Restriction map for
pFN21AB7232
(Circular) (Six-base) MAPSORT of: pFN21AB7232.seq Check: 2074 from: 1 to: 5893
pFN21AB7232 5893 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2601 2601
Size: 5893
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3703 278
Size: 53 83 186 936 2167 2468
Fragments arranged by size:
2468 2167 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3236 1937
Size: 1299 4594
Acc65I G'GTAC_C
Cuts at: 3214 3214
Size: 5893
AclI AA'CG_TT
Cuts at: 1563 4020 4393 1563
Size: 2457 373 3063
Fragments arranged by size:
3063 2457 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2438 2902 3956 5340 5608 1632
Size: 806 464 1054 1384 268 1917
Fragments arranged by size:
1917 1384 1054 806 464 268
AflII C'TTAA_G
Cuts at: 820 1017 2879 820
Size: 197 1862 3834
Fragments arranged by size:
3834 1862 197
AflIII A'CryG_T
Cuts at: 1203 2731 4792 5769 1203
Size: 1528 2061 977 1327
Fragments arranged by size:
2061 1528 1327 977
AgeI A'CCGG_T
Cuts at: 2295 4724 2295
Size: 2429 3464
AhdI GACnn_n'nnGTC
Cuts at: 2022 4622 2022
Size: 2600 3293
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5893
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2768 1184
Size: 1584 4309
AlwNI CAG_nnn'CTG
Cuts at: 3636 5208 3636
Size: 1572 4321
ApaLI G'TGCA_C
Cuts at: 3950 5106 3950
Size: 1156 4737
ApoI r'AATT_y
Cuts at: 1493 2558 3202 3428 3580 5689 1493
Size: 1065 644 226 152 2109 1697
Fragments arranged by size:
2109 1697 1065 644 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2517 4447 160
Size: 872 1485 1930 1606
Fragments arranged by size:
1930 1606 1485 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5893
AvaI C'yCGr_G
Cuts at: 1870 1943 2956 3218 1870
Size: 73 1013 262 4545
Fragments arranged by size:
4545 1013 262 73
BamHI G'GATC_C
Cuts at: 3223 3590 3223
Size: 367 5526
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2227 3214 4669 618
Size: 325 217 627 440 987 1455 1842
Fragments arranged by size:
1842 1455 987 627 440 325 217
BanII G_rGCy'C
Cuts at: 1391 2279 3212 1391
Size: 888 933 4072
Fragments arranged by size:
4072 933 888
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2018 928
Size: 971 119 4803
Fragments arranged by size:
4803 971 119
BbvCI CC'TCA_GC
Cuts at: 2443 2443
Size: 5893
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2083 2117 4084 4118 1903
Size: 34 146 34 1967 34 3678
Fragments arranged by size:
3678 1967 146 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3785 4995 3785
Size: 1210 4683
BclI T'GATC_A
Cuts at: 1548 3007 1548
Size: 1459 4434
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4504 136
Size: 107 122 71 631 605 337 2495 1525
Fragments arranged by size:
2495 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5888 5888
Size: 5893
BlpI GC'TnA_GC
Cuts at: 2177 3310 2177
Size: 1133 4760
Bme1580I G_kGCm'C
Cuts at: 1790 2232 3954 5110 1790
Size: 442 1722 1156 2573
Fragments arranged by size:
2573 1722 1156 442
BmgBI CAC'GTC
Cuts at: 1318 2926 1318
Size: 1608 4285
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3624 4582 442
Size: 416 53 83 399 24 2207 958 1753
Fragments arranged by size:
2207 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5893
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4553 1239
Size: 3314 2579
Bpu10I CC'TnA_GC
Cuts at: 2153 2443 2153
Size: 290 5603
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4018 4883 5181 4018
Size: 865 298 4730
Fragments arranged by size:
4730 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2194 4556 882
Size: 617 695 2362 2219
Fragments arranged by size:
2362 2219 695 617
BsaAI yAC'GTr
Cuts at: 493 1204 2911 3684 493
Size: 711 1707 773 2702
Fragments arranged by size:
2702 1707 773 711
BsaBI GATnn'nnATC
Cuts at: 5887 5887
Size: 5893
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2111 2220 3700 4082
Size: 53 83 186 936 578 109 1480 382
Cuts at: 4082 4717 275
Size: 635 1451
Fragments arranged by size:
1480 1451 936 635 578 382 186 109
83 53
BsaWI w'CCGG_w
Cuts at: 2080 2295 4326 4724 4998 5145 5556 2080
Size: 215 2031 398 274 147 411 2417
Fragments arranged by size:
2417 2031 411 398 274 215 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5668
Fragments arranged by size:
5668 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2444 2720 1186
Size: 1258 276 4359
Fragments arranged by size:
4359 1258 276
BseYI C'CCAG_C
Cuts at: 2086 2133 2210 5096 5592 2086
Size: 47 77 2886 496 2387
Fragments arranged by size:
2886 2387 496 77 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2640 1163
Size: 573 904 4416
Fragments arranged by size:
4416 904 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3343 4104 5132 1540
Size: 275 186 1342 761 1028 2301
Fragments arranged by size:
2301 1342 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3954 4039 5110 3954
Size: 85 1071 4737
Fragments arranged by size:
4737 1071 85
BsmI GAATG_Cn'
Cuts at: 2608 2993 3404 3497 2608
Size: 385 411 93 5004
Fragments arranged by size:
5004 411 385 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2232 2279 3212 3954 4039 5110 1391
Size: 399 442 47 933 742 85 1071 2174
Fragments arranged by size:
2174 1071 933 742 442 399 85 47
BspEI T'CCGG_A
Cuts at: 2080 5556 2080
Size: 3476 2417
BspHI T'CATG_A
Cuts at: 3782 5512 3782
Size: 1730 4163
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2548 2601 2736 3248 844
Size: 1704 53 135 512 3489
Fragments arranged by size:
3489 1704 512 135 53
BsrBI CCG'CTC
Cuts at: 1394 1823 3780 1394
Size: 429 1957 3507
Fragments arranged by size:
3507 1957 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2789 4388 4562 65
Size: 1183 1541 1599 174 1396
Fragments arranged by size:
1599 1541 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2295 4537 4724 1700
Size: 110 485 2242 187 2869
Fragments arranged by size:
2869 2242 485 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5893
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5893
BssSI C'ACGA_G
Cuts at: 3953 4965 3953
Size: 1012 4881
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5893
BstBI TT'CG_AA
Cuts at: 4697 4697
Size: 5893
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5893
BstXI CCAn_nnnn'nTGG
Cuts at: 2578 2578
Size: 5893
BstYI r'GATC_y
Cuts at: 1972 3223 3590 3975 3992 5444 5530 5542 5888
Size: 1251 367 385 17 1452 86 12 346
Cuts at: 5888 1972
Size: 1977
Fragments arranged by size:
1977 1452 1251 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3075 3075
Size: 5893
BtgI C'CryG_G
Cuts at: 513 1065 2004 2223 2424 2674 2980 513
Size: 552 939 219 201 250 306 3426
Fragments arranged by size:
3426 939 552 306 250 219 201
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5154
Fragments arranged by size:
5154 683 56
BtsI GCAGTG_nn'
Cuts at: 3409 4203 4223 3409
Size: 794 20 5079
Fragments arranged by size:
5079 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5893
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5858
DraI TTT'AAA
Cuts at: 3197 3197
Size: 5893
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4900 809
Size: 507 3584 1802
Fragments arranged by size:
3584 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2806 3340
Size: 54 1425 50 275 18 180 796 534
Cuts at: 3340 4229 5858 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 796 534 275 180 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3340 1537
Size: 275 1528 4090
Fragments arranged by size:
4090 1528 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3823 1353
Size: 2470 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4480 4854 5000 387
Size: 1064 138 2891 374 146 1280
Fragments arranged by size:
2891 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2438 2902 3956 4553 5340 5608 1239
Size: 393 806 464 1054 597 787 268 1524
Fragments arranged by size:
1524 1054 806 787 597 464 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2016 2050 2149 2016
Size: 34 99 5760
Fragments arranged by size:
5760 99 34
EcoRI G'AATT_C
Cuts at: 3202 3580 3202
Size: 378 5515
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2766 2766
Size: 5893
FspI TGC'GCA
Cuts at: 2333 4399 2333
Size: 2066 3827
HaeII r_GCGC'y
Cuts at: 1678 5040 1678
Size: 3362 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2008 2040 2935 2967 4534
Size: 32 135 32 436 32 895 32 1567
Cuts at: 4534 4566 4608 4640 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1567 895 436 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3052 3061 3237 3483 669
Size: 988 281 1114 9 176 246 3079
Fragments arranged by size:
3079 1114 988 281 246 176 9
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5893
HpaI GTT'AAC
Cuts at: 3061 3483 3061
Size: 422 5471
KpnI G_GTAC'C
Cuts at: 3218 3218
Size: 5893
MfeI C'AATT_G
Cuts at: 3492 3492
Size: 5893
MluI A'CGCG_T
Cuts at: 5769 5769
Size: 5893
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2280 5007 5191 1305
Size: 975 2727 184 2007
Fragments arranged by size:
2727 2007 975 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2808 10
Size: 54 1425 343 976 3095
Fragments arranged by size:
3095 1425 976 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2760 3170 3851 4210 4369 518
Size: 648 1594 410 681 359 159 2042
Fragments arranged by size:
2042 1594 681 648 410 359 159
MspA1I CmG'CkG
Cuts at: 1606 2022 2639 2772 2869 2889 3309 3986 5134
Size: 416 617 133 97 20 420 677 1148
Cuts at: 5134 5379 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 617 420 416 245 133
97 20
NcoI C'CATG_G
Cuts at: 513 1065 2004 2674 513
Size: 552 939 670 3732
Fragments arranged by size:
3732 939 670 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5893
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5893
NotI GC'GGCC_GC
Cuts at: 3340 3340
Size: 5893
NspI r_CATG'y
Cuts at: 1132 1229 2185 2242 2735 3084 3251 4796 1132
Size: 97 956 57 493 349 167 1545 2229
Fragments arranged by size:
2229 1545 956 493 349 167 97 57
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5893
PciI A'CATG_T
Cuts at: 2731 4792 2731
Size: 2061 3832
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2299 1108
Size: 1191 4702
PmeI GTTT'AAAC
Cuts at: 3197 3197
Size: 5893
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3962 3994 5475 5507 3962
Size: 32 1481 32 4348
Fragments arranged by size:
4348 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2016 2050 2149 2016
Size: 34 99 5760
Fragments arranged by size:
5760 99 34
PshAI GACnn'nnGTC
Cuts at: 1613 3047 1613
Size: 1434 4459
PsiI TTA'TAA
Cuts at: 3463 3463
Size: 5893
PstI C_TGCA'G
Cuts at: 830 3245 830
Size: 2415 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5893
PvuII CAG'CTG
Cuts at: 2772 2889 2772
Size: 117 5776
SalI G'TCGA_C
Cuts at: 1936 3235 1936
Size: 1299 4594
SanDI GG'GwC_CC
Cuts at: 2016 2016
Size: 5893
SbfI CC_TGCA'GG
Cuts at: 3245 3245
Size: 5893
ScaI AGT'ACT
Cuts at: 4141 5853 4141
Size: 1712 4181
SexAI A'CCwGG_T
Cuts at: 2585 2585
Size: 5893
SfcI C'TryA_G
Cuts at: 826 1045 3241 4376 5057 5248 826
Size: 219 2196 1135 681 191 1471
Fragments arranged by size:
2196 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5893
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5893
SmaI CCC'GGG
Cuts at: 1872 2958 3220 1872
Size: 1086 262 4545
Fragments arranged by size:
4545 1086 262
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2879 3997 4898 5160 820
Size: 197 926 936 1118 901 262 1553
Fragments arranged by size:
1553 1118 936 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3684 493
Size: 3191 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5893
SphI G_CATG'C
Cuts at: 1132 3084 3251 1132
Size: 1952 167 3774
Fragments arranged by size:
3774 1952 167
SspI AAT'ATT
Cuts at: 5 52 2521 3817 5
Size: 47 2469 1296 2081
Fragments arranged by size:
2469 2081 1296 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5893
StyI C'CwwG_G
Cuts at: 513 1065 2004 2674 3332 513
Size: 552 939 670 658 3074
Fragments arranged by size:
3074 939 670 658 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3174 3920 3937 4090 4275 4720 325
Size: 1255 1594 746 17 153 185 445 1498
Fragments arranged by size:
1594 1498 1255 746 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4139 5851 96
Size: 274 80 33 51 1443 2162 1712 138
Fragments arranged by size:
2162 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2670 2702 5853 5885 2670
Size: 32 3151 32 2678
Fragments arranged by size:
3151 2678 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2147 1316
Size: 831 5062
XbaI T'CTAG_A
Cuts at: 3229 3229
Size: 5893
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5893
XmaI C'CCGG_G
Cuts at: 1870 2956 3218 1870
Size: 1086 262 4545
Fragments arranged by size:
4545 1086 262
XmnI GAAnn'nnTTC
Cuts at: 4022 4022
Size: 5893
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3701 276
Size: 53 83 186 936 2167 2468
Fragments arranged by size:
2468 2167 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SapI SfiI SfoI SrfI SwaI XcmI