Restriction map for
pFN21AB7276
(Circular) (Six-base) MAPSORT of: pFN21AB7276.seq Check: 4179 from: 1 to: 5851
pFN21AB7276 5851 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3661 278
Size: 53 83 186 936 2125 2468
Fragments arranged by size:
2468 2125 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3194 1937
Size: 1257 4594
Acc65I G'GTAC_C
Cuts at: 3172 3172
Size: 5851
AclI AA'CG_TT
Cuts at: 1563 3978 4351 1563
Size: 2415 373 3063
Fragments arranged by size:
3063 2415 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2438 2698 2951 3914 5298 5566 1632
Size: 806 260 253 963 1384 268 1917
Fragments arranged by size:
1917 1384 963 806 268 260 253
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5654
AflIII A'CryG_T
Cuts at: 1203 4750 5727 1203
Size: 3547 977 1327
Fragments arranged by size:
3547 1327 977
AgeI A'CCGG_T
Cuts at: 4682 4682
Size: 5851
AhdI GACnn_n'nnGTC
Cuts at: 4580 4580
Size: 5851
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5851
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5851
AlwNI CAG_nnn'CTG
Cuts at: 2356 3594 5166 2356
Size: 1238 1572 3041
Fragments arranged by size:
3041 1572 1238
ApaLI G'TGCA_C
Cuts at: 3908 5064 3908
Size: 1156 4695
ApoI r'AATT_y
Cuts at: 1493 2458 2738 2860 3143 3160 3386 3538 5647
Size: 965 280 122 283 17 226 152 2109
Cuts at: 5647 1493
Size: 1697
Fragments arranged by size:
2109 1697 965 283 280 226 152 122
17
AseI AT'TA_AT
Cuts at: 160 1032 4405 160
Size: 872 3373 1606
Fragments arranged by size:
3373 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5851
AvaI C'yCGr_G
Cuts at: 1870 1943 2418 3176 1870
Size: 73 475 758 4545
Fragments arranged by size:
4545 758 475 73
BamHI G'GATC_C
Cuts at: 3181 3548 3181
Size: 367 5484
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3172 4627 618
Size: 325 217 627 1385 1455 1842
Fragments arranged by size:
1842 1455 1385 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3170 1391
Size: 1779 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4880
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4042 4076 1903
Size: 34 2105 34 3678
Fragments arranged by size:
3678 2105 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3743 4953 3743
Size: 1210 4641
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5851
BfrBI ATG'CAT
Cuts at: 2192 2192
Size: 5851
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4462 136
Size: 107 122 71 631 605 2790 1525
Fragments arranged by size:
2790 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5846 5846
Size: 5851
BlpI GC'TnA_GC
Cuts at: 3268 3268
Size: 5851
Bme1580I G_kGCm'C
Cuts at: 1790 3912 5068 1790
Size: 2122 1156 2573
Fragments arranged by size:
2573 2122 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5851
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3582 4540 442
Size: 416 53 83 399 24 2165 958 1753
Fragments arranged by size:
2165 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5851
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4511 1239
Size: 3272 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3976 4841 5139 3976
Size: 865 298 4688
Fragments arranged by size:
4688 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4514 882
Size: 617 3015 2219
Fragments arranged by size:
3015 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2893 3642 493
Size: 711 1689 749 2702
Fragments arranged by size:
2702 1689 749 711
BsaBI GATnn'nnATC
Cuts at: 5845 5845
Size: 5851
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3658 4040 4675 275
Size: 53 83 186 936 2125 382 635 1451
Fragments arranged by size:
2125 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4284 4682 4956 5103 5514 4284
Size: 398 274 147 411 4621
Fragments arranged by size:
4621 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5626
Fragments arranged by size:
5626 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2786 1186
Size: 1600 4251
BseYI C'CCAG_C
Cuts at: 5054 5550 5054
Size: 496 5355
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3060 1163
Size: 573 1324 3954
Fragments arranged by size:
3954 1324 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3301 4062 5090 1540
Size: 275 186 1300 761 1028 2301
Fragments arranged by size:
2301 1300 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3912 3997 5068 3912
Size: 85 1071 4695
Fragments arranged by size:
4695 1071 85
BsmI GAATG_Cn'
Cuts at: 2479 3362 3455 2479
Size: 883 93 4875
Fragments arranged by size:
4875 883 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3170 3912 3997 5068 1391
Size: 399 1380 742 85 1071 2174
Fragments arranged by size:
2174 1380 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5514 5514
Size: 5851
BspHI T'CATG_A
Cuts at: 3740 5470 3740
Size: 1730 4121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3206 844
Size: 2362 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3738 1394
Size: 429 1915 3507
Fragments arranged by size:
3507 1915 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2650 2964 4346 4520 65
Size: 1183 1402 314 1382 174 1396
Fragments arranged by size:
1402 1396 1382 1183 314 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4495 4682 1700
Size: 110 2685 187 2869
Fragments arranged by size:
2869 2685 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5851
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5851
BssSI C'ACGA_G
Cuts at: 3911 4923 3911
Size: 1012 4839
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2879 794
Size: 2085 3766
BstBI TT'CG_AA
Cuts at: 4655 4655
Size: 5851
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5851
BstYI r'GATC_y
Cuts at: 1972 3181 3548 3933 3950 5402 5488 5500 5846
Size: 1209 367 385 17 1452 86 12 346
Cuts at: 5846 1972
Size: 1977
Fragments arranged by size:
1977 1452 1209 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5299
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5112
Fragments arranged by size:
5112 683 56
BtsI GCAGTG_nn'
Cuts at: 3043 3367 4161 4181 3043
Size: 324 794 20 4713
Fragments arranged by size:
4713 794 324 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5851
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5816
DraI TTT'AAA
Cuts at: 3155 3155
Size: 5851
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4858 809
Size: 507 3542 1802
Fragments arranged by size:
3542 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2331 3298 4187
Size: 54 1425 50 275 18 501 967 889
Cuts at: 4187 5816 8
Size: 1629 43
Fragments arranged by size:
1629 1425 967 889 501 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3298 1537
Size: 275 1486 4090
Fragments arranged by size:
4090 1486 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2763 3781 1353
Size: 1410 1018 3423
Fragments arranged by size:
3423 1410 1018
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4438 4812 4958 387
Size: 1064 138 2849 374 146 1280
Fragments arranged by size:
2849 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2438 2698 2951 3914 4511 5298 5566
Size: 393 806 260 253 963 597 787 268
Cuts at: 5566 1239
Size: 1524
Fragments arranged by size:
1524 963 806 787 597 393 268 260
253
EcoNI CCTnn'n_nnAGG
Cuts at: 2692 2692
Size: 5851
EcoRI G'AATT_C
Cuts at: 3143 3160 3538 3143
Size: 17 378 5456
Fragments arranged by size:
5456 378 17
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2468 2542 2468
Size: 74 5777
FspI TGC'GCA
Cuts at: 4357 4357
Size: 5851
HaeII r_GCGC'y
Cuts at: 1678 4998 1678
Size: 3320 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2399 2431 4492 4524 4566
Size: 32 135 32 827 32 2061 32 42
Cuts at: 4566 4598 1373
Size: 32 2626
Fragments arranged by size:
2626 2061 827 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3092 3195 3441 669
Size: 988 281 1154 103 246 3079
Fragments arranged by size:
3079 1154 988 281 246 103
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5851
HpaI GTT'AAC
Cuts at: 3441 3441
Size: 5851
KpnI G_GTAC'C
Cuts at: 3176 3176
Size: 5851
MfeI C'AATT_G
Cuts at: 3450 3450
Size: 5851
MluI A'CGCG_T
Cuts at: 5727 5727
Size: 5851
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2127 4965 5149 1305
Size: 822 2838 184 2007
Fragments arranged by size:
2838 2007 822 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2333 10
Size: 54 1425 343 501 3528
Fragments arranged by size:
3528 1425 501 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3809 4168 4327 518
Size: 648 2643 359 159 2042
Fragments arranged by size:
2643 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3267 3944 5092 5337 1606
Size: 1661 677 1148 245 2120
Fragments arranged by size:
2120 1661 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5299
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5851
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5851
NotI GC'GGCC_GC
Cuts at: 3298 3298
Size: 5851
NsiI A_TGCA'T
Cuts at: 2194 2194
Size: 5851
NspI r_CATG'y
Cuts at: 1132 1229 2075 3209 4754 1132
Size: 97 846 1134 1545 2229
Fragments arranged by size:
2229 1545 1134 846 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5851
PciI A'CATG_T
Cuts at: 4750 4750
Size: 5851
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5851
PmeI GTTT'AAAC
Cuts at: 3155 3155
Size: 5851
PmlI CAC'GTG
Cuts at: 2893 2893
Size: 5851
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3920 3952 5433 5465 3920
Size: 32 1481 32 4306
Fragments arranged by size:
4306 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5851
PsiI TTA'TAA
Cuts at: 3421 3421
Size: 5851
PstI C_TGCA'G
Cuts at: 830 3203 830
Size: 2373 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5851
SalI G'TCGA_C
Cuts at: 1936 3193 1936
Size: 1257 4594
SbfI CC_TGCA'GG
Cuts at: 3203 3203
Size: 5851
ScaI AGT'ACT
Cuts at: 4099 5811 4099
Size: 1712 4139
SfcI C'TryA_G
Cuts at: 826 1045 3199 4334 5015 5206 826
Size: 219 2154 1135 681 191 1471
Fragments arranged by size:
2154 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5851
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5851
SmaI CCC'GGG
Cuts at: 1872 3178 1872
Size: 1306 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2418 3955 4856 5118 820
Size: 197 926 475 1537 901 262 1553
Fragments arranged by size:
1553 1537 926 901 475 262 197
SnaBI TAC'GTA
Cuts at: 493 3642 493
Size: 3149 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5851
SphI G_CATG'C
Cuts at: 1132 2075 3209 1132
Size: 943 1134 3774
Fragments arranged by size:
3774 1134 943
SspI AAT'ATT
Cuts at: 5 52 2122 2365 3775 5
Size: 47 2070 243 1410 2081
Fragments arranged by size:
2081 2070 1410 243 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5851
StyI C'CwwG_G
Cuts at: 513 1065 2382 2633 3290 513
Size: 552 1317 251 657 3074
Fragments arranged by size:
3074 1317 657 552 251
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3878 3895 4048 4233 4678 325
Size: 1255 2298 17 153 185 445 1498
Fragments arranged by size:
2298 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3114 4097 5809
Size: 274 80 33 51 1443 1137 983 1712
Cuts at: 5809 96
Size: 138
Fragments arranged by size:
1712 1443 1137 983 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5811 5843 5811
Size: 32 5819
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5851
XbaI T'CTAG_A
Cuts at: 3028 3187 3028
Size: 159 5692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2844 2844
Size: 5851
XhoI C'TCGA_G
Cuts at: 1943 2418 1943
Size: 475 5376
XmaI C'CCGG_G
Cuts at: 1870 3176 1870
Size: 1306 4545
XmnI GAAnn'nnTTC
Cuts at: 3980 3980
Size: 5851
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3659 276
Size: 53 83 186 936 2125 2468
Fragments arranged by size:
2468 2125 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI