Restriction map for
pFN21AB7307
(Circular) (Six-base) MAPSORT of: pFN21AB7307.seq Check: 2175 from: 1 to: 9823
pFN21AB7307 9823 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3116 3116
Size: 9823
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7633 278
Size: 53 83 186 936 6097 2468
Fragments arranged by size:
6097 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3921 7166 1937
Size: 1984 3245 4594
Fragments arranged by size:
4594 3245 1984
Acc65I G'GTAC_C
Cuts at: 4900 7144 4900
Size: 2244 7579
AclI AA'CG_TT
Cuts at: 1563 7950 8323 1563
Size: 6387 373 3063
Fragments arranged by size:
6387 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2465 3040 3285 6558 7886 9270 9538 1632
Size: 833 575 245 3273 1328 1384 268 1917
Fragments arranged by size:
3273 1917 1384 1328 833 575 268 245
AflII C'TTAA_G
Cuts at: 820 1017 2674 820
Size: 197 1657 7969
Fragments arranged by size:
7969 1657 197
AflIII A'CryG_T
Cuts at: 1203 3743 8722 9699 1203
Size: 2540 4979 977 1327
Fragments arranged by size:
4979 2540 1327 977
AgeI A'CCGG_T
Cuts at: 8654 8654
Size: 9823
AhdI GACnn_n'nnGTC
Cuts at: 5117 8552 5117
Size: 3435 6388
AleI CACnn'nnGTG
Cuts at: 1166 3057 1166
Size: 1891 7932
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9823
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6583 6615 6583
Size: 32 9791
AlwNI CAG_nnn'CTG
Cuts at: 2414 2604 6335 6728 7566 9138 2414
Size: 190 3731 393 838 1572 3099
Fragments arranged by size:
3731 3099 1572 838 393 190
ApaLI G'TGCA_C
Cuts at: 6797 7880 9036 6797
Size: 1083 1156 7584
Fragments arranged by size:
7584 1156 1083
ApoI r'AATT_y
Cuts at: 1493 2087 2166 2417 2466 2552 3187 3574 4007
Size: 594 79 251 49 86 635 387 433
Cuts at: 4007 4332 4877 5154 5309 5444 5699 5879 6087
Size: 325 545 277 155 135 255 180 208
Cuts at: 6087 6348 6535 7132 7358 7510 9619 1493
Size: 261 187 597 226 152 2109 1697
Fragments arranged by size:
2109 1697 635 597 594 545 433 387
325 277 261 255 251 226 208 187
180 155 152 135 86 79 49
AseI AT'TA_AT
Cuts at: 160 1032 2910 2979 8377 160
Size: 872 1878 69 5398 1606
Fragments arranged by size:
5398 1878 1606 872 69
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9823
AvaI C'yCGr_G
Cuts at: 1870 1943 2274 7148 1870
Size: 73 331 4874 4545
Fragments arranged by size:
4874 4545 331 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5661 5694 5661
Size: 33 9790
BamHI G'GATC_C
Cuts at: 7153 7520 7153
Size: 367 9456
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2991 4900 5375 6718 7144
Size: 325 217 627 1204 1909 475 1343 426
Cuts at: 7144 8599 618
Size: 1455 1842
Fragments arranged by size:
1909 1842 1455 1343 1204 627 475 426
325 217
BanII G_rGCy'C
Cuts at: 1391 4987 5638 6524 6953 7142 1391
Size: 3596 651 886 429 189 4072
Fragments arranged by size:
4072 3596 886 651 429 189
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2337 3621 928
Size: 971 438 1284 7130
Fragments arranged by size:
7130 1284 971 438
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4034 4068 8014 8048 1903
Size: 34 2097 34 3946 34 3678
Fragments arranged by size:
3946 3678 2097 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2055 4772 6331 7715 8925 2055
Size: 2717 1559 1384 1210 2953
Fragments arranged by size:
2953 2717 1559 1384 1210
BclI T'GATC_A
Cuts at: 1548 3364 1548
Size: 1816 8007
BfrBI ATG'CAT
Cuts at: 6650 6650
Size: 9823
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5373 8434 136
Size: 107 122 71 631 605 3701 3061 1525
Fragments arranged by size:
3701 3061 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 9818 9818
Size: 9823
BlpI GC'TnA_GC
Cuts at: 7240 7240
Size: 9823
Bme1580I G_kGCm'C
Cuts at: 1790 2994 6801 7884 9040 1790
Size: 1204 3807 1083 1156 2573
Fragments arranged by size:
3807 2573 1204 1156 1083
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9823
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4321 7554 8512
Size: 416 53 83 399 24 2904 3233 958
Cuts at: 8512 442
Size: 1753
Fragments arranged by size:
3233 2904 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9823
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4854 4854
Size: 9823
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2228 2547 2709 3454 4215 4635 4971 5076
Size: 989 319 162 745 761 420 336 105
Cuts at: 5076 5472 6585 6876 8483 1239
Size: 396 1113 291 1607 2579
Fragments arranged by size:
2579 1607 1113 989 761 745 420 396
336 319 291 162 105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4190 5587 5658 5772 6867 7005 7948 8813 9111
Size: 1397 71 114 1095 138 943 865 298
Cuts at: 9111 4190
Size: 4902
Fragments arranged by size:
4902 1397 1095 943 865 298 138 114
71
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4839 8486 882
Size: 617 3340 3647 2219
Fragments arranged by size:
3647 3340 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 7614 493
Size: 711 6410 2702
Fragments arranged by size:
6410 2702 711
BsaBI GATnn'nnATC
Cuts at: 2586 3042 9817 2586
Size: 456 6775 2592
Fragments arranged by size:
6775 2592 456
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7630 8012 8647 275
Size: 53 83 186 936 6097 382 635 1451
Fragments arranged by size:
6097 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 8256 8654 8928 9075 9486 8256
Size: 398 274 147 411 8593
Fragments arranged by size:
8593 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2201 2231 6583 6613 1178
Size: 30 165 30 798 30 4352 30 4388
Fragments arranged by size:
4388 4352 798 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2262 4910 1186
Size: 1076 2648 6099
Fragments arranged by size:
6099 2648 1076
BseYI C'CCAG_C
Cuts at: 4588 6302 6624 6776 9026 9522 4588
Size: 1714 322 152 2250 496 4889
Fragments arranged by size:
4889 2250 1714 496 322 152
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4594 5796 6480 1163
Size: 573 2858 1202 684 4506
Fragments arranged by size:
4506 2858 1202 684 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7273 8034 9062 1540
Size: 275 186 5272 761 1028 2301
Fragments arranged by size:
5272 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2135 2725 3054 4987 6801 6953 7884 7969 9040
Size: 590 329 1933 1814 152 931 85 1071
Cuts at: 9040 2135
Size: 2918
Fragments arranged by size:
2918 1933 1814 1071 931 590 329 152
85
BsmI GAATG_Cn'
Cuts at: 4674 6372 7334 7427 4674
Size: 1698 962 93 7070
Fragments arranged by size:
7070 1698 962 93
BsmBI CGTCTCn'nnnn_
Cuts at: 6687 6687
Size: 9823
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2135 2725 2994 3054 4987 5638 6524
Size: 399 345 590 269 60 1933 651 886
Cuts at: 6524 6801 6953 7142 7884 7969 9040 1391
Size: 277 152 189 742 85 1071 2174
Fragments arranged by size:
2174 1933 1071 886 742 651 590 399
345 277 269 189 152 85 60
BspEI T'CCGG_A
Cuts at: 9486 9486
Size: 9823
BspHI T'CATG_A
Cuts at: 2584 2653 2914 7712 9442 2584
Size: 69 261 4798 1730 2965
Fragments arranged by size:
4798 2965 1730 261 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3116 6811 6997 7178 844
Size: 2272 3695 186 181 3489
Fragments arranged by size:
3695 3489 2272 186 181
BsrBI CCG'CTC
Cuts at: 1394 1823 2041 7710 1394
Size: 429 218 5669 3507
Fragments arranged by size:
5669 3507 429 218
BsrDI GCAATG_nn'
Cuts at: 65 1248 8318 8492 65
Size: 1183 7070 174 1396
Fragments arranged by size:
7070 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 8467 8654 1700
Size: 110 6657 187 2869
Fragments arranged by size:
6657 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3881 5587 96
Size: 3785 1706 4332
Fragments arranged by size:
4332 3785 1706
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9823
BssSI C'ACGA_G
Cuts at: 7883 8895 7883
Size: 1012 8811
BstAPI GCAn_nnn'nTGC
Cuts at: 794 6503 794
Size: 5709 4114
BstBI TT'CG_AA
Cuts at: 8627 8627
Size: 9823
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9823
BstXI CCAn_nnnn'nTGG
Cuts at: 2297 6772 2297
Size: 4475 5348
BstYI r'GATC_y
Cuts at: 1972 2031 2561 4823 5729 6309 7153 7520 7905
Size: 59 530 2262 906 580 844 367 385
Cuts at: 7905 7922 9374 9460 9472 9818 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2262 1977 1452 906 844 580 530 385
367 346 86 59 17 12
Bsu36I CC'TnA_GG
Cuts at: 6526 6526
Size: 9823
BtgI C'CryG_G
Cuts at: 513 1065 2004 3735 3845 6063 6704 6771 6941
Size: 552 939 1731 110 2218 641 67 170
Cuts at: 6941 513
Size: 3395
Fragments arranged by size:
3395 2218 1731 939 641 552 170 110
67
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9084
Fragments arranged by size:
9084 683 56
BtsI GCAGTG_nn'
Cuts at: 2921 7339 8133 8153 2921
Size: 4418 794 20 4591
Fragments arranged by size:
4591 4418 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9823
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9788
DraI TTT'AAA
Cuts at: 2818 3652 4869 5884 7127 2818
Size: 834 1217 1015 1243 5514
Fragments arranged by size:
5514 1243 1217 1015 834
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8830 809
Size: 507 7514 1802
Fragments arranged by size:
7514 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2424 3848 4755
Size: 54 1425 50 275 18 594 1424 907
Cuts at: 4755 7270 8159 9788 8
Size: 2515 889 1629 43
Fragments arranged by size:
2515 1629 1425 1424 907 889 594 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7270 1537
Size: 275 5458 4090
Fragments arranged by size:
5458 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2373 2934 3762 4813 5883 6030 6169 7066
Size: 1020 561 828 1051 1070 147 139 897
Cuts at: 7066 7753 1353
Size: 687 3423
Fragments arranged by size:
3423 1070 1051 1020 897 828 687 561
147 139
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8410 8784 8930 387
Size: 1064 138 6821 374 146 1280
Fragments arranged by size:
6821 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4985 6951 4985
Size: 1966 7857
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2228 2465 2547 2709 3040 3285 3454
Size: 393 596 237 82 162 331 245 169
Cuts at: 3454 4215 4635 4971 5076 5472 6558 6585 6876
Size: 761 420 336 105 396 1086 27 291
Cuts at: 6876 7886 8483 9270 9538 1239
Size: 1010 597 787 268 1524
Fragments arranged by size:
1524 1086 1010 787 761 597 596 420
396 393 336 331 291 268 245 237
169 162 105 82 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2529 3761 5481 6391 2529
Size: 1232 1720 910 5961
Fragments arranged by size:
5961 1720 1232 910
EcoRI G'AATT_C
Cuts at: 5699 7132 7510 5699
Size: 1433 378 8012
Fragments arranged by size:
8012 1433 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4234 6515 4234
Size: 2281 7542
FspI TGC'GCA
Cuts at: 8329 8329
Size: 9823
HaeII r_GCGC'y
Cuts at: 1678 8970 1678
Size: 7292 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2484 2516 3329 3361 4079
Size: 32 135 32 912 32 813 32 718
Cuts at: 4079 4111 5103 5135 5364 5396 5903 5935 6029
Size: 32 992 32 229 32 507 32 94
Cuts at: 6029 6061 6938 6970 8464 8496 8538 8570 1373
Size: 32 877 32 1494 32 42 32 2626
Fragments arranged by size:
2626 1494 992 912 877 813 718 507
229 135 94 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3337 5460 5559 7050 7167 7413
Size: 988 281 1399 2123 99 1491 117 246
Cuts at: 7413 669
Size: 3079
Fragments arranged by size:
3079 2123 1491 1399 988 281 246 117
99
HindIII A'AGCT_T
Cuts at: 748 4244 748
Size: 3496 6327
HpaI GTT'AAC
Cuts at: 5460 7413 5460
Size: 1953 7870
KpnI G_GTAC'C
Cuts at: 4904 7148 4904
Size: 2244 7579
MfeI C'AATT_G
Cuts at: 7422 7422
Size: 9823
MluI A'CGCG_T
Cuts at: 9699 9699
Size: 9823
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2418 3179 4053 4903 6343 6945 8937 9121
Size: 1113 761 874 850 1440 602 1992 184
Cuts at: 9121 1305
Size: 2007
Fragments arranged by size:
2007 1992 1440 1113 874 850 761 602
184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2426 3850 4757 10
Size: 54 1425 343 594 1424 907 5076
Fragments arranged by size:
5076 1425 1424 907 594 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2142 3057 6126 6770 7781 8140 8299
Size: 648 976 915 3069 644 1011 359 159
Cuts at: 8299 518
Size: 2042
Fragments arranged by size:
3069 2042 1011 976 915 648 644 359
159
MspA1I CmG'CkG
Cuts at: 1606 5585 6578 7239 7916 9064 9309 1606
Size: 3979 993 661 677 1148 245 2120
Fragments arranged by size:
3979 2120 1148 993 677 661 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3735 3845 6063 6704 6771 513
Size: 552 939 1731 110 2218 641 67 3565
Fragments arranged by size:
3565 2218 1731 939 641 552 110 67
NdeI CA'TA_TG
Cuts at: 387 2944 387
Size: 2557 7266
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9823
NotI GC'GGCC_GC
Cuts at: 7270 7270
Size: 9823
NsiI A_TGCA'T
Cuts at: 6652 6652
Size: 9823
NspI r_CATG'y
Cuts at: 1132 1229 3378 3591 3747 7181 8726 1132
Size: 97 2149 213 156 3434 1545 2229
Fragments arranged by size:
3434 2229 2149 1545 213 156 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9823
PciI A'CATG_T
Cuts at: 3743 8722 3743
Size: 4979 4844
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2966 6620 6728 1108
Size: 1858 3654 108 4203
Fragments arranged by size:
4203 3654 1858 108
PfoI T'CCnGG_A
Cuts at: 2524 3431 4231 5449 2524
Size: 907 800 1218 6898
Fragments arranged by size:
6898 1218 907 800
PmeI GTTT'AAAC
Cuts at: 7127 7127
Size: 9823
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2670 2702 5015 5047 6583 6615 7892 7924 9405
Size: 32 2313 32 1536 32 1277 32 1481
Cuts at: 9405 9437 2670
Size: 32 3056
Fragments arranged by size:
3056 2313 1536 1481 1277 32 32 32
32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9823
PsiI TTA'TAA
Cuts at: 7393 7393
Size: 9823
PspXI vC'TCGA_Gb
Cuts at: 2274 2274
Size: 9823
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2932 2964 2932
Size: 32 9791
PstI C_TGCA'G
Cuts at: 830 4387 4951 6822 7175 830
Size: 3557 564 1871 353 3478
Fragments arranged by size:
3557 3478 1871 564 353
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9823
PvuII CAG'CTG
Cuts at: 5585 6578 5585
Size: 993 8830
SacI G_AGCT'C
Cuts at: 4987 6953 4987
Size: 1966 7857
SalI G'TCGA_C
Cuts at: 1936 7165 1936
Size: 5229 4594
SapI GCTCTTCn'nnn_
Cuts at: 6030 6030
Size: 9823
SbfI CC_TGCA'GG
Cuts at: 7175 7175
Size: 9823
ScaI AGT'ACT
Cuts at: 4973 5125 8071 9783 4973
Size: 152 2946 1712 5013
Fragments arranged by size:
5013 2946 1712 152
SexAI A'CCwGG_T
Cuts at: 5476 5476
Size: 9823
SfcI C'TryA_G
Cuts at: 826 1045 2256 2373 4383 4947 6818 7171 8306
Size: 219 1211 117 2010 564 1871 353 1135
Cuts at: 8306 8987 9178 826
Size: 681 191 1471
Fragments arranged by size:
2010 1871 1471 1211 1135 681 564 353
219 191 117
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9823
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9823
SmaI CCC'GGG
Cuts at: 1872 7150 1872
Size: 5278 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2274 2674 4169 5602 5673 5787
Size: 197 926 331 400 1495 1433 71 114
Cuts at: 5787 6882 7020 7927 8828 9090 820
Size: 1095 138 907 901 262 1553
Fragments arranged by size:
1553 1495 1433 1095 926 907 901 400
331 262 197 138 114 71
SnaBI TAC'GTA
Cuts at: 493 7614 493
Size: 7121 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9823
SphI G_CATG'C
Cuts at: 1132 3591 7181 1132
Size: 2459 3590 3774
Fragments arranged by size:
3774 3590 2459
SspI AAT'ATT
Cuts at: 5 52 3635 4724 7747 5
Size: 47 3583 1089 3023 2081
Fragments arranged by size:
3583 3023 2081 1089 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9823
StyI C'CwwG_G
Cuts at: 513 1065 2004 3735 3845 4765 5508 6063 6704
Size: 552 939 1731 110 920 743 555 641
Cuts at: 6704 6771 7262 513
Size: 67 491 3074
Fragments arranged by size:
3074 1731 939 920 743 641 555 552
491 110 67
SwaI ATTT'AAAT
Cuts at: 2818 2818
Size: 9823
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 7850 7867 8020 8205 8650 325
Size: 1255 6270 17 153 185 445 1498
Fragments arranged by size:
6270 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3491 3553 3881
Size: 274 80 33 51 1443 1514 62 328
Cuts at: 3881 4324 4734 4971 5123 5416 5587 6001 8069
Size: 443 410 237 152 293 171 414 2068
Cuts at: 8069 9781 96
Size: 1712 138
Fragments arranged by size:
2068 1712 1514 1443 443 414 410 328
293 274 237 171 152 138 80 62
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3486 3518 6272 6304 6702 6734 9783 9815 3486
Size: 32 2754 32 398 32 3049 32 3494
Fragments arranged by size:
3494 3049 2754 398 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2604 1316
Size: 1288 8535
XbaI T'CTAG_A
Cuts at: 4450 7159 4450
Size: 2709 7114
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2522 6621 2522
Size: 4099 5724
XhoI C'TCGA_G
Cuts at: 1943 2274 1943
Size: 331 9492
XmaI C'CCGG_G
Cuts at: 1870 7148 1870
Size: 5278 4545
XmnI GAAnn'nnTTC
Cuts at: 2243 2367 2685 3543 4959 5082 5445 6352 6737
Size: 124 318 858 1416 123 363 907 385
Cuts at: 6737 7952 2243
Size: 1215 4114
Fragments arranged by size:
4114 1416 1215 907 858 385 363 318
124 123
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7631 276
Size: 53 83 186 936 6097 2468
Fragments arranged by size:
6097 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspXI PsrI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbeI BbvCI Bpu10I BsiWI
BstZ17I DraIII EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PpuMI PspOMI RsrII
SacII SanDI SfiI SfoI SrfI