Restriction map for
pFN21AB7269
(Circular) (Six-base) MAPSORT of: pFN21AB7269.seq Check: 9495 from: 1 to: 5998
pFN21AB7269 5998 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3808 278
Size: 53 83 186 936 2272 2468
Fragments arranged by size:
2468 2272 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3341 1937
Size: 1404 4594
Acc65I G'GTAC_C
Cuts at: 3319 3319
Size: 5998
AclI AA'CG_TT
Cuts at: 1563 2340 4125 4498 1563
Size: 777 1785 373 3063
Fragments arranged by size:
3063 1785 777 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2074 2313 2396 3093 4061 5445 5713 1632
Size: 442 239 83 697 968 1384 268 1917
Fragments arranged by size:
1917 1384 968 697 442 268 239 83
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5801
AflIII A'CryG_T
Cuts at: 1203 4897 5874 1203
Size: 3694 977 1327
Fragments arranged by size:
3694 1327 977
AgeI A'CCGG_T
Cuts at: 4829 4829
Size: 5998
AhdI GACnn_n'nnGTC
Cuts at: 4727 4727
Size: 5998
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5998
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5998
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2967 2999 2967
Size: 32 5966
AlwNI CAG_nnn'CTG
Cuts at: 3741 5313 3741
Size: 1572 4426
ApaI G_GGCC'C
Cuts at: 2198 2598 2198
Size: 400 5598
ApaLI G'TGCA_C
Cuts at: 4055 5211 4055
Size: 1156 4842
ApoI r'AATT_y
Cuts at: 1493 2829 3169 3307 3533 3685 5794 1493
Size: 1336 340 138 226 152 2109 1697
Fragments arranged by size:
2109 1697 1336 340 226 152 138
AseI AT'TA_AT
Cuts at: 160 1032 4552 160
Size: 872 3520 1606
Fragments arranged by size:
3520 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5998
AvaI C'yCGr_G
Cuts at: 1870 1943 3323 1870
Size: 73 1380 4545
Fragments arranged by size:
4545 1380 73
BamHI G'GATC_C
Cuts at: 2661 3095 3328 3695 2661
Size: 434 233 367 4964
Fragments arranged by size:
4964 434 367 233
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3319 4774 618
Size: 325 217 627 1532 1455 1842
Fragments arranged by size:
1842 1532 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2198 2598 3317 1391
Size: 807 400 719 4072
Fragments arranged by size:
4072 807 719 400
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3223 928
Size: 971 1324 3703
Fragments arranged by size:
3703 1324 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4189 4223 1903
Size: 34 2252 34 3678
Fragments arranged by size:
3678 2252 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3890 5100 3890
Size: 1210 4788
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5998
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4609 136
Size: 107 122 71 631 605 2937 1525
Fragments arranged by size:
2937 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5993 5993
Size: 5998
BlpI GC'TnA_GC
Cuts at: 3415 3415
Size: 5998
Bme1580I G_kGCm'C
Cuts at: 1790 2198 2598 4059 5215 1790
Size: 408 400 1461 1156 2573
Fragments arranged by size:
2573 1461 1156 408 400
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5998
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2511 2847 2931
Size: 416 53 83 399 24 1094 336 84
Cuts at: 2931 3049 3260 3729 4687 442
Size: 118 211 469 958 1753
Fragments arranged by size:
1753 1094 958 469 416 399 336 211
118 84 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5998
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2348 2780 2348
Size: 432 5566
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2103 2192 2691 3051 3074 4658 1239
Size: 864 89 499 360 23 1584 2579
Fragments arranged by size:
2579 1584 864 499 360 89 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4123 4988 5286 4123
Size: 865 298 4835
Fragments arranged by size:
4835 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4661 882
Size: 617 3162 2219
Fragments arranged by size:
3162 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3789 493
Size: 711 2585 2702
Fragments arranged by size:
2702 2585 711
BsaBI GATnn'nnATC
Cuts at: 5992 5992
Size: 5998
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3805 4187 4822 275
Size: 53 83 186 936 2272 382 635 1451
Fragments arranged by size:
2272 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4431 4829 5103 5250 5661 4431
Size: 398 274 147 411 4768
Fragments arranged by size:
4768 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5773
Fragments arranged by size:
5773 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2109 3052 3283 1186
Size: 923 943 231 3901
Fragments arranged by size:
3901 943 923 231
BseYI C'CCAG_C
Cuts at: 5201 5697 5201
Size: 496 5502
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5425
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3448 4209 5237 1540
Size: 275 186 1447 761 1028 2301
Fragments arranged by size:
2301 1447 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3062 3153 4059 4144 5215 3062
Size: 91 906 85 1071 3845
Fragments arranged by size:
3845 1071 906 91 85
BsmI GAATG_Cn'
Cuts at: 2319 3509 3602 2319
Size: 1190 93 4715
Fragments arranged by size:
4715 1190 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2198 2598 3062 3153 3317 4059 4144
Size: 399 408 400 464 91 164 742 85
Cuts at: 4144 5215 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 464 408 400 399 164
91 85
BspEI T'CCGG_A
Cuts at: 5661 5661
Size: 5998
BspHI T'CATG_A
Cuts at: 3887 5617 3887
Size: 1730 4268
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2161 2739 3353 844
Size: 1317 578 614 3489
Fragments arranged by size:
3489 1317 614 578
BsrBI CCG'CTC
Cuts at: 1394 1823 3885 1394
Size: 429 2062 3507
Fragments arranged by size:
3507 2062 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4493 4667 65
Size: 1183 3245 174 1396
Fragments arranged by size:
3245 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4642 4829 1700
Size: 110 2832 187 2869
Fragments arranged by size:
2869 2832 187 110
BsrGI T'GTAC_A
Cuts at: 96 2566 96
Size: 2470 3528
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5998
BssSI C'ACGA_G
Cuts at: 4058 5070 4058
Size: 1012 4986
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2314 794
Size: 1520 4478
BstBI TT'CG_AA
Cuts at: 4802 4802
Size: 5998
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5998
BstXI CCAn_nnnn'nTGG
Cuts at: 2205 2205
Size: 5998
BstYI r'GATC_y
Cuts at: 1972 2200 2661 3095 3328 3695 4080 4097 5549
Size: 228 461 434 233 367 385 17 1452
Cuts at: 5549 5635 5647 5993 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 461 434 385 367 346 233
228 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2189 513
Size: 552 1124 4322
Fragments arranged by size:
4322 1124 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5259
Fragments arranged by size:
5259 683 56
BtsI GCAGTG_nn'
Cuts at: 3514 4308 4328 3514
Size: 794 20 5184
Fragments arranged by size:
5184 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5998
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5963
DraI TTT'AAA
Cuts at: 2362 3302 2362
Size: 940 5058
DraIII CAC_nnn'GTG
Cuts at: 3007 3007
Size: 5998
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5005 809
Size: 507 3689 1802
Fragments arranged by size:
3689 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3445 4334 5963
Size: 54 1425 50 275 18 1615 889 1629
Cuts at: 5963 8
Size: 43
Fragments arranged by size:
1629 1615 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3445 1537
Size: 275 1633 4090
Fragments arranged by size:
4090 1633 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3928 1353
Size: 2575 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2012 4585 4959 5105 387
Size: 1064 138 423 2573 374 146 1280
Fragments arranged by size:
2573 1280 1064 423 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2074 2103 2192 2313 2396 2691 3051
Size: 393 442 29 89 121 83 295 360
Cuts at: 3051 3074 3093 4061 4658 5445 5713 1239
Size: 23 19 968 597 787 268 1524
Fragments arranged by size:
1524 968 787 597 442 393 360 295
268 121 89 83 29 23 19
EcoO109I rG'GnC_Cy
Cuts at: 2194 2594 2595 3028 2194
Size: 400 1 433 5164
Fragments arranged by size:
5164 433 400 1
EcoRI G'AATT_C
Cuts at: 2829 3307 3685 2829
Size: 478 378 5142
Fragments arranged by size:
5142 478 378
FspI TGC'GCA
Cuts at: 4504 4504
Size: 5998
HaeII r_GCGC'y
Cuts at: 1678 5145 1678
Size: 3467 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2374 2406 2638 2670 4639
Size: 32 135 32 802 32 232 32 1969
Cuts at: 4639 4671 4713 4745 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1969 802 232 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3342 3588 669
Size: 988 281 1404 246 3079
Fragments arranged by size:
3079 1404 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2474 2538 2790 748
Size: 1726 64 252 3956
Fragments arranged by size:
3956 1726 252 64
HpaI GTT'AAC
Cuts at: 3588 3588
Size: 5998
KpnI G_GTAC'C
Cuts at: 3323 3323
Size: 5998
MfeI C'AATT_G
Cuts at: 3597 3597
Size: 5998
MluI A'CGCG_T
Cuts at: 5874 5874
Size: 5998
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2765 5112 5296 1305
Size: 1460 2347 184 2007
Fragments arranged by size:
2347 2007 1460 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4176
Fragments arranged by size:
4176 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3956 4315 4474 518
Size: 648 2790 359 159 2042
Fragments arranged by size:
2790 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3414 4091 5239 5484 1606
Size: 1808 677 1148 245 2120
Fragments arranged by size:
2120 1808 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2189 513
Size: 552 1124 4322
Fragments arranged by size:
4322 1124 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5998
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5998
NotI GC'GGCC_GC
Cuts at: 3445 3445
Size: 5998
NspI r_CATG'y
Cuts at: 1132 1229 3146 3356 4901 1132
Size: 97 1917 210 1545 2229
Fragments arranged by size:
2229 1917 1545 210 97
PasI CC'CwG_GG
Cuts at: 1348 2205 1348
Size: 857 5141
PciI A'CATG_T
Cuts at: 4897 4897
Size: 5998
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5998
PfoI T'CCnGG_A
Cuts at: 2249 2249
Size: 5998
PmeI GTTT'AAAC
Cuts at: 3302 3302
Size: 5998
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4067 4099 5580 5612 4067
Size: 32 1481 32 4453
Fragments arranged by size:
4453 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3028 3028
Size: 5998
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5998
PsiI TTA'TAA
Cuts at: 2276 3568 2276
Size: 1292 4706
PspOMI G'GGCC_C
Cuts at: 2194 2594 2194
Size: 400 5598
PstI C_TGCA'G
Cuts at: 830 2736 3350 830
Size: 1906 614 3478
Fragments arranged by size:
3478 1906 614
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5998
SalI G'TCGA_C
Cuts at: 1936 3340 1936
Size: 1404 4594
SbfI CC_TGCA'GG
Cuts at: 3350 3350
Size: 5998
ScaI AGT'ACT
Cuts at: 4246 5958 4246
Size: 1712 4286
SfcI C'TryA_G
Cuts at: 826 1045 2732 3346 4481 5162 5353 826
Size: 219 1687 614 1135 681 191 1471
Fragments arranged by size:
1687 1471 1135 681 614 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5998
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5998
SmaI CCC'GGG
Cuts at: 1872 3325 1872
Size: 1453 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4102 5003 5265 820
Size: 197 926 2159 901 262 1553
Fragments arranged by size:
2159 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3789 493
Size: 3296 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5998
SphI G_CATG'C
Cuts at: 1132 3356 1132
Size: 2224 3774
SspI AAT'ATT
Cuts at: 5 52 3922 5
Size: 47 3870 2081
Fragments arranged by size:
3870 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5998
StyI C'CwwG_G
Cuts at: 513 1065 2074 2189 3031 3437 513
Size: 552 1009 115 842 406 3074
Fragments arranged by size:
3074 1009 842 552 406 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2897 4025 4042 4195 4380 4825 325
Size: 1255 1317 1128 17 153 185 445 1498
Fragments arranged by size:
1498 1317 1255 1128 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2566 4244 5956
Size: 274 80 33 51 1443 589 1678 1712
Cuts at: 5956 96
Size: 138
Fragments arranged by size:
1712 1678 1443 589 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5958 5990 5958
Size: 32 5966
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5998
XbaI T'CTAG_A
Cuts at: 3334 3334
Size: 5998
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5998
XmaI C'CCGG_G
Cuts at: 1870 3323 1870
Size: 1453 4545
XmnI GAAnn'nnTTC
Cuts at: 2456 2792 2960 3173 3202 4127 2456
Size: 336 168 213 29 925 4327
Fragments arranged by size:
4327 925 336 213 168 29
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3806 276
Size: 53 83 186 936 2272 2468
Fragments arranged by size:
2468 2272 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI