Restriction map for
pFN21AB7265
(Circular) (Six-base) MAPSORT of: pFN21AB7265.seq Check: 1274 from: 1 to: 5761
pFN21AB7265 5761 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2677 3025 2677
Size: 348 5413
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3571 278
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3104 1937
Size: 1167 4594
Acc65I G'GTAC_C
Cuts at: 2675 3082 2675
Size: 407 5354
AclI AA'CG_TT
Cuts at: 1563 3888 4261 1563
Size: 2325 373 3063
Fragments arranged by size:
3063 2325 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2378 2669 2973 3824 5208 5476 1632
Size: 746 291 304 851 1384 268 1917
Fragments arranged by size:
1917 1384 851 746 304 291 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5564
AflIII A'CryG_T
Cuts at: 1203 4660 5637 1203
Size: 3457 977 1327
Fragments arranged by size:
3457 1327 977
AgeI A'CCGG_T
Cuts at: 4592 4592
Size: 5761
AhdI GACnn_n'nnGTC
Cuts at: 4490 4490
Size: 5761
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5761
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2715 1184
Size: 1531 4230
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2012 2044 2012
Size: 32 5729
AlwNI CAG_nnn'CTG
Cuts at: 2572 3504 5076 2572
Size: 932 1572 3257
Fragments arranged by size:
3257 1572 932
ApaLI G'TGCA_C
Cuts at: 3818 4974 3818
Size: 1156 4605
ApoI r'AATT_y
Cuts at: 1493 3070 3296 3448 5557 1493
Size: 1577 226 152 2109 1697
Fragments arranged by size:
2109 1697 1577 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4315 160
Size: 872 3283 1606
Fragments arranged by size:
3283 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
AvaI C'yCGr_G
Cuts at: 1870 1943 3086 1870
Size: 73 1143 4545
Fragments arranged by size:
4545 1143 73
BamHI G'GATC_C
Cuts at: 3091 3458 3091
Size: 367 5394
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2456 2675 2719 3082 4537
Size: 325 217 627 669 219 44 363 1455
Cuts at: 4537 618
Size: 1842
Fragments arranged by size:
1842 1455 669 627 363 325 219 217
44
BanII G_rGCy'C
Cuts at: 1391 2895 3080 1391
Size: 1504 185 4072
Fragments arranged by size:
4072 1504 185
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2565 928
Size: 971 666 4124
Fragments arranged by size:
4124 971 666
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3952 3986 1903
Size: 34 2015 34 3678
Fragments arranged by size:
3678 2015 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3653 4863 3653
Size: 1210 4551
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5761
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4372 136
Size: 107 122 71 631 605 2700 1525
Fragments arranged by size:
2700 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2529 5756 2529
Size: 3227 2534
BlpI GC'TnA_GC
Cuts at: 2039 2510 2889 3178 2039
Size: 471 379 289 4622
Fragments arranged by size:
4622 471 379 289
Bme1580I G_kGCm'C
Cuts at: 1790 3822 4978 1790
Size: 2032 1156 2573
Fragments arranged by size:
2573 2032 1156
BmgBI CAC'GTC
Cuts at: 1318 2845 1318
Size: 1527 4234
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2950 3492 4450
Size: 416 53 83 399 24 1533 542 958
Cuts at: 4450 442
Size: 1753
Fragments arranged by size:
1753 1533 958 542 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5761
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2429 2936 4421 1239
Size: 1190 507 1485 2579
Fragments arranged by size:
2579 1485 1190 507
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3886 4751 5049 3886
Size: 865 298 4598
Fragments arranged by size:
4598 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4424 882
Size: 617 2925 2219
Fragments arranged by size:
2925 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3552 493
Size: 711 2348 2702
Fragments arranged by size:
2702 2348 711
BsaBI GATnn'nnATC
Cuts at: 5755 5755
Size: 5761
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2546 3568 3950 4585
Size: 53 83 186 936 1013 1022 382 635
Cuts at: 4585 275
Size: 1451
Fragments arranged by size:
1451 1022 1013 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2275 2928 4194 4592 4866 5013 5424 2275
Size: 653 1266 398 274 147 411 2612
Fragments arranged by size:
2612 1266 653 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2012 2042 2807 2837 1178
Size: 30 165 30 609 30 765 30 4102
Fragments arranged by size:
4102 765 609 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2651 2820 1186
Size: 1465 169 4127
Fragments arranged by size:
4127 1465 169
BseYI C'CCAG_C
Cuts at: 2611 2854 2981 4964 5460 2611
Size: 243 127 1983 496 2912
Fragments arranged by size:
2912 1983 496 243 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2711 1163
Size: 573 975 4213
Fragments arranged by size:
4213 975 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3211 3972 5000 1540
Size: 275 186 1210 761 1028 2301
Fragments arranged by size:
2301 1210 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2895 2940 3822 3907 4978 2895
Size: 45 882 85 1071 3678
Fragments arranged by size:
3678 1071 882 85 45
BsmI GAATG_Cn'
Cuts at: 2867 3272 3365 2867
Size: 405 93 5263
Fragments arranged by size:
5263 405 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2895 2940 3080 3822 3907 4978 1391
Size: 399 1105 45 140 742 85 1071 2174
Fragments arranged by size:
2174 1105 1071 742 399 140 85 45
BspEI T'CCGG_A
Cuts at: 2275 2928 5424 2275
Size: 653 2496 2612
Fragments arranged by size:
2612 2496 653
BspHI T'CATG_A
Cuts at: 3650 5380 3650
Size: 1730 4031
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2677 3025 3116 844
Size: 1833 348 91 3489
Fragments arranged by size:
3489 1833 348 91
BsrBI CCG'CTC
Cuts at: 1394 1823 3648 1394
Size: 429 1825 3507
Fragments arranged by size:
3507 1825 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4256 4430 65
Size: 1183 3008 174 1396
Fragments arranged by size:
3008 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2195 2493 4405 4592 1700
Size: 110 385 298 1912 187 2869
Fragments arranged by size:
2869 1912 385 298 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5761
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5761
BssSI C'ACGA_G
Cuts at: 3821 4833 3821
Size: 1012 4749
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5761
BstBI TT'CG_AA
Cuts at: 4565 4565
Size: 5761
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5761
BstYI r'GATC_y
Cuts at: 1972 2529 3091 3458 3843 3860 5312 5398 5410
Size: 557 562 367 385 17 1452 86 12
Cuts at: 5410 5756 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 562 557 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4270
Fragments arranged by size:
4270 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5022
Fragments arranged by size:
5022 683 56
BtsI GCAGTG_nn'
Cuts at: 2473 3277 4071 4091 2473
Size: 804 794 20 4143
Fragments arranged by size:
4143 804 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5761
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5726
DraI TTT'AAA
Cuts at: 3065 3065
Size: 5761
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4768 809
Size: 507 3452 1802
Fragments arranged by size:
3452 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2764 3208 4097
Size: 54 1425 50 275 18 934 444 889
Cuts at: 4097 5726 8
Size: 1629 43
Fragments arranged by size:
1629 1425 934 889 444 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3208 1537
Size: 275 1396 4090
Fragments arranged by size:
4090 1396 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3691 1353
Size: 2338 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4348 4722 4868 387
Size: 1064 138 2759 374 146 1280
Fragments arranged by size:
2759 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2893 2893
Size: 5761
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2378 2429 2669 2936 2973 3824 4421
Size: 393 746 51 240 267 37 851 597
Cuts at: 4421 5208 5476 1239
Size: 787 268 1524
Fragments arranged by size:
1524 851 787 746 597 393 268 267
240 51 37
EcoNI CCTnn'n_nnAGG
Cuts at: 2297 2357 2297
Size: 60 5701
EcoO109I rG'GnC_Cy
Cuts at: 2920 2971 2920
Size: 51 5710
EcoRI G'AATT_C
Cuts at: 3070 3448 3070
Size: 378 5383
EcoRV GAT'ATC
Cuts at: 2587 2587
Size: 5761
FspI TGC'GCA
Cuts at: 4267 4267
Size: 5761
HaeII r_GCGC'y
Cuts at: 1678 2487 4908 1678
Size: 809 2421 2531
Fragments arranged by size:
2531 2421 809
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2136 2168 2200 2807 2839
Size: 32 135 32 564 32 32 607 32
Cuts at: 2839 2942 2974 4402 4434 4476 4508 1373
