Restriction map for
pFN21AB7289
(Circular) (Six-base) MAPSORT of: pFN21AB7289.seq Check: 5027 from: 1 to: 7669
pFN21AB7289 7669 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5479 278
Size: 53 83 186 936 3943 2468
Fragments arranged by size:
3943 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5012 1937
Size: 3075 4594
Acc65I G'GTAC_C
Cuts at: 4990 4990
Size: 7669
AclI AA'CG_TT
Cuts at: 1563 3369 5796 6169 1563
Size: 1806 2427 373 3063
Fragments arranged by size:
3063 2427 1806 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2417 2509 2633 3059 4422 5732 7116 7384
Size: 785 92 124 426 1363 1310 1384 268
Cuts at: 7384 1632
Size: 1917
Fragments arranged by size:
1917 1384 1363 1310 785 426 268 124
92
AfeI AGC'GCT
Cuts at: 3597 3597
Size: 7669
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7472
AflIII A'CryG_T
Cuts at: 1203 4392 6568 7545 1203
Size: 3189 2176 977 1327
Fragments arranged by size:
3189 2176 1327 977
AgeI A'CCGG_T
Cuts at: 6500 6500
Size: 7669
AhdI GACnn_n'nnGTC
Cuts at: 6398 6398
Size: 7669
AleI CACnn'nnGTG
Cuts at: 1166 3315 3632 1166
Size: 2149 317 5203
Fragments arranged by size:
5203 2149 317
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7669
AlwNI CAG_nnn'CTG
Cuts at: 2488 2727 3721 4283 4315 5412 6984 2488
Size: 239 994 562 32 1097 1572 3173
Fragments arranged by size:
3173 1572 1097 994 562 239 32
ApaI G_GGCC'C
Cuts at: 2705 4922 2705
Size: 2217 5452
ApaLI G'TGCA_C
Cuts at: 5726 6882 5726
Size: 1156 6513
ApoI r'AATT_y
Cuts at: 1493 2747 4978 5204 5356 7465 1493
Size: 1254 2231 226 152 2109 1697
Fragments arranged by size:
2231 2109 1697 1254 226 152
AscI GG'CGCG_CC
Cuts at: 4101 4101
Size: 7669
AseI AT'TA_AT
Cuts at: 160 1032 6223 160
Size: 872 5191 1606
Fragments arranged by size:
5191 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7669
AvaI C'yCGr_G
Cuts at: 1870 1943 3360 4118 4994 1870
Size: 73 1417 758 876 4545
Fragments arranged by size:
4545 1417 876 758 73
BamHI G'GATC_C
Cuts at: 4999 5366 4999
Size: 367 7302
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3605 4699 4990 6445 618
Size: 325 217 627 1818 1094 291 1455 1842
Fragments arranged by size:
1842 1818 1455 1094 627 325 291 217
BanII G_rGCy'C
Cuts at: 1391 2705 3084 3956 4095 4236 4342 4818 4922
Size: 1314 379 872 139 141 106 476 104
Cuts at: 4922 4988 1391
Size: 66 4072
Fragments arranged by size:
4072 1314 872 476 379 141 139 106
104 66
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2252 2358 2594 4161 928
Size: 971 353 106 236 1567 4436
Fragments arranged by size:
4436 1567 971 353 236 106
BbvCI CC'TCA_GC
Cuts at: 2741 3149 2741
Size: 408 7261
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5860 5894 1903
Size: 34 3923 34 3678
Fragments arranged by size:
3923 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2321 3192 5561 6771 2321
Size: 871 2369 1210 3219
Fragments arranged by size:
3219 2369 1210 871
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7669
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2642 4240 6280
Size: 107 122 71 631 605 970 1598 2040
Cuts at: 6280 136
Size: 1525
Fragments arranged by size:
2040 1598 1525 970 631 605 122 107
71
BglII A'GATC_T
Cuts at: 7664 7664
Size: 7669
BlpI GC'TnA_GC
Cuts at: 3165 3797 4433 5086 3165
Size: 632 636 653 5748
Fragments arranged by size:
5748 653 636 632
Bme1580I G_kGCm'C
Cuts at: 1790 2705 4623 4702 4922 5730 6886 1790
Size: 915 1918 79 220 808 1156 2573
Fragments arranged by size:
2573 1918 1156 915 808 220 79
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7669
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2059 5400 6358
Size: 416 53 83 399 24 642 3341 958
Cuts at: 6358 442
Size: 1753
Fragments arranged by size:
3341 1753 958 642 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7669
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2899 2899
Size: 7669
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2114 2239 2422 2932 3270 3875 4092 4613
Size: 875 125 183 510 338 605 217 521
Cuts at: 4613 4886 6329 1239
Size: 273 1443 2579
Fragments arranged by size:
2579 1443 875 605 521 510 338 273
