Restriction map for
pFN21AB7267
(Circular) (Six-base) MAPSORT of: pFN21AB7267.seq Check: 1429 from: 1 to: 5668
pFN21AB7267 5668 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2761 2761
Size: 5668
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3478 278
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3011 1937
Size: 1074 4594
Acc65I G'GTAC_C
Cuts at: 2218 2989 2218
Size: 771 4897
AclI AA'CG_TT
Cuts at: 1563 3795 4168 1563
Size: 2232 373 3063
Fragments arranged by size:
3063 2232 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3731 5115 5383 1632
Size: 2099 1384 268 1917
Fragments arranged by size:
2099 1917 1384 268
AfeI AGC'GCT
Cuts at: 2918 2918
Size: 5668
AflII C'TTAA_G
Cuts at: 820 1017 2072 820
Size: 197 1055 4416
Fragments arranged by size:
4416 1055 197
AflIII A'CryG_T
Cuts at: 1203 2593 4567 5544 1203
Size: 1390 1974 977 1327
Fragments arranged by size:
1974 1390 1327 977
AgeI A'CCGG_T
Cuts at: 2221 4499 2221
Size: 2278 3390
AhdI GACnn_n'nnGTC
Cuts at: 4397 4397
Size: 5668
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5668
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5668
AlwNI CAG_nnn'CTG
Cuts at: 2800 3411 4983 2800
Size: 611 1572 3485
Fragments arranged by size:
3485 1572 611
ApaI G_GGCC'C
Cuts at: 2423 2423
Size: 5668
ApaLI G'TGCA_C
Cuts at: 2732 3725 4881 2732
Size: 993 1156 3519
Fragments arranged by size:
3519 1156 993
ApoI r'AATT_y
Cuts at: 1493 2977 3203 3355 5464 1493
Size: 1484 226 152 2109 1697
Fragments arranged by size:
2109 1697 1484 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4222 160
Size: 872 3190 1606
Fragments arranged by size:
3190 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
AvaI C'yCGr_G
Cuts at: 1870 1943 2024 2153 2301 2309 2564 2993 1870
Size: 73 81 129 148 8 255 429 4545
Fragments arranged by size:
4545 429 255 148 129 81 73 8
AvrII C'CTAG_G
Cuts at: 2344 2633 2344
Size: 289 5379
BamHI G'GATC_C
Cuts at: 2998 3365 2998
Size: 367 5301
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2218 2224 2230 2245 2305
Size: 325 217 627 431 6 6 15 60
Cuts at: 2305 2410 2773 2989 4444 618
Size: 105 363 216 1455 1842
Fragments arranged by size:
1842 1455 627 431 363 325 217 216
105 60 15 6 6
BanII G_rGCy'C
Cuts at: 1391 2160 2300 2423 2987 1391
Size: 769 140 123 564 4072
Fragments arranged by size:
4072 769 564 140 123
BbeI G_GCGC'C
Cuts at: 2249 2309 2249
Size: 60 5608
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2048 928
Size: 971 149 4548
Fragments arranged by size:
4548 971 149
BbvCI CC'TCA_GC
Cuts at: 2894 2894
Size: 5668
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2639 2673 2930 2964 3859 3893 1903
Size: 34 702 34 257 34 895 34 3678
Fragments arranged by size:
3678 895 702 257 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3560 4770 3560
Size: 1210 4458
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5668
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2107 2756 4279
Size: 107 122 71 631 605 435 649 1523
Cuts at: 4279 136
Size: 1525
Fragments arranged by size:
1525 1523 649 631 605 435 122 107
71
BglII A'GATC_T
Cuts at: 5663 5663
Size: 5668
BlpI GC'TnA_GC
Cuts at: 3085 3085
Size: 5668
Bme1580I G_kGCm'C
Cuts at: 1790 2273 2423 2736 3729 4885 1790
Size: 483 150 313 993 1156 2573
Fragments arranged by size:
2573 1156 993 483 313 150
BmgBI CAC'GTC
Cuts at: 1318 2948 1318
Size: 1630 4038
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2234 3399 4357
Size: 416 53 83 399 24 817 1165 958
Cuts at: 4357 442
Size: 1753
Fragments arranged by size:
1753 1165 958 817 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5668
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4328 1239
Size: 3089 2579
Bpu10I CC'TnA_GC
Cuts at: 2762 2894 2762
Size: 132 5536
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2066 3793 4658 4956 2066
Size: 1727 865 298 2778
Fragments arranged by size:
2778 1727 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2320 4331 882
Size: 617 821 2011 2219
Fragments arranged by size:
2219 2011 821 617
BsaAI yAC'GTr
Cuts at: 493 1204 2596 3459 493
Size: 711 1392 863 2702
Fragments arranged by size:
2702 1392 863 711
BsaBI GATnn'nnATC
Cuts at: 5662 5662
Size: 5668
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2246 2306 3475 3857
Size: 53 83 186 936 713 60 1169 382
Cuts at: 3857 4492 275
Size: 635 1451
Fragments arranged by size:
1451 1169 936 713 635 382 186 83
60 53
BsaWI w'CCGG_w
Cuts at: 2221 4101 4499 4773 4920 5331 2221
Size: 1880 398 274 147 411 2558
Fragments arranged by size:
2558 1880 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5443
Fragments arranged by size:
5443 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2656 1186
Size: 1470 4198
BseYI C'CCAG_C
Cuts at: 2509 4871 5367 2509
Size: 2362 496 2810
Fragments arranged by size:
2810 2362 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2905 2933 1163
Size: 573 1169 28 3898
Fragments arranged by size:
3898 1169 573 28
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2253 2645 3118 3879 4907 1540
Size: 275 186 252 392 473 761 1028 2301
Fragments arranged by size:
2301 1028 761 473 392 275 252 186
BsiHKAI G_wGCw'C
Cuts at: 2736 3729 3814 4885 2736
Size: 993 85 1071 3519
Fragments arranged by size:
3519 1071 993 85
BsmI GAATG_Cn'
Cuts at: 3179 3272 3179
Size: 93 5575
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2160 2273 2300 2423 2736 2987 3729
Size: 399 370 113 27 123 313 251 742
Cuts at: 3729 3814 4885 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 370 313 251 123
113 85 27
BspEI T'CCGG_A
Cuts at: 5331 5331
Size: 5668
BspHI T'CATG_A
Cuts at: 3557 5287 3557
Size: 1730 3938
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2761 3023 844
Size: 1917 262 3489
Fragments arranged by size:
3489 1917 262
BsrBI CCG'CTC
Cuts at: 1394 1823 2722 3555 1394
Size: 429 899 833 3507
Fragments arranged by size:
3507 899 833 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4163 4337 65
Size: 1183 2915 174 1396
Fragments arranged by size:
2915 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2101 2209 2221 2227 2248 4312 4499
Size: 110 291 108 12 6 21 2064 187
Cuts at: 4499 1700
Size: 2869
Fragments arranged by size:
2869 2064 291 187 110 108 21 12
6
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5668
BssHII G'CGCG_C
Cuts at: 1925 2110 2486 1925
Size: 185 376 5107
Fragments arranged by size:
5107 376 185
BssSI C'ACGA_G
Cuts at: 3728 4740 3728
Size: 1012 4656
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5668
BstBI TT'CG_AA
Cuts at: 4472 4472
Size: 5668
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5668
BstXI CCAn_nnnn'nTGG
Cuts at: 2414 2414
Size: 5668
BstYI r'GATC_y
Cuts at: 1972 2998 3365 3750 3767 5219 5305 5317 5663
Size: 1026 367 385 17 1452 86 12 346
Cuts at: 5663 1972
Size: 1977
Fragments arranged by size:
1977 1452 1026 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2819 2819
Size: 5668
BtgI C'CryG_G
Cuts at: 513 1065 2036 513
Size: 552 971 4145
Fragments arranged by size:
4145 971 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4929
Fragments arranged by size:
4929 683 56
BtsI GCAGTG_nn'
Cuts at: 2358 3184 3978 3998 2358
Size: 826 794 20 4028
Fragments arranged by size:
4028 826 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5668
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5633
DraI TTT'AAA
Cuts at: 2972 2972
Size: 5668
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4675 809
Size: 507 3359 1802
Fragments arranged by size:
3359 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2250 2811 3115
Size: 54 1425 50 275 18 420 561 304
Cuts at: 3115 4004 5633 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 561 420 304 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2250 3115 1537
Size: 275 438 865 4090
Fragments arranged by size:
4090 865 438 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3598 1353
Size: 2245 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4255 4629 4775 387
Size: 1064 138 2666 374 146 1280
Fragments arranged by size:
2666 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3731 4328 5115 5383 1239
Size: 393 2099 597 787 268 1524
Fragments arranged by size:
2099 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2193 2419 2193
Size: 226 5442
EcoRI G'AATT_C
Cuts at: 2977 3355 2977
Size: 378 5290
FspI TGC'GCA
Cuts at: 2476 4174 2476
Size: 1698 3970
FspAI rTGC'GCAy
Cuts at: 2476 2476
Size: 5668
HaeII r_GCGC'y
Cuts at: 1678 2153 2249 2309 2525 2762 2920 4815 1678
Size: 475 96 60 216 237 158 1895 2531
Fragments arranged by size:
2531 1895 475 237 216 158 96 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4309 4341 4383 4415 1373
Size: 32 135 32 2737 32 42 32 2626
Fragments arranged by size:
2737 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3012 3258 669
Size: 988 281 1074 246 3079
Fragments arranged by size:
3079 1074 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5668
HpaI GTT'AAC
Cuts at: 3258 3258
Size: 5668
KasI G'GCGC_C
