Restriction map for
pFN21AB7246
(Circular) (Six-base) MAPSORT of: pFN21AB7246.seq Check: 1489 from: 1 to: 6265
pFN21AB7246 6265 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4075 278
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3608 1937
Size: 1671 4594
Acc65I G'GTAC_C
Cuts at: 3586 3586
Size: 6265
AclI AA'CG_TT
Cuts at: 1563 4392 4765 1563
Size: 2829 373 3063
Fragments arranged by size:
3063 2829 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2023 2077 2095 2312 2821 2921 3542 3566
Size: 391 54 18 217 509 100 621 24
Cuts at: 3566 4328 5712 5980 1632
Size: 762 1384 268 1917
Fragments arranged by size:
1917 1384 762 621 509 391 268 217
100 54 24 18
AfeI AGC'GCT
Cuts at: 2044 3325 3496 2044
Size: 1281 171 4813
Fragments arranged by size:
4813 1281 171
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6068
AflIII A'CryG_T
Cuts at: 1203 2793 5164 6141 1203
Size: 1590 2371 977 1327
Fragments arranged by size:
2371 1590 1327 977
AgeI A'CCGG_T
Cuts at: 5096 5096
Size: 6265
AhdI GACnn_n'nnGTC
Cuts at: 4994 4994
Size: 6265
AleI CACnn'nnGTG
Cuts at: 1166 2625 2798 1166
Size: 1459 173 4633
Fragments arranged by size:
4633 1459 173
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6265
AlwNI CAG_nnn'CTG
Cuts at: 2278 2476 3019 3169 3199 4008 5580 2278
Size: 198 543 150 30 809 1572 2963
Fragments arranged by size:
2963 1572 809 543 198 150 30
ApaI G_GGCC'C
Cuts at: 2029 2029
Size: 6265
ApaLI G'TGCA_C
Cuts at: 3286 4322 5478 3286
Size: 1036 1156 4073
Fragments arranged by size:
4073 1156 1036
ApoI r'AATT_y
Cuts at: 1493 3574 3800 3952 6061 1493
Size: 2081 226 152 2109 1697
Fragments arranged by size:
2109 2081 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4819 160
Size: 872 3787 1606
Fragments arranged by size:
3787 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
AvaI C'yCGr_G
Cuts at: 1870 1943 3590 1870
Size: 73 1647 4545
Fragments arranged by size:
4545 1647 73
BamHI G'GATC_C
Cuts at: 3595 3962 3595
Size: 367 5898
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2142 3586 5041 618
Size: 325 217 627 355 1444 1455 1842
Fragments arranged by size:
1842 1455 1444 627 355 325 217
BanII G_rGCy'C
Cuts at: 1391 2029 2229 2551 3449 3584 1391
Size: 638 200 322 898 135 4072
Fragments arranged by size:
4072 898 638 322 200 135
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3533 3554 928
Size: 971 1634 21 3639
Fragments arranged by size:
3639 1634 971 21
BbvCI CC'TCA_GC
Cuts at: 2898 2904 2898
Size: 6 6259
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4456 4490 1903
Size: 34 2519 34 3678
Fragments arranged by size:
3678 2519 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4157 5367 4157
Size: 1210 5055
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6265
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2151 2472 4876
Size: 107 122 71 631 605 479 321 2404
Cuts at: 4876 136
Size: 1525
Fragments arranged by size:
2404 1525 631 605 479 321 122 107
71
BglII A'GATC_T
Cuts at: 2988 6260 2988
Size: 3272 2993
BlpI GC'TnA_GC
Cuts at: 2050 3084 3682 2050
Size: 1034 598 4633
Fragments arranged by size:
4633 1034 598
Bme1580I G_kGCm'C
Cuts at: 1790 2029 2240 3290 4326 5482 1790
Size: 239 211 1050 1036 1156 2573
Fragments arranged by size:
2573 1156 1050 1036 239 211
BmgBI CAC'GTC
Cuts at: 1318 2796 1318
Size: 1478 4787
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2305 3996 4954
Size: 416 53 83 399 24 888 1691 958
Cuts at: 4954 442
Size: 1753
Fragments arranged by size:
1753 1691 958 888 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6265
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2747 2747
Size: 6265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2420 2441 2498 2516 2543 2732 2759 3158
Size: 1181 21 57 18 27 189 27 399
Cuts at: 3158 3275 3357 3444 3475 3487 4925 1239
Size: 117 82 87 31 12 1438 2579
Fragments arranged by size:
2579 1438 1181 399 189 117 87 82
57 31 27 27 21 18 12
Bpu10I CC'TnA_GC
Cuts at: 2510 2684 2898 2904 3117 2510
Size: 174 214 6 213 5658
Fragments arranged by size:
5658 214 213 174 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2053 2642 2657 3026 3092 4390 5255 5553 2053
Size: 589 15 369 66 1298 865 298 2765
Fragments arranged by size:
2765 1298 865 589 369 298 66 15
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2330 4928 882
Size: 617 831 2598 2219
Fragments arranged by size:
2598 2219 831 617
BsaAI yAC'GTr
Cuts at: 493 1204 4056 493
Size: 711 2852 2702
Fragments arranged by size:
2852 2702 711
BsaBI GATnn'nnATC
Cuts at: 2300 6259 2300
Size: 3959 2306
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4072 4454 5089 275
Size: 53 83 186 936 2539 382 635 1451
Fragments arranged by size:
2539 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4698 5096 5370 5517 5928 4698
Size: 398 274 147 411 5035
Fragments arranged by size:
5035 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2414 2444 2576 2582 2606
Size: 30 165 30 1011 30 132 6 24
Cuts at: 2606 2612 3352 3382 1178
Size: 6 740 30 4061
Fragments arranged by size:
4061 1011 740 165 132 30 30 30
30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2600 2603 2624 2627 2888 3278 3298 3354
Size: 1414 3 21 3 261 390 20 56
Cuts at: 3354 1186
Size: 4097
Fragments arranged by size:
4097 1414 390 261 56 21 20 3
3
BseYI C'CCAG_C
Cuts at: 2188 2764 2949 5468 5964 2188
Size: 576 185 2519 496 2489
Fragments arranged by size:
2519 2489 576 496 185
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3289 3391 1163
Size: 573 1553 102 4037
Fragments arranged by size:
4037 1553 573 102
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3715 4476 5504 1540
Size: 275 186 1714 761 1028 2301
Fragments arranged by size:
2301 1714 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3209 3290 3365 4326 4411 5482 3209
Size: 81 75 961 85 1071 3992
Fragments arranged by size:
3992 1071 961 85 81 75
BsmI GAATG_Cn'
Cuts at: 3776 3869 3776
Size: 93 6172
BsmBI CGTCTCn'nnnn_
Cuts at: 2417 2417
Size: 6265
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2029 2229 2240 2551 3209 3290 3365
Size: 399 239 200 11 311 658 81 75
Cuts at: 3365 3449 3584 4326 4411 5482 1391
Size: 84 135 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 658 399 311 239 200
135 85 84 81 75 11
BspEI T'CCGG_A
Cuts at: 5928 5928
Size: 6265
BspHI T'CATG_A
Cuts at: 4154 5884 4154
Size: 1730 4535
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3620 844
Size: 2776 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4152 1394
Size: 429 2329 3507
Fragments arranged by size:
3507 2329 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4760 4934 65
Size: 1183 3512 174 1396
Fragments arranged by size:
3512 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4909 5096 1700
Size: 110 3099 187 2869
Fragments arranged by size:
3099 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6265
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6265
BssSI C'ACGA_G
Cuts at: 2157 4325 5337 2157
Size: 2168 1012 3085
Fragments arranged by size:
3085 2168 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2440 794
Size: 1646 4619
BstBI TT'CG_AA
Cuts at: 5069 5069
Size: 6265
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6265
BstXI CCAn_nnnn'nTGG
Cuts at: 2957 2957
Size: 6265
BstYI r'GATC_y
Cuts at: 1972 2988 3595 3962 4347 4364 5816 5902 5914
Size: 1016 607 367 385 17 1452 86 12
Cuts at: 5914 6260 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1016 607 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2811 513
Size: 552 1746 3967
Fragments arranged by size:
3967 1746 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5526
Fragments arranged by size:
5526 683 56
BtsI GCAGTG_nn'
Cuts at: 3781 4575 4595 3781
Size: 794 20 5451
Fragments arranged by size:
5451 794 20
ClaI AT'CG_AT
Cuts at: 1554 2304 1554
Size: 750 5515
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6230
DraI TTT'AAA
Cuts at: 3569 3569
Size: 6265
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2651 3535 5272 809
Size: 507 1335 884 1737 1802
Fragments arranged by size:
1802 1737 1335 884 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3156 3712 4601
Size: 54 1425 50 275 18 1326 556 889
Cuts at: 4601 6230 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1326 889 556 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3712 1537
Size: 275 1900 4090
Fragments arranged by size:
4090 1900 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2754 4195 1353
Size: 1401 1441 3423
Fragments arranged by size:
3423 1441 1401
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4852 5226 5372 387
Size: 1064 138 3263 374 146 1280
Fragments arranged by size:
3263 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2023 2077 2095 2312 2420 2441 2498
Size: 393 391 54 18 217 108 21 57
Cuts at: 2498 2516 2543 2732 2759 2821 2921 3158 3275
Size: 18 27 189 27 62 100 237 117
Cuts at: 3275 3357 3444 3475 3487 3542 3566 4328 4925
Size: 82 87 31 12 55 24 762 597
Cuts at: 4925 5712 5980 1239
Size: 787 268 1524
Fragments arranged by size:
1524 787 762 597 393 391 268 237
217 189 117 108 100 87 82 62
57 55 54 31 27 27 24 21
18 18 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2660 2660
Size: 6265
EcoO109I rG'GnC_Cy
Cuts at: 2416 2473 3399 3457 2416
Size: 57 926 58 5224
Fragments arranged by size:
5224 926 58 57
EcoRI G'AATT_C
Cuts at: 3574 3952 3574
Size: 378 5887
FspI TGC'GCA
Cuts at: 4771 4771
Size: 6265
HaeII r_GCGC'y
Cuts at: 1678 2046 3327 3498 5412 1678
Size: 368 1281 171 1914 2531
Fragments arranged by size:
2531 1914 1281 368 171
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2582 2614 3461 3493 4906
Size: 32 135 32 1010 32 847 32 1413
Cuts at: 4906 4938 4980 5012 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1413 1010 847 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3609 3855 669
Size: 988 281 1671 246 3079
Fragments arranged by size:
3079 1671 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3212 748
Size: 2464 3801
HpaI GTT'AAC
Cuts at: 3855 3855
Size: 6265
KpnI G_GTAC'C
Cuts at: 3590 3590
Size: 6265
MfeI C'AATT_G
Cuts at: 3864 3864
Size: 6265
MluI A'CGCG_T
Cuts at: 6141 6141
Size: 6265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2679 3456 5379 5563 1305
Size: 1374 777 1923 184 2007
Fragments arranged by size:
2007 1923 1374 777 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3158 10
Size: 54 1425 343 1326 3117
Fragments arranged by size:
3117 1425 1326 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2625 2751 2798 4223 4582 4741 518
Size: 648 1459 126 47 1425 359 159 2042
Fragments arranged by size:
2042 1459 1425 648 359 159 126 47
MspA1I CmG'CkG
Cuts at: 1606 2521 3507 3681 4358 5506 5751 1606
