Restriction map for
pFN21AB7302
(Circular) (Six-base) MAPSORT of: pFN21AB7302.seq Check: 1545 from: 1 to: 8458
pFN21AB7302 8458 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3145 3397 3145
Size: 252 8206
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3777 5487 6268 278
Size: 53 83 186 936 2241 1710 781 2468
Fragments arranged by size:
2468 2241 1710 936 781 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4950 5801 1937
Size: 3013 851 4594
Fragments arranged by size:
4594 3013 851
Acc65I G'GTAC_C
Cuts at: 5779 5779
Size: 8458
AclI AA'CG_TT
Cuts at: 1563 6585 6958 1563
Size: 5022 373 3063
Fragments arranged by size:
5022 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3189 3276 3312 3591 3873 4223 4949 6521
Size: 1557 87 36 279 282 350 726 1572
Cuts at: 6521 7905 8173 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1572 1557 1384 726 350 282 279
268 87 36
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8261
AflIII A'CryG_T
Cuts at: 1203 4365 7357 8334 1203
Size: 3162 2992 977 1327
Fragments arranged by size:
3162 2992 1327 977
AgeI A'CCGG_T
Cuts at: 7289 7289
Size: 8458
AhdI GACnn_n'nnGTC
Cuts at: 7187 7187
Size: 8458
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8458
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8458
AlwNI CAG_nnn'CTG
Cuts at: 2389 2500 2573 3217 3715 4591 5716 6201 7773
Size: 111 73 644 498 876 1125 485 1572
Cuts at: 7773 2389
Size: 3074
Fragments arranged by size:
3074 1572 1125 876 644 498 485 111
73
ApaI G_GGCC'C
Cuts at: 2266 2619 5407 5696 2266
Size: 353 2788 289 5028
Fragments arranged by size:
5028 2788 353 289
ApaLI G'TGCA_C
Cuts at: 2419 6515 7671 2419
Size: 4096 1156 3206
Fragments arranged by size:
4096 3206 1156
ApoI r'AATT_y
Cuts at: 1493 4417 4717 5767 5993 6145 8254 1493
Size: 2924 300 1050 226 152 2109 1697
Fragments arranged by size:
2924 2109 1697 1050 300 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7012 160
Size: 872 5980 1606
Fragments arranged by size:
5980 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8458
AvaI C'yCGr_G
Cuts at: 1870 1943 2631 3526 5307 5573 5581 5689 5783
Size: 73 688 895 1781 266 8 108 94
Cuts at: 5783 1870
Size: 4545
Fragments arranged by size:
4545 1781 895 688 266 108 94 73
8
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2973 3006 2973
Size: 33 8425
BamHI G'GATC_C
Cuts at: 5788 6155 5788
Size: 367 8091
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2692 5315 5779 7234 618
Size: 325 217 627 905 2623 464 1455 1842
Fragments arranged by size:
2623 1842 1455 905 627 464 325 217
BanII G_rGCy'C
Cuts at: 1391 2266 2619 2709 3315 3579 3797 5407 5477
Size: 875 353 90 606 264 218 1610 70
Cuts at: 5477 5580 5600 5658 5690 5696 5777 1391
Size: 103 20 58 32 6 81 4072
Fragments arranged by size:
4072 1610 875 606 353 264 218 103
90 81 70 58 32 20 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2063 3186 4532 4573 928
Size: 971 164 1123 1346 41 4813
Fragments arranged by size:
4813 1346 1123 971 164 41
BbvCI CC'TCA_GC
Cuts at: 2034 2517 3351 4352 2034
Size: 483 834 1001 6140
Fragments arranged by size:
6140 1001 834 483
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6649 6683 1903
Size: 34 4712 34 3678
Fragments arranged by size:
4712 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6350 7560 6350
Size: 1210 7248
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8458
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2447 3671 7069
Size: 107 122 71 631 605 775 1224 3398
Cuts at: 7069 136
Size: 1525
Fragments arranged by size:
3398 1525 1224 775 631 605 122 107
71
BglII A'GATC_T
Cuts at: 8453 8453
Size: 8458
BlpI GC'TnA_GC
Cuts at: 3520 4232 5875 3520
Size: 712 1643 6103
Fragments arranged by size:
6103 1643 712
Bme1580I G_kGCm'C
