Restriction map for
pFN21AB7247
(Circular) (Six-base) MAPSORT of: pFN21AB7247.seq Check: 3324 from: 1 to: 6493
pFN21AB7247 6493 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3012 3044 3012
Size: 32 6461
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4303 278
Size: 53 83 186 936 2767 2468
Fragments arranged by size:
2767 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3780 3836 1937
Size: 1843 56 4594
Fragments arranged by size:
4594 1843 56
Acc65I G'GTAC_C
Cuts at: 3814 3814
Size: 6493
AclI AA'CG_TT
Cuts at: 1563 4620 4993 1563
Size: 3057 373 3063
Fragments arranged by size:
3063 3057 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2145 2408 2875 4556 5940 6208 1632
Size: 513 263 467 1681 1384 268 1917
Fragments arranged by size:
1917 1681 1384 513 467 268 263
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6296
AflIII A'CryG_T
Cuts at: 1203 2364 5392 6369 1203
Size: 1161 3028 977 1327
Fragments arranged by size:
3028 1327 1161 977
AgeI A'CCGG_T
Cuts at: 5324 5324
Size: 6493
AhdI GACnn_n'nnGTC
Cuts at: 5222 5222
Size: 6493
AleI CACnn'nnGTG
Cuts at: 1166 2973 1166
Size: 1807 4686
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3076 1184
Size: 1892 4601
AlwNI CAG_nnn'CTG
Cuts at: 3520 4236 5808 3520
Size: 716 1572 4205
Fragments arranged by size:
4205 1572 716
ApaI G_GGCC'C
Cuts at: 2481 2481
Size: 6493
ApaLI G'TGCA_C
Cuts at: 4550 5706 4550
Size: 1156 5337
ApoI r'AATT_y
Cuts at: 1493 3802 4028 4180 6289 1493
Size: 2309 226 152 2109 1697
Fragments arranged by size:
2309 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5047 160
Size: 872 4015 1606
Fragments arranged by size:
4015 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6493
AvaI C'yCGr_G
Cuts at: 1870 1943 2019 2275 3449 3818 1870
Size: 73 76 256 1174 369 4545
Fragments arranged by size:
4545 1174 369 256 76 73
BamHI G'GATC_C
Cuts at: 2370 3823 4190 2370
Size: 1453 367 4673
Fragments arranged by size:
4673 1453 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2312 2962 2975 3426 3814
Size: 325 217 627 525 650 13 451 388
Cuts at: 3814 5269 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 650 627 525 451 388 325
217 13
BanII G_rGCy'C
Cuts at: 1391 2020 2283 2481 3812 1391
Size: 629 263 198 1331 4072
Fragments arranged by size:
4072 1331 629 263 198
BbeI G_GCGC'C
Cuts at: 2316 3430 2316
Size: 1114 5379
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2587 2608 3733 928
Size: 971 688 21 1125 3688
Fragments arranged by size:
3688 1125 971 688 21
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4684 4718 1903
Size: 34 2747 34 3678
Fragments arranged by size:
3678 2747 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4385 5595 4385
Size: 1210 5283
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6493
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2024 2258 3180
Size: 107 122 71 631 605 352 234 922
Cuts at: 3180 3285 3317 3536 5104 136
Size: 105 32 219 1568 1525
Fragments arranged by size:
1568 1525 922 631 605 352 234 219
122 107 105 71 32
BglII A'GATC_T
Cuts at: 3591 6488 3591
Size: 2897 3596
BlpI GC'TnA_GC
Cuts at: 2092 2778 3477 3910 2092
Size: 686 699 433 4675
Fragments arranged by size:
4675 699 686 433
Bme1580I G_kGCm'C
Cuts at: 1790 2481 2980 3185 3583 4554 5710 1790
Size: 691 499 205 398 971 1156 2573
Fragments arranged by size:
2573 1156 971 691 499 398 205
BmgBI CAC'GTC
Cuts at: 1318 2365 1318
Size: 1047 5446
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3284 4224 5182
Size: 416 53 83 399 24 1867 940 958
Cuts at: 5182 442
Size: 1753
Fragments arranged by size:
1867 1753 958 940 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6493
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3221 3524 5153 1239
Size: 1982 303 1629 2579
Fragments arranged by size:
2579 1982 1629 303
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3569 4618 5483 5781 3569
Size: 1049 865 298 4281
Fragments arranged by size:
4281 1049 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2074 2810 5156 882
Size: 617 575 736 2346 2219
Fragments arranged by size:
2346 2219 736 617 575
BsaAI yAC'GTr
Cuts at: 493 1204 2959 4284 493
Size: 711 1755 1325 2702
Fragments arranged by size:
2702 1755 1325 711
BsaBI GATnn'nnATC
Cuts at: 3287 6487 3287
Size: 3200 3293
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2054 2313 2625 3427
Size: 53 83 186 936 521 259 312 802
Cuts at: 3427 4300 4682 5317 275
Size: 873 382 635 1451
Fragments arranged by size:
1451 936 873 802 635 521 382 312
259 186 83 53
BsaWI w'CCGG_w
Cuts at: 2862 4926 5324 5598 5745 6156 2862
Size: 2064 398 274 147 411 3199
Fragments arranged by size:
3199 2064 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3533 3563 3756 3786 1178
Size: 30 165 30 2130 30 193 30 3885
Fragments arranged by size:
3885 2130 193 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2183 3218 1186
Size: 997 1035 4461
Fragments arranged by size:
4461 1035 997
BseYI C'CCAG_C
Cuts at: 2043 3031 3508 5696 6192 2043
Size: 988 477 2188 496 2344
Fragments arranged by size:
2344 2188 988 496 477
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2503 2990 3255 3404 1163
Size: 573 767 487 265 149 4252
Fragments arranged by size:
4252 767 573 487 265 149
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2181 3943 4704 5732 1540
Size: 275 186 180 1762 761 1028 2301
Fragments arranged by size:
2301 1762 1028 761 275 186 180
BsiHKAI G_wGCw'C
Cuts at: 2457 2508 2643 3075 4554 4639 5710 2457
Size: 51 135 432 1479 85 1071 3240
Fragments arranged by size:
3240 1479 1071 432 135 85 51
BsmI GAATG_Cn'
Cuts at: 4004 4097 4004
Size: 93 6400
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2020 2283 2457 2481 2508 2643 2980
Size: 399 230 263 174 24 27 135 337
Cuts at: 2980 3075 3185 3583 3812 4554 4639 5710 1391
Size: 95 110 398 229 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 398 337 263 230
229 174 135 110 95 85 27 24
BspEI T'CCGG_A
Cuts at: 2862 6156 2862
Size: 3294 3199
BspHI T'CATG_A
Cuts at: 4382 6112 4382
Size: 1730 4763
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2730 3012 3044 3848 844
Size: 1886 282 32 804 3489
Fragments arranged by size:
3489 1886 804 282 32
BsrBI CCG'CTC
Cuts at: 1394 1823 2024 2091 2177 4380 1394
Size: 429 201 67 86 2203 3507
Fragments arranged by size:
3507 2203 429 201 86 67
BsrDI GCAATG_nn'
Cuts at: 65 1248 2818 3102 4988 5162 65
Size: 1183 1570 284 1886 174 1396
Fragments arranged by size:
1886 1570 1396 1183 284 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2244 3423 5137 5324 1700
Size: 110 434 1179 1714 187 2869
Fragments arranged by size:
2869 1714 1179 434 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6493
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6493
BssSI C'ACGA_G
Cuts at: 4553 5565 4553
Size: 1012 5481
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2526 3337 794
Size: 1732 811 3950
Fragments arranged by size:
3950 1732 811
BstBI TT'CG_AA
Cuts at: 5297 5297
Size: 6493
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6493
BstXI CCAn_nnnn'nTGG
Cuts at: 2261 3166 2261
Size: 905 5588
BstYI r'GATC_y
Cuts at: 1972 2370 2464 3591 3823 4190 4575 4592 6044
Size: 398 94 1127 232 367 385 17 1452
Cuts at: 6044 6130 6142 6488 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1127 398 385 367 346 232
94 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2011 513
Size: 552 939 7 4995
Fragments arranged by size:
4995 939 552 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3274 3373 487
Size: 683 56 2048 99 3607
Fragments arranged by size:
3607 2048 683 99 56
BtsI GCAGTG_nn'
Cuts at: 2650 4009 4803 4823 2650
Size: 1359 794 20 4320
Fragments arranged by size:
4320 1359 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6493
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6458
DraI TTT'AAA
Cuts at: 3797 3797
Size: 6493
DraIII CAC_nnn'GTG
Cuts at: 2452 2691 2850 3627 3760 2452
Size: 239 159 777 133 5185
Fragments arranged by size:
5185 777 239 159 133
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5500 809
Size: 507 4184 1802
Fragments arranged by size:
4184 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2178 2346 2379
Size: 54 1425 50 275 18 348 168 33
Cuts at: 2379 2712 2739 3309 3318 3940 4829 6458 8
Size: 333 27 570 9 622 889 1629 43
Fragments arranged by size:
1629 1425 889 622 570 348 333 275
168 54 50 43 33 27 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2178 3940 1537
Size: 275 366 1762 4090
Fragments arranged by size:
4090 1762 366 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2276 2513 3080 3407 3605 4423 1353
Size: 923 237 567 327 198 818 3423
Fragments arranged by size:
3423 923 818 567 327 237 198
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5080 5454 5600 387
Size: 1064 138 3491 374 146 1280
Fragments arranged by size:
3491 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2145 2408 2875 3221 3524 4556 5153
Size: 393 513 263 467 346 303 1032 597
Cuts at: 5153 5940 6208 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1032 787 597 513 467 393 346
303 268 263
EcoNI CCTnn'n_nnAGG
Cuts at: 3773 3773
Size: 6493
EcoO109I rG'GnC_Cy
Cuts at: 2392 2477 2478 3415 3454 3517 2392
Size: 85 1 937 39 63 5368
Fragments arranged by size:
5368 937 85 63 39 1
EcoRI G'AATT_C
Cuts at: 3802 4180 3802
Size: 378 6115
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2798 2798
Size: 6493
FspI TGC'GCA
Cuts at: 2034 4999 2034
Size: 2965 3528
HaeII r_GCGC'y
Cuts at: 1678 2099 2316 3328 3430 5640 1678
Size: 421 217 1012 102 2210 2531
Fragments arranged by size:
2531 2210 1012 421 217 102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2070 2102 5134 5166 5208
Size: 32 135 32 498 32 3032 32 42
Cuts at: 5208 5240 1373
Size: 32 2626
Fragments arranged by size:
3032 2626 498 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3718 3837 4083 669
Size: 988 281 1780 119 246 3079
Fragments arranged by size:
3079 1780 988 281 246 119
HindIII A'AGCT_T
Cuts at: 748 2856 748
Size: 2108 4385
HpaI GTT'AAC
Cuts at: 4083 4083
Size: 6493
KasI G'GCGC_C
Cuts at: 2312 3426 2312
Size: 1114 5379
KpnI G_GTAC'C
Cuts at: 3818 3818
Size: 6493
MfeI C'AATT_G
Cuts at: 4092 4092
Size: 6493
MluI A'CGCG_T
Cuts at: 6369 6369
Size: 6493
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5607 5791 1305
Size: 4302 184 2007
Fragments arranged by size:
4302 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2381 2714 3311 3320 10
Size: 54 1425 343 549 333 597 9 3183
Fragments arranged by size:
3183 1425 597 549 343 333 54 9
MslI CAynn'nnrTG
Cuts at: 518 1166 2609 2792 2973 4451 4810 4969 518
Size: 648 1443 183 181 1478 359 159 2042
Fragments arranged by size:
2042 1478 1443 648 359 183 181 159
MspA1I CmG'CkG
Cuts at: 1606 2013 2043 2517 2571 2584 3028 3526 3909
Size: 407 30 474 54 13 444 498 383
Cuts at: 3909 4586 5734 5979 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 498 474 444 407 383
245 54 30 13
NarI GG'CG_CC
Cuts at: 2313 3427 2313
Size: 1114 5379
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5002
Fragments arranged by size:
5002 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6493
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6493
NotI GC'GGCC_GC
Cuts at: 2178 3940 2178
Size: 1762 4731
NspI r_CATG'y
Cuts at: 1132 1229 3754 3851 5396 1132
Size: 97 2525 97 1545 2229
Fragments arranged by size:
2525 2229 1545 97 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6493
PciI A'CATG_T
Cuts at: 5392 5392
Size: 6493
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6493
PmeI GTTT'AAAC
Cuts at: 3797 3797
Size: 6493
PmlI CAC'GTG
Cuts at: 2959 2959
Size: 6493
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4562 4594 6075 6107 4562
Size: 32 1481 32 4948
Fragments arranged by size:
4948 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2392 2392
Size: 6493
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6493
PsiI TTA'TAA
Cuts at: 4063 4063
Size: 6493
PspOMI G'GGCC_C
Cuts at: 2477 2477
Size: 6493
PstI C_TGCA'G
Cuts at: 830 2327 3384 3525 3845 830
Size: 1497 1057 141 320 3478
Fragments arranged by size:
3478 1497 1057 320 141
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6493
PvuII CAG'CTG
Cuts at: 2517 2584 2517
Size: 67 6426
SacII CC_GC'GG
Cuts at: 2014 2014
Size: 6493
SalI G'TCGA_C
Cuts at: 1936 3835 1936
Size: 1899 4594
SapI GCTCTTCn'nnn_
Cuts at: 2513 3080 2513
Size: 567 5926
SbfI CC_TGCA'GG
Cuts at: 3845 3845
Size: 6493
ScaI AGT'ACT
Cuts at: 2903 3116 4741 6453 2903
Size: 213 1625 1712 2943
Fragments arranged by size:
2943 1712 1625 213
SfcI C'TryA_G
Cuts at: 826 1045 2323 3380 3521 3553 3841 4976 5657
Size: 219 1278 1057 141 32 288 1135 681
Cuts at: 5657 5848 826
Size: 191 1471
Fragments arranged by size:
1471 1278 1135 1057 681 288 219 191
141 32
SfiI GGCCn_nnn'nGGCC
Cuts at: 3317 3536 3317
Size: 219 6274
SfoI GGC'GCC
Cuts at: 2314 3428 2314
Size: 1114 5379
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6493
SgrAI Cr'CCGG_yG
Cuts at: 1700 3423 1700
Size: 1723 4770
SmaI CCC'GGG
Cuts at: 1872 3820 1872
Size: 1948 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3584 4597 5498 5760 820
Size: 197 926 1641 1013 901 262 1553
Fragments arranged by size:
1641 1553 1013 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4284 493
Size: 3791 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6493
SphI G_CATG'C
Cuts at: 1132 3754 3851 1132
Size: 2622 97 3774
Fragments arranged by size:
3774 2622 97
SspI AAT'ATT
Cuts at: 5 52 4417 5
Size: 47 4365 2081
Fragments arranged by size:
4365 2081 47
StuI AGG'CCT
Cuts at: 1517 3768 1517
Size: 2251 4242
StyI C'CwwG_G
Cuts at: 513 1065 2004 3932 513
Size: 552 939 1928 3074
Fragments arranged by size:
3074 1928 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2056 2297 2348 2476 2626 2963 3254
Size: 1255 476 241 51 128 150 337 291
Cuts at: 3254 4520 4537 4690 4875 5320 325
Size: 1266 17 153 185 445 1498
Fragments arranged by size:
1498 1266 1255 476 445 337 291 241
185 153 150 128 51 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2901 3114 4739
Size: 274 80 33 51 1443 924 213 1625
Cuts at: 4739 6451 96
Size: 1712 138
Fragments arranged by size:
1712 1625 1443 924 274 213 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3756 3788 6453 6485 3756
Size: 32 2665 32 3764
Fragments arranged by size:
3764 2665 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2591 3221 1316
Size: 1275 630 4588
Fragments arranged by size:
4588 1275 630
XbaI T'CTAG_A
Cuts at: 3829 3829
Size: 6493
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3163 3196 3163
Size: 33 6460
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6493
XmaI C'CCGG_G
Cuts at: 1870 3818 1870
Size: 1948 4545
XmnI GAAnn'nnTTC
Cuts at: 3401 4622 3401
Size: 1221 5272
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4301 276
Size: 53 83 186 936 2767 2468
Fragments arranged by size:
2767 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI
FspAI NaeI NgoMIV NruI NsiI PacI PfoI PspXI
PsrI RsrII SacI SanDI SexAI SrfI SwaI