Restriction map for
pFN21AB7248
(Circular) (Six-base) MAPSORT of: pFN21AB7248.seq Check: 3598 from: 1 to: 6307
pFN21AB7248 6307 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3529 3529
Size: 6307
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4117 278
Size: 53 83 186 936 2581 2468
Fragments arranged by size:
2581 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3650 1937
Size: 1713 4594
Acc65I G'GTAC_C
Cuts at: 3628 3628
Size: 6307
AclI AA'CG_TT
Cuts at: 1563 4434 4807 1563
Size: 2871 373 3063
Fragments arranged by size:
3063 2871 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2321 4370 5754 6022 1632
Size: 689 2049 1384 268 1917
Fragments arranged by size:
2049 1917 1384 689 268
AfeI AGC'GCT
Cuts at: 2766 2766
Size: 6307
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6110
AflIII A'CryG_T
Cuts at: 1203 2931 3162 5206 6183 1203
Size: 1728 231 2044 977 1327
Fragments arranged by size:
2044 1728 1327 977 231
AgeI A'CCGG_T
Cuts at: 5138 5138
Size: 6307
AhdI GACnn_n'nnGTC
Cuts at: 5036 5036
Size: 6307
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6307
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6307
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2804 2836 2804
Size: 32 6275
AlwNI CAG_nnn'CTG
Cuts at: 2371 2649 3575 4050 5622 2371
Size: 278 926 475 1572 3056
Fragments arranged by size:
3056 1572 926 475 278
ApaLI G'TGCA_C
Cuts at: 2102 4364 5520 2102
Size: 2262 1156 2889
Fragments arranged by size:
2889 2262 1156
ApoI r'AATT_y
Cuts at: 1493 3002 3616 3842 3994 6103 1493
Size: 1509 614 226 152 2109 1697
Fragments arranged by size:
2109 1697 1509 614 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4861 160
Size: 872 3829 1606
Fragments arranged by size:
3829 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6307
AvaI C'yCGr_G
Cuts at: 1870 1943 2028 2083 2255 3026 3632 1870
Size: 73 85 55 172 771 606 4545
Fragments arranged by size:
4545 771 606 172 85 73 55
BamHI G'GATC_C
Cuts at: 3637 4004 3637
Size: 367 5940
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2077 3628 5083 618
Size: 325 217 627 290 1551 1455 1842
Fragments arranged by size:
1842 1551 1455 627 325 290 217
BanII G_rGCy'C
Cuts at: 1391 2240 3366 3626 1391
Size: 849 1126 260 4072
Fragments arranged by size:
4072 1126 849 260
BbeI G_GCGC'C
Cuts at: 2081 2081
Size: 6307
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3165 928
Size: 971 1266 4070
Fragments arranged by size:
4070 1266 971
BbvCI CC'TCA_GC
Cuts at: 2117 2117
Size: 6307
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3488 3522 4498 4532 1903
Size: 34 1551 34 976 34 3678
Fragments arranged by size:
3678 1551 976 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4199 5409 4199
Size: 1210 5097
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6307
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2086 2141 4918
Size: 107 122 71 631 605 414 55 2777
Cuts at: 4918 136
Size: 1525
Fragments arranged by size:
2777 1525 631 605 414 122 107 71
55
BglII A'GATC_T
Cuts at: 3055 6302 3055
Size: 3247 3060
BlpI GC'TnA_GC
Cuts at: 3724 3724
Size: 6307
Bme1580I G_kGCm'C
Cuts at: 1790 2106 4368 5524 1790
Size: 316 2262 1156 2573
Fragments arranged by size:
2573 2262 1156 316
BmgBI CAC'GTC
Cuts at: 1318 2748 1318
Size: 1430 4877
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4038 4996 442
Size: 416 53 83 399 24 2621 958 1753
Fragments arranged by size:
2621 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6307
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2029 2706 2781 4967 1239
Size: 790 677 75 2186 2579
Fragments arranged by size:
2579 2186 790 677 75
Bpu10I CC'TnA_GC
Cuts at: 2117 2117
Size: 6307
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3323 4432 5297 5595 3323
Size: 1109 865 298 4035
Fragments arranged by size:
4035 1109 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3506 4970 882
Size: 617 2007 1464 2219
Fragments arranged by size:
2219 2007 1464 617
BsaAI yAC'GTr
Cuts at: 493 1204 4098 493
Size: 711 2894 2702
Fragments arranged by size:
2894 2702 711
BsaBI GATnn'nnATC
Cuts at: 6301 6301
Size: 6307
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2078 2424 4114 4496
Size: 53 83 186 936 545 346 1690 382
Cuts at: 4496 5131 275
Size: 635 1451
Fragments arranged by size:
1690 1451 936 635 545 382 346 186
83 53
BsaWI w'CCGG_w
Cuts at: 4740 5138 5412 5559 5970 4740
Size: 398 274 147 411 5077
Fragments arranged by size:
5077 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2679 2709 1178
Size: 30 165 30 1276 30 4776
Fragments arranged by size:
4776 1276 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2861 2864 3108 3416 1186
Size: 1675 3 244 308 4077
Fragments arranged by size:
4077 1675 308 244 3
BseYI C'CCAG_C
Cuts at: 3575 5510 6006 3575
Size: 1935 496 3876
Fragments arranged by size:
3876 1935 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3528 1163
Size: 573 1792 3942
Fragments arranged by size:
3942 1792 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3245 3757 4518 5546 1540
Size: 275 186 1244 512 761 1028 2301
Fragments arranged by size:
2301 1244 1028 761 512 275 186
BsiHKAI G_wGCw'C
Cuts at: 2106 3366 4368 4453 5524 2106
Size: 1260 1002 85 1071 2889
Fragments arranged by size:
2889 1260 1071 1002 85
BsmI GAATG_Cn'
Cuts at: 2675 3818 3911 2675
Size: 1143 93 5071
Fragments arranged by size:
5071 1143 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2572 2981 2572
Size: 409 5898
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2106 2240 3366 3626 4368 4453 5524
Size: 399 316 134 1126 260 742 85 1071
Cuts at: 5524 1391
Size: 2174
Fragments arranged by size:
2174 1126 1071 742 399 316 260 134
85
BspEI T'CCGG_A
Cuts at: 5970 5970
Size: 6307
BspHI T'CATG_A
Cuts at: 2148 4196 5926 2148
Size: 2048 1730 2529
Fragments arranged by size:
2529 2048 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2657 3213 3529 3662 844
Size: 1813 556 316 133 3489
Fragments arranged by size:
3489 1813 556 316 133
BsrBI CCG'CTC
Cuts at: 1394 1823 3186 4194 1394
Size: 429 1363 1008 3507
Fragments arranged by size:
3507 1363 1008 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4802 4976 65
Size: 1183 3554 174 1396
Fragments arranged by size:
3554 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2330 3240 3244 4951 5138 1700
Size: 110 520 910 4 1707 187 2869
Fragments arranged by size:
2869 1707 910 520 187 110 4
BsrGI T'GTAC_A
Cuts at: 96 2636 2832 96
Size: 2540 196 3571
Fragments arranged by size:
3571 2540 196
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6307
BssSI C'ACGA_G
Cuts at: 2105 3014 4367 5379 2105
Size: 909 1353 1012 3033
Fragments arranged by size:
3033 1353 1012 909
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2110 2371 794
Size: 1316 261 4730
Fragments arranged by size:
4730 1316 261
BstBI TT'CG_AA
Cuts at: 3498 5111 3498
Size: 1613 4694
BstEII G'GTnAC_C
Cuts at: 1184 3320 1184
Size: 2136 4171
BstXI CCAn_nnnn'nTGG
Cuts at: 3271 3271
Size: 6307
BstYI r'GATC_y
Cuts at: 1972 3055 3637 4004 4389 4406 5858 5944 5956
Size: 1083 582 367 385 17 1452 86 12
Cuts at: 5956 6302 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1083 582 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5755
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5568
Fragments arranged by size:
5568 683 56
BtsI GCAGTG_nn'
Cuts at: 3823 4617 4637 3823
Size: 794 20 5493
Fragments arranged by size:
5493 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6307
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6272
DraI TTT'AAA
Cuts at: 3611 3611
Size: 6307
DraIII CAC_nnn'GTG
Cuts at: 2322 2347 2790 2322
Size: 25 443 5839
Fragments arranged by size:
5839 443 25
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2973 3308 5314 809
Size: 507 1657 335 2006 1802
Fragments arranged by size:
2006 1802 1657 507 335
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2349 2385 2603
Size: 54 1425 50 275 18 519 36 218
Cuts at: 2603 2898 3242 3754 4643 6272 8
Size: 295 344 512 889 1629 43
Fragments arranged by size:
1629 1425 889 519 512 344 295 275
218 54 50 43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 3242 3754 1537
Size: 275 1430 512 4090
Fragments arranged by size:
4090 1430 512 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2168 2849 4237 1353
Size: 815 681 1388 3423
Fragments arranged by size:
3423 1388 815 681
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2104 2975 3595 4894 5268 5414
Size: 1064 138 515 871 620 1299 374 146
Cuts at: 5414 387
Size: 1280
Fragments arranged by size:
1299 1280 1064 871 620 515 374 146
138
EcoICRI GAG'CTC
Cuts at: 3364 3364
Size: 6307
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2029 2321 2706 2781 4370 4967 5754
Size: 393 397 292 385 75 1589 597 787
Cuts at: 5754 6022 1239
Size: 268 1524
Fragments arranged by size:
1589 1524 787 597 397 393 385 292
268 75
EcoNI CCTnn'n_nnAGG
Cuts at: 2129 2300 2129
Size: 171 6136
EcoO109I rG'GnC_Cy
Cuts at: 2646 2756 3022 2646
Size: 110 266 5931
Fragments arranged by size:
5931 266 110
EcoRI G'AATT_C
Cuts at: 3616 3994 3616
Size: 378 5929
EcoRV GAT'ATC
Cuts at: 2717 2717
Size: 6307
FseI GG_CCGG'CC
Cuts at: 3248 3248
Size: 6307
FspI TGC'GCA
Cuts at: 2315 4813 2315
Size: 2498 3809
FspAI rTGC'GCAy
Cuts at: 2315 2315
Size: 6307
HaeII r_GCGC'y
Cuts at: 1678 2081 2124 2372 2693 2768 5454 1678
Size: 403 43 248 321 75 2686 2531
Fragments arranged by size:
2686 2531 403 321 248 75 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2679 2711 3053 3085 4948
Size: 32 135 32 1107 32 342 32 1863
Cuts at: 4948 4980 5022 5054 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1863 1107 342 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3651 3897 669
Size: 988 281 1713 246 3079
Fragments arranged by size:
3079 1713 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3044 748
Size: 2296 4011
HpaI GTT'AAC
Cuts at: 3897 3897
Size: 6307
KasI G'GCGC_C
Cuts at: 2077 2077
Size: 6307
KpnI G_GTAC'C
Cuts at: 3632 3632
Size: 6307
MfeI C'AATT_G
Cuts at: 3906 3906
Size: 6307
MluI A'CGCG_T
Cuts at: 6183 6183
Size: 6307
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2616 5421 5605 1305
Size: 1311 2805 184 2007
Fragments arranged by size:
2805 2007 1311 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2605 10
Size: 54 1425 343 773 3712
Fragments arranged by size:
3712 1425 773 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2432 2444 2459 2746 4265 4624 4783
Size: 648 1266 12 15 287 1519 359 159
Cuts at: 4783 518
Size: 2042
Fragments arranged by size:
2042 1519 1266 648 359 287 159 15
12
MspA1I CmG'CkG
Cuts at: 1606 2189 2950 2984 3192 3271 3723 4400 5548
Size: 583 761 34 208 79 452 677 1148
Cuts at: 5548 5793 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 761 677 583 452 245 208
79 34
NaeI GCC'GGC
Cuts at: 2332 3242 3246 2332
Size: 910 4 5393
Fragments arranged by size:
5393 910 4
NarI GG'CG_CC
Cuts at: 2078 2078
Size: 6307
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5755
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6307
NgoMIV G'CCGG_C
Cuts at: 2330 3240 3244 2330
Size: 910 4 5393
Fragments arranged by size:
5393 910 4
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6307