Cuts at: 1790 2266 2423 2619 5318 5407 5696 6519 7675
Size: 476 157 196 2699 89 289 823 1156
Cuts at: 7675 1790
Size: 2573
Fragments arranged by size:
2699 2573 1156 823 476 289 196 157
89
BmgBI CAC'GTC
Cuts at: 1318 4858 1318
Size: 3540 4918
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2415 3331 3599
Size: 416 53 83 399 24 998 916 268
Cuts at: 3599 4444 4480 6189 7147 442
Size: 845 36 1709 958 1753
Fragments arranged by size:
1753 1709 998 958 916 845 416 399
268 83 53 36 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8458
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2659 2866 5518 2659
Size: 207 2652 5599
Fragments arranged by size:
5599 2652 207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2510 2563 2577 2685 2813 2913 3692 3819
Size: 1271 53 14 108 128 100 779 127
Cuts at: 3819 5450 7118 1239
Size: 1631 1668 2579
Fragments arranged by size:
2579 1668 1631 1271 779 128 127 108
100 53 14
Bpu10I CC'TnA_GC
Cuts at: 2034 2517 3351 4352 2034
Size: 483 834 1001 6140
Fragments arranged by size:
6140 1001 834 483
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2465 3563 6583 7448 7746 2465
Size: 1098 3020 865 298 3177
Fragments arranged by size:
3177 3020 1098 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2216 2630 3323 5592 7121 882
Size: 617 717 414 693 2269 1529 2219
Fragments arranged by size:
2269 2219 1529 717 693 617 414
BsaAI yAC'GTr
Cuts at: 493 1204 4366 6249 493
Size: 711 3162 1883 2702
Fragments arranged by size:
3162 2702 1883 711
BsaBI GATnn'nnATC
Cuts at: 5003 8452 5003
Size: 3449 5009
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2599 3774 4608 5484
Size: 53 83 186 936 1066 1175 834 876
Cuts at: 5484 5567 6265 6647 7282 275
Size: 83 698 382 635 1451
Fragments arranged by size:
1451 1175 1066 936 876 834 698 635
382 186 83 83 53
BsaWI w'CCGG_w
Cuts at: 5232 6891 7289 7563 7710 8121 5232
Size: 1659 398 274 147 411 5569
Fragments arranged by size:
5569 1659 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2886 2916 1178
Size: 30 165 30 1483 30 6720
Fragments arranged by size:
6720 1483 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2150 2201 2725 2939 3074 3089 3092 3107
Size: 964 51 524 214 135 15 3 15
Cuts at: 3107 3110 3113 3116 3125 3128 3251 3287 3296
Size: 3 3 3 9 3 123 36 9
Cuts at: 3296 3311 3353 3803 3887 4274 5294 5651 5654
Size: 15 42 450 84 387 1020 357 3
Cuts at: 5654 5759 1186
Size: 105 3885
Fragments arranged by size:
3885 1020 964 524 450 387 357 214
135 123 105 84 51 42 36 15
15 15 9 9 3 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 2013 2297 2584 2709 3808 4035 7661 8157 2013
Size: 284 287 125 1099 227 3626 496 2314
Fragments arranged by size:
3626 2314 1099 496 287 284 227 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2768 2891 3425 4763 4838 5045 1163
Size: 573 1032 123 534 1338 75 207 4576
Fragments arranged by size:
4576 1338 1032 573 534 207 123 75
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3703 4993 5652 5908 6669 7697
Size: 275 186 1702 1290 659 256 761 1028
Cuts at: 7697 1540
Size: 2301
Fragments arranged by size:
2301 1702 1290 1028 761 659 275 256
186
BsiHKAI G_wGCw'C
Cuts at: 2423 3203 3579 3879 5477 5600 5667 6519 6604
Size: 780 376 300 1598 123 67 852 85
Cuts at: 6604 7675 2423
Size: 1071 3206
Fragments arranged by size:
3206 1598 1071 852 780 376 300 123
85 67
BsmI GAATG_Cn'
Cuts at: 5969 6062 5969
Size: 93 8365
BsmBI CGTCTCn'nnnn_
Cuts at: 3489 3489
Size: 8458
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2266 2423 2619 2709 3203 3315 3579
Size: 399 476 157 196 90 494 112 264
Cuts at: 3579 3797 3879 5318 5407 5477 5580 5600 5658
Size: 218 82 1439 89 70 103 20 58
Cuts at: 5658 5667 5690 5696 5777 6519 6604 7675 1391
Size: 9 23 6 81 742 85 1071 2174
Fragments arranged by size:
2174 1439 1071 742 494 476 399 264
218 196 157 112 103 90 89 85
82 81 70 58 23 20 9 6
BspEI T'CCGG_A
Cuts at: 5232 8121 5232
Size: 2889 5569
BspHI T'CATG_A
Cuts at: 2322 6347 8077 2322
Size: 4025 1730 2703
Fragments arranged by size:
4025 2703 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3145 3397 4236 4962 5017 5813 844
Size: 2301 252 839 726 55 796 3489
Fragments arranged by size:
3489 2301 839 796 726 252 55
BsrBI CCG'CTC
Cuts at: 1394 1823 3736 6345 1394
Size: 429 1913 2609 3507
Fragments arranged by size:
3507 2609 1913 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6953 7127 65
Size: 1183 5705 174 1396
Fragments arranged by size:
5705 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3702 4905 5124 5647 7102 7289 1700
Size: 110 1892 1203 219 523 1455 187 2869
Fragments arranged by size:
2869 1892 1455 1203 523 219 187 110
BsrGI T'GTAC_A
Cuts at: 96 4368 5001 96
Size: 4272 633 3553
Fragments arranged by size:
4272 3553 633
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8458
BssSI C'ACGA_G
Cuts at: 6518 7530 6518
Size: 1012 7446
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2815 4792 794
Size: 2021 1977 4460
Fragments arranged by size:
4460 2021 1977
BstBI TT'CG_AA
Cuts at: 7262 7262
Size: 8458
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8458
BstXI CCAn_nnnn'nTGG
Cuts at: 2770 5143 2770
Size: 2373 6085
BstYI r'GATC_y
Cuts at: 1972 2385 4728 5078 5676 5712 5788 6155 6540
Size: 413 2343 350 598 36 76 367 385
Cuts at: 6540 6557 8009 8095 8107 8453 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2343 1977 1452 598 413 385 367 350
346 86 76 36 17 12
Bsu36I CC'TnA_GG
Cuts at: 2133 2217 3345 2133
Size: 84 1128 7246
Fragments arranged by size:
7246 1128 84
BtgI C'CryG_G
Cuts at: 513 1065 3669 513
Size: 552 2604 5302
Fragments arranged by size:
5302 2604 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7719
Fragments arranged by size:
7719 683 56
BtsI GCAGTG_nn'
Cuts at: 5974 6768 6788 5974
Size: 794 20 7644
Fragments arranged by size:
7644 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8458
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8423
DraI TTT'AAA
Cuts at: 4305 5762 4305
Size: 1457 7001
DraIII CAC_nnn'GTG
Cuts at: 2418 5671 2418
Size: 3253 5205
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3773 7465 809
Size: 507 2457 3692 1802
Fragments arranged by size:
3692 2457 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3649 3672 4990
Size: 54 1425 50 275 18 1819 23 1318
Cuts at: 4990 5133 5421 5649 5905 6794 8423 8
Size: 143 288 228 256 889 1629 43
Fragments arranged by size:
1819 1629 1425 1318 889 288 275 256
228 143 54 50 43 23 18
EagI C'GGCC_G
Cuts at: 1537 1812 4990 5649 5905 1537
Size: 275 3178 659 256 4090
Fragments arranged by size:
4090 3178 659 275 256
EarI CTCTTCn'nnn_
Cuts at: 1353 2070 2166 3003 3051 3078 3084 3102 3252
Size: 717 96 837 48 27 6 18 150
Cuts at: 3252 3288 3444 3567 3731 3864 4925 5171 5279
Size: 36 156 123 164 133 1061 246 108
Cuts at: 5279 5333 5655 6388 1353
Size: 54 322 733 3423
Fragments arranged by size:
3423 1061 837 733 717 322 246 164
156 150 133 123 108 96 54 48
36 27 18 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2123 3341 4277 5494 5768 7045
Size: 1064 138 534 1218 936 1217 274 1277
Cuts at: 7045 7419 7565 387
Size: 374 146 1280
Fragments arranged by size:
1280 1277 1218 1217 1064 936 534 374
274 146 138
EcoICRI GAG'CTC
Cuts at: 3577 5475 5598 3577
Size: 1898 123 6437
Fragments arranged by size:
6437 1898 123
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2510 2563 2577 2685 2813 2913 3189
Size: 393 878 53 14 108 128 100 276
Cuts at: 3189 3276 3312 3591 3692 3819 3873 4223 4949
Size: 87 36 279 101 127 54 350 726
Cuts at: 4949 5450 6521 7118 7905 8173 1239
Size: 501 1071 597 787 268 1524
Fragments arranged by size:
1524 1071 878 787 726 597 501 393
350 279 276 268 128 127 108 101
100 87 54 53 36 14
EcoNI CCTnn'n_nnAGG
Cuts at: 2924 2924
Size: 8458
EcoO109I rG'GnC_Cy
Cuts at: 2046 2374 2440 2615 2755 2779 3186 4453 5185
Size: 328 66 175 140 24 407 1267 732
Cuts at: 5185 2046
Size: 5319
Fragments arranged by size:
5319 1267 732 407 328 175 140 66
24
EcoRI G'AATT_C
Cuts at: 4417 5767 6145 4417
Size: 1350 378 6730
Fragments arranged by size:
6730 1350 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4585 4585
Size: 8458
FseI GG_CCGG'CC
Cuts at: 5651 5651
Size: 8458
FspI TGC'GCA
Cuts at: 6964 6964
Size: 8458
HaeII r_GCGC'y
Cuts at: 1678 2274 2591 3690 7605 1678
Size: 596 317 1099 3915 2531
Fragments arranged by size:
3915 2531 1099 596 317
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2135 2141 2167 2173 3764
Size: 32 135 32 563 6 26 6 1591
Cuts at: 3764 3796 5036 5068 5697 5729 7099 7131 7173
Size: 32 1240 32 629 32 1370 32 42
Cuts at: 7173 7205 1373
Size: 32 2626
Fragments arranged by size:
2626 1591 1370 1240 629 563 135 42
32 32 32 32 32 32 32 26
6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 5802 6048 669
Size: 988 281 3864 246 3079
Fragments arranged by size:
3864 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 5098 748
Size: 4350 4108
HpaI GTT'AAC
Cuts at: 6048 6048
Size: 8458
KpnI G_GTAC'C
Cuts at: 5783 5783
Size: 8458
MfeI C'AATT_G
Cuts at: 6057 6057
Size: 8458
MluI A'CGCG_T
Cuts at: 8334 8334
Size: 8458
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7572 7756 1305
Size: 6267 184 2007
Fragments arranged by size:
6267 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3651 10
Size: 54 1425 343 1819 4817
Fragments arranged by size:
4817 1819 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2958 4124 4856 6416 6775 6934 518
Size: 648 1792 1166 732 1560 359 159 2042
Fragments arranged by size:
2042 1792 1560 1166 732 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3851 3955 4299 4588 4689 5874 6551 7699
Size: 2245 104 344 289 101 1185 677 1148
Cuts at: 7699 7944 1606
Size: 245 2120
Fragments arranged by size:
2245 2120 1185 1148 677 344 289 245
104 101
NaeI GCC'GGC
Cuts at: 5649 5649
Size: 8458
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7906
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8458
NgoMIV G'CCGG_C
Cuts at: 5647 5647
Size: 8458
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8458
NotI GC'GGCC_GC
Cuts at: 5905 5905
Size: 8458
NspI r_CATG'y
Cuts at: 1132 1229 3892 4189 4795 4927 5254 5816 7361
Size: 97 2663 297 606 132 327 562 1545
Cuts at: 7361 1132
Size: 2229
Fragments arranged by size:
2663 2229 1545 606 562 327 297 132
97
PasI CC'CwG_GG
Cuts at: 1348 2042 2266 2855 2940 1348
Size: 694 224 589 85 6866
Fragments arranged by size:
6866 694 589 224 85
PciI A'CATG_T
Cuts at: 7357 7357
Size: 8458
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2611 3715 4470 1108
Size: 1503 1104 755 5096
Fragments arranged by size:
5096 1503 1104 755
PfoI T'CCnGG_A
Cuts at: 2593 2890 3047 3708 4113 5172 2593
Size: 297 157 661 405 1059 5879
Fragments arranged by size:
5879 1059 661 405 297 157
PmeI GTTT'AAAC
Cuts at: 5762 5762
Size: 8458
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6527 6559 8040 8072 6527
Size: 32 1481 32 6913
Fragments arranged by size:
6913 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2046 2374 2755 2779 3186 5185 2046
Size: 328 381 24 407 1999 5319
Fragments arranged by size:
5319 1999 407 381 328 24
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8458
PsiI TTA'TAA
Cuts at: 6028 6028
Size: 8458
PspOMI G'GGCC_C
Cuts at: 2262 2615 5403 5692 2262
Size: 353 2788 289 5028
Fragments arranged by size:
5028 2788 353 289
PstI C_TGCA'G
Cuts at: 830 2560 2628 2670 4146 5810 830
Size: 1730 68 42 1476 1664 3478
Fragments arranged by size:
3478 1730 1664 1476 68 42
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8458
PvuII CAG'CTG
Cuts at: 4588 4689 4588
Size: 101 8357
SacI G_AGCT'C
Cuts at: 3579 5477 5600 3579
Size: 1898 123 6437
Fragments arranged by size:
6437 1898 123
SalI G'TCGA_C
Cuts at: 1936 5800 1936
Size: 3864 4594
SanDI GG'GwC_CC
Cuts at: 2046 2755 3186 2046
Size: 709 431 7318
Fragments arranged by size:
7318 709 431
SapI GCTCTTCn'nnn_
Cuts at: 3567 3731 5279 5333 5655 3567
Size: 164 1548 54 322 6370
Fragments arranged by size:
6370 1548 322 164 54
SbfI CC_TGCA'GG
Cuts at: 5810 5810
Size: 8458
ScaI AGT'ACT
Cuts at: 6706 8418 6706
Size: 1712 6746
SexAI A'CCwGG_T
Cuts at: 4760 4760
Size: 8458
SfcI C'TryA_G
Cuts at: 826 1045 2556 2565 2624 2666 4142 4402 5806
Size: 219 1511 9 59 42 1476 260 1404
Cuts at: 5806 6941 7622 7813 826
Size: 1135 681 191 1471
Fragments arranged by size:
1511 1476 1471 1404 1135 681 260 219
191 59 42 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 2447 3671 2447
Size: 1224 7234
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8458
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8458
SmaI CCC'GGG
Cuts at: 1872 2633 5309 5583 5691 5785 1872
Size: 761 2676 274 108 94 4545
Fragments arranged by size:
4545 2676 761 274 108 94
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2444 3578 6562 7463 7725 820
Size: 197 926 501 1134 2984 901 262 1553
Fragments arranged by size:
2984 1553 1134 926 901 501 262 197
SnaBI TAC'GTA
Cuts at: 493 6249 493
Size: 5756 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8458
SphI G_CATG'C
Cuts at: 1132 4795 5254 5816 1132
Size: 3663 459 562 3774
Fragments arranged by size:
3774 3663 562 459
SrfI GCCC'GGGC
Cuts at: 5691 5691
Size: 8458
SspI AAT'ATT
Cuts at: 5 52 6382 5
Size: 47 6330 2081
Fragments arranged by size:
6330 2081 47
StuI AGG'CCT
Cuts at: 1517 2464 1517
Size: 947 7511
StyI C'CwwG_G
Cuts at: 513 1065 2226 2673 2685 3267 3333 4245 5274
Size: 552 1161 447 12 582 66 912 1029
Cuts at: 5274 5617 5897 513
Size: 343 280 3074
Fragments arranged by size:
3074 1161 1029 912 582 552 447 343
280 66 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2671 3411 5351 5544 6485 6502 6655
Size: 1255 1091 740 1940 193 941 17 153
Cuts at: 6655 6840 7285 325
Size: 185 445 1498
Fragments arranged by size:
1940 1498 1255 1091 941 740 445 193
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4308 4368 4710
Size: 274 80 33 51 1443 2331 60 342
Cuts at: 4710 4770 5001 6704 8416 96
Size: 60 231 1703 1712 138
Fragments arranged by size:
2331 1712 1703 1443 342 274 231 138
80 60 60 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2402 2434 2886 2918 8418 8450 2402
Size: 32 452 32 5500 32 2410
Fragments arranged by size:
5500 2410 452 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2149 2915 1316
Size: 833 766 6859
Fragments arranged by size:
6859 833 766
XbaI T'CTAG_A
Cuts at: 5794 5794
Size: 8458
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3612 3612
Size: 8458
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8458
XmaI C'CCGG_G
Cuts at: 1870 2631 5307 5581 5689 5783 1870
Size: 761 2676 274 108 94 4545
Fragments arranged by size:
4545 2676 761 274 108 94
XmnI GAAnn'nnTTC
Cuts at: 3725 4598 6587 3725
Size: 873 1989 5596
Fragments arranged by size:
5596 1989 873
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3775 5485 6266 276
Size: 53 83 186 936 2241 1710 781 2468
Fragments arranged by size:
2468 2241 1710 936 781 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BfrBI BsiWI BstZ17I
EcoRV FspAI KasI NarI NruI NsiI PacI PmlI
PspXI PsrI RsrII SacII SfoI SwaI