Restriction map for
pFN21AB7244
(Circular) (Six-base) MAPSORT of: pFN21AB7244.seq Check: 8794 from: 1 to: 5797
pFN21AB7244 5797 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2265 3607 278
Size: 53 83 186 936 729 1342 2468
Fragments arranged by size:
2468 1342 936 729 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3140 1937
Size: 1203 4594
Acc65I G'GTAC_C
Cuts at: 2555 3118 2555
Size: 563 5234
AclI AA'CG_TT
Cuts at: 1563 3924 4297 1563
Size: 2361 373 3063
Fragments arranged by size:
3063 2361 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3860 5244 5512 1632
Size: 2228 1384 268 1917
Fragments arranged by size:
2228 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5600
AflIII A'CryG_T
Cuts at: 1203 4696 5673 1203
Size: 3493 977 1327
Fragments arranged by size:
3493 1327 977
AgeI A'CCGG_T
Cuts at: 4628 4628
Size: 5797
AhdI GACnn_n'nnGTC
Cuts at: 4526 4526
Size: 5797
AleI CACnn'nnGTG
Cuts at: 1166 2621 1166
Size: 1455 4342
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2534 1184
Size: 1350 4447
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2000 2032 2033 2065 2294 2326 2000
Size: 32 1 32 229 32 5471
Fragments arranged by size:
5471 229 32 32 32 1
AlwNI CAG_nnn'CTG
Cuts at: 3540 5112 3540
Size: 1572 4225
ApaI G_GGCC'C
Cuts at: 2119 2119
Size: 5797
ApaLI G'TGCA_C
Cuts at: 2429 3854 5010 2429
Size: 1425 1156 3216
Fragments arranged by size:
3216 1425 1156
ApoI r'AATT_y
Cuts at: 1493 3106 3332 3484 5593 1493
Size: 1613 226 152 2109 1697
Fragments arranged by size:
2109 1697 1613 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4351 160
Size: 872 3319 1606
Fragments arranged by size:
3319 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
AvaI C'yCGr_G
Cuts at: 1870 1943 2053 2118 3122 1870
Size: 73 110 65 1004 4545
Fragments arranged by size:
4545 1004 110 73 65
BamHI G'GATC_C
Cuts at: 3021 3127 3494 3021
Size: 106 367 5324
Fragments arranged by size:
5324 367 106
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2101 2131 2454 2555 2597
Size: 325 217 627 314 30 323 101 42
Cuts at: 2597 2981 3118 4573 618
Size: 384 137 1455 1842
Fragments arranged by size:
1842 1455 627 384 325 323 314 217
137 101 42 30
BanII G_rGCy'C
Cuts at: 1391 2013 2119 2444 2583 2844 2978 3116 1391
Size: 622 106 325 139 261 134 138 4072
Fragments arranged by size:
4072 622 325 261 139 138 134 106
BbeI G_GCGC'C
Cuts at: 2105 2458 2601 2105
Size: 353 143 5301
Fragments arranged by size:
5301 353 143
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2225 2377 2596 928
Size: 971 326 152 219 4129
Fragments arranged by size:
4129 971 326 219 152
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3988 4022 1903
Size: 34 2051 34 3678
Fragments arranged by size:
3678 2051 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3689 4899 3689
Size: 1210 4587
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5797
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2477 2587 4408
Size: 107 122 71 631 605 805 110 1821
Cuts at: 4408 136
Size: 1525
Fragments arranged by size:
1821 1525 805 631 605 122 110 107
71
BglII A'GATC_T
Cuts at: 5792 5792
Size: 5797
BlpI GC'TnA_GC
Cuts at: 2438 3214 2438
Size: 776 5021
Bme1580I G_kGCm'C
Cuts at: 1790 2119 2136 2433 3858 5014 1790
Size: 329 17 297 1425 1156 2573
Fragments arranged by size:
2573 1425 1156 329 297 17
BmgBI CAC'GTC
Cuts at: 1318 2542 1318
Size: 1224 4573
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3528 4486 442
Size: 416 53 83 399 24 2111 958 1753
Fragments arranged by size:
2111 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5797
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2863 2863
Size: 5797
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2088 4457 1239
Size: 849 2369 2579
Fragments arranged by size:
2579 2369 849
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3922 4787 5085 3922
Size: 865 298 4634
Fragments arranged by size:
4634 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4460 882
Size: 617 2961 2219
Fragments arranged by size:
2961 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2194 3588 493
Size: 711 990 1394 2702
Fragments arranged by size:
2702 1394 990 711
BsaBI GATnn'nnATC
Cuts at: 5791 5791
Size: 5797
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2102 2262 2364 2455
Size: 53 83 186 936 569 160 102 91
Cuts at: 2455 2598 2997 3604 3986 4621 275
Size: 143 399 607 382 635 1451
Fragments arranged by size:
1451 936 635 607 569 399 382 186
160 143 102 91 83 53
BsaWI w'CCGG_w
Cuts at: 4230 4628 4902 5049 5460 4230
Size: 398 274 147 411 4567
Fragments arranged by size:
4567 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2000 2030 2033 2063 1178
Size: 30 165 30 597 30 3 30 4912
Fragments arranged by size:
4912 597 165 30 30 30 30 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2038 1186
Size: 852 4945
BseYI C'CCAG_C
Cuts at: 2020 2033 2167 2275 2626 5000 5496 2020
Size: 13 134 108 351 2374 496 2321
Fragments arranged by size:
2374 2321 496 351 134 108 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2564 1163
Size: 573 828 4396
Fragments arranged by size:
4396 828 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2163 2622 3247 4008 5036 1540
Size: 275 186 162 459 625 761 1028 2301
Fragments arranged by size:
2301 1028 761 625 459 275 186 162
BsiHKAI G_wGCw'C
Cuts at: 2433 2444 3858 3943 5014 2433
Size: 11 1414 85 1071 3216
Fragments arranged by size:
3216 1414 1071 85 11
BsmI GAATG_Cn'
Cuts at: 3308 3401 3308
Size: 93 5704
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2119 2136 2433 2444 2583 2844
Size: 399 223 106 17 297 11 139 261
Cuts at: 2844 2978 3116 3858 3943 5014 1391
Size: 134 138 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 297 261 223 139
138 134 106 85 17 11
BspEI T'CCGG_A
Cuts at: 5460 5460
Size: 5797
BspHI T'CATG_A
Cuts at: 2349 2400 3686 5416 2349
Size: 51 1286 1730 2730
Fragments arranged by size:
2730 1730 1286 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2154 2567 2676 3152 844
Size: 1310 413 109 476 3489
Fragments arranged by size:
3489 1310 476 413 109
BsrBI CCG'CTC
Cuts at: 1394 1823 2524 2734 3684 1394
Size: 429 701 210 950 3507
Fragments arranged by size:
3507 950 701 429 210
BsrDI GCAATG_nn'
Cuts at: 65 1248 4292 4466 65
Size: 1183 3044 174 1396
Fragments arranged by size:
3044 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2098 2852 4441 4628 1700
Size: 110 288 754 1589 187 2869
Fragments arranged by size:
2869 1589 754 288 187 110
BsrGI T'GTAC_A
Cuts at: 96 2268 96
Size: 2172 3625
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5797
BssSI C'ACGA_G
Cuts at: 3857 4869 3857
Size: 1012 4785
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5797
BstBI TT'CG_AA
Cuts at: 4601 4601
Size: 5797
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5797
BstXI CCAn_nnnn'nTGG
Cuts at: 3094 3094
Size: 5797
BstYI r'GATC_y
Cuts at: 1972 3021 3127 3494 3879 3896 5348 5434 5446
Size: 1049 106 367 385 17 1452 86 12
Cuts at: 5446 5792 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1049 385 367 346 106 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2487 2583 2823 3000 513
Size: 552 939 483 96 240 177 3310
Fragments arranged by size:
3310 939 552 483 240 177 96
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5058
Fragments arranged by size:
5058 683 56
BtsI GCAGTG_nn'
Cuts at: 2259 2430 3313 4107 4127 2259
Size: 171 883 794 20 3929
Fragments arranged by size:
3929 883 794 171 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5797
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5762
DraI TTT'AAA
Cuts at: 3101 3101
Size: 5797
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4804 809
Size: 507 3488 1802
Fragments arranged by size:
3488 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2160 2256 2292
Size: 54 1425 50 275 18 330 96 36
Cuts at: 2292 2469 2573 2619 2820 3244 4133 5762 8
Size: 177 104 46 201 424 889 1629 43
Fragments arranged by size:
1629 1425 889 424 330 275 201 177
104 96 54 50 46 43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 2160 2619 3244 1537
Size: 275 348 459 625 4090
Fragments arranged by size:
4090 625 459 348 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3727 1353
Size: 2374 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2839 2927 4384 4758 4904 387
Size: 1064 138 1250 88 1457 374 146 1280
Fragments arranged by size:
1457 1280 1250 1064 374 146 138 88
EcoICRI GAG'CTC
Cuts at: 2442 2442
Size: 5797
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2088 3860 4457 5244 5512 1239
Size: 393 456 1772 597 787 268 1524
Fragments arranged by size:
1772 1524 787 597 456 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2969 2969
Size: 5797
EcoO109I rG'GnC_Cy
Cuts at: 2115 2497 2115
Size: 382 5415
EcoRI G'AATT_C
Cuts at: 3106 3484 3106
Size: 378 5419
FspI TGC'GCA
Cuts at: 4303 4303
Size: 5797
HaeII r_GCGC'y
Cuts at: 1678 2105 2458 2487 2601 2728 2831 4944 1678
Size: 427 353 29 114 127 103 2113 2531
Fragments arranged by size:
2531 2113 427 353 127 114 103 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4438 4470 4512 4544 1373
Size: 32 135 32 2866 32 42 32 2626
Fragments arranged by size:
2866 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2368 2806 2815 3141 3387 669
Size: 988 281 430 438 9 326 246 3079
Fragments arranged by size:
3079 988 438 430 326 281 246 9
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5797
HpaI GTT'AAC
Cuts at: 3387 3387
Size: 5797
KasI G'GCGC_C
Cuts at: 2101 2454 2597 2101
Size: 353 143 5301
Fragments arranged by size:
5301 353 143
KpnI G_GTAC'C
Cuts at: 2559 3122 2559
Size: 563 5234
MfeI C'AATT_G
Cuts at: 3396 3396
Size: 5797
MluI A'CGCG_T
Cuts at: 5673 5673
Size: 5797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4911 5095 1305
Size: 3606 184 2007
Fragments arranged by size:
3606 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2294 2471 10
Size: 54 1425 343 462 177 3336
Fragments arranged by size:
3336 1425 462 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2192 2405 2621 2678 3755 4114 4273
Size: 648 1026 213 216 57 1077 359 159
Cuts at: 4273 518
Size: 2042
Fragments arranged by size:
2042 1077 1026 648 359 216 213 159
57
MspA1I CmG'CkG
Cuts at: 1606 2037 2437 2451 2489 2571 2644 2895 3213
Size: 431 400 14 38 82 73 251 318
Cuts at: 3213 3890 5038 5283 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 431 400 318 251 245
82 73 38 14
NaeI GCC'GGC
Cuts at: 2100 2854 2100
Size: 754 5043
NarI GG'CG_CC
Cuts at: 2102 2455 2598 2102
Size: 353 143 5301
Fragments arranged by size:
5301 353 143
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4306
Fragments arranged by size:
4306 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5797
NgoMIV G'CCGG_C
Cuts at: 2098 2852 2098
Size: 754 5043
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5797
NotI GC'GGCC_GC
Cuts at: 3244 3244
Size: 5797
NspI r_CATG'y
Cuts at: 1132 1229 2275 2515 3155 4700 1132
Size: 97 1046 240 640 1545 2229
Fragments arranged by size:
2229 1545 1046 640 240 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5797
PciI A'CATG_T
Cuts at: 4696 4696
Size: 5797
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5797
PmeI GTTT'AAAC
Cuts at: 3101 3101
Size: 5797
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2000 2032 2033 2065 3866 3898 5379 5411 2000
Size: 32 1 32 1801 32 1481 32 2386
Fragments arranged by size:
2386 1801 1481 32 32 32 32 1
PpuMI rG'GwC_Cy
Cuts at: 2497 2497
Size: 5797
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5797
PsiI TTA'TAA
Cuts at: 3367 3367
Size: 5797
PspOMI G'GGCC_C
Cuts at: 2115 2115
Size: 5797
PstI C_TGCA'G
Cuts at: 830 3149 830
Size: 2319 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5797
PvuII CAG'CTG
Cuts at: 2037 2571 2895 2037
Size: 534 324 4939
Fragments arranged by size:
4939 534 324
SacI G_AGCT'C
Cuts at: 2444 2444
Size: 5797
SacII CC_GC'GG
Cuts at: 2490 2490
Size: 5797
SalI G'TCGA_C
Cuts at: 1936 3139 1936
Size: 1203 4594
SbfI CC_TGCA'GG
Cuts at: 3149 3149
Size: 5797
ScaI AGT'ACT
Cuts at: 4045 5757 4045
Size: 1712 4085
SfcI C'TryA_G
Cuts at: 826 1045 3145 4280 4961 5152 826
Size: 219 2100 1135 681 191 1471
Fragments arranged by size:
2100 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2103 2456 2599 2103
Size: 353 143 5301
Fragments arranged by size:
5301 353 143
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5797
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5797
SmaI CCC'GGG
Cuts at: 1872 2055 2120 3124 1872
Size: 183 65 1004 4545
Fragments arranged by size:
4545 1004 183 65
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3901 4802 5064 820
Size: 197 926 1958 901 262 1553
Fragments arranged by size:
1958 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3588 493
Size: 3095 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5797
SphI G_CATG'C
Cuts at: 1132 3155 1132
Size: 2023 3774
SrfI GCCC'GGGC
Cuts at: 2120 2120
Size: 5797
SspI AAT'ATT
Cuts at: 5 52 3721 5
Size: 47 3669 2081
Fragments arranged by size:
3669 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5797
StyI C'CwwG_G
Cuts at: 513 1065 2004 2985 3236 513
Size: 552 939 981 251 3074
Fragments arranged by size:
3074 981 939 552 251
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3824 3841 3994 4179 4624 325
Size: 1255 2244 17 153 185 445 1498
Fragments arranged by size:
2244 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2268 4043 5755
Size: 274 80 33 51 1443 291 1775 1712
Cuts at: 5755 96
Size: 138
Fragments arranged by size:
1775 1712 1443 291 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5757 5789 5757
Size: 32 5765
Tth111I GACn'n_nGTC
Cuts at: 1316 2363 1316
Size: 1047 4750
XbaI T'CTAG_A
Cuts at: 3133 3133
Size: 5797
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5797
XmaI C'CCGG_G
Cuts at: 1870 2053 2118 3122 1870
Size: 183 65 1004 4545
Fragments arranged by size:
4545 1004 183 65
XmnI GAAnn'nnTTC
Cuts at: 3926 3926
Size: 5797
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2263 3605 276
Size: 53 83 186 936 729 1342 2468
Fragments arranged by size:
2468 1342 936 729 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI
RsrII SanDI SapI SexAI SfiI SwaI XcmI