Size: 103 32 1428 32 42 32 2626
Fragments arranged by size:
2626 1428 607 564 135 103 42 32
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3105 3351 669
Size: 988 281 1167 246 3079
Fragments arranged by size:
3079 1167 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5761
HpaI GTT'AAC
Cuts at: 3351 3351
Size: 5761
KpnI G_GTAC'C
Cuts at: 2679 3086 2679
Size: 407 5354
MfeI C'AATT_G
Cuts at: 3360 3360
Size: 5761
MluI A'CGCG_T
Cuts at: 5637 5637
Size: 5761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2751 4875 5059 1305
Size: 1446 2124 184 2007
Fragments arranged by size:
2124 2007 1446 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2766 10
Size: 54 1425 343 934 3005
Fragments arranged by size:
3005 1425 934 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3719 4078 4237 518
Size: 648 2553 359 159 2042
Fragments arranged by size:
2553 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2490 3177 3854 5002 5247 1606
Size: 884 687 677 1148 245 2120
Fragments arranged by size:
2120 1148 884 687 677 245
NaeI GCC'GGC
Cuts at: 2495 2495
Size: 5761
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4270
Fragments arranged by size:
4270 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5761
NgoMIV G'CCGG_C
Cuts at: 2493 2493
Size: 5761
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5761
NotI GC'GGCC_GC
Cuts at: 3208 3208
Size: 5761
NspI r_CATG'y
Cuts at: 1132 1229 2107 3119 4664 1132
Size: 97 878 1012 1545 2229
Fragments arranged by size:
2229 1545 1012 878 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5761
PciI A'CATG_T
Cuts at: 4660 4660
Size: 5761
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5761
PmeI GTTT'AAAC
Cuts at: 3065 3065
Size: 5761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2012 2044 3830 3862 5343 5375 2012
Size: 32 1786 32 1481 32 2398
Fragments arranged by size:
2398 1786 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2920 2971 2920
Size: 51 5710
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5761
PsiI TTA'TAA
Cuts at: 3331 3331
Size: 5761
PstI C_TGCA'G
Cuts at: 830 3113 830
Size: 2283 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5761
PvuII CAG'CTG
Cuts at: 2490 2490
Size: 5761
SacI G_AGCT'C
Cuts at: 2895 2895
Size: 5761
SalI G'TCGA_C
Cuts at: 1936 3103 1936
Size: 1167 4594
SanDI GG'GwC_CC
Cuts at: 2971 2971
Size: 5761
SbfI CC_TGCA'GG
Cuts at: 3113 3113
Size: 5761
ScaI AGT'ACT
Cuts at: 4009 5721 4009
Size: 1712 4049
SfcI C'TryA_G
Cuts at: 826 1045 2836 3109 4244 4925 5116 826
Size: 219 1791 273 1135 681 191 1471
Fragments arranged by size:
1791 1471 1135 681 273 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5761
SmaI CCC'GGG
Cuts at: 1872 3088 1872
Size: 1216 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3865 4766 5028 820
Size: 197 926 1922 901 262 1553
Fragments arranged by size:
1922 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3552 493
Size: 3059 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5761
SphI G_CATG'C
Cuts at: 1132 3119 1132
Size: 1987 3774
SspI AAT'ATT
Cuts at: 5 52 3685 5
Size: 47 3633 2081
Fragments arranged by size:
3633 2081 47
StuI AGG'CCT
Cuts at: 1517 2834 1517
Size: 1317 4444
StyI C'CwwG_G
Cuts at: 513 1065 2004 2082 2298 2896 3021 3200 513
Size: 552 939 78 216 598 125 179 3074
Fragments arranged by size:
3074 939 598 552 216 179 125 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2846 3788 3805 3958 4143 4588 325
Size: 1255 1266 942 17 153 185 445 1498
Fragments arranged by size:
1498 1266 1255 942 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4007 5719 96
Size: 274 80 33 51 1443 2030 1712 138
Fragments arranged by size:
2030 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5721 5753 5721
Size: 32 5729
Tth111I GACn'n_nGTC
Cuts at: 1316 2924 1316
Size: 1608 4153
XbaI T'CTAG_A
Cuts at: 3097 3097
Size: 5761
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5761
XmaI C'CCGG_G
Cuts at: 1870 3086 1870
Size: 1216 4545
XmnI GAAnn'nnTTC
Cuts at: 2024 2531 3890 2024
Size: 507 1359 3895
Fragments arranged by size:
3895 1359 507
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3569 276
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacII SapI
SexAI SfiI SfoI SrfI SwaI XcmI