Restriction map for
pFN21AB7273
(Circular) (Six-base) MAPSORT of: pFN21AB7273.seq Check: 1360 from: 1 to: 8701
pFN21AB7273 8701 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3193 6511 278
Size: 53 83 186 936 1657 3318 2468
Fragments arranged by size:
3318 2468 1657 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3193 4647 6044 1937
Size: 1256 1454 1397 4594
Fragments arranged by size:
4594 1454 1397 1256
Acc65I G'GTAC_C
Cuts at: 5812 6022 5812
Size: 210 8491
AclI AA'CG_TT
Cuts at: 1563 6828 7201 1563
Size: 5265 373 3063
Fragments arranged by size:
5265 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2020 3612 3735 4832 5280 5690 6764 8148
Size: 388 1592 123 1097 448 410 1074 1384
Cuts at: 8148 8416 1632
Size: 268 1917
Fragments arranged by size:
1917 1592 1384 1097 1074 448 410 388
268 123
AflII C'TTAA_G
Cuts at: 820 1017 4754 820
Size: 197 3737 4767
Fragments arranged by size:
4767 3737 197
AflIII A'CryG_T
Cuts at: 1203 2526 5765 7600 8577 1203
Size: 1323 3239 1835 977 1327
Fragments arranged by size:
3239 1835 1327 1323 977
AgeI A'CCGG_T
Cuts at: 7532 7532
Size: 8701
AhdI GACnn_n'nnGTC
Cuts at: 2072 7430 2072
Size: 5358 3343
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8701
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3549 1184
Size: 2365 6336
AlwNI CAG_nnn'CTG
Cuts at: 5288 5337 6444 8016 5288
Size: 49 1107 1572 5973
Fragments arranged by size:
5973 1572 1107 49
ApaLI G'TGCA_C
Cuts at: 6758 7914 6758
Size: 1156 7545
ApoI r'AATT_y
Cuts at: 1493 3165 3270 3326 3476 3498 3945 4447 4456
Size: 1672 105 56 150 22 447 502 9
Cuts at: 4456 4528 4569 4633 4695 4908 5485 5773 5893
Size: 72 41 64 62 213 577 288 120
Cuts at: 5893 6010 6236 6388 8497 1493
Size: 117 226 152 2109 1697
Fragments arranged by size:
2109 1697 1672 577 502 447 288 226
213 152 150 120 117 105 72 64
62 56 41 22 9
AseI AT'TA_AT
Cuts at: 160 1032 3824 7255 160
Size: 872 2792 3431 1606
Fragments arranged by size:
3431 2792 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8701
AvaI C'yCGr_G
Cuts at: 1870 1943 6026 1870
Size: 73 4083 4545
Fragments arranged by size:
4545 4083 73
AvrII C'CTAG_G
Cuts at: 5081 5081
Size: 8701
BamHI G'GATC_C
Cuts at: 6031 6398 6031
Size: 367 8334
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2014 3031 3359 5812 6022
Size: 325 217 627 227 1017 328 2453 210
Cuts at: 6022 7477 618
Size: 1455 1842
Fragments arranged by size:
2453 1842 1455 1017 627 328 325 227
217 210
BanII G_rGCy'C
Cuts at: 1391 5433 6020 1391
Size: 4042 587 4072
Fragments arranged by size:
4072 4042 587
BbeI G_GCGC'C
Cuts at: 2018 2018
Size: 8701
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2237 2796 3108 3771 4820 928
Size: 971 338 559 312 663 1049 4809
Fragments arranged by size:
4809 1049 971 663 559 338 312
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6892 6926 1903
Size: 34 50 34 4871 34 3678
Fragments arranged by size:
4871 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2392 3559 6593 7803 2392
Size: 1167 3034 1210 3290
Fragments arranged by size:
3290 3034 1210 1167
BclI T'GATC_A
Cuts at: 1548 2533 2661 3402 5470 1548
Size: 985 128 741 2068 4779
Fragments arranged by size:
4779 2068 985 741 128
BfrBI ATG'CAT
Cuts at: 2184 3536 4512 2184
Size: 1352 976 6373
Fragments arranged by size:
6373 1352 976
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5139 7312 136
Size: 107 122 71 631 605 3467 2173 1525
Fragments arranged by size:
3467 2173 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8696 8696
Size: 8701
BlpI GC'TnA_GC
Cuts at: 3920 6118 3920
Size: 2198 6503
Bme1580I G_kGCm'C
Cuts at: 1790 6762 7918 1790
Size: 4972 1156 2573
Fragments arranged by size:
4972 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3020 4355 1318
Size: 1702 1335 5664
Fragments arranged by size:
5664 1702 1335
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5729 6432 7390
Size: 416 53 83 399 24 4312 703 958
Cuts at: 7390 442
Size: 1753
Fragments arranged by size:
4312 1753 958 703 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8701
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2431 5559 2431
Size: 3128 5573
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3219 4923 5346 5658 5850 7361 1239
Size: 1980 1704 423 312 192 1511 2579
Fragments arranged by size:
2579 1980 1704 1511 423 312 192
Bpu10I CC'TnA_GC
Cuts at: 2830 4227 2830
Size: 1397 7304
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6826 7691 7989 6826
Size: 865 298 7538
Fragments arranged by size:
7538 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2080 7364 882
Size: 617 581 5284 2219
Fragments arranged by size:
5284 2219 617 581
BsaAI yAC'GTr
Cuts at: 493 1204 5766 6492 493
Size: 711 4562 726 2702
Fragments arranged by size:
4562 2702 726 711
BsaBI GATnn'nnATC
Cuts at: 5322 5514 8695 5322
Size: 192 3181 5328
Fragments arranged by size:
5328 3181 192
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2015 3190 6508 6890
Size: 53 83 186 936 482 1175 3318 382
Cuts at: 6890 7525 275
Size: 635 1451
Fragments arranged by size:
3318 1451 1175 936 635 482 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 7134 7532 7806 7953 8364 7134
Size: 398 274 147 411 7471
Fragments arranged by size:
7471 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3192 3222 3798 3828 5788
Size: 30 165 30 1789 30 576 30 1960
Cuts at: 5788 5818 1178
Size: 30 4061
Fragments arranged by size:
4061 1960 1789 576 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 2231 4894 4975 5433 5436 5445 5535
Size: 871 174 2663 81 458 3 9 90
Cuts at: 5535 5538 5778 1186
Size: 3 240 4109
Fragments arranged by size:
4109 2663 871 458 240 174 90 81
9 3 3
BseYI C'CCAG_C
Cuts at: 2190 7904 8400 2190
Size: 5714 496 2491
Fragments arranged by size:
5714 2491 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3474 4032 4647 1163
Size: 573 1738 558 615 5217
Fragments arranged by size:
5217 1738 615 573 558
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6151 6912 7940 1540
Size: 275 186 4150 761 1028 2301
Fragments arranged by size:
4150 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3528 5835 6762 6847 7918 3528
Size: 2307 927 85 1071 4311
Fragments arranged by size:
4311 2307 1071 927 85
BsmI GAATG_Cn'
Cuts at: 6212 6305 6212
Size: 93 8608
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3528 5433 5835 6020 6762 6847 7918
Size: 399 1738 1905 402 185 742 85 1071
Cuts at: 7918 1391
Size: 2174
Fragments arranged by size:
2174 1905 1738 1071 742 402 399 185
85
BspEI T'CCGG_A
Cuts at: 8364 8364
Size: 8701
BspHI T'CATG_A
Cuts at: 2920 3138 3483 4326 5473 6590 8320 2920
Size: 218 345 843 1147 1117 1730 3301
Fragments arranged by size:
3301 1730 1147 1117 843 345 218
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2959 6056 844
Size: 2115 3097 3489
Fragments arranged by size:
3489 3097 2115
BsrBI CCG'CTC
Cuts at: 1394 1823 6588 1394
Size: 429 4765 3507
Fragments arranged by size:
4765 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2262 2976 7196 7370 65
Size: 1183 1014 714 4220 174 1396
Fragments arranged by size:
4220 1396 1183 1014 714 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7345 7532 1700
Size: 110 5535 187 2869
Fragments arranged by size:
5535 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 5961 96
Size: 5865 2836
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8701
BssSI C'ACGA_G
Cuts at: 6761 7773 6761
Size: 1012 7689
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2279 794
Size: 1485 7216
BstBI TT'CG_AA
Cuts at: 7505 7505
Size: 8701
BstEII G'GTnAC_C
Cuts at: 1184 5165 1184
Size: 3981 4720
BstXI CCAn_nnnn'nTGG
Cuts at: 4284 4284
Size: 8701
BstYI r'GATC_y
Cuts at: 1972 3202 3856 4062 5323 5632 6031 6398 6783
Size: 1230 654 206 1261 309 399 367 385
Cuts at: 6783 6800 8252 8338 8350 8696 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1261 1230 654 399 385 367
346 309 206 86 17 12
BstZ17I GTA'TAC
Cuts at: 4648 4648
Size: 8701
Bsu36I CC'TnA_GG
Cuts at: 5149 5149
Size: 8701
BtgI C'CryG_G
Cuts at: 513 1065 4986 513
Size: 552 3921 4228
Fragments arranged by size:
4228 3921 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7962
Fragments arranged by size:
7962 683 56
BtsI GCAGTG_nn'
Cuts at: 4673 6217 7011 7031 4673
Size: 1544 794 20 6343
Fragments arranged by size:
6343 1544 794 20
ClaI AT'CG_AT
Cuts at: 1554 3285 1554
Size: 1731 6970
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3897 3932 529
Size: 35 3333 35 5298
Fragments arranged by size:
5298 3333 35 35
DraI TTT'AAA
Cuts at: 2430 2992 4735 4842 6005 2430
Size: 562 1743 107 1163 5126
Fragments arranged by size:
5126 1743 1163 562 107
DraIII CAC_nnn'GTG
Cuts at: 4775 4775
Size: 8701
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7708 809
Size: 507 6392 1802
Fragments arranged by size:
6392 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2943 3424 6148
Size: 54 1425 50 275 18 1113 481 2724
Cuts at: 6148 7037 8666 8
Size: 889 1629 43
Fragments arranged by size:
2724 1629 1425 1113 889 481 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6148 1537
Size: 275 4336 4090
Fragments arranged by size:
4336 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2124 2145 2856 3633 5421 5454 5643 6631
Size: 771 21 711 777 1788 33 189 988
Cuts at: 6631 1353
Size: 3423
Fragments arranged by size:
3423 1788 988 777 771 711 189 33
21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5607 7288 7662 7808 387
Size: 1064 138 4018 1681 374 146 1280
Fragments arranged by size:
4018 1681 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2020 3219 3612 3735 4832 4923 5280
Size: 393 388 1199 393 123 1097 91 357
Cuts at: 5280 5346 5658 5690 5850 6764 7361 8148 8416
Size: 66 312 32 160 914 597 787 268
Cuts at: 8416 1239
Size: 1524
Fragments arranged by size:
1524 1199 1097 914 787 597 393 393
388 357 312 268 160 123 91 66
32
EcoNI CCTnn'n_nnAGG
Cuts at: 2087 5127 5806 2087
Size: 3040 679 4982
Fragments arranged by size:
4982 3040 679
EcoRI G'AATT_C
Cuts at: 3326 5893 6010 6388 3326
Size: 2567 117 378 5639
Fragments arranged by size:
5639 2567 378 117
EcoRV GAT'ATC
Cuts at: 2722 2722
Size: 8701
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2705 5407 2705
Size: 2702 5999
FspI TGC'GCA
Cuts at: 7207 7207
Size: 8701
HaeII r_GCGC'y
Cuts at: 1678 2018 7848 1678
Size: 340 5830 2531
Fragments arranged by size:
5830 2531 340
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2058 2090 2224 2256 2261
Size: 32 135 32 486 32 134 32 5
Cuts at: 2261 2293 4321 4353 5219 5251 5428 5460 7342
Size: 32 2028 32 866 32 177 32 1882
Cuts at: 7342 7374 7416 7448 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2028 1882 866 486 177 135 134
42 32 32 32 32 32 32 32
32 32 32 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3194 3837 6045 6291 669
Size: 988 281 1256 643 2208 246 3079
Fragments arranged by size:
3079 2208 1256 988 643 281 246
HindIII A'AGCT_T
Cuts at: 748 5203 748
Size: 4455 4246
HpaI GTT'AAC
Cuts at: 3837 6291 3837
Size: 2454 6247
KasI G'GCGC_C
Cuts at: 2014 2014
Size: 8701
KpnI G_GTAC'C
Cuts at: 5816 6026 5816
Size: 210 8491
MfeI C'AATT_G
Cuts at: 2247 3074 4194 4278 6300 2247
Size: 827 1120 84 2022 4648
Fragments arranged by size:
4648 2022 1120 827 84
MluI A'CGCG_T
Cuts at: 8577 8577
Size: 8701
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4069 5192 7815 7999 1305
Size: 2764 1123 2623 184 2007
Fragments arranged by size:
2764 2623 2007 1123 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2945 3426 10
Size: 54 1425 343 1113 481 5285
Fragments arranged by size:
5285 1425 1113 481 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2492 2669 3531 3849 4101 6659 7018
Size: 648 1326 177 862 318 252 2558 359
Cuts at: 7018 7177 518
Size: 159 2042
Fragments arranged by size:
2558 2042 1326 862 648 359 318 252
177 159
MspA1I CmG'CkG
Cuts at: 1606 2167 2420 2841 4360 6117 6794 7942 8187
Size: 561 253 421 1519 1757 677 1148 245
Cuts at: 8187 1606
Size: 2120
Fragments arranged by size:
2120 1757 1519 1148 677 561 421 253
245
NarI GG'CG_CC
Cuts at: 2015 2015
Size: 8701
NcoI C'CATG_G
Cuts at: 513 1065 4986 513
Size: 552 3921 4228
Fragments arranged by size:
4228 3921 552
NdeI CA'TA_TG
Cuts at: 387 3846 387
Size: 3459 5242
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8701
NotI GC'GGCC_GC
Cuts at: 6148 6148
Size: 8701
NsiI A_TGCA'T
Cuts at: 2186 3538 4514 2186
Size: 1352 976 6373
Fragments arranged by size:
6373 1352 976
NspI r_CATG'y
Cuts at: 1132 1229 2530 3087 3536 6059 7604 1132
Size: 97 1301 557 449 2523 1545 2229
Fragments arranged by size:
2523 2229 1545 1301 557 449 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8701
PciI A'CATG_T
Cuts at: 2526 7600 2526
Size: 5074 3627
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8701
PfoI T'CCnGG_A
Cuts at: 5110 5110
Size: 8701
PmeI GTTT'AAAC
Cuts at: 6005 6005
Size: 8701
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5559 5591 6770 6802 8283 8315 5559
Size: 32 1179 32 1481 32 5945
Fragments arranged by size:
5945 1481 1179 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8701
PsiI TTA'TAA
Cuts at: 5687 6271 5687
Size: 584 8117
PstI C_TGCA'G
Cuts at: 830 2956 4680 6053 830
Size: 2126 1724 1373 3478
Fragments arranged by size:
3478 2126 1724 1373
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8701
PvuII CAG'CTG
Cuts at: 2167 2420 2841 4360 2167
Size: 253 421 1519 6508
Fragments arranged by size:
6508 1519 421 253
SalI G'TCGA_C
Cuts at: 1936 3192 6043 1936
Size: 1256 2851 4594
Fragments arranged by size:
4594 2851 1256
SapI GCTCTTCn'nnn_
Cuts at: 2145 2856 5421 2145
Size: 711 2565 5425
Fragments arranged by size:
5425 2565 711
SbfI CC_TGCA'GG
Cuts at: 6053 6053
Size: 8701
ScaI AGT'ACT
Cuts at: 3643 4551 6949 8661 3643
Size: 908 2398 1712 3683
Fragments arranged by size:
3683 2398 1712 908
SfcI C'TryA_G
Cuts at: 826 1045 2283 2952 3813 4676 6049 7184 7865
Size: 219 1238 669 861 863 1373 1135 681
Cuts at: 7865 8056 826
Size: 191 1471
Fragments arranged by size:
1471 1373 1238 1135 863 861 681 669
219 191
SfoI GGC'GCC
Cuts at: 2016 2016
Size: 8701
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8701
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8701
SmaI CCC'GGG
Cuts at: 1872 6028 1872
Size: 4156 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4754 6805 7706 7968 820
Size: 197 926 2811 2051 901 262 1553
Fragments arranged by size:
2811 2051 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6492 493
Size: 5999 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8701
SphI G_CATG'C
Cuts at: 1132 3536 6059 1132
Size: 2404 2523 3774
Fragments arranged by size:
3774 2523 2404
SspI AAT'ATT
Cuts at: 5 52 3573 4252 4807 6625 5
Size: 47 3521 679 555 1818 2081
Fragments arranged by size:
3521 2081 1818 679 555 47
StuI AGG'CCT
Cuts at: 1517 5122 1517
Size: 3605 5096
StyI C'CwwG_G
Cuts at: 513 1065 2195 3148 4986 5081 5490 5807 5885
Size: 552 1130 953 1838 95 409 317 78
Cuts at: 5885 6140 513
Size: 255 3074
Fragments arranged by size:
3074 1838 1130 953 552 409 317 255
95 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2259 3177 4832 5184 6728 6745 6898
Size: 1255 679 918 1655 352 1544 17 153
Cuts at: 6898 7083 7528 325
Size: 185 445 1498
Fragments arranged by size:
1655 1544 1498 1255 918 679 445 352
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2401 3641 4051
Size: 274 80 33 51 1443 424 1240 410
Cuts at: 4051 4549 5961 6947 8659 96
Size: 498 1412 986 1712 138
Fragments arranged by size:
1712 1443 1412 1240 986 498 424 410
274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2957 2989 3801 3833 5788 5820 8661 8693 2957
Size: 32 812 32 1955 32 2841 32 2965
Fragments arranged by size:
2965 2841 1955 812 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8701
XbaI T'CTAG_A
Cuts at: 3646 6037 3646
Size: 2391 6310
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2253 4281 2253
Size: 2028 6673
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8701
XmaI C'CCGG_G
Cuts at: 1870 6026 1870
Size: 4156 4545
XmnI GAAnn'nnTTC
Cuts at: 2685 4598 6830 2685
Size: 1913 2232 4556
Fragments arranged by size:
4556 2232 1913
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3191 6509 276
Size: 53 83 186 936 1657 3318 2468
Fragments arranged by size:
3318 2468 1657 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbvCI BsiWI
BsmBI EcoICRI EcoO109I FseI FspAI NaeI NgoMIV NruI
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SexAI SfiI SrfI SwaI