NotI GC'GGCC_GC
Cuts at: 3754 3754
Size: 6307
NspI r_CATG'y
Cuts at: 1132 1229 3166 3665 5210 1132
Size: 97 1937 499 1545 2229
Fragments arranged by size:
2229 1937 1545 499 97
PasI CC'CwG_GG
Cuts at: 1348 2233 3349 1348
Size: 885 1116 4306
Fragments arranged by size:
4306 1116 885
PciI A'CATG_T
Cuts at: 3162 5206 3162
Size: 2044 4263
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2347 2704 2742 1108
Size: 1239 357 38 4673
Fragments arranged by size:
4673 1239 357 38
PmeI GTTT'AAAC
Cuts at: 3611 3611
Size: 6307
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4376 4408 5889 5921 4376
Size: 32 1481 32 4762
Fragments arranged by size:
4762 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2646 2756 2646
Size: 110 6197
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6307
PsiI TTA'TAA
Cuts at: 3877 3877
Size: 6307
PstI C_TGCA'G
Cuts at: 830 2130 3659 830
Size: 1300 1529 3478
Fragments arranged by size:
3478 1529 1300
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6307
PvuII CAG'CTG
Cuts at: 2950 3271 2950
Size: 321 5986
RsrII CG'GwC_CG
Cuts at: 2170 2170
Size: 6307
SacI G_AGCT'C
Cuts at: 3366 3366
Size: 6307
SalI G'TCGA_C
Cuts at: 1936 3649 1936
Size: 1713 4594
SapI GCTCTTCn'nnn_
Cuts at: 2168 2168
Size: 6307
SbfI CC_TGCA'GG
Cuts at: 3659 3659
Size: 6307
ScaI AGT'ACT
Cuts at: 4555 6267 4555
Size: 1712 4595
SexAI A'CCwGG_T
Cuts at: 2668 2668
Size: 6307
SfcI C'TryA_G
Cuts at: 826 1045 2126 3655 4790 5471 5662 826
Size: 219 1081 1529 1135 681 191 1471
Fragments arranged by size:
1529 1471 1135 1081 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2141 2141
Size: 6307
SfoI GGC'GCC
Cuts at: 2079 2079
Size: 6307
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6307
SgrAI Cr'CCGG_yG
Cuts at: 1700 2330 1700
Size: 630 5677
SmaI CCC'GGG
Cuts at: 1872 2030 2085 3634 1872
Size: 158 55 1549 4545
Fragments arranged by size:
4545 1549 158 55
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3338 4411 5312 5574 820
Size: 197 926 1395 1073 901 262 1553
Fragments arranged by size:
1553 1395 1073 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4098 493
Size: 3605 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6307
SphI G_CATG'C
Cuts at: 1132 3665 1132
Size: 2533 3774
SspI AAT'ATT
Cuts at: 5 52 4231 5
Size: 47 4179 2081
Fragments arranged by size:
4179 2081 47
StuI AGG'CCT
Cuts at: 1517 2906 1517
Size: 1389 4918
StyI C'CwwG_G
Cuts at: 513 1065 2907 3746 513
Size: 552 1842 839 3074
Fragments arranged by size:
3074 1842 839 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2900 3389 4334 4351 4504 4689 5134
Size: 1255 1320 489 945 17 153 185 445
Cuts at: 5134 325
Size: 1498
Fragments arranged by size:
1498 1320 1255 945 489 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2636 2832 4553
Size: 274 80 33 51 1443 659 196 1721
Cuts at: 4553 6265 96
Size: 1712 138
Fragments arranged by size:
1721 1712 1443 659 274 196 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2762 2794 6267 6299 2762
Size: 32 3473 32 2770
Fragments arranged by size:
3473 2770 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2446 1316
Size: 1130 5177
XbaI T'CTAG_A
Cuts at: 3643 3643
Size: 6307
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2200 2200
Size: 6307
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6307
XmaI C'CCGG_G
Cuts at: 1870 2028 2083 3632 1870
Size: 158 55 1549 4545
Fragments arranged by size:
4545 1549 158 55
XmnI GAAnn'nnTTC
Cuts at: 2096 4436 2096
Size: 2340 3967
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4115 276
Size: 53 83 186 936 2581 2468
Fragments arranged by size:
2581 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I
Bsu36I FalI NruI NsiI PacI PfoI PmlI PspOMI
PspXI PsrI SacII SanDI SrfI SwaI