Restriction map for
pFN21AB7263
(Circular) (Six-base) MAPSORT of: pFN21AB7263.seq Check: 7918 from: 1 to: 5650
pFN21AB7263 5650 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3460 278
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2237 2993 1937
Size: 300 756 4594
Fragments arranged by size:
4594 756 300
Acc65I G'GTAC_C
Cuts at: 2971 2971
Size: 5650
AclI AA'CG_TT
Cuts at: 1563 3777 4150 1563
Size: 2214 373 3063
Fragments arranged by size:
3063 2214 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3713 5097 5365 1632
Size: 2081 1384 268 1917
Fragments arranged by size:
2081 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5453
AflIII A'CryG_T
Cuts at: 1203 2729 4549 5526 1203
Size: 1526 1820 977 1327
Fragments arranged by size:
1820 1526 1327 977
AgeI A'CCGG_T
Cuts at: 4481 4481
Size: 5650
AhdI GACnn_n'nnGTC
Cuts at: 4379 4379
Size: 5650
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5650
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2314 1184
Size: 1130 4520
AlwNI CAG_nnn'CTG
Cuts at: 3393 4965 3393
Size: 1572 4078
ApaLI G'TGCA_C
Cuts at: 3707 4863 3707
Size: 1156 4494
ApoI r'AATT_y
Cuts at: 1493 2916 2959 3185 3337 5446 1493
Size: 1423 43 226 152 2109 1697
Fragments arranged by size:
2109 1697 1423 226 152 43
AseI AT'TA_AT
Cuts at: 160 1032 4204 160
Size: 872 3172 1606
Fragments arranged by size:
3172 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
AvaI C'yCGr_G
Cuts at: 1870 1943 2975 1870
Size: 73 1032 4545
Fragments arranged by size:
4545 1032 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2718 2751 2718
Size: 33 5617
BamHI G'GATC_C
Cuts at: 2980 3347 2980
Size: 367 5283
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2971 4426 618
Size: 325 217 627 1184 1455 1842
Fragments arranged by size:
1842 1455 1184 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2040 2969 1391
Size: 649 929 4072
Fragments arranged by size:
4072 929 649
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4679
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3841 3875 1903
Size: 34 1904 34 3678
Fragments arranged by size:
3678 1904 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2746 3542 4752 2746
Size: 796 1210 3644
Fragments arranged by size:
3644 1210 796
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5650
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4261 136
Size: 107 122 71 631 605 2589 1525
Fragments arranged by size:
2589 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5645 5645
Size: 5650
BlpI GC'TnA_GC
Cuts at: 2307 3067 2307
Size: 760 4890
Bme1580I G_kGCm'C
Cuts at: 1790 3711 4867 1790
Size: 1921 1156 2573
Fragments arranged by size:
2573 1921 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5650
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2858 3381 4339
Size: 416 53 83 399 24 1441 523 958
Cuts at: 4339 442
Size: 1753
Fragments arranged by size:
1753 1441 958 523 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5650
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2090 2262 2360 4310 1239
Size: 851 172 98 1950 2579
Fragments arranged by size:
2579 1950 851 172 98
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3775 4640 4938 3775
Size: 865 298 4487
Fragments arranged by size:
4487 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4313 882
Size: 617 2814 2219
Fragments arranged by size:
2814 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3441 493
Size: 711 2237 2702
Fragments arranged by size:
2702 2237 711
BsaBI GATnn'nnATC
Cuts at: 5644 5644
Size: 5650
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3457 3839 4474 275
Size: 53 83 186 936 1924 382 635 1451
Fragments arranged by size:
1924 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4083 4481 4755 4902 5313 4083
Size: 398 274 147 411 4420
Fragments arranged by size:
4420 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5425
Fragments arranged by size:
5425 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2956 1186
Size: 1770 3880
BseYI C'CCAG_C
Cuts at: 4853 5349 4853
Size: 496 5154
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2384 1163
Size: 573 648 4429
Fragments arranged by size:
4429 648 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3100 3861 4889 1540
Size: 275 186 1099 761 1028 2301
Fragments arranged by size:
2301 1099 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3711 3796 4867 3711
Size: 85 1071 4494
Fragments arranged by size:
4494 1071 85
BsmI GAATG_Cn'
Cuts at: 3161 3254 3161
Size: 93 5557
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2969 3711 3796 4867 1391
Size: 399 250 929 742 85 1071 2174
Fragments arranged by size:
2174 1071 929 742 399 250 85
BspEI T'CCGG_A
Cuts at: 5313 5313
Size: 5650
BspHI T'CATG_A
Cuts at: 3539 5269 3539
Size: 1730 3920
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3005 844
Size: 2161 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3537 1394
Size: 429 1714 3507
Fragments arranged by size:
3507 1714 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2530 2619 4145 4319 65
Size: 1183 1282 89 1526 174 1396
Fragments arranged by size:
1526 1396 1282 1183 174 89
BsrFI r'CCGG_y
Cuts at: 1700 1810 4294 4481 1700
Size: 110 2484 187 2869
Fragments arranged by size:
2869 2484 187 110
BsrGI T'GTAC_A
Cuts at: 96 2576 96
Size: 2480 3170
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5650
BssSI C'ACGA_G
Cuts at: 2700 3710 4722 2700
Size: 1010 1012 3628
Fragments arranged by size:
3628 1012 1010
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5650
BstBI TT'CG_AA
Cuts at: 4454 4454
Size: 5650
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5650
BstXI CCAn_nnnn'nTGG
Cuts at: 2801 2801
Size: 5650
BstYI r'GATC_y
Cuts at: 1972 2283 2980 3347 3732 3749 5201 5287 5299
Size: 311 697 367 385 17 1452 86 12
Cuts at: 5299 5645 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 697 385 367 346 311 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2051 2936 2051
Size: 885 4765
BtgI C'CryG_G
Cuts at: 513 1065 2004 2361 2518 513
Size: 552 939 357 157 3645
Fragments arranged by size:
3645 939 552 357 157
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2780 487
Size: 683 56 1554 3357
Fragments arranged by size:
3357 1554 683 56
BtsI GCAGTG_nn'
Cuts at: 2217 2396 3166 3960 3980 2217
Size: 179 770 794 20 3887
Fragments arranged by size:
3887 794 770 179 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5650
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2236 2271 529
Size: 35 1672 35 3908
Fragments arranged by size:
3908 1672 35 35
DraI TTT'AAA
Cuts at: 2954 2954
Size: 5650
DraIII CAC_nnn'GTG
Cuts at: 2633 2633
Size: 5650
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4657 809
Size: 507 3341 1802
Fragments arranged by size:
3341 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2358 3097 3986
Size: 54 1425 50 275 18 528 739 889
Cuts at: 3986 5615 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 739 528 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3097 1537
Size: 275 1285 4090
Fragments arranged by size:
4090 1285 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2105 2700 3580 1353
Size: 752 595 880 3423
Fragments arranged by size:
3423 880 752 595
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4237 4611 4757 387
Size: 1064 138 2648 374 146 1280
Fragments arranged by size:
2648 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2262 2360 3713 4310 5097 5365
Size: 393 458 172 98 1353 597 787 268
Cuts at: 5365 1239
Size: 1524
Fragments arranged by size:
1524 1353 787 597 458 393 268 172
98
EcoRI G'AATT_C
Cuts at: 2916 2959 3337 2916
Size: 43 378 5229
Fragments arranged by size:
5229 378 43
FspI TGC'GCA
Cuts at: 4156 4156
Size: 5650
HaeII r_GCGC'y
Cuts at: 1678 4797 1678
Size: 3119 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2491 2523 2681 2713 4291
Size: 32 135 32 919 32 158 32 1578
Cuts at: 4291 4323 4365 4397 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1578 919 158 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2994 3240 669
Size: 988 281 1056 246 3079
Fragments arranged by size:
3079 1056 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2464 748
Size: 1716 3934
HpaI GTT'AAC
Cuts at: 3240 3240
Size: 5650
KpnI G_GTAC'C
Cuts at: 2975 2975
Size: 5650
MfeI C'AATT_G
Cuts at: 3249 3249
Size: 5650
MluI A'CGCG_T
Cuts at: 5526 5526
Size: 5650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2250 4764 4948 1305
Size: 945 2514 184 2007
Fragments arranged by size:
2514 2007 945 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2360 10
Size: 54 1425 343 528 3300
Fragments arranged by size:
3300 1425 528 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2523 2556 2745 2834 3608 3967 4126
Size: 648 1357 33 189 89 774 359 159
Cuts at: 4126 518
Size: 2042
Fragments arranged by size:
2042 1357 774 648 359 189 159 89
33
MspA1I CmG'CkG
Cuts at: 1606 2311 3066 3743 4891 5136 1606
Size: 705 755 677 1148 245 2120
Fragments arranged by size:
2120 1148 755 705 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2361 2518 513
Size: 552 939 357 157 3645
Fragments arranged by size:
3645 939 552 357 157
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5650
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5650
NotI GC'GGCC_GC
Cuts at: 3097 3097
Size: 5650
NspI r_CATG'y
Cuts at: 1132 1229 2733 3008 4553 1132
Size: 97 1504 275 1545 2229
Fragments arranged by size:
2229 1545 1504 275 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5650
PciI A'CATG_T
Cuts at: 2729 4549 2729
Size: 1820 3830
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2777 1108
Size: 1669 3981
PfoI T'CCnGG_A
Cuts at: 2062 2337 2062
Size: 275 5375
PmeI GTTT'AAAC
Cuts at: 2954 2954
Size: 5650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3719 3751 5232 5264 3719
Size: 32 1481 32 4105
Fragments arranged by size:
4105 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5650
PsiI TTA'TAA
Cuts at: 3220 3220
Size: 5650
PstI C_TGCA'G
Cuts at: 830 3002 830
Size: 2172 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5650
PvuII CAG'CTG
Cuts at: 2311 2311
Size: 5650
SalI G'TCGA_C
Cuts at: 1936 2992 1936
Size: 1056 4594
SapI GCTCTTCn'nnn_
Cuts at: 2700 2700
Size: 5650
SbfI CC_TGCA'GG
Cuts at: 3002 3002
Size: 5650
ScaI AGT'ACT
Cuts at: 3898 5610 3898
Size: 1712 3938
SfcI C'TryA_G
Cuts at: 826 1045 2884 2998 4133 4814 5005 826
Size: 219 1839 114 1135 681 191 1471
Fragments arranged by size:
1839 1471 1135 681 219 191 114
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5650
SmaI CCC'GGG
Cuts at: 1872 2977 1872
Size: 1105 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3754 4655 4917 820
Size: 197 926 1811 901 262 1553
Fragments arranged by size:
1811 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3441 493
Size: 2948 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5650
SphI G_CATG'C
Cuts at: 1132 3008 1132
Size: 1876 3774
SspI AAT'ATT
Cuts at: 5 52 3574 5
Size: 47 3522 2081
Fragments arranged by size:
3522 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5650
StyI C'CwwG_G
Cuts at: 513 1065 2004 2361 2518 3089 513
Size: 552 939 357 157 571 3074
Fragments arranged by size:
3074 939 571 552 357 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3677 3694 3847 4032 4477 325
Size: 1255 2097 17 153 185 445 1498
Fragments arranged by size:
2097 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2476 2576 3896
Size: 274 80 33 51 1443 499 100 1320
Cuts at: 3896 5608 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1320 499 274 138 100 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2008 2040 5610 5642 2008
Size: 32 3570 32 2016
Fragments arranged by size:
3570 2016 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5650
XbaI T'CTAG_A
Cuts at: 2986 2986
Size: 5650
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2632 2632
Size: 5650
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5650
XmaI C'CCGG_G
Cuts at: 1870 2975 1870
Size: 1105 4545
XmnI GAAnn'nnTTC
Cuts at: 3779 3779
Size: 5650
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3458 276
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI