Restriction map for
pFN21AB7305
(Circular) (Six-base) MAPSORT of: pFN21AB7305.seq Check: 4814 from: 1 to: 7135
pFN21AB7305 7135 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4945 278
Size: 53 83 186 936 3409 2468
Fragments arranged by size:
3409 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4478 1937
Size: 2541 4594
Acc65I G'GTAC_C
Cuts at: 4456 4456
Size: 7135
AclI AA'CG_TT
Cuts at: 1563 5262 5635 1563
Size: 3699 373 3063
Fragments arranged by size:
3699 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3068 3509 5198 6582 6850 1632
Size: 1436 441 1689 1384 268 1917
Fragments arranged by size:
1917 1689 1436 1384 441 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6938
AflIII A'CryG_T
Cuts at: 1203 6034 7011 1203
Size: 4831 977 1327
Fragments arranged by size:
4831 1327 977
AgeI A'CCGG_T
Cuts at: 5966 5966
Size: 7135
AhdI GACnn_n'nnGTC
Cuts at: 3596 5864 3596
Size: 2268 4867
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7135
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7135
AlwNI CAG_nnn'CTG
Cuts at: 4878 6450 4878
Size: 1572 5563
ApaLI G'TGCA_C
Cuts at: 2538 5192 6348 2538
Size: 2654 1156 3325
Fragments arranged by size:
3325 2654 1156
ApoI r'AATT_y
Cuts at: 1493 4444 4670 4822 6931 1493
Size: 2951 226 152 2109 1697
Fragments arranged by size:
2951 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2813 5689 160
Size: 872 1781 2876 1606
Fragments arranged by size:
2876 1781 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7135
AvaI C'yCGr_G
Cuts at: 1870 1943 4402 4460 1870
Size: 73 2459 58 4545
Fragments arranged by size:
4545 2459 73 58
BamHI G'GATC_C
Cuts at: 4465 4832 4465
Size: 367 6768
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4128 4456 5911 618
Size: 325 217 627 2341 328 1455 1842
Fragments arranged by size:
2341 1842 1455 627 328 325 217
BanII G_rGCy'C
Cuts at: 1391 2090 2919 4125 4454 1391
Size: 699 829 1206 329 4072
Fragments arranged by size:
4072 1206 829 699 329
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4353 928
Size: 971 2454 3710
Fragments arranged by size:
3710 2454 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5326 5360 1903
Size: 34 3389 34 3678
Fragments arranged by size:
3678 3389 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2487 5027 6237 2487
Size: 2540 1210 3385
Fragments arranged by size:
3385 2540 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7135
BfrBI ATG'CAT
Cuts at: 2963 2963
Size: 7135
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4137 4146 5746
Size: 107 122 71 631 605 2465 9 1600
Cuts at: 5746 136
Size: 1525
Fragments arranged by size:
2465 1600 1525 631 605 122 107 71
9
BglII A'GATC_T
Cuts at: 2315 7130 2315
Size: 4815 2320
BlpI GC'TnA_GC
Cuts at: 2084 4011 4552 2084
Size: 1927 541 4667
Fragments arranged by size:
4667 1927 541
Bme1580I G_kGCm'C
Cuts at: 1790 2542 5196 6352 1790
Size: 752 2654 1156 2573
Fragments arranged by size:
2654 2573 1156 752
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7135
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3654 4866 5824
Size: 416 53 83 399 24 2237 1212 958
Cuts at: 5824 442
Size: 1753
Fragments arranged by size:
2237 1753 1212 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7135
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2109 2109
Size: 7135
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4379 5795 1239
Size: 3140 1416 2579
Fragments arranged by size:
3140 2579 1416
Bpu10I CC'TnA_GC
Cuts at: 4124 4124
Size: 7135
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5260 6125 6423 5260
Size: 865 298 5972
Fragments arranged by size:
5972 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4355 5798 882
Size: 617 2856 1443 2219
Fragments arranged by size:
2856 2219 1443 617
BsaAI yAC'GTr
Cuts at: 493 1204 4926 493
Size: 711 3722 2702
Fragments arranged by size:
3722 2702 711
BsaBI GATnn'nnATC
Cuts at: 7129 7129
Size: 7135
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4942 5324 5959 275
Size: 53 83 186 936 3409 382 635 1451
Fragments arranged by size:
3409 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5568 5966 6240 6387 6798 5568
Size: 398 274 147 411 5905
Fragments arranged by size:
5905 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6910
Fragments arranged by size:
6910 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7135
BseYI C'CCAG_C
Cuts at: 6338 6834 6338
Size: 496 6639
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3846 3869 1163
Size: 573 2110 23 4429
Fragments arranged by size:
4429 2110 573 23
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4585 5346 6374 1540
Size: 275 186 2584 761 1028 2301
Fragments arranged by size:
2584 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2090 2542 2919 4125 5196 5281 6352 2090
Size: 452 377 1206 1071 85 1071 2873
Fragments arranged by size:
2873 1206 1071 1071 452 377 85
BsmI GAATG_Cn'
Cuts at: 3929 4646 4739 3929
Size: 717 93 6325
Fragments arranged by size:
6325 717 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2090 2542 2919 4125 4454 5196 5281
Size: 399 300 452 377 1206 329 742 85
Cuts at: 5281 6352 1391
Size: 1071 2174
Fragments arranged by size:
2174 1206 1071 742 452 399 377 329
300 85
BspEI T'CCGG_A
Cuts at: 6798 6798
Size: 7135
BspHI T'CATG_A
Cuts at: 4117 5024 6754 4117
Size: 907 1730 4498
Fragments arranged by size:
4498 1730 907
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4490 844
Size: 3646 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4396 5022 1394
Size: 429 2573 626 3507
Fragments arranged by size:
3507 2573 626 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2619 3537 4005 5630 5804 65
Size: 1183 1371 918 468 1625 174 1396
Fragments arranged by size:
1625 1396 1371 1183 918 468 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5779 5966 1700
Size: 110 3969 187 2869
Fragments arranged by size:
3969 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2923 96
Size: 2827 4308
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7135
BssSI C'ACGA_G
Cuts at: 5195 6207 5195
Size: 1012 6123
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7135
BstBI TT'CG_AA
Cuts at: 3808 5939 3808
Size: 2131 5004
BstEII G'GTnAC_C
Cuts at: 1184 2008 1184
Size: 824 6311
BstXI CCAn_nnnn'nTGG
Cuts at: 2149 4082 2149
Size: 1933 5202
BstYI r'GATC_y
Cuts at: 1972 2315 4465 4832 5217 5234 6686 6772 6784
Size: 343 2150 367 385 17 1452 86 12
Cuts at: 6784 7130 1972
Size: 346 1977
Fragments arranged by size:
2150 1977 1452 385 367 346 343 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5644
Fragments arranged by size:
5644 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6396
Fragments arranged by size:
6396 683 56
BtsI GCAGTG_nn'
Cuts at: 2542 4323 4651 5445 5465 2542
Size: 1781 328 794 20 4212
Fragments arranged by size:
4212 1781 794 328 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7135
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7100
DraI TTT'AAA
Cuts at: 2845 3321 4439 2845
Size: 476 1118 5541
Fragments arranged by size:
5541 1118 476
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6142 809
Size: 507 4826 1802
Fragments arranged by size:
4826 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2139 4582 5471
Size: 54 1425 50 275 18 309 2443 889
Cuts at: 5471 7100 8
Size: 1629 43
Fragments arranged by size:
2443 1629 1425 889 309 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4582 1537
Size: 275 2770 4090
Fragments arranged by size:
4090 2770 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2353 3270 3476 3805 4405 5065 1353
Size: 1000 917 206 329 600 660 3423
Fragments arranged by size:
3423 1000 917 660 600 329 206
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5722 6096 6242 387
Size: 1064 138 4133 374 146 1280
Fragments arranged by size:
4133 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2088 2917 4123 2088
Size: 829 1206 5100
Fragments arranged by size:
5100 1206 829
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3068 3509 4379 5198 5795 6582 6850
Size: 393 1436 441 870 819 597 787 268
Cuts at: 6850 1239
Size: 1524
Fragments arranged by size:
1524 1436 870 819 787 597 441 393
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2672 2672
Size: 7135
EcoRI G'AATT_C
Cuts at: 4444 4822 4444
Size: 378 6757
EcoRV GAT'ATC
Cuts at: 3031 3031
Size: 7135
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2684 3896 4058 4070 2684
Size: 1212 162 12 5749
Fragments arranged by size:
5749 1212 162 12
FspI TGC'GCA
Cuts at: 5641 5641
Size: 7135
HaeII r_GCGC'y
Cuts at: 1678 3628 6282 1678
Size: 1950 2654 2531
Fragments arranged by size:
2654 2531 1950
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2128 2160 2291 2323 2485
Size: 32 135 32 556 32 131 32 162
Cuts at: 2485 2517 3582 3614 4196 4228 5776 5808 5850
Size: 32 1065 32 582 32 1548 32 42
Cuts at: 5850 5882 1373
Size: 32 2626
Fragments arranged by size:
2626 1548 1065 582 556 162 135 131
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4189 4479 4725 669
Size: 988 281 2251 290 246 3079
Fragments arranged by size:
3079 2251 988 290 281 246
HindIII A'AGCT_T
Cuts at: 748 2790 3233 4040 748
Size: 2042 443 807 3843
Fragments arranged by size:
3843 2042 807 443
HpaI GTT'AAC
Cuts at: 4725 4725
Size: 7135
KpnI G_GTAC'C
Cuts at: 4460 4460
Size: 7135
MfeI C'AATT_G
Cuts at: 3009 4734 3009
Size: 1725 5410
MluI A'CGCG_T
Cuts at: 7011 7011
Size: 7135
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2737 3387 6249 6433 1305
Size: 1432 650 2862 184 2007
Fragments arranged by size:
2862 2007 1432 650 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2141 10
Size: 54 1425 343 309 5004
Fragments arranged by size:
5004 1425 343 309 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3542 5093 5452 5611 518
Size: 648 2376 1551 359 159 2042
Fragments arranged by size:
2376 2042 1551 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4551 5228 6376 6621 1606
Size: 2945 677 1148 245 2120
Fragments arranged by size:
2945 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5644
Fragments arranged by size:
5644 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7135
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7135
NotI GC'GGCC_GC
Cuts at: 4582 4582
Size: 7135
NsiI A_TGCA'T
Cuts at: 2965 2965
Size: 7135
NspI r_CATG'y
Cuts at: 1132 1229 4493 6038 1132
Size: 97 3264 1545 2229
Fragments arranged by size:
3264 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7135
PciI A'CATG_T
Cuts at: 6034 6034
Size: 7135
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7135
PmeI GTTT'AAAC
Cuts at: 4439 4439
Size: 7135
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5204 5236 6717 6749 5204
Size: 32 1481 32 5590
Fragments arranged by size:
5590 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7135
PsiI TTA'TAA
Cuts at: 4705 4705
Size: 7135
PstI C_TGCA'G
Cuts at: 830 4487 830
Size: 3657 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7135
SacI G_AGCT'C
Cuts at: 2090 2919 4125 2090
Size: 829 1206 5100
Fragments arranged by size:
5100 1206 829
SalI G'TCGA_C
Cuts at: 1936 4477 1936
Size: 2541 4594
SapI GCTCTTCn'nnn_
Cuts at: 2353 3270 3805 2353
Size: 917 535 5683
Fragments arranged by size:
5683 917 535
SbfI CC_TGCA'GG
Cuts at: 4487 4487
Size: 7135
ScaI AGT'ACT
Cuts at: 5383 7095 5383
Size: 1712 5423
SfcI C'TryA_G
Cuts at: 826 1045 3386 3836 4483 5618 6299 6490 826
Size: 219 2341 450 647 1135 681 191 1471
Fragments arranged by size:
2341 1471 1135 681 647 450 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7135
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7135
SmaI CCC'GGG
Cuts at: 1872 4462 1872
Size: 2590 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5239 6140 6402 820
Size: 197 926 3296 901 262 1553
Fragments arranged by size:
3296 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4926 493
Size: 4433 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7135
SphI G_CATG'C
Cuts at: 1132 4493 1132
Size: 3361 3774
SspI AAT'ATT
Cuts at: 5 52 3514 5059 5
Size: 47 3462 1545 2081
Fragments arranged by size:
3462 2081 1545 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7135
StyI C'CwwG_G
Cuts at: 513 1065 2004 2668 4574 513
Size: 552 939 664 1906 3074
Fragments arranged by size:
3074 1906 939 664 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2092 3679 5162 5179 5332 5517 5962
Size: 1255 512 1587 1483 17 153 185 445
Cuts at: 5962 325
Size: 1498
Fragments arranged by size:
1587 1498 1483 1255 512 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2923 5381 7093
Size: 274 80 33 51 1443 946 2458 1712
Cuts at: 7093 96
Size: 138
Fragments arranged by size:
2458 1712 1443 946 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7095 7127 7095
Size: 32 7103
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7135
XbaI T'CTAG_A
Cuts at: 4471 4471
Size: 7135
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7135
XmaI C'CCGG_G
Cuts at: 1870 4460 1870
Size: 2590 4545
XmnI GAAnn'nnTTC
Cuts at: 2481 5264 2481
Size: 2783 4352
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4943 276
Size: 53 83 186 936 3409 2468
Fragments arranged by size:
3409 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI XcmI