Restriction map for
pFN21AB7268
(Circular) (Six-base) MAPSORT of: pFN21AB7268.seq Check: 2339 from: 1 to: 6349
pFN21AB7268 6349 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2179 2179
Size: 6349
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4159 278
Size: 53 83 186 936 2623 2468
Fragments arranged by size:
2623 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3692 1937
Size: 1755 4594
Acc65I G'GTAC_C
Cuts at: 3670 3670
Size: 6349
AclI AA'CG_TT
Cuts at: 1563 4476 4849 1563
Size: 2913 373 3063
Fragments arranged by size:
3063 2913 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4412 5796 6064 1632
Size: 2780 1384 268 1917
Fragments arranged by size:
2780 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6152
AflIII A'CryG_T
Cuts at: 1203 3529 5248 6225 1203
Size: 2326 1719 977 1327
Fragments arranged by size:
2326 1719 1327 977
AgeI A'CCGG_T
Cuts at: 3182 5180 3182
Size: 1998 4351
AhdI GACnn_n'nnGTC
Cuts at: 3477 5078 3477
Size: 1601 4748
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6349
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2052 1184
Size: 868 5481
AlwNI CAG_nnn'CTG
Cuts at: 4092 5664 4092
Size: 1572 4777
ApaLI G'TGCA_C
Cuts at: 4406 5562 4406
Size: 1156 5193
ApoI r'AATT_y
Cuts at: 1493 2085 2586 2670 2818 3006 3174 3258 3342
Size: 592 501 84 148 188 168 84 84
Cuts at: 3342 3593 3658 3884 4036 6145 1493
Size: 251 65 226 152 2109 1697
Fragments arranged by size:
2109 1697 592 501 251 226 188 168
152 148 84 84 84 65
AseI AT'TA_AT
Cuts at: 160 1032 2658 4903 160
Size: 872 1626 2245 1606
Fragments arranged by size:
2245 1626 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6349
AvaI C'yCGr_G
Cuts at: 1870 1943 3674 1870
Size: 73 1731 4545
Fragments arranged by size:
4545 1731 73
BamHI G'GATC_C
Cuts at: 3679 4046 3679
Size: 367 5982
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2167 3670 5125 618
Size: 325 217 627 380 1503 1455 1842
Fragments arranged by size:
1842 1503 1455 627 380 325 217
BanII G_rGCy'C
Cuts at: 1391 2289 3236 3668 1391
Size: 898 947 432 4072
Fragments arranged by size:
4072 947 898 432
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2131 3476 928
Size: 971 232 1345 3801
Fragments arranged by size:
3801 1345 971 232
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4540 4574 1903
Size: 34 2603 34 3678
Fragments arranged by size:
3678 2603 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4241 5451 4241
Size: 1210 5139
BclI T'GATC_A
Cuts at: 1548 2523 1548
Size: 975 5374
BfrBI ATG'CAT
Cuts at: 2564 3032 3514 2564
Size: 468 482 5399
Fragments arranged by size:
5399 482 468
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4960 136
Size: 107 122 71 631 605 3288 1525
Fragments arranged by size:
3288 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6344 6344
Size: 6349
BlpI GC'TnA_GC
Cuts at: 3766 3766
Size: 6349
Bme1580I G_kGCm'C
Cuts at: 1790 4410 5566 1790
Size: 2620 1156 2573
Fragments arranged by size:
2620 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2197 1318
Size: 879 5470
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2240 4080 5038
Size: 416 53 83 399 24 823 1840 958
Cuts at: 5038 442
Size: 1753
Fragments arranged by size:
1840 1753 958 823 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6349
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2334 3288 5009 1239
Size: 1095 954 1721 2579
Fragments arranged by size:
2579 1721 1095 954
Bpu10I CC'TnA_GC
Cuts at: 2014 2014
Size: 6349
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2456 3227 3496 4474 5339 5637 2456
Size: 771 269 978 865 298 3168
Fragments arranged by size:
3168 978 865 771 298 269
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2201 2230 3322 5012 882
Size: 617 702 29 1092 1690 2219
Fragments arranged by size:
2219 1690 1092 702 617 29
BsaAI yAC'GTr
Cuts at: 493 1204 4140 493
Size: 711 2936 2702
Fragments arranged by size:
2936 2702 711
BsaBI GATnn'nnATC
Cuts at: 2525 6343 2525
Size: 3818 2531
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4156 4538 5173 275
Size: 53 83 186 936 2623 382 635 1451
Fragments arranged by size:
2623 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3182 4782 5180 5454 5601 6012 3182
Size: 1600 398 274 147 411 3519
Fragments arranged by size:
3519 1600 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6124
Fragments arranged by size:
6124 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6349
BseYI C'CCAG_C
Cuts at: 5552 6048 5552
Size: 496 5853
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5776
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3799 4560 5588 1540
Size: 275 186 1798 761 1028 2301
Fragments arranged by size:
2301 1798 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3236 4410 4495 5566 3236
Size: 1174 85 1071 4019
Fragments arranged by size:
4019 1174 1071 85
BsmI GAATG_Cn'
Cuts at: 2471 3516 3860 3953 2471
Size: 1045 344 93 4867
Fragments arranged by size:
4867 1045 344 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2187 2187
Size: 6349
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2289 3236 3668 4410 4495 5566 1391
Size: 399 499 947 432 742 85 1071 2174
Fragments arranged by size:
2174 1071 947 742 499 432 399 85
BspEI T'CCGG_A
Cuts at: 6012 6012
Size: 6349
BspHI T'CATG_A
Cuts at: 3481 4238 5968 3481
Size: 757 1730 3862
Fragments arranged by size:
3862 1730 757
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2179 3704 844
Size: 1335 1525 3489
Fragments arranged by size:
3489 1525 1335
BsrBI CCG'CTC
Cuts at: 1394 1823 4236 1394
Size: 429 2413 3507
Fragments arranged by size:
3507 2413 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3397 4844 5018 65
Size: 1183 2149 1447 174 1396
Fragments arranged by size:
2149 1447 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3182 4993 5180 1700
Size: 110 1372 1811 187 2869
Fragments arranged by size:
2869 1811 1372 187 110
BsrGI T'GTAC_A
Cuts at: 96 2187 3331 96
Size: 2091 1144 3114
Fragments arranged by size:
3114 2091 1144
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6349
BssSI C'ACGA_G
Cuts at: 4409 5421 4409
Size: 1012 5337
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6349
BstBI TT'CG_AA
Cuts at: 5153 5153
Size: 6349
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6349
BstXI CCAn_nnnn'nTGG
Cuts at: 3620 3620
Size: 6349
BstYI r'GATC_y
Cuts at: 1972 2979 3679 4046 4431 4448 5900 5986 5998
Size: 1007 700 367 385 17 1452 86 12
Cuts at: 5998 6344 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1007 700 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4858
Fragments arranged by size:
4858 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5610
Fragments arranged by size:
5610 683 56
BtsI GCAGTG_nn'
Cuts at: 2165 3865 4659 4679 2165
Size: 1700 794 20 3835
Fragments arranged by size:
3835 1700 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6349
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3025 3060 3193 3228 529
Size: 35 2461 35 133 35 3650
Fragments arranged by size:
3650 2461 133 35 35 35
DraI TTT'AAA
Cuts at: 2722 3653 2722
Size: 931 5418
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5356 809
Size: 507 4040 1802
Fragments arranged by size:
4040 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2130 3796 4685
Size: 54 1425 50 275 18 300 1666 889
Cuts at: 4685 6314 8
Size: 1629 43
Fragments arranged by size:
1666 1629 1425 889 300 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3796 1537
Size: 275 1984 4090
Fragments arranged by size:
4090 1984 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4279 1353
Size: 2926 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4936 5310 5456 387
Size: 1064 138 3347 374 146 1280
Fragments arranged by size:
3347 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3234 3234
Size: 6349
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2334 3288 4412 5009 5796 6064 1239
Size: 393 702 954 1124 597 787 268 1524
Fragments arranged by size:
1524 1124 954 787 702 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2068 3303 2068
Size: 1235 5114
EcoRI G'AATT_C
Cuts at: 3006 3174 3258 3342 3658 4036 3006
Size: 168 84 84 316 378 5319
Fragments arranged by size:
5319 378 316 168 84 84
FspI TGC'GCA
Cuts at: 4855 4855
Size: 6349
HaeII r_GCGC'y
Cuts at: 1678 5496 1678
Size: 3818 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2057 2089 2246 2278 2899
Size: 32 135 32 485 32 157 32 621
Cuts at: 2899 2931 2971 3003 3067 3099 3403 3435 3463
Size: 32 40 32 64 32 304 32 28
Cuts at: 3463 3495 4990 5022 5064 5096 1373
Size: 32 1495 32 42 32 2626
Fragments arranged by size:
2626 1495 621 485 304 157 135 64
42 40 32 32 32 32 32 32
32 32 32 32 32 28
HincII GTy'rAC
Cuts at: 669 1657 1938 3693 3939 669
Size: 988 281 1755 246 3079
Fragments arranged by size:
3079 1755 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6349
HpaI GTT'AAC
Cuts at: 3939 3939
Size: 6349
KpnI G_GTAC'C
Cuts at: 3674 3674
Size: 6349
MfeI C'AATT_G
Cuts at: 3407 3948 3407
Size: 541 5808
MluI A'CGCG_T
Cuts at: 6225 6225
Size: 6349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2184 5463 5647 1305
Size: 879 3279 184 2007
Fragments arranged by size:
3279 2007 879 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2132 10
Size: 54 1425 343 300 4227
Fragments arranged by size:
4227 1425 343 300 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2379 2398 2779 2847 3015 3183 3351
Size: 648 1213 19 381 68 168 168 168
Cuts at: 3351 3367 3618 4307 4666 4825 518
Size: 16 251 689 359 159 2042
Fragments arranged by size:
2042 1213 689 648 381 359 251 168
168 168 159 68 19 16
MspA1I CmG'CkG
Cuts at: 1606 3765 4442 5590 5835 1606
Size: 2159 677 1148 245 2120
Fragments arranged by size:
2159 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4858
Fragments arranged by size:
4858 939 552
NdeI CA'TA_TG
Cuts at: 387 2776 3364 3615 387
Size: 2389 588 251 3121
Fragments arranged by size:
3121 2389 588 251
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6349
NotI GC'GGCC_GC
Cuts at: 3796 3796
Size: 6349
NsiI A_TGCA'T
Cuts at: 2566 3034 3516 2566
Size: 468 482 5399
Fragments arranged by size:
5399 482 468
NspI r_CATG'y
Cuts at: 1132 1229 2378 2564 3036 3533 3707 5252 1132
Size: 97 1149 186 472 497 174 1545 2229
Fragments arranged by size:
2229 1545 1149 497 472 186 174 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6349
PciI A'CATG_T
Cuts at: 3529 5248 3529
Size: 1719 4630
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6349
PmeI GTTT'AAAC
Cuts at: 3653 3653
Size: 6349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4418 4450 5931 5963 4418
Size: 32 1481 32 4804
Fragments arranged by size:
4804 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2068 2068
Size: 6349
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6349
PsiI TTA'TAA
Cuts at: 3919 3919
Size: 6349
PstI C_TGCA'G
Cuts at: 830 3701 830
Size: 2871 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6349
SacI G_AGCT'C
Cuts at: 3236 3236
Size: 6349
SalI G'TCGA_C
Cuts at: 1936 3691 1936
Size: 1755 4594
SbfI CC_TGCA'GG
Cuts at: 3701 3701
Size: 6349
ScaI AGT'ACT
Cuts at: 4597 6309 4597
Size: 1712 4637
SexAI A'CCwGG_T
Cuts at: 2220 2220
Size: 6349
SfcI C'TryA_G
Cuts at: 826 1045 2212 3697 4832 5513 5704 826
Size: 219 1167 1485 1135 681 191 1471
Fragments arranged by size:
1485 1471 1167 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6349
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6349
SmaI CCC'GGG
Cuts at: 1872 3676 1872
Size: 1804 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2435 3242 3475 4453 5354 5616
Size: 197 926 492 807 233 978 901 262
Cuts at: 5616 820
Size: 1553
Fragments arranged by size:
1553 978 926 901 807 492 262 233
197
SnaBI TAC'GTA
Cuts at: 493 4140 493
Size: 3647 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6349
SphI G_CATG'C
Cuts at: 1132 2564 3036 3707 1132
Size: 1432 472 671 3774
Fragments arranged by size:
3774 1432 671 472
SspI AAT'ATT
Cuts at: 5 52 2755 4273 5
Size: 47 2703 1518 2081
Fragments arranged by size:
2703 2081 1518 47
StuI AGG'CCT
Cuts at: 1517 2319 2717 2801 2885 2969 3221 1517
Size: 802 398 84 84 84 252 4645
Fragments arranged by size:
4645 802 398 252 84 84 84
StyI C'CwwG_G
Cuts at: 513 1065 2004 2104 2352 3788 513
Size: 552 939 100 248 1436 3074
Fragments arranged by size:
3074 1436 939 552 248 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4376 4393 4546 4731 5176 325
Size: 1255 2796 17 153 185 445 1498
Fragments arranged by size:
2796 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2187 2995 3331
Size: 274 80 33 51 1443 210 808 336
Cuts at: 3331 4595 6307 96
Size: 1264 1712 138
Fragments arranged by size:
1712 1443 1264 808 336 274 210 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6309 6341 6309
Size: 32 6317
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6349
XbaI T'CTAG_A
Cuts at: 3685 3685
Size: 6349
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6349
XmaI C'CCGG_G
Cuts at: 1870 3674 1870
Size: 1804 4545
XmnI GAAnn'nnTTC
Cuts at: 2361 4478 2361
Size: 2117 4232
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4157 276
Size: 53 83 186 936 2623 2468
Fragments arranged by size:
2623 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SapI SfiI SfoI SrfI SwaI XcmI