Restriction map for
pFN21AB7280
(Circular) (Six-base) MAPSORT of: pFN21AB7280.seq Check: 3799 from: 1 to: 8713
pFN21AB7280 8713 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2504 5439 2504
Size: 2935 5778
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2196 3549 5787 6523
Size: 53 83 186 936 660 1353 2238 736
Cuts at: 6523 278
Size: 2468
Fragments arranged by size:
2468 2238 1353 936 736 660 186 83
53
AccI GT'mk_AC
Cuts at: 1937 4143 6056 1937
Size: 2206 1913 4594
Fragments arranged by size:
4594 2206 1913
Acc65I G'GTAC_C
Cuts at: 2247 6034 2247
Size: 3787 4926
AclI AA'CG_TT
Cuts at: 1563 6840 7213 1563
Size: 5277 373 3063
Fragments arranged by size:
5277 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2693 3038 3370 4294 4694 5765 6776 8160
Size: 1061 345 332 924 400 1071 1011 1384
Cuts at: 8160 8428 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1071 1061 1011 924 400 345
332 268
AfeI AGC'GCT
Cuts at: 3886 3886
Size: 8713
AflII C'TTAA_G
Cuts at: 820 1017 3923 820
Size: 197 2906 5610
Fragments arranged by size:
5610 2906 197
AflIII A'CryG_T
Cuts at: 1203 4146 7612 8589 1203
Size: 2943 3466 977 1327
Fragments arranged by size:
3466 2943 1327 977
AgeI A'CCGG_T
Cuts at: 7544 7544
Size: 8713
AhdI GACnn_n'nnGTC
Cuts at: 4139 7442 4139
Size: 3303 5410
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8713
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8713
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5705 5737 5705
Size: 32 8681
AlwNI CAG_nnn'CTG
Cuts at: 2957 5632 5732 6456 8028 2957
Size: 2675 100 724 1572 3642
Fragments arranged by size:
3642 2675 1572 724 100
ApaI G_GGCC'C
Cuts at: 2318 3053 3345 3372 3648 3801 3810 3864 4032
Size: 735 292 27 276 153 9 54 168
Cuts at: 4032 4337 4545 4637 4673 4952 4980 5036 5046
Size: 305 208 92 36 279 28 56 10
Cuts at: 5046 5055 2318
Size: 9 5976
Fragments arranged by size:
5976 735 305 292 279 276 208 168
153 92 56 54 36 28 27 10
9 9
ApaLI G'TGCA_C
Cuts at: 5123 5426 6770 7926 5123
Size: 303 1344 1156 5910
Fragments arranged by size:
5910 1344 1156 303
ApoI r'AATT_y
Cuts at: 1493 6022 6248 6400 8509 1493
Size: 4529 226 152 2109 1697
Fragments arranged by size:
4529 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7267 160
Size: 872 6235 1606
Fragments arranged by size:
6235 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8713
AvaI C'yCGr_G
Cuts at: 1870 1943 2081 2768 2778 3100 3278 3462 3596
Size: 73 138 687 10 322 178 184 134
Cuts at: 3596 3613 3989 4129 4270 4381 4445 4604 4784
Size: 17 376 140 141 111 64 159 180
Cuts at: 4784 5135 5381 5750 6038 1870
Size: 351 246 369 288 4545
Fragments arranged by size:
4545 687 376 369 351 322 288 246
184 180 178 159 141 140 138 134
111 73 64 17 10
BamHI G'GATC_C
Cuts at: 3077 3931 4163 6043 6410 3077
Size: 854 232 1880 367 5380
Fragments arranged by size:
5380 1880 854 367 232
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2016 2043 2247 2397
Size: 325 217 627 221 8 27 204 150
Cuts at: 2397 2603 3200 3689 4325 4652 4941 5069 5594
Size: 206 597 489 636 327 289 128 525
Cuts at: 5594 5793 6034 7489 618
Size: 199 241 1455 1842
Fragments arranged by size:
1842 1455 636 627 597 525 489 327
325 289 241 221 217 206 204 199
150 128 27 8
BanII G_rGCy'C
Cuts at: 1391 2318 2613 2702 2769 2935 3053 3345 3372
Size: 927 295 89 67 166 118 292 27
Cuts at: 3372 3648 3721 3766 3801 3810 3864 4032 4250
Size: 276 73 45 35 9 54 168 218
Cuts at: 4250 4337 4545 4637 4673 4785 4952 4980 5036
Size: 87 208 92 36 112 167 28 56
Cuts at: 5036 5046 5055 5175 5388 5407 6032 1391
Size: 10 9 120 213 19 625 4072
Fragments arranged by size:
4072 927 625 295 292 276 218 213
208 168 167 166 120 118 112 92
89 87 73 67 56 54 45 36
35 28 27 19 10 9 9
BbeI G_GCGC'C
Cuts at: 2012 2047 2607 3693 4656 4945 5073 2012
Size: 35 560 1086 963 289 128 5652
Fragments arranged by size:
5652 1086 963 560 289 128 35
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3246 928
Size: 971 1347 6395
Fragments arranged by size:
6395 1347 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2749 2783 4307 4341 6904 6938 1903
Size: 34 812 34 1524 34 2563 34 3678
Fragments arranged by size:
3678 2563 1524 812 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6605 7815 6605
Size: 1210 7503
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8713
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2038 3057 3066
Size: 107 122 71 631 605 366 1019 9
Cuts at: 3066 3367 3510 3805 4008 4036 4113 4320 4818
Size: 301 143 295 203 28 77 207 498
Cuts at: 4818 4827 4836 4845 4896 4956 4965 5031 5050
Size: 9 9 9 51 60 9 66 19
Cuts at: 5050 7324 136
Size: 2274 1525
Fragments arranged by size:
2274 1525 1019 631 605 498 366 301
295 207 203 143 122 107 77 71
66 60 51 28 19 9 9 9
9 9
BglII A'GATC_T
Cuts at: 8708 8708
Size: 8713
BlpI GC'TnA_GC
Cuts at: 2667 6130 2667
Size: 3463 5250
Bme1580I G_kGCm'C
Cuts at: 1790 2021 2318 2400 3053 3116 3345 3372 3648
Size: 231 297 82 653 63 229 27 276
Cuts at: 3648 3801 3810 3864 4032 4330 4337 4545 4637
Size: 153 9 54 168 298 7 208 92
Cuts at: 4637 4673 4952 4980 5036 5046 5055 5127 5430
Size: 36 279 28 56 10 9 72 303
Cuts at: 5430 5597 5673 5758 6774 7930 1790
Size: 167 76 85 1016 1156 2573
Fragments arranged by size:
2573 1156 1016 653 303 298 297 279
276 231 229 208 168 167 153 92
85 82 76 72 63 56 54 36
28 27 10 9 9 7
BmgBI CAC'GTC
Cuts at: 1318 2056 5874 1318
Size: 738 3818 4157
Fragments arranged by size:
4157 3818 738
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3110 3669 6444
Size: 416 53 83 399 24 1693 559 2775
Cuts at: 6444 7402 442
Size: 958 1753
Fragments arranged by size:
2775 1753 1693 958 559 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8713
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5908 5908
Size: 8713
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2609 2615 2883 3270 3348 3464 4521 4539
Size: 1370 6 268 387 78 116 1057 18
Cuts at: 4539 4596 4623 4792 5234 7373 1239
Size: 57 27 169 442 2139 2579
Fragments arranged by size:
2579 2139 1370 1057 442 387 268 169
116 78 57 27 18 6
Bpu10I CC'TnA_GC
Cuts at: 2622 4172 2622
Size: 1550 7163
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5669 6838 7703 8001 5669
Size: 1169 865 298 6381
Fragments arranged by size:
6381 1169 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3110 3312 4737 7376 882
Size: 617 1611 202 1425 2639 2219
Fragments arranged by size:
2639 2219 1611 1425 617 202
BsaAI yAC'GTr
Cuts at: 493 1204 2536 2967 4147 4844 5425 6504 493
Size: 711 1332 431 1180 697 581 1079 2702
Fragments arranged by size:
2702 1332 1180 1079 711 697 581 431
BsaBI GATnn'nnATC
Cuts at: 8707 8707
Size: 8713
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2044 2193 2604
Size: 53 83 186 936 476 35 149 411
Cuts at: 2604 2775 3546 3690 4653 4942 5070 5784 6520
Size: 171 771 144 963 289 128 714 736
Cuts at: 6520 6902 7537 275
Size: 382 635 1451
Fragments arranged by size:
1451 963 936 771 736 714 635 476
411 382 289 186 171 149 144 128
83 53 35
BsaWI w'CCGG_w
Cuts at: 2747 2982 5199 7146 7544 7818 7965 8376 2747
Size: 235 2217 1947 398 274 147 411 3084
Fragments arranged by size:
3084 2217 1947 411 398 274 235 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2808 2838 3807 3837 5402
Size: 30 165 30 1405 30 969 30 1565
Cuts at: 5402 5432 5705 5735 5872 5902 1178
Size: 30 273 30 137 30 3989
Fragments arranged by size:
3989 1565 1405 969 273 165 137 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2138 2561 2810 2918 5065 5183 5902 1186
Size: 952 423 249 108 2147 118 719 3997
Fragments arranged by size:
3997 2147 952 719 423 249 118 108
BseYI C'CCAG_C
Cuts at: 2163 2258 2365 3142 4025 5113 5388 5705 5709
Size: 95 107 777 883 1088 275 317 4
Cuts at: 5709 5920 7916 8412 2163
Size: 211 1996 496 2464
Fragments arranged by size:
2464 1996 1088 883 777 496 317 275
211 107 95 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2408 2454 5207 5270 5300 1163
Size: 573 672 46 2753 63 30 4576
Fragments arranged by size:
4576 2753 672 573 63 46 30
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4199 4400 4436 5438 5659 6163
Size: 275 186 2198 201 36 1002 221 504
Cuts at: 6163 6924 7952 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 2198 1028 1002 761 504 275 221
201 186 36
BsiHKAI G_wGCw'C
Cuts at: 2061 2935 3739 5127 5175 5319 5430 5493 5979
Size: 874 804 1388 48 144 111 63 486
Cuts at: 5979 6774 6859 7930 2061
Size: 795 85 1071 2844
Fragments arranged by size:
2844 1388 1071 874 804 795 486 144
111 85 63 48
BsmI GAATG_Cn'
Cuts at: 2565 4521 6224 6317 2565
Size: 1956 1703 93 4961
Fragments arranged by size:
4961 1956 1703 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2792 5864 2792
Size: 3072 5641
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2061 2318 2400 2613 2702 2769
Size: 399 231 40 257 82 213 89 67
Cuts at: 2769 2935 3053 3116 3345 3372 3648 3721 3739
Size: 166 118 63 229 27 276 73 18
Cuts at: 3739 3766 3801 3810 3864 4032 4250 4330 4337
Size: 27 35 9 54 168 218 80 7
Cuts at: 4337 4545 4637 4673 4785 4952 4980 5036 5046
Size: 208 92 36 112 167 28 56 10
Cuts at: 5046 5055 5127 5175 5319 5388 5407 5430 5493
Size: 9 72 48 144 69 19 23 63
Cuts at: 5493 5597 5673 5758 5979 6032 6774 6859 7930
Size: 104 76 85 221 53 742 85 1071
Cuts at: 7930 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 276 257 231 229
221 218 213 208 168 167 166 144
118 112 104 92 89 85 85 82
80 76 73 72 69 67 63 63
56 54 53 48 40 36 35 28
27 27 23 19 18 10 9 9
7
BspEI T'CCGG_A
Cuts at: 2747 2982 5199 8376 2747
Size: 235 2217 3177 3084
Fragments arranged by size:
3177 3084 2217 235
BspHI T'CATG_A
Cuts at: 5997 6602 8332 5997
Size: 605 1730 6378
Fragments arranged by size:
6378 1730 605
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2158 2504 3781 4231 5439 6068 844
Size: 1314 346 1277 450 1208 629 3489
Fragments arranged by size:
3489 1314 1277 1208 629 450 346
BsrBI CCG'CTC
Cuts at: 1394 1823 2636 3750 6600 1394
Size: 429 813 1114 2850 3507
Fragments arranged by size:
3507 2850 1114 813 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4020 4585 7208 7382 65
Size: 1183 2772 565 2623 174 1396
Fragments arranged by size:
2772 2623 1396 1183 565 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3560 4194 5437 5485 7357 7544 1700
Size: 110 1750 634 1243 48 1872 187 2869
Fragments arranged by size:
2869 1872 1750 1243 634 187 110 48
BsrGI T'GTAC_A
Cuts at: 96 5634 96
Size: 5538 3175
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8713
BssSI C'ACGA_G
Cuts at: 3738 5126 5235 6773 7785 3738
Size: 1388 109 1538 1012 4666
Fragments arranged by size:
4666 1538 1388 1012 109
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8713
BstBI TT'CG_AA
Cuts at: 7517 7517
Size: 8713
BstEII G'GTnAC_C
Cuts at: 1184 2170 1184
Size: 986 7727
BstYI r'GATC_y
Cuts at: 1972 3077 3422 3931 4075 4163 6043 6410 6795
Size: 1105 345 509 144 88 1880 367 385
Cuts at: 6795 6812 8264 8350 8362 8708 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1880 1452 1105 509 385 367 346
345 144 88 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4188 4911 5077 5789 4188
Size: 723 166 712 7112
Fragments arranged by size:
7112 723 712 166
BtgI C'CryG_G
Cuts at: 513 1065 2004 2027 2033 2100 2355 2913 5064
Size: 552 939 23 6 67 255 558 2151
Cuts at: 5064 5274 5306 5542 5580 5898 513
Size: 210 32 236 38 318 3328
Fragments arranged by size:
3328 2151 939 558 552 318 255 236
210 67 38 32 23 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2742 487
Size: 683 56 1516 6458
Fragments arranged by size:
6458 1516 683 56
BtsI GCAGTG_nn'
Cuts at: 2282 2707 6229 7023 7043 2282
Size: 425 3522 794 20 3952
Fragments arranged by size:
3952 3522 794 425 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8713
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8678
DraI TTT'AAA
Cuts at: 6017 6017
Size: 8713
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2192 2978 4166 4383 4437 7720 809
Size: 507 876 786 1188 217 54 3283 1802
Fragments arranged by size:
3283 1802 1188 876 786 507 217 54
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2024 2030 2233
Size: 54 1425 50 275 18 194 6 203
Cuts at: 2233 2300 2482 3058 4196 4397 4433 5157 5256
Size: 67 182 576 1138 201 36 724 99
Cuts at: 5256 5361 5435 5895 6160 7049 8678 8
Size: 105 74 460 265 889 1629 43
Fragments arranged by size:
1629 1425 1138 889 724 576 460 275
265 203 201 194 182 105 99 74
67 54 50 43 36 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 4196 4397 4433 5435 6160 1537
Size: 275 2384 201 36 1002 725 4090
Fragments arranged by size:
4090 2384 1002 725 275 201 36
EarI CTCTTCn'nnn_
Cuts at: 1353 2270 2618 3631 5807 6643 1353
Size: 917 348 1013 2176 836 3423
Fragments arranged by size:
3423 2176 1013 917 836 348
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5893 7300 7674 7820 387
Size: 1064 138 4304 1407 374 146 1280
Fragments arranged by size:
4304 1407 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2933 5173 2933
Size: 2240 6473
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2609 2615 2693 2883 3038 3270 3348
Size: 393 977 6 78 190 155 232 78
Cuts at: 3348 3370 3464 4294 4521 4539 4596 4623 4694
Size: 22 94 830 227 18 57 27 71
Cuts at: 4694 4792 5234 5765 6776 7373 8160 8428 1239
Size: 98 442 531 1011 597 787 268 1524
Fragments arranged by size:
1524 1011 977 830 787 597 531 442
393 268 232 227 190 155 98 94
78 78 71 57 27 22 18 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2717 2717
Size: 8713
EcoO109I rG'GnC_Cy
Cuts at: 2152 2314 2584 2686 3068 3131 3173 3182 3299
Size: 162 270 102 382 63 42 9 117
Cuts at: 3299 3341 3342 3350 3359 3368 3369 3377 3448
Size: 42 1 8 9 9 1 8 71
Cuts at: 3448 3458 3554 3590 3617 3626 3644 3645 3788
Size: 10 96 36 27 9 18 1 143
Cuts at: 3788 3798 3807 3860 3861 3902 4029 4133 4184
Size: 10 9 53 1 41 127 104 51
Cuts at: 4184 4229 4274 4334 4376 4532 4541 4542 4550
Size: 45 45 60 42 156 9 1 8
Cuts at: 4550 4633 4634 4643 4670 4865 4916 4948 4949
Size: 83 1 9 27 195 51 32 1
Cuts at: 4949 4967 4976 4977 5033 5043 5052 5629 2152
Size: 18 9 1 56 10 9 577 5236
Fragments arranged by size:
5236 577 382 270 195 162 156 143
127 117 104 102 96 83 71 63
60 56 53 51 51 45 45 42
42 42 41 36 32 27 27 18
18 10 10 10 9 9 9 9
9 9 9 9 9 8 8 8
1 1 1 1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 6022 6400 6022
Size: 378 8335
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3421 3421
Size: 8713
FseI GG_CCGG'CC
Cuts at: 4198 4198
Size: 8713
FspI TGC'GCA
Cuts at: 2681 7219 2681
Size: 4538 4175
HaeII r_GCGC'y
Cuts at: 1678 2012 2047 2607 3693 3888 4656 4945 5073
Size: 334 35 560 1086 195 768 289 128
Cuts at: 5073 7860 1678
Size: 2787 2531
Fragments arranged by size:
2787 2531 1086 768 560 334 289 195
128 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2729 2761 2945 2977 3807
Size: 32 135 32 1157 32 184 32 830
Cuts at: 3807 3839 4125 4157 4849 4866 4881 4898 5872
Size: 32 286 32 692 17 15 17 974
Cuts at: 5872 5904 5944 5976 7354 7386 7428 7460 1373
Size: 32 40 32 1378 32 42 32 2626
Fragments arranged by size:
2626 1378 1157 974 830 692 286 184
135 42 40 32 32 32 32 32
32 32 32 32 32 17 17 15
HincII GTy'rAC
Cuts at: 669 1657 1938 3873 5680 6057 6303 669
Size: 988 281 1935 1807 377 246 3079
Fragments arranged by size:
3079 1935 1807 988 377 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8713
HpaI GTT'AAC
Cuts at: 6303 6303
Size: 8713
KasI G'GCGC_C
Cuts at: 2008 2043 2603 3689 4652 4941 5069 2008
Size: 35 560 1086 963 289 128 5652
Fragments arranged by size:
5652 1086 963 560 289 128 35
KpnI G_GTAC'C
Cuts at: 2251 6038 2251
Size: 3787 4926
MfeI C'AATT_G
Cuts at: 6312 6312
Size: 8713
MluI A'CGCG_T
Cuts at: 8589 8589
Size: 8713
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3310 3567 3952 4131 4695 7827 8011 1305
Size: 2005 257 385 179 564 3132 184 2007
Fragments arranged by size:
3132 2007 2005 564 385 257 184 179
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2026 2235 5363 10
Size: 54 1425 343 194 209 3128 3360
Fragments arranged by size:
3360 3128 1425 343 209 194 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3413 5153 6671 7030 7189 518
Size: 648 2247 1740 1518 359 159 2042
Fragments arranged by size:
2247 2042 1740 1518 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2035 2102 2763 3830 5212 5284 5308 5544
Size: 429 67 661 1067 1382 72 24 236
Cuts at: 5544 5709 5743 6129 6806 7954 8199 1606
Size: 165 34 386 677 1148 245 2120
Fragments arranged by size:
2120 1382 1148 1067 677 661 429 386
245 236 165 72 67 34 24
NaeI GCC'GGC
Cuts at: 3562 4196 5439 3562
Size: 634 1243 6836
Fragments arranged by size:
6836 1243 634
NarI GG'CG_CC
Cuts at: 2009 2044 2604 3690 4653 4942 5070 2009
Size: 35 560 1086 963 289 128 5652
Fragments arranged by size:
5652 1086 963 560 289 128 35
NcoI C'CATG_G
Cuts at: 513 1065 2004 2027 2355 5064 513
Size: 552 939 23 328 2709 4162
Fragments arranged by size:
4162 2709 939 552 328 23
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8713
NgoMIV G'CCGG_C
Cuts at: 3560 4194 5437 3560
Size: 634 1243 6836
Fragments arranged by size:
6836 1243 634
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8713
NotI GC'GGCC_GC
Cuts at: 6160 6160
Size: 8713
NspI r_CATG'y
Cuts at: 1132 1229 5533 6071 7616 1132
Size: 97 4304 538 1545 2229
Fragments arranged by size:
4304 2229 1545 538 97
PasI CC'CwG_GG
Cuts at: 1348 2318 2990 3127 3179 3323 3794 3803 4033
Size: 970 672 137 52 144 471 9 230
Cuts at: 4033 4330 4853 4973 5039 5048 1348
Size: 297 523 120 66 9 5013
Fragments arranged by size:
5013 970 672 523 471 297 230 144
137 120 66 52 9 9
PciI A'CATG_T
Cuts at: 7612 7612
Size: 8713
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8713
PfoI T'CCnGG_A
Cuts at: 2049 3481 3631 4216 4426 4612 4639 2049
Size: 1432 150 585 210 186 27 6123
Fragments arranged by size:
6123 1432 585 210 186 150 27
PmeI GTTT'AAAC
Cuts at: 6017 6017
Size: 8713
PmlI CAC'GTG
Cuts at: 2967 4844 2967
Size: 1877 6836
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5705 5737 6782 6814 8295 8327 5705
Size: 32 1045 32 1481 32 6091
Fragments arranged by size:
6091 1481 1045 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2152 2686 3131 3173 3299 3350 3377 3458 3554
Size: 534 445 42 126 51 27 81 96
Cuts at: 3554 3590 3617 3902 4133 4184 4229 4274 4376
Size: 36 27 285 231 51 45 45 102
Cuts at: 4376 4532 4643 4865 4916 5629 2152
Size: 156 111 222 51 713 5236
Fragments arranged by size:
5236 713 534 445 285 231 222 156
126 111 102 96 81 51 51 51
45 45 42 36 27 27
PshAI GACnn'nnGTC
Cuts at: 1613 3297 1613
Size: 1684 7029
PsiI TTA'TAA
Cuts at: 6283 6283
Size: 8713
PspOMI G'GGCC_C
Cuts at: 2314 3049 3341 3368 3644 3797 3806 3860 4028
Size: 735 292 27 276 153 9 54 168
Cuts at: 4028 4333 4541 4633 4669 4948 4976 5032 5042
Size: 305 208 92 36 279 28 56 10
Cuts at: 5042 5051 2314
Size: 9 5976
Fragments arranged by size:
5976 735 305 292 279 276 208 168
153 92 56 54 36 28 27 10
9 9
PspXI vC'TCGA_Gb
Cuts at: 3462 3462
Size: 8713
PstI C_TGCA'G
Cuts at: 830 2142 3139 4228 5628 6065 830
Size: 1312 997 1089 1400 437 3478
Fragments arranged by size:
3478 1400 1312 1089 997 437
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8713
PvuII CAG'CTG
Cuts at: 5212 5284 5709 5212
Size: 72 425 8216
Fragments arranged by size:
8216 425 72
SacI G_AGCT'C
Cuts at: 2935 5175 2935
Size: 2240 6473
SacII CC_GC'GG
Cuts at: 2036 2103 5309 5545 2036
Size: 67 3206 236 5204
Fragments arranged by size:
5204 3206 236 67
SalI G'TCGA_C
Cuts at: 1936 6055 1936
Size: 4119 4594
SanDI GG'GwC_CC
Cuts at: 2152 2686 3590 3617 4274 4916 2152
Size: 534 904 27 657 642 5949
Fragments arranged by size:
5949 904 657 642 534 27
SapI GCTCTTCn'nnn_
Cuts at: 2618 5807 2618
Size: 3189 5524
SbfI CC_TGCA'GG
Cuts at: 3139 4228 5628 6065 3139
Size: 1089 1400 437 5787
Fragments arranged by size:
5787 1400 1089 437
ScaI AGT'ACT
Cuts at: 6961 8673 6961
Size: 1712 7001
SexAI A'CCwGG_T
Cuts at: 2428 3091 5496 2428
Size: 663 2405 5645
Fragments arranged by size:
5645 2405 663
SfcI C'TryA_G
Cuts at: 826 1045 2138 3135 4224 5624 6061 7196 7877
Size: 219 1093 997 1089 1400 437 1135 681
Cuts at: 7877 8068 826
Size: 191 1471
Fragments arranged by size:
1471 1400 1135 1093 1089 997 681 437
219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3057 3066 3367 3805 4036 4113 4827 4836 4896
Size: 9 301 438 231 77 714 9 60
Cuts at: 4896 4956 4965 5031 5050 3057
Size: 60 9 66 19 6720
Fragments arranged by size:
6720 714 438 301 231 77 66 60
60 19 9 9 9
SfoI GGC'GCC
Cuts at: 2010 2045 2605 3691 4654 4943 5071 2010
Size: 35 560 1086 963 289 128 5652
Fragments arranged by size:
5652 1086 963 560 289 128 35
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8713
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8713
SmaI CCC'GGG
Cuts at: 1872 2780 3102 3280 3598 3615 3991 4131 4272
Size: 908 322 178 318 17 376 140 141
Cuts at: 4272 4383 4447 4606 4786 5752 6040 1872
Size: 111 64 159 180 966 288 4545
Fragments arranged by size:
4545 966 908 376 322 318 288 180
178 159 141 140 111 64 17
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3462 3923 5684 6817 7718 7980
Size: 197 926 1519 461 1761 1133 901 262
Cuts at: 7980 820
Size: 1553
Fragments arranged by size:
1761 1553 1519 1133 926 901 461 262
197
SnaBI TAC'GTA
Cuts at: 493 6504 493
Size: 6011 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8713
SphI G_CATG'C
Cuts at: 1132 5533 6071 1132
Size: 4401 538 3774
Fragments arranged by size:
4401 3774 538
SspI AAT'ATT
Cuts at: 5 52 6637 5
Size: 47 6585 2081
Fragments arranged by size:
6585 2081 47
StuI AGG'CCT
Cuts at: 1517 3504 5909 1517
Size: 1987 2405 4321
Fragments arranged by size:
4321 2405 1987
StyI C'CwwG_G
Cuts at: 513 1065 2004 2027 2146 2355 2856 3303 3355
Size: 552 939 23 119 209 501 447 52
Cuts at: 3355 4233 4278 4353 4495 4647 4869 5064 6009
Size: 878 45 75 142 152 222 195 945
Cuts at: 6009 6152 513
Size: 143 3074
Fragments arranged by size:
3074 945 939 878 552 501 447 222
209 195 152 143 142 119 75 52
45 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2189 2562 3668 4761 5870 6740 6757
Size: 1255 609 373 1106 1093 1109 870 17
Cuts at: 6757 6910 7095 7540 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1109 1106 1093 870 609 445
373 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5634 6959 8671
Size: 274 80 33 51 1443 3657 1325 1712
Cuts at: 8671 96
Size: 138
Fragments arranged by size:
3657 1712 1443 1325 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2274 2306 3258 3290 5402 5434 8673 8705 2274
Size: 32 952 32 2112 32 3239 32 2282
Fragments arranged by size:
3239 2282 2112 952 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4091 1316
Size: 2775 5938
XbaI T'CTAG_A
Cuts at: 6049 6049
Size: 8713
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2362 2710 2854 4240 4663 2362
Size: 348 144 1386 423 6412
Fragments arranged by size:
6412 1386 423 348 144
XhoI C'TCGA_G
Cuts at: 1943 3462 1943
Size: 1519 7194
XmaI C'CCGG_G
Cuts at: 1870 2778 3100 3278 3596 3613 3989 4129 4270
Size: 908 322 178 318 17 376 140 141
Cuts at: 4270 4381 4445 4604 4784 5750 6038 1870
Size: 111 64 159 180 966 288 4545
Fragments arranged by size:
4545 966 908 376 322 318 288 180
178 159 141 140 111 64 17
XmnI GAAnn'nnTTC
Cuts at: 5195 5993 6842 5195
Size: 798 849 7066
Fragments arranged by size:
7066 849 798
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2194 3547 5785 6521
Size: 53 83 186 936 660 1353 2238 736
Cuts at: 6521 276
Size: 2468
Fragments arranged by size:
2468 2238 1353 936 736 660 186 83
53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbvCI BfrBI BsiWI BstXI BstZ17I
DraIII EcoRV FspAI NruI NsiI PacI PsrI RsrII
SrfI SwaI