Restriction map for
pFN21AB7262
(Circular) (Six-base) MAPSORT of: pFN21AB7262.seq Check: 13 from: 1 to: 6058
pFN21AB7262 6058 bp
With 182 enzymes: SgfI *
February 27, 2009 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3868 278
Size: 53 83 186 936 2332 2468
Fragments arranged by size:
2468 2332 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3401 1937
Size: 1464 4594
Acc65I G'GTAC_C
Cuts at: 3379 3379
Size: 6058
AclI AA'CG_TT
Cuts at: 1563 4185 4558 1563
Size: 2622 373 3063
Fragments arranged by size:
3063 2622 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2359 2677 2756 3330 4121 5505 5773 1632
Size: 727 318 79 574 791 1384 268 1917
Fragments arranged by size:
1917 1384 791 727 574 318 268 79
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5861
AflIII A'CryG_T
Cuts at: 1203 2094 4957 5934 1203
Size: 891 2863 977 1327
Fragments arranged by size:
2863 1327 977 891
AgeI A'CCGG_T
Cuts at: 4889 4889
Size: 6058
AhdI GACnn_n'nnGTC
Cuts at: 4787 4787
Size: 6058
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6058
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6058
AlwNI CAG_nnn'CTG
Cuts at: 2966 3302 3801 5373 2966
Size: 336 499 1572 3651
Fragments arranged by size:
3651 1572 499 336
ApaI G_GGCC'C
Cuts at: 2323 2323
Size: 6058
ApaLI G'TGCA_C
Cuts at: 4115 5271 4115
Size: 1156 4902
ApoI r'AATT_y
Cuts at: 1493 3367 3593 3745 5854 1493
Size: 1874 226 152 2109 1697
Fragments arranged by size:
2109 1874 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4612 160
Size: 872 3580 1606
Fragments arranged by size:
3580 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6058
AvaI C'yCGr_G
Cuts at: 1870 1943 2012 2079 2635 2912 3383 1870
Size: 73 69 67 556 277 471 4545
Fragments arranged by size:
4545 556 471 277 73 69 67
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3018 3051 3018
Size: 33 6025
BamHI G'GATC_C
Cuts at: 2599 3388 3755 2599
Size: 789 367 4902
Fragments arranged by size:
4902 789 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2142 2201 2639 2799 3004
Size: 325 217 627 355 59 438 160 205
Cuts at: 3004 3379 4834 618
Size: 375 1455 1842
Fragments arranged by size:
1842 1455 627 438 375 355 325 217
205 160 59
BanII G_rGCy'C
Cuts at: 1391 2086 2323 3377 1391
Size: 695 237 1054 4072
Fragments arranged by size:
4072 1054 695 237
BbeI G_GCGC'C
Cuts at: 2205 2205
Size: 6058
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2780 3278 928
Size: 971 881 498 3708
Fragments arranged by size:
3708 971 881 498
BbvCI CC'TCA_GC
Cuts at: 2660 2660
Size: 6058
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4249 4283 1903
Size: 34 2312 34 3678
Fragments arranged by size:
3678 2312 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3046 3950 5160 3046
Size: 904 1210 3944
Fragments arranged by size:
3944 1210 904
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6058
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4669 136
Size: 107 122 71 631 605 2997 1525
Fragments arranged by size:
2997 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2370 2445 3099 6053 2370
Size: 75 654 2954 2375
Fragments arranged by size:
2954 2375 654 75
BlpI GC'TnA_GC
Cuts at: 3475 3475
Size: 6058
Bme1580I G_kGCm'C
Cuts at: 1790 2323 2383 2642 4119 5275 1790
Size: 533 60 259 1477 1156 2573
Fragments arranged by size:
2573 1477 1156 533 259 60
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6058
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3789 4747 442
Size: 416 53 83 399 24 2372 958 1753
Fragments arranged by size:
2372 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6058
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2610 2610
Size: 6058
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3324 4718 1239
Size: 2085 1394 2579
Fragments arranged by size:
2579 2085 1394
Bpu10I CC'TnA_GC
Cuts at: 2660 2660
Size: 6058
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4183 5048 5346 4183
Size: 865 298 4895
Fragments arranged by size:
4895 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4721 882
Size: 617 3222 2219
Fragments arranged by size:
3222 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3849 493
Size: 711 2645 2702
Fragments arranged by size:
2702 2645 711
BsaBI GATnn'nnATC
Cuts at: 6052 6052
Size: 6058
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2202 3865 4247 4882
Size: 53 83 186 936 669 1663 382 635
Cuts at: 4882 275
Size: 1451
Fragments arranged by size:
1663 1451 936 669 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4491 4889 5163 5310 5721 4491
Size: 398 274 147 411 4828
Fragments arranged by size:
4828 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5833
Fragments arranged by size:
5833 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2020 1186
Size: 834 5224
BseYI C'CCAG_C
Cuts at: 2038 2250 2485 2618 2644 2924 5261 5757 2038
Size: 212 235 133 26 280 2337 496 2339
Fragments arranged by size:
2339 2337 496 280 235 212 133 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2416 2588 1163
Size: 573 680 172 4633
Fragments arranged by size:
4633 680 573 172
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3508 4269 5297 1540
Size: 275 186 1507 761 1028 2301
Fragments arranged by size:
2301 1507 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4119 4204 5275 4119
Size: 85 1071 4902
Fragments arranged by size:
4902 1071 85
BsmI GAATG_Cn'
Cuts at: 3569 3662 3569
Size: 93 5965
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2086 2323 2383 2642 3377 4119 4204
Size: 399 296 237 60 259 735 742 85
Cuts at: 4204 5275 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 735 399 296 259 237
85 60
BspEI T'CCGG_A
Cuts at: 5721 5721
Size: 6058
BspHI T'CATG_A
Cuts at: 3947 5677 3947
Size: 1730 4328
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2198 2474 3063 3413 844
Size: 1354 276 589 350 3489
Fragments arranged by size:
3489 1354 589 350 276
BsrBI CCG'CTC
Cuts at: 1394 1823 3945 1394
Size: 429 2122 3507
Fragments arranged by size:
3507 2122 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2279 4553 4727 65
Size: 1183 1031 2274 174 1396
Fragments arranged by size:
2274 1396 1183 1031 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4702 4889 1700
Size: 110 2892 187 2869
Fragments arranged by size:
2892 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6058
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6058
BssSI C'ACGA_G
Cuts at: 3077 4118 5130 3077
Size: 1041 1012 4005
Fragments arranged by size:
4005 1041 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2841 2943 794
Size: 2047 102 3909
Fragments arranged by size:
3909 2047 102
BstBI TT'CG_AA
Cuts at: 4862 4862
Size: 6058
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6058
BstXI CCAn_nnnn'nTGG
Cuts at: 2290 2290
Size: 6058
BstYI r'GATC_y
Cuts at: 1972 2370 2445 2599 3099 3388 3755 4140 4157
Size: 398 75 154 500 289 367 385 17
Cuts at: 4157 5609 5695 5707 6053 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 500 398 385 367 346 289
154 86 75 17 12
Bsu36I CC'TnA_GG
Cuts at: 2595 2595
Size: 6058
BtgI C'CryG_G
Cuts at: 513 1065 2004 2110 2578 2785 513
Size: 552 939 106 468 207 3786
Fragments arranged by size:
3786 939 552 468 207 106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5319
Fragments arranged by size:
5319 683 56
BtsI GCAGTG_nn'
Cuts at: 2242 3574 4368 4388 2242
Size: 1332 794 20 3912
Fragments arranged by size:
3912 1332 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6058
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6023
DraI TTT'AAA
Cuts at: 3362 3362
Size: 6058
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5065 809
Size: 507 3749 1802
Fragments arranged by size:
3749 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2857 2968 3319
Size: 54 1425 50 275 18 1027 111 351
Cuts at: 3319 3505 4394 6023 8
Size: 186 889 1629 43
Fragments arranged by size:
1629 1425 1027 889 351 275 186 111
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3505 1537
Size: 275 1693 4090
Fragments arranged by size:
4090 1693 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2111 2550 3988 1353
Size: 758 439 1438 3423
Fragments arranged by size:
3423 1438 758 439
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2223 4645 5019 5165 387
Size: 1064 138 634 2422 374 146 1280
Fragments arranged by size:
2422 1280 1064 634 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2359 2677 2756 3324 3330 4121 4718
Size: 393 727 318 79 568 6 791 597
Cuts at: 4718 5505 5773 1239
Size: 787 268 1524
Fragments arranged by size:
1524 791 787 727 597 568 393 318
268 79 6
EcoO109I rG'GnC_Cy
Cuts at: 2023 2320 3016 2023
Size: 297 696 5065
Fragments arranged by size:
5065 696 297
EcoRI G'AATT_C
Cuts at: 3367 3745 3367
Size: 378 5680
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3239 3239
Size: 6058
FspI TGC'GCA
Cuts at: 4564 4564
Size: 6058
HaeII r_GCGC'y
Cuts at: 1678 2205 2667 5205 1678
Size: 527 462 2538 2531
Fragments arranged by size:
2538 2531 527 462
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4699 4731 4773 4805 1373
Size: 32 135 32 3127 32 42 32 2626
Fragments arranged by size:
3127 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3402 3648 669
Size: 988 281 1464 246 3079
Fragments arranged by size:
3079 1464 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6058
HpaI GTT'AAC
Cuts at: 3648 3648
Size: 6058
KasI G'GCGC_C
Cuts at: 2201 2201
Size: 6058
KpnI G_GTAC'C
Cuts at: 3383 3383
Size: 6058
MfeI C'AATT_G
Cuts at: 3657 3657
Size: 6058
MluI A'CGCG_T
Cuts at: 5934 5934
Size: 6058
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3272 5172 5356 1305
Size: 1967 1900 184 2007
Fragments arranged by size:
2007 1967 1900 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2970 10
Size: 54 1425 343 1138 3098
Fragments arranged by size:
3098 1425 1138 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2272 4016 4375 4534 518
Size: 648 1106 1744 359 159 2042
Fragments arranged by size:
2042 1744 1106 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2430 2500 3474 4151 5299 5544 1606
Size: 824 70 974 677 1148 245 2120
Fragments arranged by size:
2120 1148 974 824 677 245 70
NarI GG'CG_CC
Cuts at: 2202 2202
Size: 6058
NcoI C'CATG_G
Cuts at: 513 1065 2004 2110 2578 513
Size: 552 939 106 468 3993
Fragments arranged by size:
3993 939 552 468 106
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6058
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6058
NotI GC'GGCC_GC
Cuts at: 3505 3505
Size: 6058
NspI r_CATG'y
Cuts at: 1132 1229 2098 3416 4961 1132
Size: 97 869 1318 1545 2229
Fragments arranged by size:
2229 1545 1318 869 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6058
PciI A'CATG_T
Cuts at: 2094 4957 2094
Size: 2863 3195
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3302 1108
Size: 2194 3864
PmeI GTTT'AAAC
Cuts at: 3362 3362
Size: 6058
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4127 4159 5640 5672 4127
Size: 32 1481 32 4513
Fragments arranged by size:
4513 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2023 3016 2023
Size: 993 5065
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6058
PsiI TTA'TAA
Cuts at: 3628 3628
Size: 6058
PspOMI G'GGCC_C
Cuts at: 2319 2319
Size: 6058
PspXI vC'TCGA_Gb
Cuts at: 2079 2079
Size: 6058
PstI C_TGCA'G
Cuts at: 830 2429 3410 830
Size: 1599 981 3478
Fragments arranged by size:
3478 1599 981
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6058
PvuII CAG'CTG
Cuts at: 2430 2430
Size: 6058
SalI G'TCGA_C
Cuts at: 1936 3400 1936
Size: 1464 4594
SbfI CC_TGCA'GG
Cuts at: 3410 3410
Size: 6058
ScaI AGT'ACT
Cuts at: 4306 6018 4306
Size: 1712 4346
SexAI A'CCwGG_T
Cuts at: 2990 2990
Size: 6058
SfcI C'TryA_G
Cuts at: 826 1045 2425 3406 4541 5222 5413 826
Size: 219 1380 981 1135 681 191 1471
Fragments arranged by size:
1471 1380 1135 981 681 219 191
SfoI GGC'GCC
Cuts at: 2203 2203
Size: 6058
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6058
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6058
SmaI CCC'GGG
Cuts at: 1872 2637 3385 1872
Size: 765 748 4545
Fragments arranged by size:
4545 765 748
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2079 4162 5063 5325 820
Size: 197 926 136 2083 901 262 1553
Fragments arranged by size:
2083 1553 926 901 262 197 136
SnaBI TAC'GTA
Cuts at: 493 3849 493
Size: 3356 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6058
SphI G_CATG'C
Cuts at: 1132 3416 1132
Size: 2284 3774
SrfI GCCC'GGGC
Cuts at: 2637 2637
Size: 6058
SspI AAT'ATT
Cuts at: 5 52 3982 5
Size: 47 3930 2081
Fragments arranged by size:
3930 2081 47
StuI AGG'CCT
Cuts at: 1517 2166 2522 3083 1517
Size: 649 356 561 4492
Fragments arranged by size:
4492 649 561 356
StyI C'CwwG_G
Cuts at: 513 1065 2004 2110 2578 2700 3000 3164 3497
Size: 552 939 106 468 122 300 164 333
Cuts at: 3497 513
Size: 3074
Fragments arranged by size:
3074 939 552 468 333 300 164 122
106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4085 4102 4255 4440 4885 325
Size: 1255 2505 17 153 185 445 1498
Fragments arranged by size:
2505 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2172 4304 6016
Size: 274 80 33 51 1443 195 2132 1712
Cuts at: 6016 96
Size: 138
Fragments arranged by size:
2132 1712 1443 274 195 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3229 3261 3308 3340 6018 6050 3229
Size: 32 47 32 2678 32 3237
Fragments arranged by size:
3237 2678 47 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6058
XbaI T'CTAG_A
Cuts at: 3394 3394
Size: 6058
XhoI C'TCGA_G
Cuts at: 1943 2079 1943
Size: 136 5922
XmaI C'CCGG_G
Cuts at: 1870 2635 3383 1870
Size: 765 748 4545
Fragments arranged by size:
4545 765 748
XmnI GAAnn'nnTTC
Cuts at: 3285 4187 3285
Size: 902 5156
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3866 276
Size: 53 83 186 936 2332 2468
Fragments arranged by size:
2468 2332 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BfrBI BsiWI BsmBI
BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PmlI PsrI RsrII
SacI SacII SanDI SapI SfiI SwaI XcmI