Restriction map for
pFN21AB7272
(Circular) (Six-base) MAPSORT of: pFN21AB7272.seq Check: 3323 from: 1 to: 6031
pFN21AB7272 6031 bp
With 182 enzymes: SgfI *
April 15, 2010 09:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2358 2358
Size: 6031
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3841 278
Size: 53 83 186 936 2305 2468
Fragments arranged by size:
2468 2305 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3374 1937
Size: 1437 4594
Acc65I G'GTAC_C
Cuts at: 3352 3352
Size: 6031
AclI AA'CG_TT
Cuts at: 1563 4158 4531 1563
Size: 2595 373 3063
Fragments arranged by size:
3063 2595 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2294 2663 2796 4094 5478 5746 1632
Size: 662 369 133 1298 1384 268 1917
Fragments arranged by size:
1917 1384 1298 662 369 268 133
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5834
AflIII A'CryG_T
Cuts at: 1203 4930 5907 1203
Size: 3727 977 1327
Fragments arranged by size:
3727 1327 977
AgeI A'CCGG_T
Cuts at: 4862 4862
Size: 6031
AhdI GACnn_n'nnGTC
Cuts at: 4760 4760
Size: 6031
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6031
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2366 1184
Size: 1182 4849
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2618 2650 2618
Size: 32 5999
AlwNI CAG_nnn'CTG
Cuts at: 2137 2217 2570 2980 3774 5346 2137
Size: 80 353 410 794 1572 2822
Fragments arranged by size:
2822 1572 794 410 353 80
ApaI G_GGCC'C
Cuts at: 2102 2550 2102
Size: 448 5583
ApaLI G'TGCA_C
Cuts at: 3257 4088 5244 3257
Size: 831 1156 4044
Fragments arranged by size:
4044 1156 831
ApoI r'AATT_y
Cuts at: 1493 2407 2970 3340 3566 3718 5827 1493
Size: 914 563 370 226 152 2109 1697
Fragments arranged by size:
2109 1697 914 563 370 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4585 160
Size: 872 3553 1606
Fragments arranged by size:
3553 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6031
AvaI C'yCGr_G
Cuts at: 1870 1943 3264 3356 1870
Size: 73 1321 92 4545
Fragments arranged by size:
4545 1321 92 73
BamHI G'GATC_C
Cuts at: 3361 3728 3361
Size: 367 5664
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2070 3352 4807 618
Size: 325 217 627 283 1282 1455 1842
Fragments arranged by size:
1842 1455 1282 627 325 283 217
BanII G_rGCy'C
Cuts at: 1391 2102 2550 2662 2850 3350 1391
Size: 711 448 112 188 500 4072
Fragments arranged by size:
4072 711 500 448 188 112
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5060
BbvCI CC'TCA_GC
Cuts at: 3251 3251
Size: 6031
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2509 2543 4222 4256 1903
Size: 34 50 34 488 34 1679 34 3678
Fragments arranged by size:
3678 1679 488 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3923 5133 3923
Size: 1210 4821
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6031
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2039 4642
Size: 107 122 71 631 605 337 30 2603
Cuts at: 4642 136
Size: 1525
Fragments arranged by size:
2603 1525 631 605 337 122 107 71
30
BglII A'GATC_T
Cuts at: 6026 6026
Size: 6031
BlpI GC'TnA_GC
Cuts at: 2138 3448 2138
Size: 1310 4721
Bme1580I G_kGCm'C
Cuts at: 1790 2102 2550 3261 4092 5248 1790
Size: 312 448 711 831 1156 2573
Fragments arranged by size:
2573 1156 831 711 448 312
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6031
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2146 2401 3762
Size: 416 53 83 399 24 729 255 1361
Cuts at: 3762 4720 442
Size: 958 1753
Fragments arranged by size:
1753 1361 958 729 416 399 255 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6031
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2958 3100 4691 1239
Size: 1719 142 1591 2579
Fragments arranged by size:
2579 1719 1591 142
Bpu10I CC'TnA_GC
Cuts at: 2571 3251 2571
Size: 680 5351
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4156 5021 5319 4156
Size: 865 298 4868
Fragments arranged by size:
4868 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2948 4694 882
Size: 617 1449 1746 2219
Fragments arranged by size:
2219 1746 1449 617
BsaAI yAC'GTr
Cuts at: 493 1204 3822 493
Size: 711 2618 2702
Fragments arranged by size:
2702 2618 711
BsaBI GATnn'nnATC
Cuts at: 6025 6025
Size: 6031
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3838 4220 4855 275
Size: 53 83 186 936 2305 382 635 1451
Fragments arranged by size:
2305 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4464 4862 5136 5283 5694 4464
Size: 398 274 147 411 4801
Fragments arranged by size:
4801 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2618 2648 1178
Size: 30 165 30 1215 30 4561
Fragments arranged by size:
4561 1215 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3044 3161 1186
Size: 1858 117 4056
Fragments arranged by size:
4056 1858 117
BseYI C'CCAG_C
Cuts at: 3223 5234 5730 3223
Size: 2011 496 3524
Fragments arranged by size:
3524 2011 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2590 1163
Size: 573 854 4604
Fragments arranged by size:
4604 854 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2034 2161 3481 4242 5270 1540
Size: 275 186 33 127 1320 761 1028 2301
Fragments arranged by size:
2301 1320 1028 761 275 186 127 33
BsiHKAI G_wGCw'C
Cuts at: 2144 2662 3261 4092 4177 5248 2144
Size: 518 599 831 85 1071 2927
Fragments arranged by size:
2927 1071 831 599 518 85
BsmI GAATG_Cn'
Cuts at: 3542 3635 3542
Size: 93 5938
BsmBI CGTCTCn'nnnn_
Cuts at: 2152 2181 2152
Size: 29 6002
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2102 2144 2550 2662 2850 3261 3350
Size: 399 312 42 406 112 188 411 89
Cuts at: 3350 4092 4177 5248 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 411 406 399 312 188
112 89 85 42
BspEI T'CCGG_A
Cuts at: 5694 5694
Size: 6031
BspHI T'CATG_A
Cuts at: 3920 5650 3920
Size: 1730 4301
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2358 2709 3386 844
Size: 1514 351 677 3489
Fragments arranged by size:
3489 1514 677 351
BsrBI CCG'CTC
Cuts at: 1394 1823 3918 1394
Size: 429 2095 3507
Fragments arranged by size:
3507 2095 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4526 4700 65
Size: 1183 3278 174 1396
Fragments arranged by size:
3278 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2008 2067 4675 4862 1700
Size: 110 198 59 2608 187 2869
Fragments arranged by size:
2869 2608 198 187 110 59
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6031
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6031
BssSI C'ACGA_G
Cuts at: 2666 2872 4091 5103 2666
Size: 206 1219 1012 3594
Fragments arranged by size:
3594 1219 1012 206
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6031
BstBI TT'CG_AA
Cuts at: 2405 4835 2405
Size: 2430 3601
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6031
BstXI CCAn_nnnn'nTGG
Cuts at: 2833 2936 2833
Size: 103 5928
BstYI r'GATC_y
Cuts at: 1972 3361 3728 4113 4130 5582 5668 5680 6026
Size: 1389 367 385 17 1452 86 12 346
Cuts at: 6026 1972
Size: 1977
Fragments arranged by size:
1977 1452 1389 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3160 3160
Size: 6031
BtgI C'CryG_G
Cuts at: 513 1065 2037 2107 2433 2448 3058 3186 513
Size: 552 972 70 326 15 610 128 3358
Fragments arranged by size:
3358 972 610 552 326 128 70 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5292
Fragments arranged by size:
5292 683 56
BtsI GCAGTG_nn'
Cuts at: 3080 3181 3547 4341 4361 3080
Size: 101 366 794 20 4750
Fragments arranged by size:
4750 794 366 101 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6031
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5996
DraI TTT'AAA
Cuts at: 3335 3335
Size: 6031
DraIII CAC_nnn'GTG
Cuts at: 2299 3054 2299
Size: 755 5276
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5038 809
Size: 507 3722 1802
Fragments arranged by size:
3722 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 2467 3478
Size: 54 1425 50 275 18 201 436 1011
Cuts at: 3478 4367 5996 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1011 889 436 275 201 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2031 3478 1537
Size: 275 219 1447 4090
Fragments arranged by size:
4090 1447 275 219
EarI CTCTTCn'nnn_
Cuts at: 1353 3138 3961 1353
Size: 1785 823 3423
Fragments arranged by size:
3423 1785 823
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4618 4992 5138 387
Size: 1064 138 3029 374 146 1280
Fragments arranged by size:
3029 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2660 2660
Size: 6031
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2294 2663 2796 2958 3100 4094 4691
Size: 393 662 369 133 162 142 994 597
Cuts at: 4691 5478 5746 1239
Size: 787 268 1524
Fragments arranged by size:
1524 994 787 662 597 393 369 268
162 142 133
EcoO109I rG'GnC_Cy
Cuts at: 2099 2263 2547 3006 2099
Size: 164 284 459 5124
Fragments arranged by size:
5124 459 284 164
EcoRI G'AATT_C
Cuts at: 2970 3340 3718 2970
Size: 370 378 5283
Fragments arranged by size:
5283 378 370
FspI TGC'GCA
Cuts at: 4537 4537
Size: 6031
HaeII r_GCGC'y
Cuts at: 1678 5178 1678
Size: 3500 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2456 2488 4672 4704 4746
Size: 32 135 32 884 32 2184 32 42
Cuts at: 4746 4778 1373
Size: 32 2626
Fragments arranged by size:
2626 2184 884 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3375 3621 669
Size: 988 281 1437 246 3079
Fragments arranged by size:
3079 1437 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2897 748
Size: 2149 3882
HpaI GTT'AAC
Cuts at: 3621 3621
Size: 6031
KpnI G_GTAC'C
Cuts at: 3356 3356
Size: 6031
MfeI C'AATT_G
Cuts at: 3630 3630
Size: 6031
MluI A'CGCG_T
Cuts at: 5907 5907
Size: 6031
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2377 2727 2760 5145 5329 1305
Size: 1072 350 33 2385 184 2007
Fragments arranged by size:
2385 2007 1072 350 184 33
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2469 10
Size: 54 1425 343 637 3572
Fragments arranged by size:
3572 1425 637 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2485 2670 3989 4348 4507 518
Size: 648 1319 185 1319 359 159 2042
Fragments arranged by size:
2042 1319 1319 648 359 185 159
MspA1I CmG'CkG
Cuts at: 1606 2039 3447 4124 5272 5517 1606
Size: 433 1408 677 1148 245 2120
Fragments arranged by size:
2120 1408 1148 677 433 245
NaeI GCC'GGC
Cuts at: 2010 2069 2010
Size: 59 5972
NcoI C'CATG_G
Cuts at: 513 1065 2448 513
Size: 552 1383 4096
Fragments arranged by size:
4096 1383 552
NdeI CA'TA_TG
Cuts at: 387 3326 387
Size: 2939 3092
NgoMIV G'CCGG_C
Cuts at: 2008 2067 2008
Size: 59 5972
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6031
NotI GC'GGCC_GC
Cuts at: 2031 3478 2031
Size: 1447 4584
NspI r_CATG'y
Cuts at: 1132 1229 3389 4934 1132
Size: 97 2160 1545 2229
Fragments arranged by size:
2229 2160 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2758 1348
Size: 1410 4621
PciI A'CATG_T
Cuts at: 4930 4930
Size: 6031
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2248 1108
Size: 1140 4891
PfoI T'CCnGG_A
Cuts at: 3116 3116
Size: 6031
PmeI GTTT'AAAC
Cuts at: 3335 3335
Size: 6031
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2618 2650 4100 4132 5613 5645 2618
Size: 32 1450 32 1481 32 3004
Fragments arranged by size:
3004 1481 1450 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2263 2263
Size: 6031
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6031
PsiI TTA'TAA
Cuts at: 3601 3601
Size: 6031
PspOMI G'GGCC_C
Cuts at: 2098 2546 2098
Size: 448 5583
PstI C_TGCA'G
Cuts at: 830 3383 830
Size: 2553 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6031
SacI G_AGCT'C
Cuts at: 2662 2662
Size: 6031
SacII CC_GC'GG
Cuts at: 2040 2040
Size: 6031
SalI G'TCGA_C
Cuts at: 1936 3373 1936
Size: 1437 4594
SbfI CC_TGCA'GG
Cuts at: 3383 3383
Size: 6031
ScaI AGT'ACT
Cuts at: 2171 4279 5991 2171
Size: 2108 1712 2211
Fragments arranged by size:
2211 2108 1712
SfcI C'TryA_G
Cuts at: 826 1045 3379 4514 5195 5386 826
Size: 219 2334 1135 681 191 1471
Fragments arranged by size:
2334 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6031
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6031
SmaI CCC'GGG
Cuts at: 1872 3358 1872
Size: 1486 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4135 5036 5298 820
Size: 197 926 2192 901 262 1553
Fragments arranged by size:
2192 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3822 493
Size: 3329 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6031
SphI G_CATG'C
Cuts at: 1132 3389 1132
Size: 2257 3774
SspI AAT'ATT
Cuts at: 5 52 3955 5
Size: 47 3903 2081
Fragments arranged by size:
3903 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6031
StyI C'CwwG_G
Cuts at: 513 1065 2259 2448 2713 3470 513
Size: 552 1194 189 265 757 3074
Fragments arranged by size:
3074 1194 757 552 265 189
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4058 4075 4228 4413 4858 325
Size: 1255 2478 17 153 185 445 1498
Fragments arranged by size:
2478 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2169 4277 5989
Size: 274 80 33 51 1443 192 2108 1712
Cuts at: 5989 96
Size: 138
Fragments arranged by size:
2108 1712 1443 274 192 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3112 3144 5991 6023 3112
Size: 32 2847 32 3120
Fragments arranged by size:
3120 2847 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6031
XbaI T'CTAG_A
Cuts at: 3367 3367
Size: 6031
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2491 2491
Size: 6031
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6031
XmaI C'CCGG_G
Cuts at: 1870 3356 1870
Size: 1486 4545
XmnI GAAnn'nnTTC
Cuts at: 4160 4160
Size: 6031
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3839 276
Size: 53 83 186 936 2305 2468
Fragments arranged by size:
2468 2305 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SacI SacII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BstZ17I EcoNI EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII
SanDI SapI SexAI SfiI SfoI SrfI SwaI