Size: 915 986 174 677 1148 245 2120
Fragments arranged by size:
2120 1148 986 915 677 245 174
NcoI C'CATG_G
Cuts at: 513 1065 2811 513
Size: 552 1746 3967
Fragments arranged by size:
3967 1746 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6265
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6265
NotI GC'GGCC_GC
Cuts at: 3712 3712
Size: 6265
NspI r_CATG'y
Cuts at: 1132 1229 3623 5168 1132
Size: 97 2394 1545 2229
Fragments arranged by size:
2394 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3395 1348
Size: 2047 4218
PciI A'CATG_T
Cuts at: 5164 5164
Size: 6265
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6265
PfoI T'CCnGG_A
Cuts at: 2397 2418 2397
Size: 21 6244
PmeI GTTT'AAAC
Cuts at: 3569 3569
Size: 6265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4334 4366 5847 5879 4334
Size: 32 1481 32 4720
Fragments arranged by size:
4720 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2416 3399 3457 2416
Size: 983 58 5224
Fragments arranged by size:
5224 983 58
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6265
PsiI TTA'TAA
Cuts at: 3835 3835
Size: 6265
PspOMI G'GGCC_C
Cuts at: 2025 2025
Size: 6265
PstI C_TGCA'G
Cuts at: 830 2445 3024 3066 3617 830
Size: 1615 579 42 551 3478
Fragments arranged by size:
3478 1615 579 551 42
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6265
PvuII CAG'CTG
Cuts at: 2521 3507 2521
Size: 986 5279
SalI G'TCGA_C
Cuts at: 1936 3607 1936
Size: 1671 4594
SapI GCTCTTCn'nnn_
Cuts at: 2754 2754
Size: 6265
SbfI CC_TGCA'GG
Cuts at: 3066 3617 3066
Size: 551 5714
ScaI AGT'ACT
Cuts at: 4513 6225 4513
Size: 1712 4553
SfcI C'TryA_G
Cuts at: 826 1045 2441 3020 3062 3613 4748 5429 5620
Size: 219 1396 579 42 551 1135 681 191
Cuts at: 5620 826
Size: 1471
Fragments arranged by size:
1471 1396 1135 681 579 551 219 191
42
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6265
SmaI CCC'GGG
Cuts at: 1872 3592 1872
Size: 1720 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2068 2636 2657 3041 3107 4369
Size: 197 926 125 568 21 384 66 1262
Cuts at: 4369 5270 5532 820
Size: 901 262 1553
Fragments arranged by size:
1553 1262 926 901 568 384 262 197
125 66 21
SnaBI TAC'GTA
Cuts at: 493 4056 493
Size: 3563 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6265
SphI G_CATG'C
Cuts at: 1132 3623 1132
Size: 2491 3774
SspI AAT'ATT
Cuts at: 5 52 4189 5
Size: 47 4137 2081
Fragments arranged by size:
4137 2081 47
StuI AGG'CCT
Cuts at: 1517 2495 2570 2909 1517
Size: 978 75 339 4873
Fragments arranged by size:
4873 978 339 75
StyI C'CwwG_G
Cuts at: 513 1065 2811 3123 3704 513
Size: 552 1746 312 581 3074
Fragments arranged by size:
3074 1746 581 552 312
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4292 4309 4462 4647 5092 325
Size: 1255 2712 17 153 185 445 1498
Fragments arranged by size:
2712 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4511 6223 96
Size: 274 80 33 51 1443 2534 1712 138
Fragments arranged by size:
2534 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2414 2446 3352 3384 6225 6257 2414
Size: 32 906 32 2841 32 2422
Fragments arranged by size:
2841 2422 906 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6265
XbaI T'CTAG_A
Cuts at: 3601 3601
Size: 6265
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3166 3439 3166
Size: 273 5992
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6265
XmaI C'CCGG_G
Cuts at: 1870 3590 1870
Size: 1720 4545
XmnI GAAnn'nnTTC
Cuts at: 4394 4394
Size: 6265
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4073 276
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI