Restriction map for
pFN21AB7231
(Circular) (Six-base) MAPSORT of: pFN21AB7231.seq Check: 6266 from: 1 to: 6007
pFN21AB7231 6007 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2136 2136
Size: 6007
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3817 278
Size: 53 83 186 936 2281 2468
Fragments arranged by size:
2468 2281 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3350 1937
Size: 1413 4594
Acc65I G'GTAC_C
Cuts at: 2447 3328 2447
Size: 881 5126
AclI AA'CG_TT
Cuts at: 1563 3164 4134 4507 1563
Size: 1601 970 373 3063
Fragments arranged by size:
3063 1601 970 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2242 4070 5454 5722 1632
Size: 610 1828 1384 268 1917
Fragments arranged by size:
1917 1828 1384 610 268
AfeI AGC'GCT
Cuts at: 2622 2622
Size: 6007
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5810
AflIII A'CryG_T
Cuts at: 1203 2181 4906 5883 1203
Size: 978 2725 977 1327
Fragments arranged by size:
2725 1327 978 977
AgeI A'CCGG_T
Cuts at: 4838 4838
Size: 6007
AhdI GACnn_n'nnGTC
Cuts at: 3256 4736 3256
Size: 1480 4527
AleI CACnn'nnGTG
Cuts at: 1166 2540 1166
Size: 1374 4633
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2165 1184
Size: 981 5026
AlwNI CAG_nnn'CTG
Cuts at: 3750 5322 3750
Size: 1572 4435
ApaI G_GGCC'C
Cuts at: 2244 2244
Size: 6007
ApaLI G'TGCA_C
Cuts at: 2150 4064 5220 2150
Size: 1914 1156 2937
Fragments arranged by size:
2937 1914 1156
ApoI r'AATT_y
Cuts at: 1493 3221 3316 3542 3694 5803 1493
Size: 1728 95 226 152 2109 1697
Fragments arranged by size:
2109 1728 1697 226 152 95
AseI AT'TA_AT
Cuts at: 160 1032 4561 160
Size: 872 3529 1606
Fragments arranged by size:
3529 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6007
AvaI C'yCGr_G
Cuts at: 1870 1943 2236 3332 1870
Size: 73 293 1096 4545
Fragments arranged by size:
4545 1096 293 73
BamHI G'GATC_C
Cuts at: 3337 3704 3337
Size: 367 5640
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2054 2105 2216 2447 3328
Size: 325 217 627 267 51 111 231 881
Cuts at: 3328 4783 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 881 627 325 267 231 217
111 51
BanII G_rGCy'C
Cuts at: 1391 2244 2550 2871 3326 1391
Size: 853 306 321 455 4072
Fragments arranged by size:
4072 853 455 321 306
BbeI G_GCGC'C
Cuts at: 2058 2058
Size: 6007
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2248 928
Size: 971 349 4687
Fragments arranged by size:
4687 971 349
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4198 4232 1903
Size: 34 2261 34 3678
Fragments arranged by size:
3678 2261 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3899 5109 3899
Size: 1210 4797
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6007
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2248 4618 136
Size: 107 122 71 631 605 576 2370 1525
Fragments arranged by size:
2370 1525 631 605 576 122 107 71
BglII A'GATC_T
Cuts at: 2466 6002 2466
Size: 3536 2471
BlpI GC'TnA_GC
Cuts at: 2865 3424 2865
Size: 559 5448
Bme1580I G_kGCm'C
Cuts at: 1790 2154 2219 2244 4068 5224 1790
Size: 364 65 25 1824 1156 2573
Fragments arranged by size:
2573 1824 1156 364 65 25
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6007
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2314 2442 3117
Size: 416 53 83 399 24 897 128 675
Cuts at: 3117 3738 4696 442
Size: 621 958 1753
Fragments arranged by size:
1753 958 897 675 621 416 399 128
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6007
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2092 2092
Size: 6007
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2228 4667 1239
Size: 917 72 2439 2579
Fragments arranged by size:
2579 2439 917 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3119 4132 4997 5295 3119
Size: 1013 865 298 3831
Fragments arranged by size:
3831 1013 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2591 3148 4670 882
Size: 617 1092 557 1522 2219
Fragments arranged by size:
2219 1522 1092 617 557
BsaAI yAC'GTr
Cuts at: 493 1204 2182 2718 3798 493
Size: 711 978 536 1080 2702
Fragments arranged by size:
2702 1080 978 711 536
BsaBI GATnn'nnATC
Cuts at: 6001 6001
Size: 6007
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2055 3814 4196 4831
Size: 53 83 186 936 522 1759 382 635
Cuts at: 4831 275
Size: 1451
Fragments arranged by size:
1759 1451 936 635 522 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4440 4838 5112 5259 5670 4440
Size: 398 274 147 411 4777
Fragments arranged by size:
4777 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3288 3291 3318 3321 1178
Size: 30 165 30 1885 3 27 3 3864
Fragments arranged by size:
3864 1885 165 30 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2489 3312 3315 1186
Size: 1303 823 3 3878
Fragments arranged by size:
3878 1303 823 3
BseYI C'CCAG_C
Cuts at: 2874 5210 5706 2874
Size: 2336 496 3175
Fragments arranged by size:
3175 2336 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5434
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2929 3291 3457 4218 5246 1540
Size: 275 186 928 362 166 761 1028 2301
Fragments arranged by size:
2301 1028 928 761 362 275 186 166
BsiHKAI G_wGCw'C
Cuts at: 2022 2154 2199 2871 4068 4153 5224 2022
