Restriction map for
pFN21AB7287
(Circular) (Six-base) MAPSORT of: pFN21AB7287.seq Check: 6062 from: 1 to: 8458
pFN21AB7287 8458 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2321 3204 2321
Size: 883 7575
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6268 278
Size: 53 83 186 936 4732 2468
Fragments arranged by size:
4732 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4632 5801 1937
Size: 2695 1169 4594
Fragments arranged by size:
4594 2695 1169
Acc65I G'GTAC_C
Cuts at: 5779 5779
Size: 8458
AclI AA'CG_TT
Cuts at: 1563 6585 6958 1563
Size: 5022 373 3063
Fragments arranged by size:
5022 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3819 3882 4595 5444 5751 6521 7905 8173
Size: 2187 63 713 849 307 770 1384 268
Cuts at: 8173 1632
Size: 1917
Fragments arranged by size:
2187 1917 1384 849 770 713 307 268
63
AfeI AGC'GCT
Cuts at: 2150 2519 2150
Size: 369 8089
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8261
AflIII A'CryG_T
Cuts at: 1203 2721 7357 8334 1203
Size: 1518 4636 977 1327
Fragments arranged by size:
4636 1518 1327 977
AgeI A'CCGG_T
Cuts at: 7289 7289
Size: 8458
AhdI GACnn_n'nnGTC
Cuts at: 7187 7187
Size: 8458
AleI CACnn'nnGTG
Cuts at: 1166 2753 3447 4523 1166
Size: 1587 694 1076 5101
Fragments arranged by size:
5101 1587 1076 694
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2921 1184
Size: 1737 6721
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4121 4153 4121
Size: 32 8426
AlwNI CAG_nnn'CTG
Cuts at: 2110 2313 2776 3307 4759 5717 6201 7773 2110
Size: 203 463 531 1452 958 484 1572 2795
Fragments arranged by size:
2795 1572 1452 958 531 484 463 203
ApaI G_GGCC'C
Cuts at: 2291 3040 2291
Size: 749 7709
ApaLI G'TGCA_C
Cuts at: 6515 7671 6515
Size: 1156 7302
ApoI r'AATT_y
Cuts at: 1493 3728 3937 5767 5993 6145 8254 1493
Size: 2235 209 1830 226 152 2109 1697
Fragments arranged by size:
2235 2109 1830 1697 226 209 152
AseI AT'TA_AT
Cuts at: 160 1032 4338 4389 7012 160
Size: 872 3306 51 2623 1606
Fragments arranged by size:
3306 2623 1606 872 51
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8458
AvaI C'yCGr_G
Cuts at: 1870 1943 2197 2290 2493 5783 1870
Size: 73 254 93 203 3290 4545
Fragments arranged by size:
4545 3290 254 203 93 73
BamHI G'GATC_C
Cuts at: 3866 5391 5788 6155 3866
Size: 1525 397 367 6169
Fragments arranged by size:
6169 1525 397 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2163 2318 2897 4400 5735
Size: 325 217 627 376 155 579 1503 1335
Cuts at: 5735 5779 7234 618
Size: 44 1455 1842
Fragments arranged by size:
1842 1503 1455 1335 627 579 376 325
217 155 44
BanII G_rGCy'C
Cuts at: 1391 2013 2291 2802 3040 3330 3426 3827 3878
Size: 622 278 511 238 290 96 401 51
Cuts at: 3878 5725 5777 1391
Size: 1847 52 4072
Fragments arranged by size:
4072 1847 622 511 401 290 278 238
96 52 51
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2320 4507 5082 5172 5563 928
Size: 971 421 2187 575 90 391 3823
Fragments arranged by size:
3823 2187 971 575 421 391 90
BbvCI CC'TCA_GC
Cuts at: 4471 4832 4471
Size: 361 8097
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3314 3348 6649 6683 1903
Size: 34 1377 34 3301 34 3678
Fragments arranged by size:
3678 3301 1377 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6350 7560 6350
Size: 1210 7248
BclI T'GATC_A
Cuts at: 1548 3162 3471 4593 1548
Size: 1614 309 1122 5413
Fragments arranged by size:
5413 1614 1122 309
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2034 2107 2885
Size: 107 122 71 631 605 362 73 778
Cuts at: 2885 7069 136
Size: 4184 1525
Fragments arranged by size:
4184 1525 778 631 605 362 122 107
73 71
BglII A'GATC_T
Cuts at: 5460 8453 5460
Size: 2993 5465
BlpI GC'TnA_GC
Cuts at: 5875 5875
Size: 8458
Bme1580I G_kGCm'C
Cuts at: 1790 2166 2291 2410 2900 3040 6519 7675 1790
Size: 376 125 119 490 140 3479 1156 2573
Fragments arranged by size:
3479 2573 1156 490 376 140 125 119
BmgBI CAC'GTC
Cuts at: 1318 2572 1318
Size: 1254 7204
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6189 7147 442
Size: 416 53 83 399 24 4772 958 1753
Fragments arranged by size:
4772 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8458
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3077 3113 3651 3892 4081 4124 4781 5739
Size: 1838 36 538 241 189 43 657 958
Cuts at: 5739 7118 1239
Size: 1379 2579
Fragments arranged by size:
2579 1838 1379 958 657 538 241 189
43 36
Bpu10I CC'TnA_GC
Cuts at: 3300 3420 4471 4832 3300
Size: 120 1051 361 6926
Fragments arranged by size:
6926 1051 361 120
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2621 2626 6583 7448 7746 2621
Size: 5 3957 865 298 3333
Fragments arranged by size:
3957 3333 865 298 5
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3467 4337 4619 5402 7121 882
Size: 617 1968 870 282 783 1719 2219
Fragments arranged by size:
2219 1968 1719 870 783 617 282
BsaAI yAC'GTr
Cuts at: 493 1204 2724 2824 6249 493
Size: 711 1520 100 3425 2702
Fragments arranged by size:
3425 2702 1520 711 100
BsaBI GATnn'nnATC
Cuts at: 3221 3530 4604 5273 8452 3221
Size: 309 1074 669 3179 3227
Fragments arranged by size:
3227 3179 1074 669 309
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2066 6265 6647 7282
Size: 53 83 186 936 533 4199 382 635
Cuts at: 7282 275
Size: 1451
Fragments arranged by size:
4199 1451 936 635 533 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3834 4317 4857 5265 6891 7289 7563 7710 8121
Size: 483 540 408 1626 398 274 147 411
Cuts at: 8121 3834
Size: 4171
Fragments arranged by size:
4171 1626 540 483 411 408 398 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2338 2368 2699 2729 3829
Size: 30 165 30 935 30 331 30 1100
Cuts at: 3829 3859 4097 4127 1178
Size: 30 238 30 5509
Fragments arranged by size:
5509 1100 935 331 238 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3817 4823 4931 5420 1186
Size: 2631 1006 108 489 4224
Fragments arranged by size:
4224 2631 1006 489 108
BseYI C'CCAG_C
Cuts at: 2284 2650 3039 4871 5702 7661 8157 2284
Size: 366 389 1832 831 1959 496 2585
Fragments arranged by size:
2585 1959 1832 831 496 389 366
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2378 4241 4566 1163
Size: 573 642 1863 325 5055
Fragments arranged by size:
5055 1863 642 573 325
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2076 2102 5908 6669 7697 1540
Size: 275 186 75 26 3806 761 1028 2301
Fragments arranged by size:
3806 2301 1028 761 275 186 75 26
BsiHKAI G_wGCw'C
Cuts at: 2013 2829 3138 3195 3330 3827 4110 5191 6519
Size: 816 309 57 135 497 283 1081 1328
Cuts at: 6519 6604 7675 2013
Size: 85 1071 2796
Fragments arranged by size:
2796 1328 1081 1071 816 497 309 283
135 85 57
BsmI GAATG_Cn'
Cuts at: 5027 5242 5969 6062 5027
Size: 215 727 93 7423
Fragments arranged by size:
7423 727 215 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2166 2291 2410 2802 2829 2900
Size: 399 223 153 125 119 392 27 71
Cuts at: 2900 3040 3138 3195 3330 3426 3827 3878 4110
Size: 140 98 57 135 96 401 51 232
Cuts at: 4110 5191 5725 5777 6519 6604 7675 1391
Size: 1081 534 52 742 85 1071 2174
Fragments arranged by size:
2174 1081 1071 742 534 401 399 392
232 223 153 140 135 125 119 98
96 85 71 57 52 51 27
BspEI T'CCGG_A
Cuts at: 4317 4857 5265 8121 4317
Size: 540 408 2856 4654
Fragments arranged by size:
4654 2856 540 408
BspHI T'CATG_A
Cuts at: 4596 6347 8077 4596
Size: 1751 1730 4977
Fragments arranged by size:
4977 1751 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2321 2736 2902 3204 5813 844
Size: 1477 415 166 302 2609 3489
Fragments arranged by size:
3489 2609 1477 415 302 166
BsrBI CCG'CTC
Cuts at: 1394 1823 2349 2635 2869 3118 6345 1394
Size: 429 526 286 234 249 3227 3507
Fragments arranged by size:
3507 3227 526 429 286 249 234
BsrDI GCAATG_nn'
Cuts at: 65 1248 5046 5165 6953 7127 65
Size: 1183 3798 119 1788 174 1396
Fragments arranged by size:
3798 1788 1396 1183 174 119
BsrFI r'CCGG_y
Cuts at: 1700 1810 2075 2663 2888 5682 7102 7289 1700
Size: 110 265 588 225 2794 1420 187 2869
Fragments arranged by size:
2869 2794 1420 588 265 225 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8458
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8458
BssSI C'ACGA_G
Cuts at: 3122 6518 7530 3122
Size: 3396 1012 4050
Fragments arranged by size:
4050 3396 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2110 794
Size: 1316 7142
BstBI TT'CG_AA
Cuts at: 7262 7262
Size: 8458
BstEII G'GTnAC_C
Cuts at: 1184 2746 4537 1184
Size: 1562 1791 5105
Fragments arranged by size:
5105 1791 1562
BstXI CCAn_nnnn'nTGG
Cuts at: 4879 4879
Size: 8458
BstYI r'GATC_y
Cuts at: 1972 2772 3216 3866 5391 5460 5788 6155 6540
Size: 800 444 650 1525 69 328 367 385
Cuts at: 6540 6557 8009 8095 8107 8453 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1525 1452 800 650 444 385 367
346 328 86 69 17 12
Bsu36I CC'TnA_GG
Cuts at: 3734 3774 3996 3734
Size: 40 222 8196
Fragments arranged by size:
8196 222 40
BtgI C'CryG_G
Cuts at: 513 1065 2004 2625 3709 4533 4747 5281 5583
Size: 552 939 621 1084 824 214 534 302
Cuts at: 5583 513
Size: 3388
Fragments arranged by size:
3388 1084 939 824 621 552 534 302
214
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3212 487
Size: 683 56 1986 5733
Fragments arranged by size:
5733 1986 683 56
BtsI GCAGTG_nn'
Cuts at: 2359 2985 3364 3442 4590 4706 5974 6768 6788
Size: 626 379 78 1148 116 1268 794 20
Cuts at: 6788 2359
Size: 4029
Fragments arranged by size:
4029 1268 1148 794 626 379 116 78
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8458
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4654 4689 529
Size: 35 4090 35 4298
Fragments arranged by size:
4298 4090 35 35
DraI TTT'AAA
Cuts at: 4090 5762 4090
Size: 1672 6786
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5695 7465 809
Size: 507 4379 1770 1802
Fragments arranged by size:
4379 1802 1770 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2073 2099 2757
Size: 54 1425 50 275 18 243 26 658
Cuts at: 2757 2886 4530 4719 5127 5386 5580 5905 6794
Size: 129 1644 189 408 259 194 325 889
Cuts at: 6794 8423 8
Size: 1629 43
Fragments arranged by size:
1644 1629 1425 889 658 408 325 275
259 243 194 189 129 54 50 43
26 18
EagI C'GGCC_G
Cuts at: 1537 1812 2073 2099 5905 1537
Size: 275 261 26 3806 4090
Fragments arranged by size:
4090 3806 275 261 26
EarI CTCTTCn'nnn_
Cuts at: 1353 2834 2843 3696 4051 5515 6388 1353
Size: 1481 9 853 355 1464 873 3423
Fragments arranged by size:
3423 1481 1464 873 853 355 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5701 7045 7419 7565 387
Size: 1064 138 4112 1344 374 146 1280
Fragments arranged by size:
4112 1344 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2011 3328 3825 2011
Size: 1317 497 6644
Fragments arranged by size:
6644 1317 497
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3077 3113 3651 3819 3882 3892 4081
Size: 393 1445 36 538 168 63 10 189
Cuts at: 4081 4124 4595 4781 5444 5739 5751 6521 7118
Size: 43 471 186 663 295 12 770 597
Cuts at: 7118 7905 8173 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1445 787 770 663 597 538 471
393 295 268 189 186 168 63 43
36 12 10
EcoNI CCTnn'n_nnAGG
Cuts at: 4898 4898
Size: 8458
EcoRI G'AATT_C
Cuts at: 3728 5767 6145 3728
Size: 2039 378 6041
Fragments arranged by size:
6041 2039 378
EcoRV GAT'ATC
Cuts at: 3805 4489 3805
Size: 684 7774
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4165 4165
Size: 8458
FseI GG_CCGG'CC
Cuts at: 2892 2892
Size: 8458
FspI TGC'GCA
Cuts at: 2477 6964 2477
Size: 4487 3971
HaeII r_GCGC'y
Cuts at: 1678 2094 2152 2270 2521 3046 5313 7605 1678
Size: 416 58 118 251 525 2267 2292 2531
Fragments arranged by size:
2531 2292 2267 525 416 251 118 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2699 2731 3578 3610 4050
Size: 32 135 32 1127 32 847 32 440
Cuts at: 4050 4082 4478 4510 4973 5005 5445 5477 7099
Size: 32 396 32 463 32 440 32 1622
Cuts at: 7099 7131 7173 7205 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1622 1127 847 463 440 440 396
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3517 5802 6048 669
Size: 988 281 1579 2285 246 3079
Fragments arranged by size:
3079 2285 1579 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3048 3972 748
Size: 2300 924 5234
Fragments arranged by size:
5234 2300 924
HpaI GTT'AAC
Cuts at: 6048 6048
Size: 8458
KpnI G_GTAC'C
Cuts at: 5783 5783
Size: 8458
MfeI C'AATT_G
Cuts at: 6057 6057
Size: 8458
MluI A'CGCG_T
Cuts at: 8334 8334
Size: 8458
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4313 5551 7572 7756 1305
Size: 3008 1238 2021 184 2007
Fragments arranged by size:
3008 2021 2007 1238 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2759 4532 4721 10
Size: 54 1425 343 927 1773 189 3747
Fragments arranged by size:
3747 1773 1425 927 343 189 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2753 2993 3231 3447 4523 5123 5129
Size: 648 1587 240 238 216 1076 600 6
Cuts at: 5129 5184 6416 6775 6934 518
Size: 55 1232 359 159 2042
Fragments arranged by size:
2042 1587 1232 1076 648 600 359 240
238 216 159 55 6
MspA1I CmG'CkG
Cuts at: 1606 2107 2178 2184 2251 2343 2361 2367 2739
Size: 501 71 6 67 92 18 6 372
Cuts at: 2739 2863 3005 4475 4759 5585 5706 5874 6551
Size: 124 142 1470 284 826 121 168 677
Cuts at: 6551 7699 7944 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1470 1148 826 677 501 372 284
245 168 142 124 121 92 71 67
18 6 6
NaeI GCC'GGC
Cuts at: 2077 2890 2077
Size: 813 7645
NcoI C'CATG_G
Cuts at: 513 1065 2004 2625 3709 4533 4747 513
Size: 552 939 621 1084 824 214 4224
Fragments arranged by size:
4224 1084 939 824 621 552 214
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8458
NgoMIV G'CCGG_C
Cuts at: 2075 2888 2075
Size: 813 7645
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8458
NotI GC'GGCC_GC
Cuts at: 5905 5905
Size: 8458
NspI r_CATG'y
Cuts at: 1132 1229 3983 4855 5816 7361 1132
Size: 97 2754 872 961 1545 2229
Fragments arranged by size:
2754 2229 1545 961 872 97
PacI TTA_AT'TAA
Cuts at: 3765 3765
Size: 8458
PasI CC'CwG_GG
Cuts at: 1348 3641 1348
Size: 2293 6165
PciI A'CATG_T
Cuts at: 7357 7357
Size: 8458
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2776 2968 5017 1108
Size: 1668 192 2049 4549
Fragments arranged by size:
4549 2049 1668 192
PfoI T'CCnGG_A
Cuts at: 2331 3054 3153 3869 2331
Size: 723 99 716 6920
Fragments arranged by size:
6920 723 716 99
PmeI GTTT'AAAC
Cuts at: 5762 5762
Size: 8458
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6527 6559 8040 8072 6527
Size: 32 1481 32 6913
Fragments arranged by size:
6913 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8458
PsiI TTA'TAA
Cuts at: 4296 6028 4296
Size: 1732 6726
PspOMI G'GGCC_C
Cuts at: 2287 3036 2287
Size: 749 7709
PstI C_TGCA'G
Cuts at: 830 2733 2913 3201 5810 830
Size: 1903 180 288 2609 3478
Fragments arranged by size:
3478 2609 1903 288 180
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8458
PvuII CAG'CTG
Cuts at: 2251 3005 4475 4759 2251
Size: 754 1470 284 5950
Fragments arranged by size:
5950 1470 754 284
SacI G_AGCT'C
Cuts at: 2013 3330 3827 2013
Size: 1317 497 6644
Fragments arranged by size:
6644 1317 497
SacII CC_GC'GG
Cuts at: 5586 5586
Size: 8458
SalI G'TCGA_C
Cuts at: 1936 5800 1936
Size: 3864 4594
SapI GCTCTTCn'nnn_
Cuts at: 2834 2843 2834
Size: 9 8449
SbfI CC_TGCA'GG
Cuts at: 5810 5810
Size: 8458
ScaI AGT'ACT
Cuts at: 4508 5573 6706 8418 4508
Size: 1065 1133 1712 4548
Fragments arranged by size:
4548 1712 1133 1065
SexAI A'CCwGG_T
Cuts at: 2961 2961
Size: 8458
SfcI C'TryA_G
Cuts at: 826 1045 2443 2729 2848 2909 3197 5806 6941
Size: 219 1398 286 119 61 288 2609 1135
Cuts at: 6941 7622 7813 826
Size: 681 191 1471
Fragments arranged by size:
2609 1471 1398 1135 681 288 286 219
191 119 61
SfiI GGCCn_nnn'nGGCC
Cuts at: 2885 2885
Size: 8458
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8458
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8458
SmaI CCC'GGG
Cuts at: 1872 2292 5785 1872
Size: 420 3493 4545
Fragments arranged by size:
4545 3493 420
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2605 2636 6562 7463 7725 820
Size: 197 926 662 31 3926 901 262 1553
Fragments arranged by size:
3926 1553 926 901 662 262 197 31
SnaBI TAC'GTA
Cuts at: 493 6249 493
Size: 5756 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8458
SphI G_CATG'C
Cuts at: 1132 5816 1132
Size: 4684 3774
SspI AAT'ATT
Cuts at: 5 52 6382 5
Size: 47 6330 2081
Fragments arranged by size:
6330 2081 47
StuI AGG'CCT
Cuts at: 1517 2787 4641 5139 1517
Size: 1270 1854 498 4836
Fragments arranged by size:
4836 1854 1270 498
StyI C'CwwG_G
Cuts at: 513 1065 2004 2625 3709 4533 4695 4747 5897
Size: 552 939 621 1084 824 162 52 1150
Cuts at: 5897 513
Size: 3074
Fragments arranged by size:
3074 1150 1084 939 824 621 552 162
52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2743 2783 4388 4920 5094 5716 6485
Size: 1255 1163 40 1605 532 174 622 769
Cuts at: 6485 6502 6655 6840 7285 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1605 1498 1255 1163 769 622 532 445
185 174 153 40 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2733 4506 4764
Size: 274 80 33 51 1443 756 1773 258
Cuts at: 4764 4795 5571 6704 8416 96
Size: 31 776 1133 1712 138
Fragments arranged by size:
1773 1712 1443 1133 776 756 274 258
138 80 51 33 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2338 2370 3829 3861 8418 8450 2338
Size: 32 1459 32 4557 32 2346
Fragments arranged by size:
4557 2346 1459 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3292 1316
Size: 1976 6482
XbaI T'CTAG_A
Cuts at: 5794 5794
Size: 8458
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2773 3088 3667 4729 2773
Size: 315 579 1062 6502
Fragments arranged by size:
6502 1062 579 315
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8458
XmaI C'CCGG_G
Cuts at: 1870 2290 5783 1870
Size: 420 3493 4545
Fragments arranged by size:
4545 3493 420
XmnI GAAnn'nnTTC
Cuts at: 3728 6587 3728
Size: 2859 5599
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6266 276
Size: 53 83 186 936 4732 2468
Fragments arranged by size:
4732 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoRI EcoRV FalI FseI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PacI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI
BstZ17I DraIII EcoO109I FspAI KasI NarI NruI NsiI
PmlI PpuMI PspXI PsrI RsrII SanDI SfoI SrfI
SwaI