Restriction map for
pFN21AB7236
(Circular) (Six-base) MAPSORT of: pFN21AB7236.seq Check: 1590 from: 1 to: 5638
pFN21AB7236 5638 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3448 278
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2981 1937
Size: 1044 4594
Acc65I G'GTAC_C
Cuts at: 2959 2959
Size: 5638
AclI AA'CG_TT
Cuts at: 1563 3765 4138 1563
Size: 2202 373 3063
Fragments arranged by size:
3063 2202 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3701 5085 5353 1632
Size: 2069 1384 268 1917
Fragments arranged by size:
2069 1917 1384 268
AfeI AGC'GCT
Cuts at: 2099 2099
Size: 5638
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5441
AflIII A'CryG_T
Cuts at: 1203 2202 4537 5514 1203
Size: 999 2335 977 1327
Fragments arranged by size:
2335 1327 999 977
AgeI A'CCGG_T
Cuts at: 4469 4469
Size: 5638
AhdI GACnn_n'nnGTC
Cuts at: 4367 4367
Size: 5638
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5638
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5638
AlwNI CAG_nnn'CTG
Cuts at: 2159 2524 2627 3381 4953 2159
Size: 365 103 754 1572 2844
Fragments arranged by size:
2844 1572 754 365 103
ApaI G_GGCC'C
Cuts at: 2298 2298
Size: 5638
ApaLI G'TGCA_C
Cuts at: 3695 4851 3695
Size: 1156 4482
ApoI r'AATT_y
Cuts at: 1493 2947 3173 3325 5434 1493
Size: 1454 226 152 2109 1697
Fragments arranged by size:
2109 1697 1454 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4192 160
Size: 872 3160 1606
Fragments arranged by size:
3160 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
AvaI C'yCGr_G
Cuts at: 1870 1943 2963 1870
Size: 73 1020 4545
Fragments arranged by size:
4545 1020 73
BamHI G'GATC_C
Cuts at: 2968 3335 2968
Size: 367 5271
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2959 4414 618
Size: 325 217 627 1172 1455 1842
Fragments arranged by size:
1842 1455 1172 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2298 2310 2957 1391
Size: 907 12 647 4072
Fragments arranged by size:
4072 907 647 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4667
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2450 2484 3829 3863 1903
Size: 34 513 34 1345 34 3678
Fragments arranged by size:
3678 1345 513 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2252 3530 4740 2252
Size: 1278 1210 3150
Fragments arranged by size:
3150 1278 1210
BclI T'GATC_A
Cuts at: 1548 2421 1548
Size: 873 4765
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4249 136
Size: 107 122 71 631 605 2577 1525
Fragments arranged by size:
2577 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5633 5633
Size: 5638
BlpI GC'TnA_GC
Cuts at: 2309 3055 2309
Size: 746 4892
Bme1580I G_kGCm'C
Cuts at: 1790 2298 3699 4855 1790
Size: 508 1401 1156 2573
Fragments arranged by size:
2573 1401 1156 508
BmgBI CAC'GTC
Cuts at: 1318 2203 2260 2716 1318
Size: 885 57 456 4240
Fragments arranged by size:
4240 885 456 57
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2221 3369 4327
Size: 416 53 83 399 24 804 1148 958
Cuts at: 4327 442
Size: 1753
Fragments arranged by size:
1753 1148 958 804 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2168 2321 2510 2659 4298 1239
Size: 929 153 189 149 1639 2579
Fragments arranged by size:
2579 1639 929 189 153 149
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3763 4628 4926 3763
Size: 865 298 4475
Fragments arranged by size:
4475 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2630 4301 882
Size: 617 1131 1671 2219
Fragments arranged by size:
2219 1671 1131 617
BsaAI yAC'GTr
Cuts at: 493 1204 3429 493
Size: 711 2225 2702
Fragments arranged by size:
2702 2225 711
BsaBI GATnn'nnATC
Cuts at: 5632 5632
Size: 5638
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3445 3827 4462 275
Size: 53 83 186 936 1912 382 635 1451
Fragments arranged by size:
1912 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4071 4469 4743 4890 5301 4071
Size: 398 274 147 411 4408
Fragments arranged by size:
4408 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5413
Fragments arranged by size:
5413 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2410 2846 2867 1186
Size: 1224 436 21 3957
Fragments arranged by size:
3957 1224 436 21
BseYI C'CCAG_C
Cuts at: 2159 2193 2668 4841 5337 2159
Size: 34 475 2173 496 2460
Fragments arranged by size:
2460 2173 496 475 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1998 2227 2627 1163
Size: 573 262 229 400 4174
Fragments arranged by size:
4174 573 400 262 229
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3088 3849 4877 1540
Size: 275 186 1087 761 1028 2301
Fragments arranged by size:
2301 1087 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3699 3784 4855 3699
Size: 85 1071 4482
Fragments arranged by size:
4482 1071 85
BsmI GAATG_Cn'
Cuts at: 3149 3242 3149
Size: 93 5545
BsmBI CGTCTCn'nnnn_
Cuts at: 2369 2369
Size: 5638
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2298 2310 2957 3699 3784 4855 1391
Size: 399 508 12 647 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 647 508 399 85 12
BspEI T'CCGG_A
Cuts at: 5301 5301
Size: 5638
BspHI T'CATG_A
Cuts at: 3527 5257 3527
Size: 1730 3908
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2590 2993 844
Size: 1746 403 3489
Fragments arranged by size:
3489 1746 403
BsrBI CCG'CTC
Cuts at: 1394 1823 3525 1394
Size: 429 1702 3507
Fragments arranged by size:
3507 1702 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4133 4307 65
Size: 1183 2885 174 1396
Fragments arranged by size:
2885 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4282 4469 1700
Size: 110 2472 187 2869
Fragments arranged by size:
2869 2472 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5638
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5638
BssSI C'ACGA_G
Cuts at: 3698 4710 3698
Size: 1012 4626
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5638
BstBI TT'CG_AA
Cuts at: 2502 4442 2502
Size: 1940 3698
BstEII G'GTnAC_C
Cuts at: 1184 2569 1184
Size: 1385 4253
BstYI r'GATC_y
Cuts at: 1972 2968 3335 3720 3737 5189 5275 5287 5633
Size: 996 367 385 17 1452 86 12 346
Cuts at: 5633 1972
Size: 1977
Fragments arranged by size:
1977 1452 996 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2187 2565 2694 2811 513
Size: 552 939 183 378 129 117 3340
Fragments arranged by size:
3340 939 552 378 183 129 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4899
Fragments arranged by size:
4899 683 56
BtsI GCAGTG_nn'
Cuts at: 2076 2215 2434 3154 3948 3968 2076
Size: 139 219 720 794 20 3746
Fragments arranged by size:
3746 794 720 219 139 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5638
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5603
DraI TTT'AAA
Cuts at: 2942 2942
Size: 5638
DraIII CAC_nnn'GTG
Cuts at: 2416 2416
Size: 5638
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4645 809
Size: 507 3329 1802
Fragments arranged by size:
3329 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2112 2930
Size: 54 1425 50 275 18 177 105 818
Cuts at: 2930 3085 3974 5603 8
Size: 155 889 1629 43
Fragments arranged by size:
1629 1425 889 818 275 177 155 105
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3085 1537
Size: 275 1273 4090
Fragments arranged by size:
4090 1273 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2387 2864 3568 1353
Size: 1034 477 704 3423
Fragments arranged by size:
3423 1034 704 477
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4225 4599 4745 387
Size: 1064 138 2636 374 146 1280
Fragments arranged by size:
2636 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2168 2321 2510 2659 3701 4298 5085
Size: 393 536 153 189 149 1042 597 787
Cuts at: 5085 5353 1239
Size: 268 1524
Fragments arranged by size:
1524 1042 787 597 536 393 268 189
153 149
EcoO109I rG'GnC_Cy
Cuts at: 2125 2294 2295 2561 2656 2125
Size: 169 1 266 95 5107
Fragments arranged by size:
5107 266 169 95 1
EcoRI G'AATT_C
Cuts at: 2947 3325 2947
Size: 378 5260
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2396 2396
Size: 5638
FspI TGC'GCA
Cuts at: 4144 4144
Size: 5638
HaeII r_GCGC'y
Cuts at: 1678 2101 4785 1678
Size: 423 2684 2531
Fragments arranged by size:
2684 2531 423
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4279 4311 4353 4385 1373
Size: 32 135 32 2707 32 42 32 2626
Fragments arranged by size:
2707 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2734 2982 3228 669
Size: 988 281 796 248 246 3079
Fragments arranged by size:
3079 988 796 281 248 246
HindIII A'AGCT_T
Cuts at: 748 2588 748
Size: 1840 3798
HpaI GTT'AAC
Cuts at: 3228 3228
Size: 5638
KpnI G_GTAC'C
Cuts at: 2963 2963
Size: 5638
MfeI C'AATT_G
Cuts at: 3237 3237
Size: 5638
MluI A'CGCG_T
Cuts at: 5514 5514
Size: 5638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4752 4936 1305
Size: 3447 184 2007
Fragments arranged by size:
3447 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2114 10
Size: 54 1425 343 282 3534
Fragments arranged by size:
3534 1425 343 282 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2417 3596 3955 4114 518
Size: 648 1251 1179 359 159 2042
Fragments arranged by size:
2042 1251 1179 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2012 2156 2197 2238 2521 2794 2865 3054
Size: 406 144 41 41 283 273 71 189
Cuts at: 3054 3731 4879 5124 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 406 283 273 245 189
144 71 41 41
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4147
Fragments arranged by size:
4147 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5638
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5638
NotI GC'GGCC_GC
Cuts at: 3085 3085
Size: 5638
NspI r_CATG'y
Cuts at: 1132 1229 2996 4541 1132
Size: 97 1767 1545 2229
Fragments arranged by size:
2229 1767 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5638
PciI A'CATG_T
Cuts at: 4537 4537
Size: 5638
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2110 2416 1108
Size: 1002 306 4330
Fragments arranged by size:
4330 1002 306
PfoI T'CCnGG_A
Cuts at: 2652 2675 2652
Size: 23 5615
PmeI GTTT'AAAC
Cuts at: 2942 2942
Size: 5638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3707 3739 5220 5252 3707
Size: 32 1481 32 4093
Fragments arranged by size:
4093 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2125 2656 2125
Size: 531 5107
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5638
PsiI TTA'TAA
Cuts at: 3208 3208
Size: 5638
PspOMI G'GGCC_C
Cuts at: 2294 2294
Size: 5638
PstI C_TGCA'G
Cuts at: 830 2445 2529 2990 830
Size: 1615 84 461 3478
Fragments arranged by size:
3478 1615 461 84
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5638
PvuII CAG'CTG
Cuts at: 2156 2197 2238 2521 2156
Size: 41 41 283 5273
Fragments arranged by size:
5273 283 41 41
SalI G'TCGA_C
Cuts at: 1936 2980 1936
Size: 1044 4594
SbfI CC_TGCA'GG
Cuts at: 2445 2990 2445
Size: 545 5093
ScaI AGT'ACT
Cuts at: 3886 5598 3886
Size: 1712 3926
SexAI A'CCwGG_T
Cuts at: 2103 2103
Size: 5638
SfcI C'TryA_G
Cuts at: 826 1045 2441 2525 2986 4121 4802 4993 826
Size: 219 1396 84 461 1135 681 191 1471
Fragments arranged by size:
1471 1396 1135 681 461 219 191 84
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5638
SmaI CCC'GGG
Cuts at: 1872 2965 1872
Size: 1093 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3742 4643 4905 820
Size: 197 926 1799 901 262 1553
Fragments arranged by size:
1799 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3429 493
Size: 2936 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5638
SphI G_CATG'C
Cuts at: 1132 2996 1132
Size: 1864 3774
SspI AAT'ATT
Cuts at: 5 52 3562 5
Size: 47 3510 2081
Fragments arranged by size:
3510 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5638
StyI C'CwwG_G
Cuts at: 513 1065 2004 2884 3077 513
Size: 552 939 880 193 3074
Fragments arranged by size:
3074 939 880 552 193
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3665 3682 3835 4020 4465 325
Size: 1255 2085 17 153 185 445 1498
Fragments arranged by size:
2085 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3884 5596 96
Size: 274 80 33 51 1443 1907 1712 138
Fragments arranged by size:
1907 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5598 5630 5598
Size: 32 5606
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5638
XbaI T'CTAG_A
Cuts at: 2974 2974
Size: 5638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2125 2485 2125
Size: 360 5278
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5638
XmaI C'CCGG_G
Cuts at: 1870 2963 1870
Size: 1093 4545
XmnI GAAnn'nnTTC
Cuts at: 3767 3767
Size: 5638
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3446 276
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI RsrII SacI SacII
SanDI SapI SfiI SfoI SrfI SwaI