Restriction map for
pFN21AB7256
(Circular) (Six-base) MAPSORT of: pFN21AB7256.seq Check: 417 from: 1 to: 6028
pFN21AB7256 6028 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3838 278
Size: 53 83 186 936 2302 2468
Fragments arranged by size:
2468 2302 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2181 2553 3371 1937
Size: 244 372 818 4594
Fragments arranged by size:
4594 818 372 244
Acc65I G'GTAC_C
Cuts at: 2253 3349 2253
Size: 1096 4932
AclI AA'CG_TT
Cuts at: 1563 4155 4528 1563
Size: 2592 373 3063
Fragments arranged by size:
3063 2592 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2839 3158 4091 5475 5743 1632
Size: 1207 319 933 1384 268 1917
Fragments arranged by size:
1917 1384 1207 933 319 268
AfeI AGC'GCT
Cuts at: 2671 2671
Size: 6028
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5831
AflIII A'CryG_T
Cuts at: 1203 2865 4927 5904 1203
Size: 1662 2062 977 1327
Fragments arranged by size:
2062 1662 1327 977
AgeI A'CCGG_T
Cuts at: 4859 4859
Size: 6028
AhdI GACnn_n'nnGTC
Cuts at: 4757 4757
Size: 6028
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6028
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6028
AlwNI CAG_nnn'CTG
Cuts at: 3771 5343 3771
Size: 1572 4456
ApaI G_GGCC'C
Cuts at: 2350 3076 2350
Size: 726 5302
ApaLI G'TGCA_C
Cuts at: 4085 5241 4085
Size: 1156 4872
ApoI r'AATT_y
Cuts at: 1493 2058 2276 2978 3337 3563 3715 5824 1493
Size: 565 218 702 359 226 152 2109 1697
Fragments arranged by size:
2109 1697 702 565 359 226 218 152
AseI AT'TA_AT
Cuts at: 160 1032 4582 160
Size: 872 3550 1606
Fragments arranged by size:
3550 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6028
AvaI C'yCGr_G
Cuts at: 1870 1943 3353 1870
Size: 73 1410 4545
Fragments arranged by size:
4545 1410 73
BamHI G'GATC_C
Cuts at: 3358 3725 3358
Size: 367 5661
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2253 2715 2878 3349 4804
Size: 325 217 627 466 462 163 471 1455
Cuts at: 4804 618
Size: 1842
Fragments arranged by size:
1842 1455 627 471 466 462 325 217
163
BanII G_rGCy'C
Cuts at: 1391 2350 2576 3076 3347 1391
Size: 959 226 500 271 4072
Fragments arranged by size:
4072 959 500 271 226
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2553 2586 2845 928
Size: 971 654 33 259 4111
Fragments arranged by size:
4111 971 654 259 33
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4219 4253 1903
Size: 34 2282 34 3678
Fragments arranged by size:
3678 2282 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3920 5130 3920
Size: 1210 4818
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6028
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2345 4639 136
Size: 107 122 71 631 605 673 2294 1525
Fragments arranged by size:
2294 1525 673 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2319 6023 2319
Size: 3704 2324
BlpI GC'TnA_GC
Cuts at: 2953 3445 2953
Size: 492 5536
Bme1580I G_kGCm'C
Cuts at: 1790 2350 2916 3076 3313 4089 5245 1790
Size: 560 566 160 237 776 1156 2573
Fragments arranged by size:
2573 1156 776 566 560 237 160
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6028
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3759 4717 442
Size: 416 53 83 399 24 2342 958 1753
Fragments arranged by size:
2342 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6028
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2919 2919
Size: 6028
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2278 2694 4688 1239
Size: 1039 416 1994 2579
Fragments arranged by size:
2579 1994 1039 416
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4153 5018 5316 4153
Size: 865 298 4865
Fragments arranged by size:
4865 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2140 2464 4691 882
Size: 617 641 324 2227 2219
Fragments arranged by size:
2227 2219 641 617 324
BsaAI yAC'GTr
Cuts at: 493 1204 3819 493
Size: 711 2615 2702
Fragments arranged by size:
2702 2615 711
BsaBI GATnn'nnATC
Cuts at: 2076 6022 2076
Size: 3946 2082
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3835 4217 4852 275
Size: 53 83 186 936 2302 382 635 1451
Fragments arranged by size:
2302 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4461 4859 5133 5280 5691 4461
Size: 398 274 147 411 4798
Fragments arranged by size:
4798 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3200 3230 1178
Size: 30 165 30 1797 30 3976