217 183 125
Bpu10I CC'TnA_GC
Cuts at: 2741 3149 4005 4574 2741
Size: 408 856 569 5836
Fragments arranged by size:
5836 856 569 408
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5794 6659 6957 5794
Size: 865 298 6506
Fragments arranged by size:
6506 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4781 6332 882
Size: 617 3282 1551 2219
Fragments arranged by size:
3282 2219 1551 617
BsaAI yAC'GTr
Cuts at: 493 1204 3836 4555 5460 493
Size: 711 2632 719 905 2702
Fragments arranged by size:
2702 2632 905 719 711
BsaBI GATnn'nnATC
Cuts at: 2283 3416 7663 2283
Size: 1133 4247 2289
Fragments arranged by size:
4247 2289 1133
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4581 5476 5858 6493
Size: 53 83 186 936 3048 895 382 635
Cuts at: 6493 275
Size: 1451
Fragments arranged by size:
3048 1451 936 895 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2142 4665 6102 6500 6774 6921 7332 2142
Size: 2523 1437 398 274 147 411 2479
Fragments arranged by size:
2523 2479 1437 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3608 3638 1178
Size: 30 165 30 2205 30 5209
Fragments arranged by size:
5209 2205 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2871 4610 4874 4910 4958 1186
Size: 1685 1739 264 36 48 3897
Fragments arranged by size:
3897 1739 1685 264 48 36
BseYI C'CCAG_C
Cuts at: 2468 3949 4082 4330 4922 6872 7368 2468
Size: 1481 133 248 592 1950 496 2769
Fragments arranged by size:
2769 1950 1481 592 496 248 133
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2077 4490 4658 1163
Size: 573 341 2413 168 4174
Fragments arranged by size:
4174 2413 573 341 168
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3968 5119 5880 6908 1540
Size: 275 186 1967 1151 761 1028 2301
Fragments arranged by size:
2301 1967 1151 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4342 5730 5815 6886 4342
Size: 1388 85 1071 5125
Fragments arranged by size:
5125 1388 1071 85
BsmI GAATG_Cn'
Cuts at: 5180 5273 5180
Size: 93 7576
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2705 3084 3956 4095 4236 4342 4623
Size: 399 915 379 872 139 141 106 281
Cuts at: 4623 4702 4818 4922 4988 5730 5815 6886 1391
Size: 79 116 104 66 742 85 1071 2174
Fragments arranged by size:
2174 1071 915 872 742 399 379 281
141 139 116 106 104 85 79 66
BspEI T'CCGG_A
Cuts at: 2142 4665 7332 2142
Size: 2523 2667 2479
Fragments arranged by size:
2667 2523 2479
BspHI T'CATG_A
Cuts at: 3399 5558 7288 3399
Size: 2159 1730 3780
Fragments arranged by size:
3780 2159 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4277 4737 5024 844
Size: 3433 460 287 3489
Fragments arranged by size:
3489 3433 460 287
BsrBI CCG'CTC
Cuts at: 1394 1823 3964 5556 1394
Size: 429 2141 1592 3507
Fragments arranged by size:
3507 2141 1592 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2715 3204 6164 6338 65
Size: 1183 1467 489 2960 174 1396
Fragments arranged by size:
2960 1467 1396 1183 489 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4113 6313 6500 1700
Size: 110 2303 2200 187 2869
Fragments arranged by size:
2869 2303 2200 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7669
BssHII G'CGCG_C
Cuts at: 1925 4018 4101 1925
Size: 2093 83 5493
Fragments arranged by size:
5493 2093 83
BssSI C'ACGA_G
Cuts at: 5729 6741 5729
Size: 1012 6657
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3175 794
Size: 2381 5288
BstBI TT'CG_AA
Cuts at: 3645 6473 3645
Size: 2828 4841
BstEII G'GTnAC_C
Cuts at: 1184 2497 2878 1184
Size: 1313 381 5975
Fragments arranged by size:
5975 1313 381
BstXI CCAn_nnnn'nTGG
Cuts at: 2564 3685 3709 2564
Size: 1121 24 6524
Fragments arranged by size:
6524 1121 24
BstYI r'GATC_y
Cuts at: 1972 2358 3558 4999 5366 5751 5768 7220 7306
Size: 386 1200 1441 367 385 17 1452 86
Cuts at: 7306 7318 7664 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1441 1200 386 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4734 4734
Size: 7669
BtgI C'CryG_G
Cuts at: 513 1065 2004 2265 2688 513
Size: 552 939 261 423 5494
Fragments arranged by size:
5494 939 552 423 261
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3095 487
Size: 683 56 1869 5061
Fragments arranged by size:
5061 1869 683 56
BtsI GCAGTG_nn'
Cuts at: 2089 2683 2970 2991 4321 5185 5979 5999 2089
Size: 594 287 21 1330 864 794 20 3759
Fragments arranged by size:
3759 1330 864 794 594 287 21 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7669
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2030 2065 2875 2910 529
Size: 35 1466 35 810 35 5288
Fragments arranged by size:
5288 1466 810 35 35 35
DraI TTT'AAA
Cuts at: 4973 4973
Size: 7669
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6676 809
Size: 507 5360 1802
Fragments arranged by size:
5360 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2191 2643 3423
Size: 54 1425 50 275 18 361 452 780
Cuts at: 3423 3510 3699 4252 4902 5116 6005 7634 8
Size: 87 189 553 650 214 889 1629 43
Fragments arranged by size:
1629 1425 889 780 650 553 452 361
275 214 189 87 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5116 1537
Size: 275 3304 4090
Fragments arranged by size:
4090 3304 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2528 2540 3395 4224 4398 4887 5599 1353
Size: 1175 12 855 829 174 489 712 3423
Fragments arranged by size:
3423 1175 855 829 712 489 174 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4634 6256 6630 6776 387
Size: 1064 138 3045 1622 374 146 1280
Fragments arranged by size:
3045 1622 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4340 4340
Size: 7669
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 2239 2417 2422 2509 2633 2932
Size: 393 482 125 178 5 87 124 299
Cuts at: 2932 3059 3270 3875 4092 4422 4613 4886 5732
Size: 127 211 605 217 330 191 273 846
Cuts at: 5732 6329 7116 7384 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 846 787 605 597 482 393 330
299 273 268 217 211 191 178 127
125 124 87 5
EcoNI CCTnn'n_nnAGG
Cuts at: 2324 4733 2324
Size: 2409 5260
EcoO109I rG'GnC_Cy
Cuts at: 2125 2156 2701 2702 3145 3979 4918 4919 2125
Size: 31 545 1 443 834 939 1 4875
Fragments arranged by size:
4875 939 834 545 443 31 1 1
EcoRI G'AATT_C
Cuts at: 4978 5356 4978
Size: 378 7291
EcoRV GAT'ATC
Cuts at: 2800 2800
Size: 7669
FseI GG_CCGG'CC
Cuts at: 4117 4117
Size: 7669
FspI TGC'GCA
Cuts at: 6175 6175
Size: 7669
HaeII r_GCGC'y
Cuts at: 1678 2532 2943 3599 3994 6816 1678
Size: 854 411 656 395 2822 2531
Fragments arranged by size:
2822 2531 854 656 411 395
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4287 4319 4379 4411 4666
Size: 32 135 32 2715 32 60 32 255
Cuts at: 4666 4698 6310 6342 6384 6416 1373
Size: 32 1612 32 42 32 2626
Fragments arranged by size:
2715 2626 1612 255 135 60 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3367 3925 5013 5259 669
Size: 988 281 1429 558 1088 246 3079
Fragments arranged by size:
3079 1429 1088 988 558 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7669
HpaI GTT'AAC
Cuts at: 5259 5259
Size: 7669
KpnI G_GTAC'C
Cuts at: 4994 4994
Size: 7669
MfeI C'AATT_G
Cuts at: 5268 5268
Size: 7669
MluI A'CGCG_T
Cuts at: 7545 7545
Size: 7669
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3948 4029 4836 6783 6967 1305
Size: 2643 81 807 1947 184 2007
Fragments arranged by size:
2643 2007 1947 807 184 81
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2193 2645 3425 3512 3701
Size: 54 1425 343 361 452 780 87 189
Cuts at: 3701 4254 10
Size: 553 3425
Fragments arranged by size:
3425 1425 780 553 452 361 343 189
87 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2234 2687 2756 3315 3431 3461 3512
Size: 648 1068 453 69 559 116 30 51
Cuts at: 3512 3632 3683 5627 5986 6145 518
Size: 120 51 1944 359 159 2042
Fragments arranged by size:
2042 1944 1068 648 559 453 359 159
120 116 69 51 51 30
MspA1I CmG'CkG
Cuts at: 1606 2400 3344 3632 4432 4486 5085 5762 6910
Size: 794 944 288 800 54 599 677 1148
Cuts at: 6910 7155 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 944 800 794 677 599 288
245 54
NaeI GCC'GGC
Cuts at: 4115 4115
Size: 7669
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6178
Fragments arranged by size:
6178 939 552
NdeI CA'TA_TG
Cuts at: 387 3868 387
Size: 3481 4188
NgoMIV G'CCGG_C
Cuts at: 4113 4113
Size: 7669
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7669
NotI GC'GGCC_GC
Cuts at: 5116 5116
Size: 7669
NspI r_CATG'y
Cuts at: 1132 1229 4396 5027 6572 1132
Size: 97 3167 631 1545 2229
Fragments arranged by size:
3167 2229 1545 631 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7669
PciI A'CATG_T
Cuts at: 4392 6568 4392
Size: 2176 5493
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2320 4033 4489 1108
Size: 1212 1713 456 4288
Fragments arranged by size:
4288 1713 1212 456
PfoI T'CCnGG_A
Cuts at: 3184 3184
Size: 7669
PmeI GTTT'AAAC
Cuts at: 4973 4973
Size: 7669
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5738 5770 7251 7283 5738
Size: 32 1481 32 6124
Fragments arranged by size:
6124 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7669
PsiI TTA'TAA
Cuts at: 5239 5239
Size: 7669
PspOMI G'GGCC_C
Cuts at: 2701 4918 2701
Size: 2217 5452
PstI C_TGCA'G
Cuts at: 830 2484 3726 3978 3990 4288 4717 5021 830
Size: 1654 1242 252 12 298 429 304 3478
Fragments arranged by size:
3478 1654 1242 429 304 298 252 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7669
PvuII CAG'CTG
Cuts at: 2400 3344 3632 4432 4486 2400
Size: 944 288 800 54 5583
Fragments arranged by size:
5583 944 800 288 54
SacI G_AGCT'C
Cuts at: 4342 4342
Size: 7669
SalI G'TCGA_C
Cuts at: 1936 5011 1936
Size: 3075 4594
SapI GCTCTTCn'nnn_
Cuts at: 4224 4224
Size: 7669
SbfI CC_TGCA'GG
Cuts at: 4288 5021 4288
Size: 733 6936
ScaI AGT'ACT
Cuts at: 3280 5917 7629 3280
Size: 2637 1712 3320
Fragments arranged by size:
3320 2637 1712
SfcI C'TryA_G
Cuts at: 826 1045 2480 3722 3974 3986 4284 4601 4713
Size: 219 1435 1242 252 12 298 317 112
Cuts at: 4713 5017 6152 6833 7024 826
Size: 304 1135 681 191 1471
Fragments arranged by size:
1471 1435 1242 1135 681 317 304 298
252 219 191 112 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7669
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7669
SmaI CCC'GGG
Cuts at: 1872 3362 4996 1872
Size: 1490 1634 4545
Fragments arranged by size:
4545 1634 1490
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5773 6674 6936 820
Size: 197 926 3830 901 262 1553
Fragments arranged by size:
3830 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5460 493
Size: 4967 2702
SpeI A'CTAG_T
Cuts at: 152 2509 152
Size: 2357 5312
SphI G_CATG'C
Cuts at: 1132 5027 1132
Size: 3895 3774
SspI AAT'ATT
Cuts at: 5 52 5593 5
Size: 47 5541 2081
Fragments arranged by size:
5541 2081 47
StuI AGG'CCT
Cuts at: 1517 2438 4415 4744 1517
Size: 921 1977 329 4442
Fragments arranged by size:
4442 1977 921 329
StyI C'CwwG_G
Cuts at: 513 1065 2004 2646 3781 4534 5108 513
Size: 552 939 642 1135 753 574 3074
Fragments arranged by size:
3074 1135 939 753 642 574 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3292 5696 5713 5866 6051 6496 325
Size: 1255 1712 2404 17 153 185 445 1498
Fragments arranged by size:
2404 1712 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3278 5915 7627
Size: 274 80 33 51 1443 1301 2637 1712
Cuts at: 7627 96
Size: 138
Fragments arranged by size:
2637 1712 1443 1301 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2316 2348 3061 3093 3608 3640 7629 7661 2316
Size: 32 713 32 515 32 3989 32 2324
Fragments arranged by size:
3989 2324 713 515 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2414 1316
Size: 1098 6571
XbaI T'CTAG_A
Cuts at: 5005 5005
Size: 7669
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2263 3016 4381 2263
Size: 753 1365 5551
Fragments arranged by size:
5551 1365 753
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7669
XmaI C'CCGG_G
Cuts at: 1870 3360 4994 1870
Size: 1490 1634 4545
Fragments arranged by size:
4545 1634 1490
XmnI GAAnn'nnTTC
Cuts at: 5798 5798
Size: 7669
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5477 276
Size: 53 83 186 936 3943 2468
Fragments arranged by size:
3943 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FseI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AvrII BaeI BbeI BfrBI BsiWI BsmBI
BstZ17I DraIII FalI FspAI KasI NarI NruI NsiI
PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI