Restriction map for
pFN21AB7271
(Circular) (Six-base) MAPSORT of: pFN21AB7271.seq Check: 1089 from: 1 to: 6190
pFN21AB7271 6190 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4000 278
Size: 53 83 186 936 2464 2468
Fragments arranged by size:
2468 2464 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2057 3111 3533 1937
Size: 120 1054 422 4594
Fragments arranged by size:
4594 1054 422 120
Acc65I G'GTAC_C
Cuts at: 3511 3511
Size: 6190
AclI AA'CG_TT
Cuts at: 1563 4317 4690 1563
Size: 2754 373 3063
Fragments arranged by size:
3063 2754 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4253 5637 5905 1632
Size: 2621 1384 268 1917
Fragments arranged by size:
2621 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 3185 820
Size: 197 2168 3825
Fragments arranged by size:
3825 2168 197
AflIII A'CryG_T
Cuts at: 1203 5089 6066 1203
Size: 3886 977 1327
Fragments arranged by size:
3886 1327 977
AgeI A'CCGG_T
Cuts at: 5021 5021
Size: 6190
AhdI GACnn_n'nnGTC
Cuts at: 4919 4919
Size: 6190
AleI CACnn'nnGTG
Cuts at: 1166 2204 1166
Size: 1038 5152
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2232 1184
Size: 1048 5142
AlwNI CAG_nnn'CTG
Cuts at: 2575 3485 3933 5505 2575
Size: 910 448 1572 3260
Fragments arranged by size:
3260 1572 910 448
ApaLI G'TGCA_C
Cuts at: 4247 5403 4247
Size: 1156 5034
ApoI r'AATT_y
Cuts at: 1493 3499 3725 3877 5986 1493
Size: 2006 226 152 2109 1697
Fragments arranged by size:
2109 2006 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4744 160
Size: 872 3712 1606
Fragments arranged by size:
3712 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6190
AvaI C'yCGr_G
Cuts at: 1870 1943 3454 3515 1870
Size: 73 1511 61 4545
Fragments arranged by size:
4545 1511 73 61
BamHI G'GATC_C
Cuts at: 3520 3887 3520
Size: 367 5823
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3375 3511 4966 618
Size: 325 217 627 1588 136 1455 1842
Fragments arranged by size:
1842 1588 1455 627 325 217 136
BanII G_rGCy'C
Cuts at: 1391 2120 2288 2398 2501 2995 3455 3464 3509
Size: 729 168 110 103 494 460 9 45
Cuts at: 3509 1391
Size: 4072
Fragments arranged by size:
4072 729 494 460 168 110 103 45
9
BbeI G_GCGC'C
Cuts at: 3379 3379
Size: 6190
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3167 928
Size: 971 1268 3951
Fragments arranged by size:
3951 1268 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4381 4415 1903
Size: 34 2444 34 3678
Fragments arranged by size:
3678 2444 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4082 5292 4082
Size: 1210 4980
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6190
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2387 4801 136
Size: 107 122 71 631 605 715 2414 1525
Fragments arranged by size:
2414 1525 715 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6185 6185
Size: 6190
BlpI GC'TnA_GC
Cuts at: 3607 3607
Size: 6190
Bme1580I G_kGCm'C
Cuts at: 1790 2445 3323 4251 5407 1790
Size: 655 878 928 1156 2573
Fragments arranged by size:
2573 1156 928 878 655
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6190
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2499 2869 3921
Size: 416 53 83 399 24 1082 370 1052
Cuts at: 3921 4879 442
Size: 958 1753
Fragments arranged by size:
1753 1082 1052 958 416 399 370 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2048 2354 2514 2904 3054 4850 1239
Size: 809 306 160 390 150 1796 2579
Fragments arranged by size:
2579 1796 809 390 306 160 150
Bpu10I CC'TnA_GC
Cuts at: 2910 2910
Size: 6190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4315 5180 5478 4315
Size: 865 298 5027
Fragments arranged by size:
5027 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2890 4853 882
Size: 617 1391 1963 2219
Fragments arranged by size:
2219 1963 1391 617
BsaAI yAC'GTr
Cuts at: 493 1204 3981 493
Size: 711 2777 2702
Fragments arranged by size:
2777 2702 711
BsaBI GATnn'nnATC
Cuts at: 6184 6184
Size: 6190
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3376 3997 4379 5014
Size: 53 83 186 936 1843 621 382 635
Cuts at: 5014 275
Size: 1451
Fragments arranged by size:
1843 1451 936 635 621 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4623 5021 5295 5442 5853 4623
Size: 398 274 147 411 4960
Fragments arranged by size:
4960 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3296 3326 1178
Size: 30 165 30 1893 30 4042
Fragments arranged by size:
4042 1893 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2265 2268 1186
Size: 1079 3 5108
Fragments arranged by size:
5108 1079 3
BseYI C'CCAG_C
Cuts at: 2425 2444 3234 5393 5889 2425
Size: 19 790 2159 496 2726
Fragments arranged by size:
2726 2159 790 496 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2736 3036 1163
Size: 573 1000 300 4317
Fragments arranged by size:
4317 1000 573 300
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3640 4401 5429 1540
Size: 275 186 1639 761 1028 2301
Fragments arranged by size:
2301 1639 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2120 2398 4251 4336 5407 2120
Size: 278 1853 85 1071 2903
Fragments arranged by size:
2903 1853 1071 278 85
BsmI GAATG_Cn'
Cuts at: 3701 3794 3701
Size: 93 6097
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2120 2288 2398 2445 2501 2995 3323
Size: 399 330 168 110 47 56 494 328
Cuts at: 3323 3455 3464 3509 4251 4336 5407 1391
Size: 132 9 45 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 494 399 330 328 168
132 110 85 56 47 45 9
BspEI T'CCGG_A
Cuts at: 5853 5853
Size: 6190
BspHI T'CATG_A
Cuts at: 4079 5809 4079
Size: 1730 4460
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2763 2956 3545 844
Size: 1919 193 589 3489
Fragments arranged by size:
3489 1919 589 193
BsrBI CCG'CTC
Cuts at: 1394 1823 2403 4077 1394
Size: 429 580 1674 3507
Fragments arranged by size:
3507 1674 580 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4685 4859 65
Size: 1183 3437 174 1396
Fragments arranged by size:
3437 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2538 4834 5021 1700
Size: 110 728 2296 187 2869
Fragments arranged by size:
2869 2296 728 187 110
BsrGI T'GTAC_A
Cuts at: 96 2351 96
Size: 2255 3935
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6190
BssSI C'ACGA_G
Cuts at: 2294 4250 5262 2294
Size: 1956 1012 3222
Fragments arranged by size:
3222 1956 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6190
BstBI TT'CG_AA
Cuts at: 4994 4994
Size: 6190
BstEII G'GTnAC_C
Cuts at: 1184 2144 1184
Size: 960 5230
BstXI CCAn_nnnn'nTGG
Cuts at: 3028 3028
Size: 6190
BstYI r'GATC_y
Cuts at: 1972 2132 3396 3520 3887 4272 4289 5741 5827
Size: 160 1264 124 367 385 17 1452 86
Cuts at: 5827 5839 6185 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1264 385 367 346 160 124
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2958 3105 2958
Size: 147 6043
BtgI C'CryG_G
Cuts at: 513 1065 2004 2358 513
Size: 552 939 354 4345
Fragments arranged by size:
4345 939 552 354
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5451
Fragments arranged by size:
5451 683 56
BtsI GCAGTG_nn'
Cuts at: 3355 3706 4500 4520 3355
Size: 351 794 20 5025
Fragments arranged by size:
5025 794 351 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6190
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2650 2685 529
Size: 35 2086 35 4034
Fragments arranged by size:
4034 2086 35 35
DraI TTT'AAA
Cuts at: 3494 3494
Size: 6190
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5197 809
Size: 507 3881 1802
Fragments arranged by size:
3881 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2388 3637 4526
Size: 54 1425 50 275 18 558 1249 889
Cuts at: 4526 6155 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1249 889 558 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3637 1537
Size: 275 1825 4090
Fragments arranged by size:
4090 1825 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2559 4120 1353
Size: 1206 1561 3423
Fragments arranged by size:
3423 1561 1206
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2991 4777 5151 5297 387
Size: 1064 138 1402 1786 374 146 1280
Fragments arranged by size:
1786 1402 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2118 2396 2118
Size: 278 5912
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 2354 2514 2904 3054 4253 4850
Size: 393 416 306 160 390 150 1199 597
Cuts at: 4850 5637 5905 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1199 787 597 416 393 390 306
268 160 150
EcoNI CCTnn'n_nnAGG
Cuts at: 2909 2984 2909
Size: 75 6115
EcoO109I rG'GnC_Cy
Cuts at: 2945 2945
Size: 6190
EcoRI G'AATT_C
Cuts at: 3499 3877 3499
Size: 378 5812
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2076 2588 3184 2076
Size: 512 596 5082
Fragments arranged by size:
5082 596 512
FspI TGC'GCA
Cuts at: 3347 4696 3347
Size: 1349 4841
HaeII r_GCGC'y
Cuts at: 1678 2927 3379 5337 1678
Size: 1249 452 1958 2531
Fragments arranged by size:
2531 1958 1249 452
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2275 2307 3260 3292 4831
Size: 32 135 32 703 32 953 32 1539
Cuts at: 4831 4863 4905 4937 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1539 953 703 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3534 3780 669
Size: 988 281 1596 246 3079
Fragments arranged by size:
3079 1596 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6190
HpaI GTT'AAC
Cuts at: 3780 3780
Size: 6190
KasI G'GCGC_C
Cuts at: 3375 3375
Size: 6190
KpnI G_GTAC'C
Cuts at: 3515 3515
Size: 6190
MfeI C'AATT_G
Cuts at: 3789 3789
Size: 6190
MluI A'CGCG_T
Cuts at: 6066 6066
Size: 6190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2506 5304 5488 1305
Size: 1201 2798 184 2007
Fragments arranged by size:
2798 2007 1201 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2390 10
Size: 54 1425 343 558 3810
Fragments arranged by size:
3810 1425 558 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2204 2214 4148 4507 4666 518
Size: 648 1038 10 1934 359 159 2042
Fragments arranged by size:
2042 1934 1038 648 359 159 10
MspA1I CmG'CkG
Cuts at: 1606 3234 3606 4283 5431 5676 1606
Size: 1628 372 677 1148 245 2120
Fragments arranged by size:
2120 1628 1148 677 372 245
NaeI GCC'GGC
Cuts at: 2540 2540
Size: 6190
NarI GG'CG_CC
Cuts at: 3376 3376
Size: 6190
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4699
Fragments arranged by size:
4699 939 552
NdeI CA'TA_TG
Cuts at: 387 2012 2211 387
Size: 1625 199 4366
Fragments arranged by size:
4366 1625 199
NgoMIV G'CCGG_C
Cuts at: 2538 2538
Size: 6190
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6190
NotI GC'GGCC_GC
Cuts at: 3637 3637
Size: 6190
NspI r_CATG'y
Cuts at: 1132 1229 2352 2848 3371 3478 3548 5093 1132
Size: 97 1123 496 523 107 70 1545 2229
Fragments arranged by size:
2229 1545 1123 523 496 107 97 70
PasI CC'CwG_GG
Cuts at: 1348 2986 1348
Size: 1638 4552
PciI A'CATG_T
Cuts at: 5089 5089
Size: 6190
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6190
PfoI T'CCnGG_A
Cuts at: 2025 2025
Size: 6190
PmeI GTTT'AAAC
Cuts at: 3494 3494
Size: 6190
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4259 4291 5772 5804 4259
Size: 32 1481 32 4645
Fragments arranged by size:
4645 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2945 2945
Size: 6190
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6190
PsiI TTA'TAA
Cuts at: 3760 3760
Size: 6190
PstI C_TGCA'G
Cuts at: 830 2459 3233 3448 3542 830
Size: 1629 774 215 94 3478
Fragments arranged by size:
3478 1629 774 215 94
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6190
PvuII CAG'CTG
Cuts at: 3234 3234
Size: 6190
SacI G_AGCT'C
Cuts at: 2120 2398 2120
Size: 278 5912
SalI G'TCGA_C
Cuts at: 1936 3532 1936
Size: 1596 4594
SapI GCTCTTCn'nnn_
Cuts at: 2559 2559
Size: 6190
SbfI CC_TGCA'GG
Cuts at: 2459 3542 2459
Size: 1083 5107
ScaI AGT'ACT
Cuts at: 4438 6150 4438
Size: 1712 4478
SfcI C'TryA_G
Cuts at: 826 1045 2455 3229 3444 3538 4673 5354 5545
Size: 219 1410 774 215 94 1135 681 191
Cuts at: 5545 826
Size: 1471
Fragments arranged by size:
1471 1410 1135 774 681 219 215 191
94
SfoI GGC'GCC
Cuts at: 3377 3377
Size: 6190
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6190
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6190
SmaI CCC'GGG
Cuts at: 1872 3456 3517 1872
Size: 1584 61 4545
Fragments arranged by size:
4545 1584 61
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3185 4294 5195 5457 820
Size: 197 926 1242 1109 901 262 1553
Fragments arranged by size:
1553 1242 1109 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3981 493
Size: 3488 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6190
SphI G_CATG'C
Cuts at: 1132 3371 3478 3548 1132
Size: 2239 107 70 3774
Fragments arranged by size:
3774 2239 107 70
SspI AAT'ATT
Cuts at: 5 52 4114 5
Size: 47 4062 2081
Fragments arranged by size:
4062 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6190
StyI C'CwwG_G
Cuts at: 513 1065 2004 2463 3629 513
Size: 552 939 459 1166 3074
Fragments arranged by size:
3074 1166 939 552 459
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3348 4217 4234 4387 4572 5017 325
Size: 1255 1768 869 17 153 185 445 1498
Fragments arranged by size:
1768 1498 1255 869 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2351 4436 6148
Size: 274 80 33 51 1443 374 2085 1712
Cuts at: 6148 96
Size: 138
Fragments arranged by size:
2085 1712 1443 374 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2937 2969 6150 6182 2937
Size: 32 3181 32 2945
Fragments arranged by size:
3181 2945 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6190
XbaI T'CTAG_A
Cuts at: 3526 3526
Size: 6190
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2209 2209
Size: 6190
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6190
XmaI C'CCGG_G
Cuts at: 1870 3454 3515 1870
Size: 1584 61 4545
Fragments arranged by size:
4545 1584 61
XmnI GAAnn'nnTTC
Cuts at: 3221 4319 3221
Size: 1098 5092
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3998 276
Size: 53 83 186 936 2464 2468
Fragments arranged by size:
2468 2464 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FseI
FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SrfI SwaI