Restriction map for
pFN21AB7295
(Circular) (Six-base) MAPSORT of: pFN21AB7295.seq Check: 4387 from: 1 to: 7372
pFN21AB7295 7372 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3371 3439 4216 4312 3371
Size: 68 777 96 6431
Fragments arranged by size:
6431 777 96 68
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5182 278
Size: 53 83 186 936 3646 2468
Fragments arranged by size:
3646 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2952 4715 1937
Size: 1015 1763 4594
Fragments arranged by size:
4594 1763 1015
Acc65I G'GTAC_C
Cuts at: 4693 4693
Size: 7372
AclI AA'CG_TT
Cuts at: 1563 5499 5872 1563
Size: 3936 373 3063
Fragments arranged by size:
3936 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2617 5435 6819 7087 1632
Size: 985 2818 1384 268 1917
Fragments arranged by size:
2818 1917 1384 985 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7175
AflIII A'CryG_T
Cuts at: 1203 3462 6271 7248 1203
Size: 2259 2809 977 1327
Fragments arranged by size:
2809 2259 1327 977
AgeI A'CCGG_T
Cuts at: 6203 6203
Size: 7372
AhdI GACnn_n'nnGTC
Cuts at: 2890 6101 2890
Size: 3211 4161
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7372
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3143 1184
Size: 1959 5413
AlwNI CAG_nnn'CTG
Cuts at: 3163 3409 5115 6687 3163
Size: 246 1706 1572 3848
Fragments arranged by size:
3848 1706 1572 246
ApaI G_GGCC'C
Cuts at: 2144 2151 2346 2354 2367 2406 2456 3579 4299
Size: 7 195 8 13 39 50 1123 720
Cuts at: 4299 4326 4422 4554 2144
Size: 27 96 132 4962
Fragments arranged by size:
4962 1123 720 195 132 96 50 39
27 13 8 7
ApaLI G'TGCA_C
Cuts at: 5429 6585 5429
Size: 1156 6216
ApoI r'AATT_y
Cuts at: 1493 4003 4681 4907 5059 7168 1493
Size: 2510 678 226 152 2109 1697
Fragments arranged by size:
2510 2109 1697 678 226 152
AscI GG'CGCG_CC
Cuts at: 3952 4350 3952
Size: 398 6974
AseI AT'TA_AT
Cuts at: 160 1032 5926 160
Size: 872 4894 1606
Fragments arranged by size:
4894 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7372
AvaI C'yCGr_G
Cuts at: 1870 1943 2033 2044 2137 2144 2172 2266 2345
Size: 73 90 11 93 7 28 94 79
Cuts at: 2345 2398 2840 3308 3846 4042 4291 4319 4345
Size: 53 442 468 538 196 249 28 26
Cuts at: 4345 4365 4566 4697 1870
Size: 20 201 131 4545
Fragments arranged by size:
4545 538 468 442 249 201 196 131
94 93 90 79 73 53 28 28
26 20 11 7
BamHI G'GATC_C
Cuts at: 4702 5069 4702
Size: 367 7005
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2261 3166 3304 3356 3423
Size: 325 217 627 474 905 138 52 67
Cuts at: 3423 3728 4369 4493 4613 4693 6148 618
Size: 305 641 124 120 80 1455 1842
Fragments arranged by size:
1842 1455 905 641 627 474 325 305
217 138 124 120 80 67 52
BanII G_rGCy'C
Cuts at: 1391 2045 2144 2151 2346 2354 2367 2406 2456
Size: 654 99 7 195 8 13 39 50
Cuts at: 2456 3570 3579 4299 4326 4422 4554 4691 1391
Size: 1114 9 720 27 96 132 137 4072
Fragments arranged by size:
4072 1114 720 654 195 137 132 99
96 50 39 27 13 9 8 7
BbeI G_GCGC'C
Cuts at: 3308 3427 4497 4617 3308
Size: 119 1070 120 6063
Fragments arranged by size:
6063 1070 120 119
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3688 928
Size: 971 1789 4612
Fragments arranged by size:
4612 1789 971
BbvCI CC'TCA_GC
Cuts at: 2999 2999
Size: 7372
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2791 2825 2857 2891 5563 5597 1903
Size: 34 854 34 32 34 2672 34 3678
Fragments arranged by size:
3678 2672 854 34 34 34 34 32
BciVI GTATCCnnnnn_n'
Cuts at: 5264 6474 5264
Size: 1210 6162
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7372
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2220 2259 2301
Size: 107 122 71 631 605 548 39 42
Cuts at: 2301 2505 4294 4347 5983 136
Size: 204 1789 53 1636 1525
Fragments arranged by size:
1789 1636 1525 631 605 548 204 122
107 71 53 42 39
BglII A'GATC_T
Cuts at: 3060 3831 7367 3060
Size: 771 3536 3065
Fragments arranged by size:
3536 3065 771
BlpI GC'TnA_GC
Cuts at: 3341 3902 4789 3341
Size: 561 887 5924
Fragments arranged by size:
5924 887 561
Bme1580I G_kGCm'C
Cuts at: 1790 2144 2151 2346 2354 2367 2406 2456 3579
Size: 354 7 195 8 13 39 50 1123
Cuts at: 3579 4299 4326 4372 4422 4554 5433 6589 1790
Size: 720 27 46 50 132 879 1156 2573
Fragments arranged by size:
2573 1156 1123 879 720 354 195 132
50 50 46 39 27 13 8 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7372
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2653 2687 3151
Size: 416 53 83 399 24 1236 34 464
Cuts at: 3151 5103 6061 442
Size: 1952 958 1753
Fragments arranged by size:
1952 1753 1236 958 464 416 399 83
53 34 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7372
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3950 6032 1239
Size: 2711 2082 2579
Fragments arranged by size:
2711 2579 2082
Bpu10I CC'TnA_GC
Cuts at: 2999 4442 2999
Size: 1443 5929
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3668 5497 6362 6660 3668
Size: 1829 865 298 4380
Fragments arranged by size:
4380 1829 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2714 2758 6035 882
Size: 617 1215 44 3277 2219
Fragments arranged by size:
3277 2219 1215 617 44
BsaAI yAC'GTr
Cuts at: 493 1204 2871 5163 493
Size: 711 1667 2292 2702
Fragments arranged by size:
2702 2292 1667 711
BsaBI GATnn'nnATC
Cuts at: 3101 7366 3101
Size: 4265 3107
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2526 3305 3424 4494
Size: 53 83 186 936 993 779 119 1070
Cuts at: 4494 4614 5179 5561 6196 275
Size: 120 565 382 635 1451
Fragments arranged by size:
1451 1070 993 936 779 635 565 382
186 120 119 83 53
BsaWI w'CCGG_w
Cuts at: 5805 6203 6477 6624 7035 5805
Size: 398 274 147 411 6142
Fragments arranged by size:
6142 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7147
Fragments arranged by size:
7147 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2444 2459 3071 3406 3602 3947 1186
Size: 1258 15 612 335 196 345 4611
Fragments arranged by size:
4611 1258 612 345 335 196 15
BseYI C'CCAG_C
Cuts at: 2160 3198 4123 4415 4580 6575 7071 2160
Size: 1038 925 292 165 1995 496 2461
Fragments arranged by size:
2461 1995 1038 925 496 292 165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2350 4001 4046 4130 1163
Size: 573 614 1651 45 84 4405
Fragments arranged by size:
4405 1651 614 573 84 45
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2254 3298 3979 4099 4238 4822
Size: 275 186 253 1044 681 120 139 584
Cuts at: 4822 5583 6611 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1044 1028 761 681 584 275 253
186 139 120
BsiHKAI G_wGCw'C
Cuts at: 2282 3522 3570 3780 5433 5518 6589 2282
Size: 1240 48 210 1653 85 1071 3065
Fragments arranged by size:
3065 1653 1240 1071 210 85 48
BsmI GAATG_Cn'
Cuts at: 4883 4976 4883
Size: 93 7279
BsmBI CGTCTCn'nnnn_
Cuts at: 3956 3956
Size: 7372
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2144 2151 2282 2346 2354 2367
Size: 399 255 99 7 131 64 8 13
Cuts at: 2367 2406 2456 3522 3570 3579 3780 4299 4326
Size: 39 50 1066 48 9 201 519 27
Cuts at: 4326 4372 4422 4554 4691 5433 5518 6589 1391
Size: 46 50 132 137 742 85 1071 2174
Fragments arranged by size:
2174 1071 1066 742 519 399 255 201
137 132 131 99 85 64 50 50
48 46 39 27 13 9 8 7
BspEI T'CCGG_A
Cuts at: 7035 7035
Size: 7372
BspHI T'CATG_A
Cuts at: 5261 6991 5261
Size: 1730 5642
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3371 3439 4159 4216 4312 4727 844
Size: 2527 68 720 57 96 415 3489
Fragments arranged by size:
3489 2527 720 415 96 68 57
BsrBI CCG'CTC
Cuts at: 1394 1823 4240 5259 1394
Size: 429 2417 1019 3507
Fragments arranged by size:
3507 2417 1019 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5867 6041 65
Size: 1183 4619 174 1396
Fragments arranged by size:
4619 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2223 2422 2798 3297 4619 6016 6203
Size: 110 413 199 376 499 1322 1397 187
Cuts at: 6203 1700
Size: 2869
Fragments arranged by size:
2869 1397 1322 499 413 376 199 187
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7372
BssHII G'CGCG_C
Cuts at: 1925 2012 2508 3952 4350 4433 4646 1925
Size: 87 496 1444 398 83 213 4651
Fragments arranged by size:
4651 1444 496 398 213 87 83
BssSI C'ACGA_G
Cuts at: 2245 2380 3229 4241 4274 5432 6444 2245
Size: 135 849 1012 33 1158 1012 3173
Fragments arranged by size:
3173 1158 1012 1012 849 135 33
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4024 794
Size: 3230 4142
BstBI TT'CG_AA
Cuts at: 6176 6176
Size: 7372
BstEII G'GTnAC_C
Cuts at: 1184 4171 1184
Size: 2987 4385
BstXI CCAn_nnnn'nTGG
Cuts at: 2905 2905
Size: 7372
BstYI r'GATC_y
Cuts at: 1972 2994 3060 3102 3501 3831 4702 5069 5454
Size: 1022 66 42 399 330 871 367 385
Cuts at: 5454 5471 6923 7009 7021 7367 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1022 871 399 385 367 346
330 86 66 42 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2216 2337 2386 2568 2732 2904
Size: 552 939 212 121 49 182 164 172
Cuts at: 2904 4373 4506 513
Size: 1469 133 3379
Fragments arranged by size:
3379 1469 939 552 212 182 172 164
133 121 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4168 487
Size: 683 56 2942 3691
Fragments arranged by size:
3691 2942 683 56
BtsI GCAGTG_nn'
Cuts at: 2333 2724 3390 4888 5682 5702 2333
Size: 391 666 1498 794 20 4003
Fragments arranged by size:
4003 1498 794 666 391 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7372
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7337
DraI TTT'AAA
Cuts at: 4676 4676
Size: 7372
DraIII CAC_nnn'GTG
Cuts at: 3766 4024 3766
Size: 258 7114
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2948 6379 809
Size: 507 1632 3431 1802
Fragments arranged by size:
3431 1802 1632 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2251 2283 3084
Size: 54 1425 50 275 18 421 32 801
Cuts at: 3084 3295 3382 3666 3969 4179 4206 4235 4524
Size: 211 87 284 303 210 27 29 289
Cuts at: 4524 4819 5708 7337 8
Size: 295 889 1629 43
Fragments arranged by size:
1629 1425 889 801 421 303 295 289
284 275 211 210 87 54 50 43
32 29 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 2251 3295 4235 4819 1537
Size: 275 439 1044 940 584 4090
Fragments arranged by size:
4090 1044 940 584 439 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3263 4148 5302 1353
Size: 1910 885 1154 3423
Fragments arranged by size:
3423 1910 1154 885
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2216 2441 3482 4456 5959 6333
Size: 1064 138 627 225 1041 974 1503 374
Cuts at: 6333 6479 387
Size: 146 1280
Fragments arranged by size:
1503 1280 1064 1041 974 627 374 225
146 138
EcoICRI GAG'CTC
Cuts at: 3568 3568
Size: 7372
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2617 3950 5435 6032 6819 7087 1239
Size: 393 985 1333 1485 597 787 268 1524
Fragments arranged by size:
1524 1485 1333 985 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3584 4019 3584
Size: 435 6937
EcoO109I rG'GnC_Cy
Cuts at: 2141 2342 2350 2363 2402 2604 3576 4295 4323
Size: 201 8 13 39 202 972 719 28
Cuts at: 4323 4550 2141
Size: 227 4963
Fragments arranged by size:
4963 972 719 227 202 201 39 28
13 8
EcoRI G'AATT_C
Cuts at: 4003 4681 5059 4003
Size: 678 378 6316
Fragments arranged by size:
6316 678 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3766 3766
Size: 7372
FspI TGC'GCA
Cuts at: 2867 2975 5878 2867
Size: 108 2903 4361
Fragments arranged by size:
4361 2903 108
HaeII r_GCGC'y
Cuts at: 1678 2500 3308 3427 4259 4497 4617 6519 1678
Size: 822 808 119 832 238 120 1902 2531
Fragments arranged by size:
2531 1902 832 822 808 238 120 119
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2876 2908 3094 3126 4025
Size: 32 135 32 1304 32 186 32 899
Cuts at: 4025 4057 4589 4621 6013 6045 6087 6119 1373
Size: 32 532 32 1392 32 42 32 2626
Fragments arranged by size:
2626 1392 1304 899 532 186 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2530 3598 4716 4962 669
Size: 988 281 592 1068 1118 246 3079
Fragments arranged by size:
3079 1118 1068 988 592 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7372
HpaI GTT'AAC
Cuts at: 4962 4962
Size: 7372
KasI G'GCGC_C
Cuts at: 3304 3423 4493 4613 3304
Size: 119 1070 120 6063
Fragments arranged by size:
6063 1070 120 119
KpnI G_GTAC'C
Cuts at: 4697 4697
Size: 7372
MfeI C'AATT_G
Cuts at: 4971 4971
Size: 7372
MluI A'CGCG_T
Cuts at: 7248 7248
Size: 7372
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4689 6486 6670 1305
Size: 3384 1797 184 2007
Fragments arranged by size:
3384 2007 1797 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3086 3971 10
Size: 54 1425 343 1254 885 3411
Fragments arranged by size:
3411 1425 1254 885 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2693 2903 3119 3350 3668 5330 5689
Size: 648 1527 210 216 231 318 1662 359
Cuts at: 5689 5848 518
Size: 159 2042
Fragments arranged by size:
2042 1662 1527 648 359 318 231 216
210 159
MspA1I CmG'CkG
Cuts at: 1606 2218 2339 2388 3485 3634 4252 4375 4508
Size: 612 121 49 1097 149 618 123 133
Cuts at: 4508 4562 4788 5465 6613 6858 1606
Size: 54 226 677 1148 245 2120
Fragments arranged by size:
2120 1148 1097 677 618 612 245 226
149 133 123 121 54 49
NaeI GCC'GGC
Cuts at: 2225 2424 3299 4621 2225
Size: 199 875 1322 4976
Fragments arranged by size:
4976 1322 875 199
NarI GG'CG_CC
Cuts at: 3305 3424 4494 4614 3305
Size: 119 1070 120 6063
Fragments arranged by size:
6063 1070 120 119
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5881
Fragments arranged by size:
5881 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7372
NgoMIV G'CCGG_C
Cuts at: 2223 2422 3297 4619 2223
Size: 199 875 1322 4976
Fragments arranged by size:
4976 1322 875 199
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7372
NotI GC'GGCC_GC
Cuts at: 2251 4235 4819 2251
Size: 1984 584 4804
Fragments arranged by size:
4804 1984 584
NspI r_CATG'y
Cuts at: 1132 1229 3280 3466 4730 6275 1132
Size: 97 2051 186 1264 1545 2229
Fragments arranged by size:
2229 2051 1545 1264 186 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7372
PciI A'CATG_T
Cuts at: 3462 6271 3462
Size: 2809 4563
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3409 1108
Size: 2301 5071
PfoI T'CCnGG_A
Cuts at: 2832 4077 2832
Size: 1245 6127
PmeI GTTT'AAAC
Cuts at: 4676 4676
Size: 7372
PmlI CAC'GTG
Cuts at: 2871 2871
Size: 7372
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5441 5473 6954 6986 5441
Size: 32 1481 32 5827
Fragments arranged by size:
5827 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7372
PsiI TTA'TAA
Cuts at: 4942 4942
Size: 7372
PspOMI G'GGCC_C
Cuts at: 2140 2147 2342 2350 2363 2402 2452 3575 4295
Size: 7 195 8 13 39 50 1123 720
Cuts at: 4295 4322 4418 4550 2140
Size: 27 96 132 4962
Fragments arranged by size:
4962 1123 720 195 132 96 50 39
27 13 8 7
PstI C_TGCA'G
Cuts at: 830 2520 3159 3180 4197 4724 830
Size: 1690 639 21 1017 527 3478
Fragments arranged by size:
3478 1690 1017 639 527 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7372
PvuII CAG'CTG
Cuts at: 3485 3485
Size: 7372
RsrII CG'GwC_CG
Cuts at: 4588 4588
Size: 7372
SacI G_AGCT'C
Cuts at: 3570 3570
Size: 7372
SacII CC_GC'GG
Cuts at: 2219 2340 2389 4376 4509 2219
Size: 121 49 1987 133 5082
Fragments arranged by size:
5082 1987 133 121 49
SalI G'TCGA_C
Cuts at: 1936 4714 1936
Size: 2778 4594
SbfI CC_TGCA'GG
Cuts at: 4724 4724
Size: 7372
ScaI AGT'ACT
Cuts at: 5620 7332 5620
Size: 1712 5660
SexAI A'CCwGG_T
Cuts at: 2778 2778
Size: 7372
SfcI C'TryA_G
Cuts at: 826 1045 2516 3155 3176 4193 4720 5855 6536
Size: 219 1471 639 21 1017 527 1135 681
Cuts at: 6536 6727 826
Size: 191 1471
Fragments arranged by size:
1471 1471 1135 1017 681 639 527 219
191 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 2220 2220
Size: 7372
SfoI GGC'GCC
Cuts at: 3306 3425 4495 4615 3306
Size: 119 1070 120 6063
Fragments arranged by size:
6063 1070 120 119
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7372
SgrAI Cr'CCGG_yG
Cuts at: 1700 4619 1700
Size: 2919 4453
SmaI CCC'GGG
Cuts at: 1872 2035 2046 2139 2146 2174 2268 3848 4293
Size: 163 11 93 7 28 94 1580 445
Cuts at: 4293 4321 4347 4367 4568 4699 1872
Size: 28 26 20 201 131 4545
Fragments arranged by size:
4545 1580 445 201 163 131 94 93
28 28 26 20 11 7
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3683 5476 6377 6639 820
Size: 197 926 1740 1793 901 262 1553
Fragments arranged by size:
1793 1740 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5163 493
Size: 4670 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7372
SphI G_CATG'C
Cuts at: 1132 4730 1132
Size: 3598 3774
SrfI GCCC'GGGC
Cuts at: 2046 4568 2046
Size: 2522 4850
SspI AAT'ATT
Cuts at: 5 52 5296 5
Size: 47 5244 2081
Fragments arranged by size:
5244 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7372
StyI C'CwwG_G
Cuts at: 513 1065 2004 2286 2367 4811 513
Size: 552 939 282 81 2444 3074
Fragments arranged by size:
3074 2444 939 552 282 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3190 3344 5399 5416 5569 5754 6199
Size: 1255 1610 154 2055 17 153 185 445
Cuts at: 6199 325
Size: 1498
Fragments arranged by size:
2055 1610 1498 1255 445 185 154 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3243 3441 3459
Size: 274 80 33 51 1443 1266 198 18
Cuts at: 3459 3747 5618 7330 96
Size: 288 1871 1712 138
Fragments arranged by size:
1871 1712 1443 1266 288 274 198 138
80 51 33 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3018 3050 7332 7364 3018
Size: 32 4282 32 3026
Fragments arranged by size:
4282 3026 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7372
XbaI T'CTAG_A
Cuts at: 4708 4708
Size: 7372
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3214 3979 4543 3214
Size: 765 564 6043
Fragments arranged by size:
6043 765 564
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7372
XmaI C'CCGG_G
Cuts at: 1870 2033 2044 2137 2144 2172 2266 3846 4291
Size: 163 11 93 7 28 94 1580 445
Cuts at: 4291 4319 4345 4365 4566 4697 1870
Size: 28 26 20 201 131 4545
Fragments arranged by size:
4545 1580 445 201 163 131 94 93
28 28 26 20 11 7
XmnI GAAnn'nnTTC
Cuts at: 5501 5501
Size: 7372
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5180 276
Size: 53 83 186 936 3646 2468
Fragments arranged by size:
3646 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AvrII BaeI BfrBI BplI BsiWI BstZ17I
Bsu36I EcoRV FseI FspAI NruI NsiI PacI PpuMI
PspXI PsrI SanDI SapI SwaI