 137 50 40
BbeI G_GCGC'C
Cuts at: 4466 4549 4466
Size: 83 7799
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6911
BbvCI CC'TCA_GC
Cuts at: 2792 4928 2792
Size: 2136 5746
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6073 6107 1903
Size: 34 4136 34 3678
Fragments arranged by size:
4136 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5774 6984 5774
Size: 1210 6672
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7882
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4424 6493 136
Size: 107 122 71 631 605 2752 2069 1525
Fragments arranged by size:
2752 2069 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7877 7877
Size: 7882
BlpI GC'TnA_GC
Cuts at: 2306 2406 5299 2306
Size: 100 2893 4889
Fragments arranged by size:
4889 2893 100
Bme1580I G_kGCm'C
Cuts at: 1790 2040 2824 3552 3673 3683 4073 5943 7099
Size: 250 784 728 121 10 390 1870 1156
Cuts at: 7099 1790
Size: 2573
Fragments arranged by size:
2573 1870 1156 784 728 390 250 121
10
BmgBI CAC'GTC
Cuts at: 1318 2881 4750 1318
Size: 1563 1869 4450
Fragments arranged by size:
4450 1869 1563
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3460 4952 5088
Size: 416 53 83 399 24 2043 1492 136
Cuts at: 5088 5613 6571 442
Size: 525 958 1753
Fragments arranged by size:
2043 1753 1492 958 525 416 399 136
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7882
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2560 3208 2560
Size: 648 7234
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2282 2285 2339 2614 2912 3320 3812 4220
Size: 1043 3 54 275 298 408 492 408
Cuts at: 4220 4709 4781 6542 1239
Size: 489 72 1761 2579
Fragments arranged by size:
2579 1761 1043 492 489 408 408 298
275 72 54 3
Bpu10I CC'TnA_GC
Cuts at: 2792 3465 4143 4667 4928 2792
Size: 673 678 524 261 5746
Fragments arranged by size:
5746 678 673 524 261
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2990 6007 6872 7170 2990
Size: 3017 865 298 3702
Fragments arranged by size:
3702 3017 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2222 6545 882
Size: 617 723 4323 2219
Fragments arranged by size:
4323 2219 723 617
BsaAI yAC'GTr
Cuts at: 493 1204 2173 2776 3287 5673 493
Size: 711 969 603 511 2386 2702
Fragments arranged by size:
2702 2386 969 711 603 511
BsaBI GATnn'nnATC
Cuts at: 7876 7876
Size: 7882
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2255 3253 3947 4403
Size: 53 83 186 936 722 998 694 456
Cuts at: 4403 4416 4463 4546 4617 5689 6071 6706 275
Size: 13 47 83 71 1072 382 635 1451
Fragments arranged by size:
1451 1072 998 936 722 694 635 456
382 186 83 83 71 53 47 13
BsaWI w'CCGG_w
Cuts at: 2202 3890 6315 6713 6987 7134 7545 2202
Size: 1688 2425 398 274 147 411 2539
Fragments arranged by size:
2539 2425 1688 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2357 2387 2633 2663 3328
Size: 30 165 30 954 30 246 30 665
Cuts at: 3328 3358 3585 3615 3980 4010 4193 4223 4505
Size: 30 227 30 365 30 183 30 282
Cuts at: 4505 4535 1178
Size: 30 4525
Fragments arranged by size:
4525 954 665 365 282 246 227 183
165 30 30 30 30 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2336 2339 3200 4100 4916 1186
Size: 1150 3 861 900 816 4152
Fragments arranged by size:
4152 1150 900 861 816 3
BseYI C'CCAG_C
Cuts at: 2969 2994 3225 3362 4125 4215 4310 4440 4863
Size: 25 231 137 763 90 95 130 423
Cuts at: 4863 5030 5091 7085 7581 2969
Size: 167 61 1994 496 3270
Fragments arranged by size:
3270 1994 763 496 423 231 167 137
130 95 90 61 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2261 3728 4982 1163
Size: 573 525 1467 1254 4063
Fragments arranged by size:
4063 1467 1254 573 525
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2187 2871 2920 4470 4917 5332
Size: 275 186 186 684 49 1550 447 415
Cuts at: 5332 6093 7121 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1550 1028 761 684 447 415 275
186 186 49
BsiHKAI G_wGCw'C
Cuts at: 2367 2412 2682 3936 3987 4385 5943 6028 7099
Size: 45 270 1254 51 398 1558 85 1071
Cuts at: 7099 2367
Size: 3150
Fragments arranged by size:
3150 1558 1254 1071 398 270 85 51
45
BsmI GAATG_Cn'
Cuts at: 5393 5486 5393
Size: 93 7789
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2040 2367 2412 2682 2824 3192
Size: 399 223 27 327 45 270 142 368
Cuts at: 3192 3395 3552 3673 3683 3713 3936 3987 4073
Size: 203 157 121 10 30 223 51 86
Cuts at: 4073 4385 4821 4871 5058 5201 5943 6028 7099
Size: 312 436 50 187 143 742 85 1071
Cuts at: 7099 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 436 399 368 327 312
270 223 223 203 187 157 143 142
121 86 85 51 50 45 30 27
10
BspEI T'CCGG_A
Cuts at: 3890 7545 3890
Size: 3655 4227
BspHI T'CATG_A
Cuts at: 5771 7501 5771
Size: 1730 6152
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3547 3652 4363 4516 4949 5237 844
Size: 2703 105 711 153 433 288 3489
Fragments arranged by size:
3489 2703 711 433 288 153 105
BsrBI CCG'CTC
Cuts at: 1394 1823 2063 2087 4671 5769 1394
Size: 429 240 24 2584 1098 3507
Fragments arranged by size:
3507 2584 1098 429 240 24
BsrDI GCAATG_nn'
Cuts at: 65 1248 3501 6377 6551 65
Size: 1183 2253 2876 174 1396
Fragments arranged by size:
2876 2253 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2603 2919 3198 4465 4774 4859 4988
Size: 110 793 316 279 1267 309 85 129
Cuts at: 4988 5109 6526 6713 1700
Size: 121 1417 187 2869
Fragments arranged by size:
2869 1417 1267 793 316 309 279 187
129 121 110 85
BsrGI T'GTAC_A
Cuts at: 96 2853 4164 96
Size: 2757 1311 3814
Fragments arranged by size:
3814 2757 1311
BssHII G'CGCG_C
Cuts at: 1925 4700 1925
Size: 2775 5107
BssSI C'ACGA_G
Cuts at: 2033 5942 6954 2033
Size: 3909 1012 2961
Fragments arranged by size:
3909 2961 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2284 2979 3077 4017 4848 794
Size: 1490 695 98 940 831 3828
Fragments arranged by size:
3828 1490 940 831 695 98
BstBI TT'CG_AA
Cuts at: 6686 6686
Size: 7882
BstEII G'GTnAC_C
Cuts at: 1184 3454 1184
Size: 2270 5612
BstXI CCAn_nnnn'nTGG
Cuts at: 2716 3808 4486 2716
Size: 1092 678 6112
Fragments arranged by size:
6112 1092 678
BstYI r'GATC_y
Cuts at: 1972 2343 3294 4194 5212 5579 5964 5981 7433
Size: 371 951 900 1018 367 385 17 1452
Cuts at: 7433 7519 7531 7877 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1018 951 900 385 371 367
346 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2648 3339 3951 4507 4520 4890
Size: 552 939 644 691 612 556 13 370
Cuts at: 4890 513
Size: 3505
Fragments arranged by size:
3505 939 691 644 612 556 552 370
13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7143
Fragments arranged by size:
7143 683 56
BtsI GCAGTG_nn'
Cuts at: 2270 2566 2731 3634 3681 4386 4606 4643 5398
Size: 296 165 903 47 705 220 37 755
Cuts at: 5398 6192 6212 2270
Size: 794 20 3940
Fragments arranged by size:
3940 903 794 755 705 296 220 165
47 37 20
ClaI AT'CG_AT
Cuts at: 1554 3419 1554
Size: 1865 6017
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2098 2133 3585 3620 529
Size: 35 1534 35 1452 35 4791
Fragments arranged by size:
4791 1534 1452 35 35 35
DraI TTT'AAA
Cuts at: 5186 5186
Size: 7882
DraIII CAC_nnn'GTG
Cuts at: 2035 3077 2035
Size: 1042 6840
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3020 3785 6889 809
Size: 507 1704 765 3104 1802
Fragments arranged by size:
3104 1802 1704 765 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2088 2106 2184
Size: 54 1425 50 275 18 258 18 78
Cuts at: 2184 2601 2868 2917 3091 3196 3798 3849 4257
Size: 417 267 49 174 105 602 51 408
Cuts at: 4257 4467 4517 4914 5107 5329 6218 7847 8
Size: 210 50 397 193 222 889 1629 43
Fragments arranged by size:
1629 1425 889 602 417 408 397 275
267 258 222 210 193 174 105 78
54 51 50 50 49 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2184 2868 2917 4467 4914 5329 1537
Size: 275 372 684 49 1550 447 415 4090
Fragments arranged by size:
4090 1550 684 447 415 372 275 49
EarI CTCTTCn'nnn_
Cuts at: 1353 5812 1353
Size: 4459 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6469 6843 6989 387
Size: 1064 138 4880 374 146 1280
Fragments arranged by size:
4880 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2410 3934 2410
Size: 1524 6358
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2282 2285 2339 2614 2912 3320 3587
Size: 393 650 3 54 275 298 408 267
Cuts at: 3587 3812 4220 4709 4781 5945 6542 7329 7597
Size: 225 408 489 72 1164 597 787 268
Cuts at: 7597 1239
Size: 1524
Fragments arranged by size:
1524 1164 787 650 597 489 408 408
393 298 275 268 267 225 72 54
3
EcoNI CCTnn'n_nnAGG
Cuts at: 2321 3008 3941 2321
Size: 687 933 6262
Fragments arranged by size:
6262 933 687
EcoO109I rG'GnC_Cy
Cuts at: 2429 3170 3427 3576 4069 4070 4090 4627 5036
Size: 741 257 149 493 1 20 537 409
Cuts at: 5036 2429
Size: 5275
Fragments arranged by size:
5275 741 537 493 409 257 149 20
1
EcoRI G'AATT_C
Cuts at: 5191 5569 5191
Size: 378 7504
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2449 2678 2698 3031 2449
Size: 229 20 333 7300
Fragments arranged by size:
7300 333 229 20
FseI GG_CCGG'CC
Cuts at: 5113 5113
Size: 7882
FspI TGC'GCA
Cuts at: 4021 6388 4021
Size: 2367 5515
HaeII r_GCGC'y
Cuts at: 1678 4466 4549 4757 7029 1678
Size: 2788 83 208 2272 2531
Fragments arranged by size:
2788 2531 2272 208 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2438 2470 2890 2922 3162
Size: 32 135 32 866 32 420 32 240
Cuts at: 3162 3194 4193 4225 5003 5035 5157 5189 6523
Size: 32 999 32 778 32 122 32 1334
Cuts at: 6523 6555 6597 6629 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1334 999 866 778 420 240 135
122 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4045 5226 5472 669
Size: 988 281 2107 1181 246 3079
Fragments arranged by size:
3079 2107 1181 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7882
HpaI GTT'AAC
Cuts at: 5472 5472
Size: 7882
KasI G'GCGC_C
Cuts at: 4462 4545 4462
Size: 83 7799
KpnI G_GTAC'C
Cuts at: 5207 5207
Size: 7882
MfeI C'AATT_G
Cuts at: 2394 5481 2394
Size: 3087 4795
MluI A'CGCG_T
Cuts at: 7758 7758
Size: 7882
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2580 6996 7180 1305
Size: 1275 4416 184 2007
Fragments arranged by size:
4416 2007 1275 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2108 3800 3851 4259 10
Size: 54 1425 343 276 1692 51 408 3633
Fragments arranged by size:
3633 1692 1425 408 343 276 54 51
MslI CAynn'nnrTG
Cuts at: 518 1166 2099 2396 2685 3653 4250 5840 6199
Size: 648 933 297 289 968 597 1590 359
Cuts at: 6199 6358 518
Size: 159 2042
Fragments arranged by size:
2042 1590 968 933 648 597 359 297
289 159
MspA1I CmG'CkG
Cuts at: 1606 2463 2796 3615 4283 4502 4687 4903 5016
Size: 857 333 819 668 219 185 216 113
Cuts at: 5016 5147 5298 5975 7123 7368 1606
Size: 131 151 677 1148 245 2120
Fragments arranged by size:
2120 1148 857 819 677 668 333 245
219 216 185 151 131 113
NaeI GCC'GGC
Cuts at: 2605 2921 4467 4990 5111 2605
Size: 316 1546 523 121 5376
Fragments arranged by size:
5376 1546 523 316 121
NarI GG'CG_CC
Cuts at: 4463 4546 4463
Size: 83 7799
NcoI C'CATG_G
Cuts at: 513 1065 2004 3339 4890 513
Size: 552 939 1335 1551 3505
Fragments arranged by size:
3505 1551 1335 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7882
NgoMIV G'CCGG_C
Cuts at: 2603 2919 4465 4988 5109 2603
Size: 316 1546 523 121 5376
Fragments arranged by size:
5376 1546 523 316 121
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7882
NotI GC'GGCC_GC
Cuts at: 5329 5329
Size: 7882
NspI r_CATG'y
Cuts at: 1132 1229 3325 4981 5240 6785 1132
Size: 97 2096 1656 259 1545 2229
Fragments arranged by size:
2229 2096 1656 1545 259 97
PasI CC'CwG_GG
Cuts at: 1348 2131 4325 1348
Size: 783 2194 4905
Fragments arranged by size:
4905 2194 783
PciI A'CATG_T
Cuts at: 6781 6781
Size: 7882
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4454 4638 1108
Size: 3346 184 4352
Fragments arranged by size:
4352 3346 184
PfoI T'CCnGG_A
Cuts at: 3297 3768 3789 4029 4197 5175 3297
Size: 471 21 240 168 978 6004
Fragments arranged by size:
6004 978 471 240 168 21
PmeI GTTT'AAAC
Cuts at: 5186 5186
Size: 7882
PmlI CAC'GTG
Cuts at: 2173 2173
Size: 7882
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5951 5983 7464 7496 5951
Size: 32 1481 32 6337
Fragments arranged by size:
6337 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2429 3170 3576 4627 5036 2429
Size: 741 406 1051 409 5275
Fragments arranged by size:
5275 1051 741 409 406
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7882
PsiI TTA'TAA
Cuts at: 5452 5452
Size: 7882
PspOMI G'GGCC_C
Cuts at: 2820 3669 4069 2820
Size: 849 400 6633
Fragments arranged by size:
6633 849 400
PstI C_TGCA'G
Cuts at: 830 3042 3942 4908 5051 5234 830
Size: 2212 900 966 143 183 3478
Fragments arranged by size:
3478 2212 966 900 183 143
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7882
PvuII CAG'CTG
Cuts at: 4283 4687 4903 5016 5147 4283
Size: 404 216 113 131 7018
Fragments arranged by size:
7018 404 216 131 113
SacI G_AGCT'C
Cuts at: 2412 3936 2412
Size: 1524 6358
SalI G'TCGA_C
Cuts at: 1936 4043 5224 1936
Size: 2107 1181 4594
Fragments arranged by size:
4594 2107 1181
SanDI GG'GwC_CC
Cuts at: 2429 3576 4627 5036 2429
Size: 1147 1051 409 5275
Fragments arranged by size:
5275 1147 1051 409
SbfI CC_TGCA'GG
Cuts at: 5051 5234 5051
Size: 183 7699
ScaI AGT'ACT
Cuts at: 6130 7842 6130
Size: 1712 6170
SfcI C'TryA_G
Cuts at: 826 1045 3038 3938 3976 4904 5047 5230 6365
Size: 219 1993 900 38 928 143 183 1135
Cuts at: 6365 7046 7237 826
Size: 681 191 1471
Fragments arranged by size:
1993 1471 1135 928 900 681 219 191
183 143 38
SfoI GGC'GCC
Cuts at: 4464 4547 4464
Size: 83 7799
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7882
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7882
SmaI CCC'GGG
Cuts at: 1872 2637 3512 4299 5138 5209 1872
Size: 765 875 787 839 71 4545
Fragments arranged by size:
4545 875 839 787 765 71
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3005 5986 6887 7149 820
Size: 197 926 1062 2981 901 262 1553
Fragments arranged by size:
2981 1553 1062 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5673 493
Size: 5180 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7882
SphI G_CATG'C
Cuts at: 1132 3325 4981 5240 1132
Size: 2193 1656 259 3774
Fragments arranged by size:
3774 2193 1656 259
SspI AAT'ATT
Cuts at: 5 52 5806 5
Size: 47 5754 2081
Fragments arranged by size:
5754 2081 47
StuI AGG'CCT
Cuts at: 1517 3490 1517
Size: 1973 5909
StyI C'CwwG_G
Cuts at: 513 1065 2004 2049 2433 2508 3339 4651 4890
Size: 552 939 45 384 75 831 1312 239
Cuts at: 4890 5321 513
Size: 431 3074
Fragments arranged by size:
3074 1312 939 831 552 431 384 239
75 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2755 2768 2983 4324 4454 5059 5909
Size: 1255 1175 13 215 1341 130 605 850
Cuts at: 5909 5926 6079 6264 6709 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1341 1255 1175 850 605 445 215
185 153 130 17 13
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2853 4164 6128
Size: 274 80 33 51 1443 876 1311 1964
Cuts at: 6128 7840 96
Size: 1712 138
Fragments arranged by size:
1964 1712 1443 1311 876 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2060 2092 2151 2183 2357 2389 2633 2665 2946
Size: 32 59 32 174 32 244 32 281
Cuts at: 2946 2978 3328 3360 3585 3617 4505 4537 5055
Size: 32 350 32 225 32 888 32 518
Cuts at: 5055 5087 7842 7874 2060
Size: 32 2755 32 2068
Fragments arranged by size:
2755 2068 888 518 350 281 244 225
174 59 32 32 32 32 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3452 1316
Size: 2136 5746
XbaI T'CTAG_A
Cuts at: 5218 5218
Size: 7882
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2098 3325 3493 3514 4061 4252 2098
Size: 1227 168 21 547 191 5728
Fragments arranged by size:
5728 1227 547 191 168 21
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7882
XmaI C'CCGG_G
Cuts at: 1870 2635 3510 4297 5136 5207 1870
Size: 765 875 787 839 71 4545
Fragments arranged by size:
4545 875 839 787 765 71
XmnI GAAnn'nnTTC
Cuts at: 6011 6011
Size: 7882
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4618 5690 276
Size: 53 83 186 936 3084 1072 2468
Fragments arranged by size:
3084 2468 1072 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I
EcoRV FspAI NruI NsiI PacI PspXI PsrI RsrII
SacII SapI SexAI SfiI SrfI SwaI