Fragments arranged by size:
3976 1797 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2584 1186
Size: 1398 4630
BseYI C'CCAG_C
Cuts at: 3123 3274 5231 5727 3123
Size: 151 1957 496 3424
Fragments arranged by size:
3424 1957 496 151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2488 1163
Size: 573 752 4703
Fragments arranged by size:
4703 752 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3478 4239 5267 1540
Size: 275 186 1477 761 1028 2301
Fragments arranged by size:
2301 1477 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2900 3117 3302 4089 4174 5245 2900
Size: 217 185 787 85 1071 3683
Fragments arranged by size:
3683 1071 787 217 185 85
BsmI GAATG_Cn'
Cuts at: 2529 3539 3632 2529
Size: 1010 93 4925
Fragments arranged by size:
4925 1010 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2350 2576 2900 2916 3076 3117 3302
Size: 399 560 226 324 16 160 41 185
Cuts at: 3302 3313 3347 4089 4174 5245 1391
Size: 11 34 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 560 399 324 226 185
160 85 41 34 16 11
BspEI T'CCGG_A
Cuts at: 5691 5691
Size: 6028
BspHI T'CATG_A
Cuts at: 3917 5647 3917
Size: 1730 4298
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2799 3383 844
Size: 1955 584 3489
Fragments arranged by size:
3489 1955 584
BsrBI CCG'CTC
Cuts at: 1394 1823 3915 1394
Size: 429 2092 3507
Fragments arranged by size:
3507 2092 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4523 4697 65
Size: 1183 3275 174 1396
Fragments arranged by size:
3275 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4672 4859 1700
Size: 110 2862 187 2869
Fragments arranged by size:
2869 2862 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6028
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6028
BssSI C'ACGA_G
Cuts at: 4088 5100 4088
Size: 1012 5016
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6028
BstBI TT'CG_AA
Cuts at: 4832 4832
Size: 6028
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6028
BstXI CCAn_nnnn'nTGG
Cuts at: 2455 2836 2932 2971 2455
Size: 381 96 39 5512
Fragments arranged by size:
5512 381 96 39
BstYI r'GATC_y
Cuts at: 1972 2319 3279 3358 3725 4110 4127 5579 5665
Size: 347 960 79 367 385 17 1452 86
Cuts at: 5665 5677 6023 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 960 385 367 347 346 86
79 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2882 513
Size: 552 1817 3659
Fragments arranged by size:
3659 1817 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5289
Fragments arranged by size:
5289 683 56
BtsI GCAGTG_nn'
Cuts at: 3544 4338 4358 3544
Size: 794 20 5214
Fragments arranged by size:
5214 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6028
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5993
DraI TTT'AAA
Cuts at: 3332 3332
Size: 6028
DraIII CAC_nnn'GTG
Cuts at: 2888 3297 2888
Size: 409 5619
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2195 5035 809
Size: 507 879 2840 1802
Fragments arranged by size:
2840 1802 879 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3475 4364 5993
Size: 54 1425 50 275 18 1645 889 1629
Cuts at: 5993 8
Size: 43
Fragments arranged by size:
1645 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3475 1537
Size: 275 1663 4090
Fragments arranged by size:
4090 1663 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3257 3958 1353
Size: 1904 701 3423
Fragments arranged by size:
3423 1904 701
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4615 4989 5135 387
Size: 1064 138 3026 374 146 1280
Fragments arranged by size:
3026 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2278 2694 2839 3158 4091 4688 5475
Size: 393 646 416 145 319 933 597 787
Cuts at: 5475 5743 1239
Size: 268 1524
Fragments arranged by size:
1524 933 787 646 597 416 393 319
268 145
EcoNI CCTnn'n_nnAGG
Cuts at: 2566 2566
Size: 6028
EcoO109I rG'GnC_Cy
Cuts at: 2347 3072 2347
Size: 725 5303
EcoRI G'AATT_C
Cuts at: 2058 3337 3715 2058
Size: 1279 378 4371
Fragments arranged by size:
4371 1279 378
EcoRV GAT'ATC
Cuts at: 2356 2356
Size: 6028
FspI TGC'GCA
Cuts at: 2711 4534 2711
Size: 1823 4205
HaeII r_GCGC'y
Cuts at: 1678 2673 5175 1678
Size: 995 2502 2531
Fragments arranged by size:
2531 2502 995
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4669 4701 4743 4775 1373
Size: 32 135 32 3097 32 42 32 2626
Fragments arranged by size:
3097 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2863 3372 3618 669
Size: 988 281 925 509 246 3079
Fragments arranged by size:
3079 988 925 509 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6028
HpaI GTT'AAC
Cuts at: 3618 3618
Size: 6028
KpnI G_GTAC'C
Cuts at: 2257 3353 2257
Size: 1096 4932
MfeI C'AATT_G
Cuts at: 3627 3627
Size: 6028
MluI A'CGCG_T
Cuts at: 5904 5904
Size: 6028
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2895 5142 5326 1305
Size: 1590 2247 184 2007
Fragments arranged by size:
2247 2007 1590 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4206
Fragments arranged by size:
4206 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2045 2453 2768 2930 3986 4345 4504
Size: 648 879 408 315 162 1056 359 159
Cuts at: 4504 518
Size: 2042
Fragments arranged by size:
2042 1056 879 648 408 359 315 162
159
MspA1I CmG'CkG
Cuts at: 1606 3444 4121 5269 5514 1606
Size: 1838 677 1148 245 2120
Fragments arranged by size:
2120 1838 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5476
NdeI CA'TA_TG
Cuts at: 387 2482 387
Size: 2095 3933
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6028
NotI GC'GGCC_GC
Cuts at: 3475 3475
Size: 6028
NspI r_CATG'y
Cuts at: 1132 1229 2302 2869 3386 4931 1132
Size: 97 1073 567 517 1545 2229
Fragments arranged by size:
2229 1545 1073 567 517 97
PasI CC'CwG_GG
Cuts at: 1348 3069 1348
Size: 1721 4307
PciI A'CATG_T
Cuts at: 2865 4927 2865
Size: 2062 3966
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6028
PfoI T'CCnGG_A
Cuts at: 2285 2285
Size: 6028
PmeI GTTT'AAAC
Cuts at: 3332 3332
Size: 6028
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4097 4129 5610 5642 4097
Size: 32 1481 32 4483
Fragments arranged by size:
4483 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6028
PsiI TTA'TAA
Cuts at: 3598 3598
Size: 6028
PspOMI G'GGCC_C
Cuts at: 2346 3072 2346
Size: 726 5302
PstI C_TGCA'G
Cuts at: 830 2056 2664 3380 830
Size: 1226 608 716 3478
Fragments arranged by size:
3478 1226 716 608
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6028
SalI G'TCGA_C
Cuts at: 1936 3370 1936
Size: 1434 4594
SapI GCTCTTCn'nnn_
Cuts at: 3257 3257
Size: 6028
SbfI CC_TGCA'GG
Cuts at: 3380 3380
Size: 6028
ScaI AGT'ACT
Cuts at: 4276 5988 4276
Size: 1712 4316
SexAI A'CCwGG_T
Cuts at: 2874 2874
Size: 6028
SfcI C'TryA_G
Cuts at: 826 1045 2052 2660 3376 4511 5192 5383 826
Size: 219 1007 608 716 1135 681 191 1471
Fragments arranged by size:
1471 1135 1007 716 681 608 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6028
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6028
SmaI CCC'GGG
Cuts at: 1872 3355 1872
Size: 1483 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4132 5033 5295 820
Size: 197 926 2189 901 262 1553
Fragments arranged by size:
2189 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3819 493
Size: 3326 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6028
SphI G_CATG'C
Cuts at: 1132 3386 1132
Size: 2254 3774
SspI AAT'ATT
Cuts at: 5 52 2235 3952 5
Size: 47 2183 1717 2081
Fragments arranged by size:
2183 2081 1717 47
StuI AGG'CCT
Cuts at: 1517 3055 1517
Size: 1538 4490
StyI C'CwwG_G
Cuts at: 513 1065 2166 3467 513
Size: 552 1101 1301 3074
Fragments arranged by size:
3074 1301 1101 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2812 3258 4055 4072 4225 4410 4855
Size: 1255 1232 446 797 17 153 185 445
Cuts at: 4855 325
Size: 1498
Fragments arranged by size:
1498 1255 1232 797 446 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4274 5986 96
Size: 274 80 33 51 1443 2297 1712 138
Fragments arranged by size:
2297 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3200 3232 5988 6020 3200
Size: 32 2756 32 3208
Fragments arranged by size:
3208 2756 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6028
XbaI T'CTAG_A
Cuts at: 3364 3364
Size: 6028
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2182 2836 2182
Size: 654 5374
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6028
XmaI C'CCGG_G
Cuts at: 1870 3353 1870
Size: 1483 4545
XmnI GAAnn'nnTTC
Cuts at: 2583 4157 2583
Size: 1574 4454
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3836 276
Size: 53 83 186 936 2302 2468
Fragments arranged by size:
2468 2302 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SfiI SfoI SrfI SwaI