Restriction map for
pFN21AB7309
(Circular) (Six-base) MAPSORT of: pFN21AB7309.seq Check: 1518 from: 1 to: 7438
pFN21AB7309 7438 bp
With 182 enzymes: SgfI *
December 8, 2014 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4471 4471
Size: 7438
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5248 278
Size: 53 83 186 936 3712 2468
Fragments arranged by size:
3712 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2051 4781 1937
Size: 114 2730 4594
Fragments arranged by size:
4594 2730 114
Acc65I G'GTAC_C
Cuts at: 4759 4759
Size: 7438
AclI AA'CG_TT
Cuts at: 1563 5565 5938 1563
Size: 4002 373 3063
Fragments arranged by size:
4002 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5501 6885 7153 1632
Size: 3869 1384 268 1917
Fragments arranged by size:
3869 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7241
AflIII A'CryG_T
Cuts at: 1203 2707 3582 4721 6337 7314 1203
Size: 1504 875 1139 1616 977 1327
Fragments arranged by size:
1616 1504 1327 1139 977 875
AgeI A'CCGG_T
Cuts at: 6269 6269
Size: 7438
AhdI GACnn_n'nnGTC
Cuts at: 6167 6167
Size: 7438
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7438
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3627 1184
Size: 2443 4995
AlwNI CAG_nnn'CTG
Cuts at: 2075 2576 2974 3607 3622 4381 5181 6753 2075
Size: 501 398 633 15 759 800 1572 2760
Fragments arranged by size:
2760 1572 800 759 633 501 398 15
ApaI G_GGCC'C
Cuts at: 3950 3950
Size: 7438
ApaLI G'TGCA_C
Cuts at: 2349 4179 5495 6651 2349
Size: 1830 1316 1156 3136
Fragments arranged by size:
3136 1830 1316 1156
ApoI r'AATT_y
Cuts at: 1493 2305 2672 4683 4747 4973 5125 7234 1493
Size: 812 367 2011 64 226 152 2109 1697
Fragments arranged by size:
2109 2011 1697 812 367 226 152 64
AseI AT'TA_AT
Cuts at: 160 1032 5992 160
Size: 872 4960 1606
Fragments arranged by size:
4960 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7438
AvaI C'yCGr_G
Cuts at: 1870 1943 2023 4763 1870
Size: 73 80 2740 4545
Fragments arranged by size:
4545 2740 80 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4246 4279 4246
Size: 33 7405
BamHI G'GATC_C
Cuts at: 2934 4768 5135 2934
Size: 1834 367 5237
Fragments arranged by size:
5237 1834 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4759 6214 618
Size: 325 217 627 2972 1455 1842
Fragments arranged by size:
2972 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3950 4112 4458 4562 4757 1391
Size: 2559 162 346 104 195 4072
Fragments arranged by size:
4072 2559 346 195 162 104
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2045 3579 4449 928
Size: 971 146 1534 870 3917
Fragments arranged by size:
3917 1534 971 870 146
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2947 2981 5629 5663 1903
Size: 34 1010 34 2648 34 3678
Fragments arranged by size:
3678 2648 1010 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3116 5330 6540 3116
Size: 2214 1210 4014
Fragments arranged by size:
4014 2214 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7438
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3494 3700 6049
Size: 107 122 71 631 605 1822 206 2349
Cuts at: 6049 136
Size: 1525
Fragments arranged by size:
2349 1822 1525 631 605 206 122 107
71
BglII A'GATC_T
Cuts at: 2394 2907 7433 2394
Size: 513 4526 2399
Fragments arranged by size:
4526 2399 513
BlpI GC'TnA_GC
Cuts at: 4855 4855
Size: 7438
Bme1580I G_kGCm'C
Cuts at: 1790 2353 3616 3950 4183 4316 5499 6655 1790
Size: 563 1263 334 233 133 1183 1156 2573
Fragments arranged by size:
2573 1263 1183 1156 563 334 233 133
BmgBI CAC'GTC
Cuts at: 1318 3585 1318
Size: 2267 5171
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2389 4010 4048
Size: 416 53 83 399 24 972 1621 38
Cuts at: 4048 5169 6127 442
Size: 1121 958 1753
Fragments arranged by size:
1753 1621 1121 972 958 416 399 83
53 38 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7438
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3336 3336
Size: 7438
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2052 2089 2573 3577 3644 3790 4025 4474
Size: 813 37 484 1004 67 146 235 449
Cuts at: 4474 6098 1239
Size: 1624 2579
Fragments arranged by size:
2579 1624 1004 813 484 449 235 146
67 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3344 5563 6428 6726 3344
Size: 2219 865 298 4056
Fragments arranged by size:
4056 2219 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4524 6101 882
Size: 617 3025 1577 2219
Fragments arranged by size:
3025 2219 1577 617
BsaAI yAC'GTr
Cuts at: 493 1204 2710 4724 5229 493
Size: 711 1506 2014 505 2702
Fragments arranged by size:
2702 2014 1506 711 505
BsaBI GATnn'nnATC
Cuts at: 2277 2761 7432 2277
Size: 484 4671 2283
Fragments arranged by size:
4671 2283 484
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5245 5627 6262 275
Size: 53 83 186 936 3712 382 635 1451
Fragments arranged by size:
3712 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2088 2313 5871 6269 6543 6690 7101 2088
Size: 225 3558 398 274 147 411 2425
Fragments arranged by size:
3558 2425 411 398 274 225 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2705 2735 1178
Size: 30 165 30 1302 30 5881
Fragments arranged by size:
5881 1302 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4420 4423 4487 1186
Size: 3234 3 64 4137
Fragments arranged by size:
4137 3234 64 3
BseYI C'CCAG_C
Cuts at: 3215 4196 4309 6641 7137 3215
Size: 981 113 2332 496 3516
Fragments arranged by size:
3516 2332 981 496 113
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6865
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4888 5649 6677 1540
Size: 275 186 2887 761 1028 2301
Fragments arranged by size:
2887 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2353 3803 3933 4112 4183 4458 5499 5584 6655
Size: 1450 130 179 71 275 1041 85 1071
Cuts at: 6655 2353
Size: 3136
Fragments arranged by size:
3136 1450 1071 1041 275 179 130 85
71
BsmI GAATG_Cn'
Cuts at: 2953 4949 5042 2953
Size: 1996 93 5349
Fragments arranged by size:
5349 1996 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2353 3616 3803 3933 3950 4112 4183
Size: 399 563 1263 187 130 17 162 71
Cuts at: 4183 4316 4458 4562 4757 5499 5584 6655 1391
Size: 133 142 104 195 742 85 1071 2174
Fragments arranged by size:
2174 1263 1071 742 563 399 195 187
162 142 133 130 104 85 71 17
BspEI T'CCGG_A
Cuts at: 2088 7101 2088
Size: 5013 2425
BspHI T'CATG_A
Cuts at: 5327 7057 5327
Size: 1730 5708
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2522 3991 4471 4793 844
Size: 1678 1469 480 322 3489
Fragments arranged by size:
3489 1678 1469 480 322
BsrBI CCG'CTC
Cuts at: 1394 1823 3328 5325 1394
Size: 429 1505 1997 3507
Fragments arranged by size:
3507 1997 1505 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2625 5933 6107 65
Size: 1183 1377 3308 174 1396
Fragments arranged by size:
3308 1396 1377 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3474 6082 6269 1700
Size: 110 1664 2608 187 2869
Fragments arranged by size:
2869 2608 1664 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7438
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7438
BssSI C'ACGA_G
Cuts at: 2213 3566 5498 6510 2213
Size: 1353 1932 1012 3141
Fragments arranged by size:
3141 1932 1353 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2100 794
Size: 1306 6132
BstBI TT'CG_AA
Cuts at: 6242 6242
Size: 7438
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7438
BstXI CCAn_nnnn'nTGG
Cuts at: 2778 3477 2778
Size: 699 6739
BstYI r'GATC_y
Cuts at: 1972 2394 2583 2907 2934 4142 4768 5135 5520
Size: 422 189 324 27 1208 626 367 385
Cuts at: 5520 5537 6989 7075 7087 7433 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1208 626 422 385 367 346
324 189 86 27 17 12
Bsu36I CC'TnA_GG
Cuts at: 2930 2987 2930
Size: 57 7381
BtgI C'CryG_G
Cuts at: 513 1065 2004 2739 3033 3765 513
Size: 552 939 735 294 732 4186
Fragments arranged by size:
4186 939 735 732 552 294
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6699
Fragments arranged by size:
6699 683 56
BtsI GCAGTG_nn'
Cuts at: 4183 4211 4558 4954 5748 5768 4183
Size: 28 347 396 794 20 5853
Fragments arranged by size:
5853 794 396 347 28 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7438
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7403
DraI TTT'AAA
Cuts at: 2304 4742 2304
Size: 2438 5000
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2047 6445 809
Size: 507 731 4398 1802
Fragments arranged by size:
4398 1802 731 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2362 2589 2820
Size: 54 1425 50 275 18 532 227 231
Cuts at: 2820 3030 3762 4885 5774 7403 8
Size: 210 732 1123 889 1629 43
Fragments arranged by size:
1629 1425 1123 889 732 532 275 231
227 210 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4885 1537
Size: 275 3073 4090
Fragments arranged by size:
4090 3073 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2493 3714 3791 3921 4399 4539 4567 5368
Size: 1140 1221 77 130 478 140 28 801
Cuts at: 5368 1353
Size: 3423
Fragments arranged by size:
3423 1221 1140 801 478 140 130 77
28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3007 3418 3436 3868 6025 6399
Size: 1064 138 1418 411 18 432 2157 374
Cuts at: 6399 6545 387
Size: 146 1280
Fragments arranged by size:
2157 1418 1280 1064 432 411 374 146
138 18
EcoICRI GAG'CTC
Cuts at: 4110 4456 4110
Size: 346 7092
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2052 2089 2573 3577 3644 3790 4025
Size: 393 420 37 484 1004 67 146 235
Cuts at: 4025 4474 5501 6098 6885 7153 1239
Size: 449 1027 597 787 268 1524
Fragments arranged by size:
1524 1027 1004 787 597 484 449 420
393 268 235 146 67 37
EcoNI CCTnn'n_nnAGG
Cuts at: 4347 4347
Size: 7438
EcoO109I rG'GnC_Cy
Cuts at: 2131 3946 3947 3980 4340 2131
Size: 1815 1 33 360 5229
Fragments arranged by size:
5229 1815 360 33 1
EcoRI G'AATT_C
Cuts at: 4747 5125 4747
Size: 378 7060
EcoRV GAT'ATC
Cuts at: 2426 2426
Size: 7438
FspI TGC'GCA
Cuts at: 2924 5944 2924
Size: 3020 4418
FspAI rTGC'GCAy
Cuts at: 2924 2924
Size: 7438
HaeII r_GCGC'y
Cuts at: 1678 4331 6585 1678
Size: 2653 2254 2531
Fragments arranged by size:
2653 2531 2254
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2103 2135 3643 3675 3689
Size: 32 135 32 531 32 1508 32 14
Cuts at: 3689 3721 4648 4680 6079 6111 6153 6185 1373
Size: 32 927 32 1399 32 42 32 2626
Fragments arranged by size:
2626 1508 1399 927 531 135 42 32
32 32 32 32 32 32 32 14
HincII GTy'rAC
Cuts at: 669 1657 1938 2052 4021 4782 5028 669
Size: 988 281 114 1969 761 246 3079
Fragments arranged by size:
3079 1969 988 761 281 246 114
HindIII A'AGCT_T
Cuts at: 748 2192 748
Size: 1444 5994
HpaI GTT'AAC
Cuts at: 5028 5028
Size: 7438
KpnI G_GTAC'C
Cuts at: 4763 4763
Size: 7438
MfeI C'AATT_G
Cuts at: 5037 5037
Size: 7438
MluI A'CGCG_T
Cuts at: 7314 7314
Size: 7438
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2886 3483 3540 4716 6552 6736 1305
Size: 1581 597 57 1176 1836 184 2007
Fragments arranged by size:
2007 1836 1581 1176 597 184 57
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2364 2591 2822 3032 10
Size: 54 1425 343 532 227 231 210 4416
Fragments arranged by size:
4416 1425 532 343 231 227 210 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3121 3137 3609 4028 5396 5755 5914
Size: 648 1955 16 472 419 1368 359 159
Cuts at: 5914 518
Size: 2042
Fragments arranged by size:
2042 1955 1368 648 472 419 359 159
16
MspA1I CmG'CkG
Cuts at: 1606 3574 3760 3828 3865 4229 4854 5531 6679
Size: 1968 186 68 37 364 625 677 1148
Cuts at: 6679 6924 1606
Size: 245 2120
Fragments arranged by size:
2120 1968 1148 677 625 364 245 186
68 37
NcoI C'CATG_G
Cuts at: 513 1065 2004 2739 3033 513
Size: 552 939 735 294 4918
Fragments arranged by size:
4918 939 735 552 294
NdeI CA'TA_TG
Cuts at: 387 2331 3712 387
Size: 1944 1381 4113
Fragments arranged by size:
4113 1944 1381
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7438
NotI GC'GGCC_GC
Cuts at: 4885 4885
Size: 7438
NspI r_CATG'y
Cuts at: 1132 1229 2103 4796 6341 1132
Size: 97 874 2693 1545 2229
Fragments arranged by size:
2693 2229 1545 874 97
PasI CC'CwG_GG
Cuts at: 1348 3917 4291 4460 1348
Size: 2569 374 169 4326
Fragments arranged by size:
4326 2569 374 169
PciI A'CATG_T
Cuts at: 6337 6337
Size: 7438
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2335 3622 1108
Size: 1227 1287 4924
Fragments arranged by size:
4924 1287 1227
PfoI T'CCnGG_A
Cuts at: 2105 2223 2281 2105
Size: 118 58 7262
Fragments arranged by size:
7262 118 58
PmeI GTTT'AAAC
Cuts at: 4742 4742
Size: 7438
PmlI CAC'GTG
Cuts at: 2710 4724 2710
Size: 2014 5424
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5507 5539 7020 7052 5507
Size: 32 1481 32 5893
Fragments arranged by size:
5893 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3980 4340 3980
Size: 360 7078
PshAI GACnn'nnGTC
Cuts at: 1613 3779 4581 4638 1613
Size: 2166 802 57 4413
Fragments arranged by size:
4413 2166 802 57
PsiI TTA'TAA
Cuts at: 5008 5008
Size: 7438
PspOMI G'GGCC_C
Cuts at: 3946 3946
Size: 7438
PstI C_TGCA'G
Cuts at: 830 2661 2880 2970 4790 830
Size: 1831 219 90 1820 3478
Fragments arranged by size:
3478 1831 1820 219 90
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7438
PvuII CAG'CTG
Cuts at: 3574 3760 3828 4229 3574
Size: 186 68 401 6783
Fragments arranged by size:
6783 401 186 68
RsrII CG'GwC_CG
Cuts at: 2316 2316
Size: 7438
SacI G_AGCT'C
Cuts at: 4112 4458 4112
Size: 346 7092
SalI G'TCGA_C
Cuts at: 1936 2050 4780 1936
Size: 114 2730 4594
Fragments arranged by size:
4594 2730 114
SapI GCTCTTCn'nnn_
Cuts at: 3791 3921 4567 3791
Size: 130 646 6662
Fragments arranged by size:
6662 646 130
SbfI CC_TGCA'GG
Cuts at: 4790 4790
Size: 7438
ScaI AGT'ACT
Cuts at: 5686 7398 5686
Size: 1712 5726
SfcI C'TryA_G
Cuts at: 826 1045 2657 2876 2966 4591 4786 5921 6602
Size: 219 1612 219 90 1625 195 1135 681
Cuts at: 6602 6793 826
Size: 191 1471
Fragments arranged by size:
1625 1612 1471 1135 681 219 219 195
191 90
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7438
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7438
SmaI CCC'GGG
Cuts at: 1872 2025 4765 1872
Size: 153 2740 4545
Fragments arranged by size:
4545 2740 153
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3323 5542 6443 6705 820
Size: 197 926 1380 2219 901 262 1553
Fragments arranged by size:
2219 1553 1380 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5229 493
Size: 4736 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7438
SphI G_CATG'C
Cuts at: 1132 4796 1132
Size: 3664 3774
SspI AAT'ATT
Cuts at: 5 52 5362 5
Size: 47 5310 2081
Fragments arranged by size:
5310 2081 47
StuI AGG'CCT
Cuts at: 1517 2964 1517
Size: 1447 5991
StyI C'CwwG_G
Cuts at: 513 1065 2004 2739 3033 4877 513
Size: 552 939 735 294 1844 3074
Fragments arranged by size:
3074 1844 939 735 552 294
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3684 3791 5465 5482 5635 5820 6265
Size: 1255 2104 107 1674 17 153 185 445
Cuts at: 6265 325
Size: 1498
Fragments arranged by size:
2104 1674 1498 1255 445 185 153 107
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5684 7396 96
Size: 274 80 33 51 1443 3707 1712 138
Fragments arranged by size:
3707 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2330 2362 2705 2737 4084 4116 7398 7430 2330
Size: 32 343 32 1347 32 3282 32 2338
Fragments arranged by size:
3282 2338 1347 343 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2857 1316
Size: 1541 5897
XbaI T'CTAG_A
Cuts at: 4774 4774
Size: 7438
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2182 2182
Size: 7438
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7438
XmaI C'CCGG_G
Cuts at: 1870 2023 4763 1870
Size: 153 2740 4545
Fragments arranged by size:
4545 2740 153
XmnI GAAnn'nnTTC
Cuts at: 2786 4382 5567 2786
Size: 1596 1185 4657
Fragments arranged by size:
4657 1596 1185
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5246 276
Size: 53 83 186 936 3712 2468
Fragments arranged by size:
3712 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI FspAI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I DraIII FalI FseI KasI NaeI
NarI NgoMIV NruI NsiI PacI PspXI PsrI SacII
SanDI SexAI SfiI SfoI SrfI SwaI