Restriction map for
pFN21AB7288
(Circular) (Six-base) MAPSORT of: pFN21AB7288.seq Check: 8961 from: 1 to: 8338
pFN21AB7288 8338 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2376 3809 4263 4801 2376
Size: 1433 454 538 5913
Fragments arranged by size:
5913 1433 538 454
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6148 278
Size: 53 83 186 936 4612 2468
Fragments arranged by size:
4612 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4272 4533 4569 5681 1937
Size: 2335 261 36 1112 4594
Fragments arranged by size:
4594 2335 1112 261 36
Acc65I G'GTAC_C
Cuts at: 5659 5659
Size: 8338
AclI AA'CG_TT
Cuts at: 1563 6465 6838 1563
Size: 4902 373 3063
Fragments arranged by size:
4902 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4045 4604 6401 7785 8053 1632
Size: 2413 559 1797 1384 268 1917
Fragments arranged by size:
2413 1917 1797 1384 559 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8141
AflIII A'CryG_T
Cuts at: 1203 5355 7237 8214 1203
Size: 4152 1882 977 1327
Fragments arranged by size:
4152 1882 1327 977
AgeI A'CCGG_T
Cuts at: 7169 7169
Size: 8338
AhdI GACnn_n'nnGTC
Cuts at: 7067 7067
Size: 8338
AleI CACnn'nnGTG
Cuts at: 1166 2444 3743 5306 1166
Size: 1278 1299 1563 4198
Fragments arranged by size:
4198 1563 1299 1278
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3156 3969 4829 1184
Size: 1972 813 860 4693
Fragments arranged by size:
4693 1972 860 813
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2552 2584 2552
Size: 32 8306
AlwNI CAG_nnn'CTG
Cuts at: 2526 2683 3019 3370 4068 4167 5137 6081 7653
Size: 157 336 351 698 99 970 944 1572
Cuts at: 7653 2526
Size: 3211
Fragments arranged by size:
3211 1572 970 944 698 351 336 157
99
ApaI G_GGCC'C
Cuts at: 2151 3600 4436 4582 4953 2151
Size: 1449 836 146 371 5536
Fragments arranged by size:
5536 1449 836 371 146
ApaLI G'TGCA_C
Cuts at: 3511 3621 4865 6395 7551 3511
Size: 110 1244 1530 1156 4298
Fragments arranged by size:
4298 1530 1244 1156 110
ApoI r'AATT_y
Cuts at: 1493 5647 5873 6025 8134 1493
Size: 4154 226 152 2109 1697
Fragments arranged by size:
4154 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6892 160
Size: 872 5860 1606
Fragments arranged by size:
5860 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8338
AvaI C'yCGr_G
Cuts at: 1870 1943 2287 2402 2459 2666 2761 3448 3805
Size: 73 344 115 57 207 95 687 357
Cuts at: 3805 4482 4895 5663 1870
Size: 677 413 768 4545
Fragments arranged by size:
4545 768 687 677 413 357 344 207
115 95 73 57
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2855 2888 2855
Size: 33 8305
BamHI G'GATC_C
Cuts at: 2008 5668 6035 2008
Size: 3660 367 4311
Fragments arranged by size:
4311 3660 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2182 2398 2435 2927 3310
Size: 325 217 627 395 216 37 492 383
Cuts at: 3310 4445 4985 5659 7114 618
Size: 1135 540 674 1455 1842
Fragments arranged by size:
1842 1455 1135 674 627 540 492 395
383 325 217 216 37
BanII G_rGCy'C
Cuts at: 1391 2151 2263 2454 3600 4436 4582 4926 4953
Size: 760 112 191 1146 836 146 344 27
Cuts at: 4953 5561 5598 5657 1391
Size: 608 37 59 4072
Fragments arranged by size:
4072 1146 836 760 608 344 191 146
112 59 37 27
BbeI G_GCGC'C
Cuts at: 2402 3314 2402
Size: 912 7426
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4137 928
Size: 971 2238 5129
Fragments arranged by size:
5129 2238 971
BbvCI CC'TCA_GC
Cuts at: 2720 3208 2720
Size: 488 7850
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6529 6563 1903
Size: 34 4592 34 3678
Fragments arranged by size:
4592 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6230 7440 6230
Size: 1210 7128
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8338
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2155 2267 6949
Size: 107 122 71 631 605 483 112 4682
Cuts at: 6949 136
Size: 1525
Fragments arranged by size:
4682 1525 631 605 483 122 112 107
71
BglII A'GATC_T
Cuts at: 5172 8333 5172
Size: 3161 5177
BlpI GC'TnA_GC
Cuts at: 2519 2613 3371 5755 2519
Size: 94 758 2384 5102
Fragments arranged by size:
5102 2384 758 94
Bme1580I G_kGCm'C
Cuts at: 1790 2151 2160 2187 2438 2776 2932 3515 3600
Size: 361 9 27 251 338 156 583 85
Cuts at: 3600 3625 4187 4436 4582 4869 4953 5382 6399
Size: 25 562 249 146 287 84 429 1017
Cuts at: 6399 7555 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1017 583 562 429 361 338
287 251 249 156 146 85 84 27
25 9
BmgBI CAC'GTC
Cuts at: 1318 2485 2655 4345 5356 1318
Size: 1167 170 1690 1011 4300
Fragments arranged by size:
4300 1690 1167 1011 170
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4621 4954 5369
Size: 416 53 83 399 24 3204 333 415
Cuts at: 5369 6069 7027 442
Size: 700 958 1753
Fragments arranged by size:
3204 1753 958 700 416 415 399 333
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3032 3041 4102 4328 5428 5444 6998 1239
Size: 1793 9 1061 226 1100 16 1554 2579
Fragments arranged by size:
2579 1793 1554 1100 1061 226 16 9
Bpu10I CC'TnA_GC
Cuts at: 2720 2966 3208 2720
Size: 246 242 7850
Fragments arranged by size:
7850 246 242
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6463 7328 7626 6463
Size: 865 298 7175
Fragments arranged by size:
7175 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2352 3986 4492 4599 7001 882
Size: 617 853 1634 506 107 2402 2219
Fragments arranged by size:
2402 2219 1634 853 617 506 107
BsaAI yAC'GTr
Cuts at: 493 1204 2224 2863 3241 3728 5125 6129 493
Size: 711 1020 639 378 487 1397 1004 2702
Fragments arranged by size:
2702 1397 1020 1004 711 639 487 378
BsaBI GATnn'nnATC
Cuts at: 8332 8332
Size: 8338
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2199 2399 2736 3311
Size: 53 83 186 936 666 200 337 575
Cuts at: 3311 3918 4056 4275 4887 6145 6527 7162 275
Size: 607 138 219 612 1258 382 635 1451
Fragments arranged by size:
1451 1258 936 666 635 612 607 575
382 337 219 200 186 138 83 53
BsaWI w'CCGG_w
Cuts at: 2280 2550 3166 3522 5166 6771 7169 7443 7590
Size: 270 616 356 1644 1605 398 274 147
Cuts at: 7590 8001 2280
Size: 411 2617
Fragments arranged by size:
2617 1644 1605 616 411 398 356 274
270 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2552 2582 2644 2674 3188
Size: 30 165 30 1149 30 62 30 514
Cuts at: 3188 3218 3371 3401 3992 4022 4376 4406 1178
Size: 30 153 30 591 30 354 30 5110
Fragments arranged by size:
5110 1149 591 514 354 165 153 62
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2324 2327 2497 2573 2576 2579 2652 2759
Size: 1138 3 170 76 3 3 73 107
Cuts at: 2759 3196 3890 5060 1186
Size: 437 694 1170 4464
Fragments arranged by size:
4464 1170 1138 694 437 170 107 76
73 3 3 3
BseYI C'CCAG_C
Cuts at: 2818 3036 3244 3295 3441 3693 3921 4746 7541
Size: 218 208 51 146 252 228 825 2795
Cuts at: 7541 8037 2818
Size: 496 3119
Fragments arranged by size:
3119 2795 825 496 252 228 218 208
146 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2348 2861 3163 3341 3368 3440 3574
Size: 573 612 513 302 178 27 72 134
Cuts at: 3574 1163
Size: 5927
Fragments arranged by size:
5927 612 573 513 302 178 134 72
27
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4411 5555 5788 6549 7577 1540
Size: 275 186 2410 1144 233 761 1028 2301
Fragments arranged by size:
2410 2301 1144 1028 761 275 233 186
BsiHKAI G_wGCw'C
Cuts at: 2751 2862 3258 3515 3625 3912 3948 4604 4869
Size: 111 396 257 110 287 36 656 265
Cuts at: 4869 4926 4967 5007 5598 6399 6484 7555 2751
Size: 57 41 40 591 801 85 1071 3534
Fragments arranged by size:
3534 1071 801 656 591 396 287 265
257 111 110 85 57 41 40 36
BsmI GAATG_Cn'
Cuts at: 4745 4830 5849 5942 4745
Size: 85 1019 93 7141
Fragments arranged by size:
7141 1019 93 85
BsmBI CGTCTCn'nnnn_
Cuts at: 2475 4392 4409 2475
Size: 1917 17 6404
Fragments arranged by size:
6404 1917 17
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2151 2160 2187 2263 2438 2454 2751
Size: 399 361 9 27 76 175 16 297
Cuts at: 2751 2776 2862 2932 3258 3515 3600 3625 3912
Size: 25 86 70 326 257 85 25 287
Cuts at: 3912 3948 4187 4436 4582 4604 4869 4926 4953
Size: 36 239 249 146 22 265 57 27
Cuts at: 4953 4967 5007 5382 5561 5598 5657 6399 6484
Size: 14 40 375 179 37 59 742 85
Cuts at: 6484 7555 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 375 361 326 297
287 265 257 249 239 179 175 146
86 85 85 76 70 59 57 40
37 36 27 27 25 25 22 16
14 9
BspEI T'CCGG_A
Cuts at: 2280 2550 3522 5166 8001 2280
Size: 270 972 1644 2835 2617
Fragments arranged by size:
2835 2617 1644 972 270
BspHI T'CATG_A
Cuts at: 2886 6227 7957 2886
Size: 3341 1730 3267
Fragments arranged by size:
3341 3267 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2370 2376 2883 3390 3809 4263 4801 5078
Size: 1526 6 507 507 419 454 538 277
Cuts at: 5078 5693 844
Size: 615 3489
Fragments arranged by size:
3489 1526 615 538 507 507 454 419
277 6
BsrBI CCG'CTC
Cuts at: 1394 1823 2027 2083 2165 4860 4996 6225 1394
Size: 429 204 56 82 2695 136 1229 3507
Fragments arranged by size:
3507 2695 1229 429 204 136 82 56
BsrDI GCAATG_nn'
Cuts at: 65 1248 3482 4368 4888 6833 7007 65
Size: 1183 2234 886 520 1945 174 1396
Fragments arranged by size:
2234 1945 1396 1183 886 520 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2014 2489 2643 3152 3430 6982 7169
Size: 110 204 475 154 509 278 3552 187
Cuts at: 7169 1700
Size: 2869
Fragments arranged by size:
3552 2869 509 475 278 204 187 154
110
BsrGI T'GTAC_A
Cuts at: 96 5289 96
Size: 5193 3145
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8338
BssSI C'ACGA_G
Cuts at: 2393 3941 6398 7410 2393
Size: 1548 2457 1012 3321
Fragments arranged by size:
3321 2457 1548 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4725 794
Size: 3931 4407
BstBI TT'CG_AA
Cuts at: 7142 7142
Size: 8338
BstEII G'GTnAC_C
Cuts at: 1184 4197 1184
Size: 3013 5325
BstXI CCAn_nnnn'nTGG
Cuts at: 2446 3233 4381 4793 2446
Size: 787 1148 412 5991
Fragments arranged by size:
5991 1148 787 412
BstYI r'GATC_y
Cuts at: 1972 2008 4233 5104 5172 5668 6035 6420 6437
Size: 36 2225 871 68 496 367 385 17
Cuts at: 6437 7889 7975 7987 8333 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2225 1977 1452 871 496 385 367 346
86 68 36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2848 4374 4428 4441 4678 5016
Size: 552 939 844 1526 54 13 237 338
Cuts at: 5016 5391 5506 513
Size: 375 115 3345
Fragments arranged by size:
3345 1526 939 844 552 375 338 237
115 54 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2544 487
Size: 683 56 1318 6281
Fragments arranged by size:
6281 1318 683 56
BtsI GCAGTG_nn'
Cuts at: 3649 3887 4165 4876 5188 5854 6648 6668 3649
Size: 238 278 711 312 666 794 20 5319
Fragments arranged by size:
5319 794 711 666 312 278 238 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8338
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3259 3294 4594 4629 529
Size: 35 2695 35 1300 35 4238
Fragments arranged by size:
4238 2695 1300 35 35 35
DraI TTT'AAA
Cuts at: 3643 5642 3643
Size: 1999 6339
DraIII CAC_nnn'GTG
Cuts at: 2227 2227
Size: 8338
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4484 4544 4565 7345 809
Size: 507 3168 60 21 2780 1802
Fragments arranged by size:
3168 2780 1802 507 60 21
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2353 2487 2645
Size: 54 1425 50 275 18 523 134 158
Cuts at: 2645 3235 3428 3783 4293 4408 4997 5402 5785
Size: 590 193 355 510 115 589 405 383
Cuts at: 5785 6674 8303 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 590 589 523 510 405
383 355 275 193 158 134 115 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4408 5785 1537
Size: 275 2596 1377 4090
Fragments arranged by size:
4090 2596 1377 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2336 4329 4548 5111 6268 1353
Size: 983 1993 219 563 1157 3423
Fragments arranged by size:
3423 1993 1157 983 563 219
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2001 2782 4632 4813 6925 7299
Size: 1064 138 412 781 1850 181 2112 374
Cuts at: 7299 7445 387
Size: 146 1280
Fragments arranged by size:
2112 1850 1280 1064 781 412 374 181
146 138
EcoICRI GAG'CTC
Cuts at: 4924 5596 4924
Size: 672 7666
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3032 3041 4045 4102 4328 4604 5428
Size: 393 1400 9 1004 57 226 276 824
Cuts at: 5428 5444 6401 6998 7785 8053 1239
Size: 16 957 597 787 268 1524
Fragments arranged by size:
1524 1400 1004 957 824 787 597 393
276 268 226 57 16 9
EcoNI CCTnn'n_nnAGG
Cuts at: 3320 4553 3320
Size: 1233 7105
EcoO109I rG'GnC_Cy
Cuts at: 2148 2680 2694 2812 3596 3597 3756 3895 4433
Size: 532 14 118 784 1 159 139 538
Cuts at: 4433 4579 4623 4950 5067 5428 2148
Size: 146 44 327 117 361 5058
Fragments arranged by size:
5058 784 538 532 361 327 159 146
139 118 117 44 14 1
EcoRI G'AATT_C
Cuts at: 5647 6025 5647
Size: 378 7960
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4075 4342 4075
Size: 267 8071
FseI GG_CCGG'CC
Cuts at: 2493 2493
Size: 8338
FspI TGC'GCA
Cuts at: 2192 6844 2192
Size: 4652 3686
FspAI rTGC'GCAy
Cuts at: 2192 2192
Size: 8338
HaeII r_GCGC'y
Cuts at: 1678 2402 2519 3314 3437 3702 7485 1678
Size: 724 117 795 123 265 3783 2531
Fragments arranged by size:
3783 2531 795 724 265 123 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3188 3220 3639 3671 3992
Size: 32 135 32 1616 32 419 32 321
Cuts at: 3992 4024 4242 4274 5141 5173 6979 7011 7053
Size: 32 218 32 867 32 1806 32 42
Cuts at: 7053 7085 1373
Size: 32 2626
Fragments arranged by size:
2626 1806 1616 867 419 321 218 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4534 5682 5928 669
Size: 988 281 2596 1148 246 3079
Fragments arranged by size:
3079 2596 1148 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8338
HpaI GTT'AAC
Cuts at: 5928 5928
Size: 8338
KasI G'GCGC_C
Cuts at: 2398 3310 2398
Size: 912 7426
KpnI G_GTAC'C
Cuts at: 5663 5663
Size: 8338
MfeI C'AATT_G
Cuts at: 5937 5937
Size: 8338
MluI A'CGCG_T
Cuts at: 8214 8214
Size: 8338
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2458 7452 7636 1305
Size: 1153 4994 184 2007
Fragments arranged by size:
4994 2007 1153 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3237 10
Size: 54 1425 343 1405 5111
Fragments arranged by size:
5111 1425 1405 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2444 2939 3155 3390 3743 3951 4223
Size: 648 1278 495 216 235 353 208 272
Cuts at: 4223 4373 4379 4469 4738 4811 4881 5306 5354
Size: 150 6 90 269 73 70 425 48
Cuts at: 5354 5396 6296 6655 6814 518
Size: 42 900 359 159 2042
Fragments arranged by size:
2042 1278 900 648 495 425 359 353
272 269 235 216 208 159 150 90
73 70 48 42 6
MspA1I CmG'CkG
Cuts at: 1606 3036 3177 3276 3354 3547 3693 5018 5754
Size: 1430 141 99 78 193 146 1325 736
Cuts at: 5754 6431 7579 7824 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1430 1325 1148 736 677 245 193
146 141 99 78
NaeI GCC'GGC
Cuts at: 2016 2491 2645 3432 2016
Size: 475 154 787 6922
Fragments arranged by size:
6922 787 475 154
NarI GG'CG_CC
Cuts at: 2399 3311 2399
Size: 912 7426
NcoI C'CATG_G
Cuts at: 513 1065 2004 4374 4428 5391 5506 513
Size: 552 939 2370 54 963 115 3345
Fragments arranged by size:
3345 2370 963 939 552 115 54
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8338
NgoMIV G'CCGG_C
Cuts at: 2014 2489 2643 3430 2014
Size: 475 154 787 6922
Fragments arranged by size:
6922 787 475 154
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8338
NotI GC'GGCC_GC
Cuts at: 5785 5785
Size: 8338
NspI r_CATG'y
Cuts at: 1132 1229 2633 2786 3133 3160 4810 5696 7241
Size: 97 1404 153 347 27 1650 886 1545
Cuts at: 7241 1132
Size: 2229
Fragments arranged by size:
2229 1650 1545 1404 886 347 153 97
27
PasI CC'CwG_GG
Cuts at: 1348 2152 2203 2731 1348
Size: 804 51 528 6955
Fragments arranged by size:
6955 804 528 51
PciI A'CATG_T
Cuts at: 7237 7237
Size: 8338
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3244 4291 4669 5333 5512 1108
Size: 2136 1047 378 664 179 3934
Fragments arranged by size:
3934 2136 1047 664 378 179
PfoI T'CCnGG_A
Cuts at: 4548 4548
Size: 8338
PmeI GTTT'AAAC
Cuts at: 5642 5642
Size: 8338
PmlI CAC'GTG
Cuts at: 2224 3241 2224
Size: 1017 7321
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2552 2584 6407 6439 7920 7952 2552
Size: 32 3823 32 1481 32 2938
Fragments arranged by size:
3823 2938 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2812 3756 3895 4623 5067 5428 2812
Size: 944 139 728 444 361 5722
Fragments arranged by size:
5722 944 728 444 361 139
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8338
PsiI TTA'TAA
Cuts at: 5908 5908
Size: 8338
PspOMI G'GGCC_C
Cuts at: 2147 3596 4432 4578 4949 2147
Size: 1449 836 146 371 5536
Fragments arranged by size:
5536 1449 836 371 146
PspXI vC'TCGA_Gb
Cuts at: 2761 2761
Size: 8338
PstI C_TGCA'G
Cuts at: 830 3359 3836 4088 4243 4425 4916 5075 5690
Size: 2529 477 252 155 182 491 159 615
Cuts at: 5690 830
Size: 3478
Fragments arranged by size:
3478 2529 615 491 477 252 182 159
155
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8338
PvuII CAG'CTG
Cuts at: 3177 3547 3693 3177
Size: 370 146 7822
Fragments arranged by size:
7822 370 146
RsrII CG'GwC_CG
Cuts at: 2278 3169 2278
Size: 891 7447
SacI G_AGCT'C
Cuts at: 4926 5598 4926
Size: 672 7666
SacII CC_GC'GG
Cuts at: 5019 5019
Size: 8338
SalI G'TCGA_C
Cuts at: 1936 4532 5680 1936
Size: 2596 1148 4594
Fragments arranged by size:
4594 2596 1148
SanDI GG'GwC_CC
Cuts at: 4623 4623
Size: 8338
SapI GCTCTTCn'nnn_
Cuts at: 5111 5111
Size: 8338
SbfI CC_TGCA'GG
Cuts at: 4243 5075 5690 4243
Size: 832 615 6891
Fragments arranged by size:
6891 832 615
ScaI AGT'ACT
Cuts at: 6586 8298 6586
Size: 1712 6626
SfcI C'TryA_G
Cuts at: 826 1045 3355 3832 4084 4239 4421 4912 5071
Size: 219 2310 477 252 155 182 491 159
Cuts at: 5071 5215 5385 5686 6821 7502 7693 826
Size: 144 170 301 1135 681 191 1471
Fragments arranged by size:
2310 1471 1135 681 491 477 301 252
219 191 182 170 159 155 144
SfoI GGC'GCC
Cuts at: 2400 3312 2400
Size: 912 7426
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8338
SgrAI Cr'CCGG_yG
Cuts at: 1700 2014 1700
Size: 314 8024
SmaI CCC'GGG
Cuts at: 1872 2404 3450 4484 5665 1872
Size: 532 1046 1034 1181 4545
Fragments arranged by size:
4545 1181 1046 1034 532
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2761 6442 7343 7605 820
Size: 197 926 818 3681 901 262 1553
Fragments arranged by size:
3681 1553 926 901 818 262 197
SnaBI TAC'GTA
Cuts at: 493 5125 6129 493
Size: 4632 1004 2702
Fragments arranged by size:
4632 2702 1004
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8338
SphI G_CATG'C
Cuts at: 1132 2786 3133 4810 5696 1132
Size: 1654 347 1677 886 3774
Fragments arranged by size:
3774 1677 1654 886 347
SspI AAT'ATT
Cuts at: 5 52 5211 6262 5
Size: 47 5159 1051 2081
Fragments arranged by size:
5159 2081 1051 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8338
StyI C'CwwG_G
Cuts at: 513 1065 2004 2583 2922 3603 3957 4374 4428
Size: 552 939 579 339 681 354 417 54
Cuts at: 4428 5194 5391 5506 5777 513
Size: 766 197 115 271 3074
Fragments arranged by size:
3074 939 766 681 579 552 417 354
339 271 197 115 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2665 2915 4216 5003 5484 5548 5569
Size: 1255 1085 250 1301 787 481 64 21
Cuts at: 5569 6365 6382 6535 6720 7165 325
Size: 796 17 153 185 445 1498
Fragments arranged by size:
1498 1301 1255 1085 796 787 481 445
250 185 153 64 21 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3900 3975 4392
Size: 274 80 33 51 1443 1923 75 417
Cuts at: 4392 5289 6584 8296 96
Size: 897 1295 1712 138
Fragments arranged by size:
1923 1712 1443 1295 897 417 274 138
80 75 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2644 2676 3604 3636 3657 3689 4121 4153 8298
Size: 32 928 32 21 32 432 32 4145
Cuts at: 8298 8330 2644
Size: 32 2652
Fragments arranged by size:
4145 2652 928 432 32 32 32 32
32 21
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8338
XbaI T'CTAG_A
Cuts at: 5674 5674
Size: 8338
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4018 4288 4456 5389 4018
Size: 270 168 933 6967
Fragments arranged by size:
6967 933 270 168
XhoI C'TCGA_G
Cuts at: 1943 2761 1943
Size: 818 7520
XmaI C'CCGG_G
Cuts at: 1870 2402 3448 4482 5663 1870
Size: 532 1046 1034 1181 4545
Fragments arranged by size:
4545 1181 1046 1034 532
XmnI GAAnn'nnTTC
Cuts at: 6467 6467
Size: 8338
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6146 276
Size: 53 83 186 936 4612 2468
Fragments arranged by size:
4612 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BfrBI BplI BsiWI BstZ17I Bsu36I
EcoRV NruI NsiI PacI PsrI SexAI SfiI SrfI
SwaI