Restriction map for
pFN21AB7300
(Circular) (Six-base) MAPSORT of: pFN21AB7300.seq Check: 787 from: 1 to: 7573
pFN21AB7300 7573 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4426 4758 4426
Size: 332 7241
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5383 278
Size: 53 83 186 936 3847 2468
Fragments arranged by size:
3847 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3837 4916 1937
Size: 1900 1079 4594
Fragments arranged by size:
4594 1900 1079
Acc65I G'GTAC_C
Cuts at: 3515 4745 4894 3515
Size: 1230 149 6194
Fragments arranged by size:
6194 1230 149
AclI AA'CG_TT
Cuts at: 1563 5700 6073 1563
Size: 4137 373 3063
Fragments arranged by size:
4137 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2148 2555 3981 4240 5636 7020 7288 1632
Size: 516 407 1426 259 1396 1384 268 1917
Fragments arranged by size:
1917 1426 1396 1384 516 407 268 259
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7376
AflIII A'CryG_T
Cuts at: 1203 2808 6472 7449 1203
Size: 1605 3664 977 1327
Fragments arranged by size:
3664 1605 1327 977
AgeI A'CCGG_T
Cuts at: 6404 6404
Size: 7573
AhdI GACnn_n'nnGTC
Cuts at: 6302 6302
Size: 7573
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7573
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2283 3103 4558 1184
Size: 1099 820 1455 4199
Fragments arranged by size:
4199 1455 1099 820
AlwNI CAG_nnn'CTG
Cuts at: 2562 3589 5316 6888 2562
Size: 1027 1727 1572 3247
Fragments arranged by size:
3247 1727 1572 1027
ApaI G_GGCC'C
Cuts at: 4039 4470 4039
Size: 431 7142
ApaLI G'TGCA_C
Cuts at: 5630 6786 5630
Size: 1156 6417
ApoI r'AATT_y
Cuts at: 1493 4882 5108 5260 7369 1493
Size: 3389 226 152 2109 1697
Fragments arranged by size:
3389 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2906 6127 160
Size: 872 1874 3221 1606
Fragments arranged by size:
3221 1874 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7573
AvaI C'yCGr_G
Cuts at: 1870 1943 2518 4469 4898 1870
Size: 73 575 1951 429 4545
Fragments arranged by size:
4545 1951 575 429 73
BamHI G'GATC_C
Cuts at: 4903 5270 4903
Size: 367 7206
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2021 2930 3138 3515 3775
Size: 325 217 627 234 909 208 377 260
Cuts at: 3775 3827 4745 4864 4894 6349 618
Size: 52 918 119 30 1455 1842
Fragments arranged by size:
1842 1455 918 909 627 377 325 260
234 217 208 119 52 30
BanII G_rGCy'C
Cuts at: 1391 2208 2460 2569 3607 3746 4039 4470 4758
Size: 817 252 109 1038 139 293 431 288
Cuts at: 4758 4892 1391
Size: 134 4072
Fragments arranged by size:
4072 1038 817 431 293 288 252 139
134 109
BbeI G_GCGC'C
Cuts at: 2025 2025
Size: 7573
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2165 928
Size: 971 266 6336
Fragments arranged by size:
6336 971 266
BbvCI CC'TCA_GC
Cuts at: 2220 2441 4213 4232 4674 2220
Size: 221 1772 19 442 5119
Fragments arranged by size:
5119 1772 442 221 19
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2920 2954 5764 5798 1903
Size: 34 50 34 899 34 2810 34 3678
Fragments arranged by size:
3678 2810 899 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5465 6675 5465
Size: 1210 6363
BclI T'GATC_A
Cuts at: 1548 4335 1548
Size: 2787 4786
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2065 3035 3101
Size: 107 122 71 631 605 393 970 66
Cuts at: 3101 4029 4068 6184 136
Size: 928 39 2116 1525
Fragments arranged by size:
2116 1525 970 928 631 605 393 122
107 71 66 39
BglII A'GATC_T
Cuts at: 2495 7568 2495
Size: 5073 2500
BlpI GC'TnA_GC
Cuts at: 2202 2393 4990 2202
Size: 191 2597 4785
Fragments arranged by size:
4785 2597 191
Bme1580I G_kGCm'C
Cuts at: 1790 3141 3780 3830 4039 4470 4869 5634 6790
Size: 1351 639 50 209 431 399 765 1156
Cuts at: 6790 1790
Size: 2573
Fragments arranged by size:
2573 1351 1156 765 639 431 399 209
50
BmgBI CAC'GTC
Cuts at: 1318 3835 1318
Size: 2517 5056
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2572 3144 4598
Size: 416 53 83 399 24 1155 572 1454
Cuts at: 4598 5304 6262 442
Size: 706 958 1753
Fragments arranged by size:
1753 1454 1155 958 706 572 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7573
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4527 4527
Size: 7573
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2267 3003 3782 4143 4374 4549 4742 6233
Size: 1028 736 779 361 231 175 193 1491
Cuts at: 6233 1239
Size: 2579
Fragments arranged by size:
2579 1491 1028 779 736 361 231 193
175
Bpu10I CC'TnA_GC
Cuts at: 2207 2220 2441 4213 4232 4439 4674 2207
Size: 13 221 1772 19 207 235 5106
Fragments arranged by size:
5106 1772 235 221 207 19 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2492 4190 4715 5698 6563 6861 2492
Size: 1698 525 983 865 298 3204
Fragments arranged by size:
3204 1698 983 865 525 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2685 4479 6236 882
Size: 617 1186 1794 1757 2219
Fragments arranged by size:
2219 1794 1757 1186 617
BsaAI yAC'GTr
Cuts at: 493 1204 5364 493
Size: 711 4160 2702
Fragments arranged by size:
4160 2702 711
BsaBI GATnn'nnATC
Cuts at: 7567 7567
Size: 7573
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2022 2700 4313 5380
Size: 53 83 186 936 489 678 1613 1067
Cuts at: 5380 5762 6397 275
Size: 382 635 1451
Fragments arranged by size:
1613 1451 1067 936 678 635 489 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2766 4345 6006 6404 6678 6825 7236 2766
Size: 1579 1661 398 274 147 411 3103
Fragments arranged by size:
3103 1661 1579 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4116 4146 1178
Size: 30 165 30 2713 30 4605
Fragments arranged by size:
4605 2713 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3000 3866 3869 4365 4500 1186
Size: 1814 866 3 496 135 4259
Fragments arranged by size:
4259 1814 866 496 135 3
BseYI C'CCAG_C
Cuts at: 4032 4368 6776 7272 4032
Size: 336 2408 496 4333
Fragments arranged by size:
4333 2408 496 336
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3955 4404 1163
Size: 573 2219 449 4332
Fragments arranged by size:
4332 2219 573 449
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2069 4577 5023 5784 6812 1540
Size: 275 186 68 2508 446 761 1028 2301
Fragments arranged by size:
2508 2301 1028 761 446 275 186 68
BsiHKAI G_wGCw'C
Cuts at: 2208 2541 2969 3379 3746 5634 5719 6790 2208
Size: 333 428 410 367 1888 85 1071 2991
Fragments arranged by size:
2991 1888 1071 428 410 367 333 85
BsmI GAATG_Cn'
Cuts at: 3016 5084 5177 3016
Size: 2068 93 5412
Fragments arranged by size:
5412 2068 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2208 2460 2541 2569 2969 3141 3379
Size: 399 418 252 81 28 400 172 238
Cuts at: 3379 3607 3746 3780 3830 4039 4470 4758 4869
Size: 228 139 34 50 209 431 288 111
Cuts at: 4869 4892 5634 5719 6790 1391
Size: 23 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 431 418 400 399 288
252 238 228 209 172 139 111 85
81 50 34 28 23
BspEI T'CCGG_A
Cuts at: 2766 4345 7236 2766
Size: 1579 2891 3103
Fragments arranged by size:
3103 2891 1579
BspHI T'CATG_A
Cuts at: 3798 5462 7192 3798
Size: 1664 1730 4179
Fragments arranged by size:
4179 1730 1664
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3619 4426 4758 4928 844
Size: 2775 807 332 170 3489
Fragments arranged by size:
3489 2775 807 332 170
BsrBI CCG'CTC
Cuts at: 1394 1823 5460 1394
Size: 429 3637 3507
Fragments arranged by size:
3637 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3343 3988 6068 6242 65
Size: 1183 2095 645 2080 174 1396
Fragments arranged by size:
2095 2080 1396 1183 645 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4518 6217 6404 1700
Size: 110 2708 1699 187 2869
Fragments arranged by size:
2869 2708 1699 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7573
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7573
BssSI C'ACGA_G
Cuts at: 2654 2777 3041 5633 6645 2654
Size: 123 264 2592 1012 3582
Fragments arranged by size:
3582 2592 1012 264 123
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4700 4798 794
Size: 3906 98 3569
Fragments arranged by size:
3906 3569 98
BstBI TT'CG_AA
Cuts at: 6377 6377
Size: 7573
BstEII G'GTnAC_C
Cuts at: 1184 2306 1184
Size: 1122 6451
BstXI CCAn_nnnn'nTGG
Cuts at: 2944 3022 3059 3512 2944
Size: 78 37 453 7005
Fragments arranged by size:
7005 453 78 37
BstYI r'GATC_y
Cuts at: 1972 2495 3729 3813 4903 5270 5655 5672 7124
Size: 523 1234 84 1090 367 385 17 1452
Cuts at: 7124 7210 7222 7568 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1234 1090 523 385 367 346
86 84 17 12
Bsu36I CC'TnA_GG
Cuts at: 3356 3849 3356
Size: 493 7080
BtgI C'CryG_G
Cuts at: 513 1065 2069 2353 2800 2926 3033 3988 4063
Size: 552 1004 284 447 126 107 955 75
Cuts at: 4063 4718 513
Size: 655 3368
Fragments arranged by size:
3368 1004 955 655 552 447 284 126
107 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6834
Fragments arranged by size:
6834 683 56
BtsI GCAGTG_nn'
Cuts at: 2341 3452 3484 3584 3938 4018 4424 4649 5089
Size: 1111 32 100 354 80 406 225 440
Cuts at: 5089 5883 5903 2341
Size: 794 20 4011
Fragments arranged by size:
4011 1111 794 440 406 354 225 100
80 32 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7573
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7538
DraI TTT'AAA
Cuts at: 2625 4877 2625
Size: 2252 5321
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2377 4495 6580 809
Size: 507 1061 2118 2085 1802
Fragments arranged by size:
2118 2085 1802 1061 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2066 2958 3093
Size: 54 1425 50 275 18 236 892 135
Cuts at: 3093 3532 3985 4134 4520 4574 5020 5909 7538
Size: 439 453 149 386 54 446 889 1629
Cuts at: 7538 8
Size: 43
Fragments arranged by size:
1629 1425 892 889 453 446 439 386
275 236 149 135 54 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2066 4574 5020 1537
Size: 275 254 2508 446 4090
Fragments arranged by size:
4090 2508 446 275 254
EarI CTCTTCn'nnn_
Cuts at: 1353 2574 3285 3306 5503 1353
Size: 1221 711 21 2197 3423
Fragments arranged by size:
3423 2197 1221 711 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3383 6160 6534 6680 387
Size: 1064 138 1794 2777 374 146 1280
Fragments arranged by size:
2777 1794 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2206 3744 2206
Size: 1538 6035
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2148 2267 2555 3003 3782 3981 4143
Size: 393 516 119 288 448 779 199 162
Cuts at: 4143 4240 4374 4549 4742 5636 6233 7020 7288
Size: 97 134 175 193 894 597 787 268
Cuts at: 7288 1239
Size: 1524
Fragments arranged by size:
1524 894 787 779 597 516 448 393
288 268 199 193 175 162 134 119
97
EcoO109I rG'GnC_Cy
Cuts at: 3420 3596 3845 4036 4109 4410 4466 3420
Size: 176 249 191 73 301 56 6527
Fragments arranged by size:
6527 301 249 191 176 73 56
EcoRI G'AATT_C
Cuts at: 4882 5260 4882
Size: 378 7195
FspI TGC'GCA
Cuts at: 6079 6079
Size: 7573
HaeII r_GCGC'y
Cuts at: 1678 2025 6720 1678
Size: 347 4695 2531
Fragments arranged by size:
4695 2531 347
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2220 2252 2612 2644 3074
Size: 32 135 32 648 32 360 32 430
Cuts at: 3074 3106 3721 3753 4093 4125 4327 4359 6214
Size: 32 615 32 340 32 202 32 1855
Cuts at: 6214 6246 6288 6320 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1855 648 615 430 360 340 202
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2992 4917 5163 669
Size: 988 281 1054 1925 246 3079
Fragments arranged by size:
3079 1925 1054 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7573
HpaI GTT'AAC
Cuts at: 5163 5163
Size: 7573
KasI G'GCGC_C
Cuts at: 2021 2021
Size: 7573
KpnI G_GTAC'C
Cuts at: 3519 4749 4898 3519
Size: 1230 149 6194
Fragments arranged by size:
6194 1230 149
MfeI C'AATT_G
Cuts at: 5172 5172
Size: 7573
MluI A'CGCG_T
Cuts at: 7449 7449
Size: 7573
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2840 3246 3792 6687 6871 1305
Size: 1535 406 546 2895 184 2007
Fragments arranged by size:
2895 2007 1535 546 406 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2960 3987 4136 10
Size: 54 1425 343 1128 1027 149 3447
Fragments arranged by size:
3447 1425 1128 1027 343 149 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2915 2960 3020 3239 4142 4163 5531
Size: 648 1749 45 60 219 903 21 1368
Cuts at: 5531 5890 6049 518
Size: 359 159 2042
Fragments arranged by size:
2042 1749 1368 903 648 359 219 159
60 45 21
MspA1I CmG'CkG
Cuts at: 1606 2071 3128 3589 4275 4871 4989 5666 6814
Size: 465 1057 461 686 596 118 677 1148
Cuts at: 6814 7059 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1057 686 677 596 465 461
245 118
NaeI GCC'GGC
Cuts at: 4520 4520
Size: 7573
NarI GG'CG_CC
Cuts at: 2022 2022
Size: 7573
NcoI C'CATG_G
Cuts at: 513 1065 3033 3988 513
Size: 552 1968 955 4098
Fragments arranged by size:
4098 1968 955 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7573
NgoMIV G'CCGG_C
Cuts at: 4518 4518
Size: 7573
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7573
NotI GC'GGCC_GC
Cuts at: 5020 5020
Size: 7573
NspI r_CATG'y
Cuts at: 1132 1229 2812 2920 3229 3559 3868 4931 6476
Size: 97 1583 108 309 330 309 1063 1545
Cuts at: 6476 1132
Size: 2229
Fragments arranged by size:
2229 1583 1545 1063 330 309 309 108
97
PasI CC'CwG_GG
Cuts at: 1348 3875 4318 4463 1348
Size: 2527 443 145 4458
Fragments arranged by size:
4458 2527 443 145
PciI A'CATG_T
Cuts at: 2808 6472 2808
Size: 3664 3909
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2562 3046 1108
Size: 1454 484 5635
Fragments arranged by size:
5635 1454 484
PfoI T'CCnGG_A
Cuts at: 2238 3456 4499 2238
Size: 1218 1043 5312
Fragments arranged by size:
5312 1218 1043
PmeI GTTT'AAAC
Cuts at: 4877 4877
Size: 7573
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5642 5674 7155 7187 5642
Size: 32 1481 32 6028
Fragments arranged by size:
6028 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3420 3596 3845 4109 4410 3420
Size: 176 249 264 301 6583
Fragments arranged by size:
6583 301 264 249 176
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7573
PsiI TTA'TAA
Cuts at: 5143 5143
Size: 7573
PspOMI G'GGCC_C
Cuts at: 4035 4466 4035
Size: 431 7142
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2130 2162 2130
Size: 32 7541
PstI C_TGCA'G
Cuts at: 830 2058 2133 3082 3198 4181 4311 4925 830
Size: 1228 75 949 116 983 130 614 3478
Fragments arranged by size:
3478 1228 983 949 614 130 116 75
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7573
PvuII CAG'CTG
Cuts at: 3128 3589 3128
Size: 461 7112
SacI G_AGCT'C
Cuts at: 2208 3746 2208
Size: 1538 6035
SacII CC_GC'GG
Cuts at: 2072 2072
Size: 7573
SalI G'TCGA_C
Cuts at: 1936 4915 1936
Size: 2979 4594
SanDI GG'GwC_CC
Cuts at: 3420 3596 4410 3420
Size: 176 814 6583
Fragments arranged by size:
6583 814 176
SapI GCTCTTCn'nnn_
Cuts at: 2574 2574
Size: 7573
SbfI CC_TGCA'GG
Cuts at: 4925 4925
Size: 7573
ScaI AGT'ACT
Cuts at: 5821 7533 5821
Size: 1712 5861
SfcI C'TryA_G
Cuts at: 826 1045 2054 2129 3078 3194 4177 4307 4921
Size: 219 1009 75 949 116 983 130 614
Cuts at: 4921 6056 6737 6928 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1009 983 949 681 614 219
191 130 116 75
SfoI GGC'GCC
Cuts at: 2023 2023
Size: 7573
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7573
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7573
SmaI CCC'GGG
Cuts at: 1872 2520 4471 4900 1872
Size: 648 1951 429 4545
Fragments arranged by size:
4545 1951 648 429
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2507 4205 4730 5677 6578 6840
Size: 197 926 564 1698 525 947 901 262
Cuts at: 6840 820
Size: 1553
Fragments arranged by size:
1698 1553 947 926 901 564 525 262
197
SnaBI TAC'GTA
Cuts at: 493 5364 493
Size: 4871 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7573
SphI G_CATG'C
Cuts at: 1132 4931 1132
Size: 3799 3774
SspI AAT'ATT
Cuts at: 5 52 5497 5
Size: 47 5445 2081
Fragments arranged by size:
5445 2081 47
StuI AGG'CCT
Cuts at: 1517 3539 4507 4570 1517
Size: 2022 968 63 4520
Fragments arranged by size:
4520 2022 968 63
StyI C'CwwG_G
Cuts at: 513 1065 3033 3988 4838 5012 513
Size: 552 1968 955 850 174 3074
Fragments arranged by size:
3074 1968 955 850 552 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3102 3845 5600 5617 5770 5955 6400
Size: 1255 1522 743 1755 17 153 185 445
Cuts at: 6400 325
Size: 1498
Fragments arranged by size:
1755 1522 1498 1255 743 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4167 5819 7531
Size: 274 80 33 51 1443 2190 1652 1712
Cuts at: 7531 96
Size: 138
Fragments arranged by size:
2190 1712 1652 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2797 2829 4116 4148 7533 7565 2797
Size: 32 1287 32 3385 32 2805
Fragments arranged by size:
3385 2805 1287 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4492 1316
Size: 3176 4397
XbaI T'CTAG_A
Cuts at: 4909 4909
Size: 7573
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2329 2638 2941 4144 2329
Size: 309 303 1203 5758
Fragments arranged by size:
5758 1203 309 303
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7573
XmaI C'CCGG_G
Cuts at: 1870 2518 4469 4898 1870
Size: 648 1951 429 4545
Fragments arranged by size:
4545 1951 648 429
XmnI GAAnn'nnTTC
Cuts at: 5702 5702
Size: 7573
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5381 276
Size: 53 83 186 936 3847 2468
Fragments arranged by size:
3847 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NruI
NsiI PacI PmlI PspXI RsrII SexAI SfiI SrfI
SwaI