Restriction map for
pFN21AB7299
(Circular) (Six-base) MAPSORT of: pFN21AB7299.seq Check: 9299 from: 1 to: 8377
pFN21AB7299 8377 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2053 2089 4975 2053
Size: 36 2886 5455
Fragments arranged by size:
5455 2886 36
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4206 6187 278
Size: 53 83 186 936 2670 1981 2468
Fragments arranged by size:
2670 2468 1981 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2898 5720 1937
Size: 961 2822 4594
Fragments arranged by size:
4594 2822 961
Acc65I G'GTAC_C
Cuts at: 5602 5698 5602
Size: 96 8281
AclI AA'CG_TT
Cuts at: 1563 6504 6877 1563
Size: 4941 373 3063
Fragments arranged by size:
4941 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3920 4724 5120 6440 7824 8092 1632
Size: 2288 804 396 1320 1384 268 1917
Fragments arranged by size:
2288 1917 1384 1320 804 396 268
AfeI AGC'GCT
Cuts at: 2840 5141 2840
Size: 2301 6076
AflII C'TTAA_G
Cuts at: 820 1017 3088 820
Size: 197 2071 6109
Fragments arranged by size:
6109 2071 197
AflIII A'CryG_T
Cuts at: 1203 2226 3348 3702 4829 5201 7276 8253 1203
Size: 1023 1122 354 1127 372 2075 977 1327
Fragments arranged by size:
2075 1327 1127 1122 1023 977 372 354
AgeI A'CCGG_T
Cuts at: 7208 7208
Size: 8377
AhdI GACnn_n'nnGTC
Cuts at: 7106 7106
Size: 8377
AleI CACnn'nnGTG
Cuts at: 1166 2083 2945 5007 1166
Size: 917 862 2062 4536
Fragments arranged by size:
4536 2062 917 862
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2097 2101 4620 1184
Size: 913 4 2519 4941
Fragments arranged by size:
4941 2519 913 4
AlwNI CAG_nnn'CTG
Cuts at: 2114 3807 5077 5170 5464 6120 7692 2114
Size: 1693 1270 93 294 656 1572 2799
Fragments arranged by size:
2799 1693 1572 1270 656 294 93
ApaI G_GGCC'C
Cuts at: 4986 4986
Size: 8377
ApaLI G'TGCA_C
Cuts at: 4864 4877 5416 6434 7590 4864
Size: 13 539 1018 1156 5651
Fragments arranged by size:
5651 1156 1018 539 13
ApoI r'AATT_y
Cuts at: 1493 3446 3662 4433 5686 5912 6064 8173 1493
Size: 1953 216 771 1253 226 152 2109 1697
Fragments arranged by size:
2109 1953 1697 1253 771 226 216 152
AseI AT'TA_AT
Cuts at: 160 1032 3261 6931 160
Size: 872 2229 3670 1606
Fragments arranged by size:
3670 2229 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8377
AvaI C'yCGr_G
Cuts at: 1870 1943 2122 3927 4478 4549 5380 5702 1870
Size: 73 179 1805 551 71 831 322 4545
Fragments arranged by size:
4545 1805 831 551 322 179 73 71
BamHI G'GATC_C
Cuts at: 5707 6074 5707
Size: 367 8010
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2077 3630 3708 4762 4841
Size: 325 217 627 290 1553 78 1054 79
Cuts at: 4841 5352 5602 5698 7153 618
Size: 511 250 96 1455 1842
Fragments arranged by size:
1842 1553 1455 1054 627 511 325 290
250 217 96 79 78
BanII G_rGCy'C
Cuts at: 1391 2123 2637 3198 3887 4318 4479 4986 5106
Size: 732 514 561 689 431 161 507 120
Cuts at: 5106 5696 1391
Size: 590 4072
Fragments arranged by size:
4072 732 689 590 561 514 507 431
161 120
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2761 2796 3400 3774 4206 928
Size: 971 862 35 604 374 432 5099
Fragments arranged by size:
5099 971 862 604 432 374 35
BbvCI CC'TCA_GC
Cuts at: 4917 4917
Size: 8377
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4432 4466 5437 5471 6568 6602 1903
Size: 34 2495 34 971 34 1097 34 3678
Fragments arranged by size:
3678 2495 1097 971 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3522 4334 6269 7479 3522
Size: 812 1935 1210 4420
Fragments arranged by size:
4420 1935 1210 812
BclI T'GATC_A
Cuts at: 1548 3481 1548
Size: 1933 6444
BfrBI ATG'CAT
Cuts at: 5119 5119
Size: 8377
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2075 5522 6988
Size: 107 122 71 631 605 403 3447 1466
Cuts at: 6988 136
Size: 1525
Fragments arranged by size:
3447 1525 1466 631 605 403 122 107
71
BglII A'GATC_T
Cuts at: 8372 8372
Size: 8377
BlpI GC'TnA_GC
Cuts at: 5794 5794
Size: 8377
Bme1580I G_kGCm'C
Cuts at: 1790 2274 4404 4445 4846 4868 4881 4916 4986
Size: 484 2130 41 401 22 13 35 70
Cuts at: 4986 5420 6438 7594 1790
Size: 434 1018 1156 2573
Fragments arranged by size:
2573 2130 1156 1018 484 434 401 70
41 35 22 13
BmgBI CAC'GTC
Cuts at: 1318 4830 1318
Size: 3512 4865
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5182 6108 7066
Size: 416 53 83 399 24 3765 926 958
Cuts at: 7066 442
Size: 1753
Fragments arranged by size:
3765 1753 958 926 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8377
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5293 5293
Size: 8377
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2299 3784 4332 4831 4915 5333 7037 1239
Size: 1060 1485 548 499 84 418 1704 2579
Fragments arranged by size:
2579 1704 1485 1060 548 499 418 84
Bpu10I CC'TnA_GC
Cuts at: 4917 4917
Size: 8377
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2656 3447 4002 6502 7367 7665 2656
Size: 791 555 2500 865 298 3368
Fragments arranged by size:
3368 2500 865 791 555 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2197 2876 4259 4692 5415 7040 882
Size: 617 698 679 1383 433 723 1625 2219
Fragments arranged by size:
2219 1625 1383 723 698 679 617 433
BsaAI yAC'GTr
Cuts at: 493 1204 2227 2611 2947 6168 493
Size: 711 1023 384 336 3221 2702
Fragments arranged by size:
3221 2702 1023 711 384 336
BsaBI GATnn'nnATC
Cuts at: 8371 8371
Size: 8377
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4203 6184 6566 7201
Size: 53 83 186 936 2670 1981 382 635
Cuts at: 7201 275
Size: 1451
Fragments arranged by size:
2670 1981 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6810 7208 7482 7629 8040 6810
Size: 398 274 147 411 7147
Fragments arranged by size:
7147 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2456 2486 3804 3834 4517
Size: 30 165 30 1053 30 1318 30 683
Cuts at: 4517 4547 1178
Size: 30 5008
Fragments arranged by size:
5008 1318 1053 683 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2130 2464 1186
Size: 944 334 7099
Fragments arranged by size:
7099 944 334
BseYI C'CCAG_C
Cuts at: 2775 4608 4613 4790 5306 5468 5607 7580 8076
Size: 1833 5 177 516 162 139 1973 496
Cuts at: 8076 2775
Size: 3076
Fragments arranged by size:
3076 1973 1833 516 496 177 162 139
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2399 2432 2687 3238 3826 4222 4880
Size: 573 663 33 255 551 588 396 658
Cuts at: 4880 5077 5477 5483 5641 1163
Size: 197 400 6 158 3899
Fragments arranged by size:
3899 663 658 588 573 551 400 396
255 197 158 33 6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2159 4426 5480 5827 6588 7616
Size: 275 186 158 2267 1054 347 761 1028
Cuts at: 7616 1540
Size: 2301
Fragments arranged by size:
2301 2267 1054 1028 761 347 275 186
158
BsiHKAI G_wGCw'C
Cuts at: 2952 3969 4649 4868 4881 5000 5051 5195 5291
Size: 1017 680 219 13 119 51 144 96
Cuts at: 5291 5420 6438 6523 7594 2952
Size: 129 1018 85 1071 3735
Fragments arranged by size:
3735 1071 1018 1017 680 219 144 129
119 96 85 51 13
BsmI GAATG_Cn'
Cuts at: 5888 5981 5888
Size: 93 8284
BsmBI CGTCTCn'nnnn_
Cuts at: 4351 4351
Size: 8377
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2123 2274 2637 2952 3198 3887 3969
Size: 399 333 151 363 315 246 689 82
Cuts at: 3969 4318 4404 4445 4479 4649 4846 4868 4881
Size: 349 86 41 34 170 197 22 13
Cuts at: 4881 4916 4986 5000 5051 5106 5195 5291 5420
Size: 35 70 14 51 55 89 96 129
Cuts at: 5420 5696 6438 6523 7594 1391
Size: 276 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 689 399 363 349 333
315 276 246 197 170 151 129 96
89 86 85 82 70 55 51 41
35 34 22 14 13
BspEI T'CCGG_A
Cuts at: 8040 8040
Size: 8377
BspHI T'CATG_A
Cuts at: 3142 3331 6266 7996 3142
Size: 189 2935 1730 3523
Fragments arranged by size:
3523 2935 1730 189
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2053 2089 2501 3815 4975 5732 844
Size: 1209 36 412 1314 1160 757 3489
Fragments arranged by size:
3489 1314 1209 1160 757 412 36
BsrBI CCG'CTC
Cuts at: 1394 1823 2155 3461 6264 1394
Size: 429 332 1306 2803 3507
Fragments arranged by size:
3507 2803 1306 429 332
BsrDI GCAATG_nn'
Cuts at: 65 1248 3012 3294 3406 4378 5547 6872 7046
Size: 1183 1764 282 112 972 1169 1325 174
Cuts at: 7046 65
Size: 1396
Fragments arranged by size:
1764 1396 1325 1183 1169 972 282 174
112
BsrFI r'CCGG_y
Cuts at: 1700 1810 4425 5475 5479 7021 7208 1700
Size: 110 2615 1050 4 1542 187 2869
Fragments arranged by size:
2869 2615 1542 1050 187 110 4
BsrGI T'GTAC_A
Cuts at: 96 2616 96
Size: 2520 5857
BssHII G'CGCG_C
Cuts at: 1925 2016 2018 1925
Size: 91 2 8284
Fragments arranged by size:
8284 91 2
BssSI C'ACGA_G
Cuts at: 6437 7449 6437
Size: 1012 7365
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5077 5646 5658 794
Size: 4283 569 12 3513
Fragments arranged by size:
4283 3513 569 12
BstBI TT'CG_AA
Cuts at: 7181 7181
Size: 8377
BstEII G'GTnAC_C
Cuts at: 1184 2665 3417 4297 1184
Size: 1481 752 880 5264
Fragments arranged by size:
5264 1481 880 752
BstXI CCAn_nnnn'nTGG
Cuts at: 2645 3308 3641 4971 2645
Size: 663 333 1330 6051
Fragments arranged by size:
6051 1330 663 333
BstYI r'GATC_y
Cuts at: 1972 2335 2624 4184 5707 6074 6459 6476 7928
Size: 363 289 1560 1523 367 385 17 1452
Cuts at: 7928 8014 8026 8372 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1560 1523 1452 385 367 363 346
289 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2543 5485 2543
Size: 2942 5435
BtgI C'CryG_G
Cuts at: 513 1065 2091 2148 2920 3760 4410 4800 513
Size: 552 1026 57 772 840 650 390 4090
Fragments arranged by size:
4090 1026 840 772 650 552 390 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3843 487
Size: 683 56 2617 5021
Fragments arranged by size:
5021 2617 683 56
BtsI GCAGTG_nn'
Cuts at: 2274 4868 5040 5171 5465 5651 5893 6687 6707
Size: 2594 172 131 294 186 242 794 20
Cuts at: 6707 2274
Size: 3944
Fragments arranged by size:
3944 2594 794 294 242 186 172 131
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8377
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8342
DraI TTT'AAA
Cuts at: 3604 5681 3604
Size: 2077 6300
DraIII CAC_nnn'GTG
Cuts at: 3555 3555
Size: 8377
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7384 809
Size: 507 6068 1802
Fragments arranged by size:
6068 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2067 2156 2923
Size: 54 1425 50 275 18 237 89 767
Cuts at: 2923 3763 4423 4902 5061 5421 5477 5824 6713
Size: 840 660 479 159 360 56 347 889
Cuts at: 6713 8342 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 840 767 660 479 360
347 275 237 159 89 56 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2156 4423 5477 5824 1537
Size: 275 344 2267 1054 347 4090
Fragments arranged by size:
4090 2267 1054 347 344 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2257 2298 2520 2801 2850 6307 1353
Size: 904 41 222 281 49 3457 3423
Fragments arranged by size:
3457 3423 904 281 222 49 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2272 5167 6964 7338 7484 387
Size: 1064 138 683 2895 1797 374 146 1280
Fragments arranged by size:
2895 1797 1280 1064 683 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2299 3784 3920 4332 4724 4831 4915
Size: 393 667 1485 136 412 392 107 84
Cuts at: 4915 5120 5333 6440 7037 7824 8092 1239
Size: 205 213 1107 597 787 268 1524
Fragments arranged by size:
1524 1485 1107 787 667 597 412 393
392 268 213 205 136 107 84
EcoNI CCTnn'n_nnAGG
Cuts at: 4933 4933
Size: 8377
EcoO109I rG'GnC_Cy
Cuts at: 2096 2659 2853 3746 3804 4983 5375 2096
Size: 563 194 893 58 1179 392 5098
Fragments arranged by size:
5098 1179 893 563 392 194 58
EcoRI G'AATT_C
Cuts at: 5686 6064 5686
Size: 378 7999
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2842 5689 2842
Size: 2847 5530
FseI GG_CCGG'CC
Cuts at: 5479 5483 5479
Size: 4 8373
FspI TGC'GCA
Cuts at: 4370 6883 4370
Size: 2513 5864
HaeII r_GCGC'y
Cuts at: 1678 2045 2114 2842 4980 5143 7524 1678
Size: 367 69 728 2138 163 2381 2531
Fragments arranged by size:
2531 2381 2138 728 367 163 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2177 2209 2213 2245 2273
Size: 32 135 32 605 32 4 32 28
Cuts at: 2273 2305 2804 2836 2991 3023 7018 7050 7092
Size: 32 499 32 155 32 3995 32 42
Cuts at: 7092 7124 1373
Size: 32 2626
Fragments arranged by size:
3995 2626 605 499 155 135 42 32
32 32 32 32 32 32 32 32
28 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2209 2899 3940 5721 5967 669
Size: 988 281 271 690 1041 1781 246 3079
Fragments arranged by size:
3079 1781 1041 988 690 281 271 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8377
HpaI GTT'AAC
Cuts at: 5967 5967
Size: 8377
KpnI G_GTAC'C
Cuts at: 5606 5702 5606
Size: 96 8281
MfeI C'AATT_G
Cuts at: 3018 5976 3018
Size: 2958 5419
MluI A'CGCG_T
Cuts at: 8253 8253
Size: 8377
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3753 4144 4805 5694 7491 7675 1305
Size: 2448 391 661 889 1797 184 2007
Fragments arranged by size:
2448 2007 1797 889 661 391 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2925 3765 10
Size: 54 1425 343 1093 840 4622
Fragments arranged by size:
4622 1425 1093 840 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2083 2748 2862 2925 2945 4364 4969
Size: 648 917 665 114 63 20 1419 605
Cuts at: 4969 5007 6335 6694 6853 518
Size: 38 1328 359 159 2042
Fragments arranged by size:
2042 1419 1328 917 665 648 605 359
159 114 63 38 20
MspA1I CmG'CkG
Cuts at: 1606 2105 2150 2254 3252 4794 5074 5391 5427
Size: 499 45 104 998 1542 280 317 36
Cuts at: 5427 5793 6470 7618 7863 1606
Size: 366 677 1148 245 2120
Fragments arranged by size:
2120 1542 1148 998 677 499 366 317
280 245 104 45 36
NaeI GCC'GGC
Cuts at: 5477 5481 5477
Size: 4 8373
NcoI C'CATG_G
Cuts at: 513 1065 2091 2920 3760 513
Size: 552 1026 829 840 5130
Fragments arranged by size:
5130 1026 840 829 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8377
NgoMIV G'CCGG_C
Cuts at: 5475 5479 5475
Size: 4 8373
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8377
NotI GC'GGCC_GC
Cuts at: 5824 5824
Size: 8377
NsiI A_TGCA'T
Cuts at: 5121 5121
Size: 8377
NspI r_CATG'y
Cuts at: 1132 1229 2773 3352 3595 3706 5205 5735 7280
Size: 97 1544 579 243 111 1499 530 1545
Cuts at: 7280 1132
Size: 2229
Fragments arranged by size:
2229 1545 1544 1499 579 530 243 111
97
PasI CC'CwG_GG
Cuts at: 1348 4986 1348
Size: 3638 4739
PciI A'CATG_T
Cuts at: 3348 3702 5201 7276 3348
Size: 354 1499 2075 4449
Fragments arranged by size:
4449 2075 1499 354
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3863 1108
Size: 2755 5622
PfoI T'CCnGG_A
Cuts at: 3182 3800 3182
Size: 618 7759
PmeI GTTT'AAAC
Cuts at: 5681 5681
Size: 8377
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6446 6478 7959 7991 6446
Size: 32 1481 32 6832
Fragments arranged by size:
6832 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2096 2659 2853 3804 5375 2096
Size: 563 194 951 1571 5098
Fragments arranged by size:
5098 1571 951 563 194
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8377
PsiI TTA'TAA
Cuts at: 5947 5947
Size: 8377
PspOMI G'GGCC_C
Cuts at: 4982 4982
Size: 8377
PspXI vC'TCGA_Gb
Cuts at: 5380 5380
Size: 8377
PstI C_TGCA'G
Cuts at: 830 3428 4823 5166 5729 830
Size: 2598 1395 343 563 3478
Fragments arranged by size:
3478 2598 1395 563 343
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8377
PvuII CAG'CTG
Cuts at: 3252 4794 5074 5391 5427 3252
Size: 1542 280 317 36 6202
Fragments arranged by size:
6202 1542 317 280 36
SacII CC_GC'GG
Cuts at: 2151 2151
Size: 8377
SalI G'TCGA_C
Cuts at: 1936 2897 5719 1936
Size: 961 2822 4594
Fragments arranged by size:
4594 2822 961
SanDI GG'GwC_CC
Cuts at: 2096 5375 2096
Size: 3279 5098
SapI GCTCTTCn'nnn_
Cuts at: 2257 2257
Size: 8377
SbfI CC_TGCA'GG
Cuts at: 5729 5729
Size: 8377
ScaI AGT'ACT
Cuts at: 6625 8337 6625
Size: 1712 6665
SexAI A'CCwGG_T
Cuts at: 2187 5598 2187
Size: 3411 4966
SfcI C'TryA_G
Cuts at: 826 1045 3424 4456 4693 4819 5162 5725 6860
Size: 219 2379 1032 237 126 343 563 1135
Cuts at: 6860 7541 7732 826
Size: 681 191 1471
Fragments arranged by size:
2379 1471 1135 1032 681 563 343 237
219 191 126
SfiI GGCCn_nnn'nGGCC
Cuts at: 5522 5522
Size: 8377
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8377
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8377
SmaI CCC'GGG
Cuts at: 1872 3929 4551 5704 1872
Size: 2057 622 1153 4545
Fragments arranged by size:
4545 2057 1153 622
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2671 3088 3462 4017 5380 6481
Size: 197 926 728 417 374 555 1363 1101
Cuts at: 6481 7382 7644 820
Size: 901 262 1553
Fragments arranged by size:
1553 1363 1101 926 901 728 555 417
374 262 197
SnaBI TAC'GTA
Cuts at: 493 6168 493
Size: 5675 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8377
SphI G_CATG'C
Cuts at: 1132 3595 5735 1132
Size: 2463 2140 3774
Fragments arranged by size:
3774 2463 2140
SspI AAT'ATT
Cuts at: 5 52 6301 5
Size: 47 6249 2081
Fragments arranged by size:
6249 2081 47
StuI AGG'CCT
Cuts at: 1517 4943 1517
Size: 3426 4951
StyI C'CwwG_G
Cuts at: 513 1065 2091 2497 2590 2784 2886 2920 3760
Size: 552 1026 406 93 194 102 34 840
Cuts at: 3760 4180 5816 513
Size: 420 1636 3074
Fragments arranged by size:
3074 1636 1026 840 552 420 406 194
102 93 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3235 3773 4604 6404 6421 6574 6759
Size: 1255 1655 538 831 1800 17 153 185
Cuts at: 6759 7204 325
Size: 445 1498
Fragments arranged by size:
1800 1655 1498 1255 831 538 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2616 6623 8335
Size: 274 80 33 51 1443 639 4007 1712
Cuts at: 8335 96
Size: 138
Fragments arranged by size:
4007 1712 1443 639 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2456 2488 3711 3743 3862 3894 4537 4569 4798
Size: 32 1223 32 119 32 643 32 229
Cuts at: 4798 4830 8337 8369 2456
Size: 32 3507 32 2464
Fragments arranged by size:
3507 2464 1223 643 229 119 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2189 4175 5087 5449 1316
Size: 873 1986 912 362 4244
Fragments arranged by size:
4244 1986 912 873 362
XbaI T'CTAG_A
Cuts at: 3037 5713 3037
Size: 2676 5701
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2356 2659 3110 4579 4798 5314 2356
Size: 303 451 1469 219 516 5419
Fragments arranged by size:
5419 1469 516 451 303 219
XhoI C'TCGA_G
Cuts at: 1943 5380 1943
Size: 3437 4940
XmaI C'CCGG_G
Cuts at: 1870 3927 4549 5702 1870
Size: 2057 622 1153 4545
Fragments arranged by size:
4545 2057 1153 622
XmnI GAAnn'nnTTC
Cuts at: 5225 6506 5225
Size: 1281 7096
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4204 6185 276
Size: 53 83 186 936 2670 1981 2468
Fragments arranged by size:
2670 2468 1981 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BsiWI BstZ17I EcoICRI
EcoRV FspAI KasI NarI NruI PacI PmlI PsrI
RsrII SacI SfoI SrfI SwaI