Cuts at: 2245 2305 2245
Size: 60 5608
KpnI G_GTAC'C
Cuts at: 2222 2993 2222
Size: 771 4897
MfeI C'AATT_G
Cuts at: 3267 3267
Size: 5668
MluI A'CGCG_T
Cuts at: 5544 5544
Size: 5668
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2158 2343 4782 4966 1305
Size: 853 185 2439 184 2007
Fragments arranged by size:
2439 2007 853 185 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2813 10
Size: 54 1425 343 981 2865
Fragments arranged by size:
2865 1425 981 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3626 3985 4144 518
Size: 648 2460 359 159 2042
Fragments arranged by size:
2460 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3084 3761 4909 5154 1606
Size: 1478 677 1148 245 2120
Fragments arranged by size:
2120 1478 1148 677 245
NaeI GCC'GGC
Cuts at: 2103 2250 2103
Size: 147 5521
NarI GG'CG_CC
Cuts at: 2246 2306 2246
Size: 60 5608
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5116
NdeI CA'TA_TG
Cuts at: 387 2788 387
Size: 2401 3267
NgoMIV G'CCGG_C
Cuts at: 2101 2248 2101
Size: 147 5521
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5668
NotI GC'GGCC_GC
Cuts at: 3115 3115
Size: 5668
NspI r_CATG'y
Cuts at: 1132 1229 3026 4571 1132
Size: 97 1797 1545 2229
Fragments arranged by size:
2229 1797 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5668
PciI A'CATG_T
Cuts at: 4567 4567
Size: 5668
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5668
PfoI T'CCnGG_A
Cuts at: 2439 2439
Size: 5668
PmeI GTTT'AAAC
Cuts at: 2972 2972
Size: 5668
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3737 3769 5250 5282 3737
Size: 32 1481 32 4123
Fragments arranged by size:
4123 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2193 2193
Size: 5668
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5668
PsiI TTA'TAA
Cuts at: 3238 3238
Size: 5668
PspOMI G'GGCC_C
Cuts at: 2419 2419
Size: 5668
PspXI vC'TCGA_Gb
Cuts at: 2024 2024
Size: 5668
PstI C_TGCA'G
Cuts at: 830 2208 2688 2937 3020 830
Size: 1378 480 249 83 3478
Fragments arranged by size:
3478 1378 480 249 83
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5668
SalI G'TCGA_C
Cuts at: 1936 3010 1936
Size: 1074 4594
SbfI CC_TGCA'GG
Cuts at: 2937 3020 2937
Size: 83 5585
ScaI AGT'ACT
Cuts at: 3916 5628 3916
Size: 1712 3956
SfcI C'TryA_G
Cuts at: 826 1045 2204 2684 2872 2933 3016 4151 4832
Size: 219 1159 480 188 61 83 1135 681
Cuts at: 4832 5023 826
Size: 191 1471
Fragments arranged by size:
1471 1159 1135 681 480 219 191 188
83 61
SfoI GGC'GCC
Cuts at: 2247 2307 2247
Size: 60 5608
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5668
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5668
SmaI CCC'GGG
Cuts at: 1872 2566 2995 1872
Size: 694 429 4545
Fragments arranged by size:
4545 694 429
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2024 2072 2081 3772 4673 4935
Size: 197 926 81 48 9 1691 901 262
Cuts at: 4935 820
Size: 1553
Fragments arranged by size:
1691 1553 926 901 262 197 81 48
9
SnaBI TAC'GTA
Cuts at: 493 3459 493
Size: 2966 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5668
SphI G_CATG'C
Cuts at: 1132 3026 1132
Size: 1894 3774
SspI AAT'ATT
Cuts at: 5 52 3592 5
Size: 47 3540 2081
Fragments arranged by size:
3540 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5668
StyI C'CwwG_G
Cuts at: 513 1065 2344 2633 3107 513
Size: 552 1279 289 474 3074
Fragments arranged by size:
3074 1279 552 474 289
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3695 3712 3865 4050 4495 325
Size: 1255 2115 17 153 185 445 1498
Fragments arranged by size:
2115 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3914 5626 96
Size: 274 80 33 51 1443 1937 1712 138
Fragments arranged by size:
1937 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2021 2053 2421 2453 2603 2635 5628 5660 2021
Size: 32 368 32 150 32 2993 32 2029
Fragments arranged by size:
2993 2029 368 150 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5668
XbaI T'CTAG_A
Cuts at: 3004 3004
Size: 5668
XhoI C'TCGA_G
Cuts at: 1943 2024 1943
Size: 81 5587
XmaI C'CCGG_G
Cuts at: 1870 2564 2993 1870
Size: 694 429 4545
Fragments arranged by size:
4545 694 429
XmnI GAAnn'nnTTC
Cuts at: 3797 3797
Size: 5668
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3476 276
Size: 53 83 186 936 1942 2468
Fragments arranged by size:
2468 1942 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BfrBI BplI BsiWI BsmBI BstZ17I
DraIII EcoICRI EcoNI EcoRV FalI FseI NruI NsiI
PacI PmlI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SrfI SwaI XcmI