Size: 132 45 672 1197 85 1071 2805
Fragments arranged by size:
2805 1197 1071 672 132 85 45
BsmI GAATG_Cn'
Cuts at: 2749 3518 3611 2749
Size: 769 93 5145
Fragments arranged by size:
5145 769 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2154 2199 2219 2244 2550 2871
Size: 399 232 132 45 20 25 306 321
Cuts at: 2871 3326 4068 4153 5224 1391
Size: 455 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 455 399 321 306 232
132 85 45 25 20
BspEI T'CCGG_A
Cuts at: 5670 5670
Size: 6007
BspHI T'CATG_A
Cuts at: 3896 5626 3896
Size: 1730 4277
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2136 2166 2242 2760 3362 844
Size: 1292 30 76 518 602 3489
Fragments arranged by size:
3489 1292 602 518 76 30
BsrBI CCG'CTC
Cuts at: 1394 1823 3894 1394
Size: 429 2071 3507
Fragments arranged by size:
3507 2071 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4502 4676 65
Size: 1183 3254 174 1396
Fragments arranged by size:
3254 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4651 4838 1700
Size: 110 2841 187 2869
Fragments arranged by size:
2869 2841 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6007
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6007
BssSI C'ACGA_G
Cuts at: 2159 2198 4067 5079 2159
Size: 39 1869 1012 3087
Fragments arranged by size:
3087 1869 1012 39
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6007
BstBI TT'CG_AA
Cuts at: 4811 4811
Size: 6007
BstEII G'GTnAC_C
Cuts at: 1184 2672 2710 1184
Size: 1488 38 4481
Fragments arranged by size:
4481 1488 38
BstXI CCAn_nnnn'nTGG
Cuts at: 2773 2773
Size: 6007
BstYI r'GATC_y
Cuts at: 1972 2203 2466 3337 3704 4089 4106 5558 5644
Size: 231 263 871 367 385 17 1452 86
Cuts at: 5644 5656 6002 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 871 385 367 346 263 231
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2113 2220 2538 3285 513
Size: 552 1048 107 318 747 3235
Fragments arranged by size:
3235 1048 747 552 318 107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5268
Fragments arranged by size:
5268 683 56
BtsI GCAGTG_nn'
Cuts at: 2042 3523 4317 4337 2042
Size: 1481 794 20 3712
Fragments arranged by size:
3712 1481 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6007
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5972
DraI TTT'AAA
Cuts at: 3311 3311
Size: 6007
DraIII CAC_nnn'GTG
Cuts at: 2689 2718 2689
Size: 29 5978
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5014 809
Size: 507 3698 1802
Fragments arranged by size:
3698 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2763 2926 3288
Size: 54 1425 50 275 18 933 163 362
Cuts at: 3288 3454 4343 5972 8
Size: 166 889 1629 43
Fragments arranged by size:
1629 1425 933 889 362 275 166 163
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2926 3288 3454 1537
Size: 275 1114 362 166 4090
Fragments arranged by size:
4090 1114 362 275 166
EarI CTCTTCn'nnn_
Cuts at: 1353 3937 1353
Size: 2584 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4594 4968 5114 387
Size: 1064 138 3005 374 146 1280
Fragments arranged by size:
3005 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2869 2869
Size: 6007
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2228 2242 4070 4667 5454 5722
Size: 393 524 72 14 1828 597 787 268
Cuts at: 5722 1239
Size: 1524
Fragments arranged by size:
1828 1524 787 597 524 393 268 72
14
EcoO109I rG'GnC_Cy
Cuts at: 2240 2241 2643 2240
Size: 1 402 5604
Fragments arranged by size:
5604 402 1
EcoRI G'AATT_C
Cuts at: 3221 3316 3694 3221
Size: 95 378 5534
Fragments arranged by size:
5534 378 95
FspI TGC'GCA
Cuts at: 4513 4513
Size: 6007
HaeII r_GCGC'y
Cuts at: 1678 2058 2624 5154 1678
Size: 380 566 2530 2531
Fragments arranged by size:
2531 2530 566 380
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3242 3274 4648 4680 4722
Size: 32 135 32 1670 32 1374 32 42
Cuts at: 4722 4754 1373
Size: 32 2626
Fragments arranged by size:
2626 1670 1374 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3351 3597 669
Size: 988 281 1413 246 3079
Fragments arranged by size:
3079 1413 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6007
HpaI GTT'AAC
Cuts at: 3597 3597
Size: 6007
KasI G'GCGC_C
Cuts at: 2054 2054
Size: 6007
KpnI G_GTAC'C
Cuts at: 2451 3332 2451
Size: 881 5126
MfeI C'AATT_G
Cuts at: 3606 3606
Size: 6007
MluI A'CGCG_T
Cuts at: 5883 5883
Size: 6007
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5121 5305 1305
Size: 3816 184 2007
Fragments arranged by size:
3816 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2765 10
Size: 54 1425 343 933 3252
Fragments arranged by size:
3252 1425 933 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2147 2540 2771 2969 3965 4324 4483
Size: 648 981 393 231 198 996 359 159
Cuts at: 4483 518
Size: 2042
Fragments arranged by size:
2042 996 981 648 393 359 231 198
159
MspA1I CmG'CkG
Cuts at: 1606 2102 2134 2169 2407 2740 3423 4100 5248
Size: 496 32 35 238 333 683 677 1148
Cuts at: 5248 5493 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 683 677 496 333 245 238
35 32
NarI GG'CG_CC
Cuts at: 2055 2055
Size: 6007
NcoI C'CATG_G
Cuts at: 513 1065 2220 513
Size: 552 1155 4300
Fragments arranged by size:
4300 1155 552
NdeI CA'TA_TG
Cuts at: 387 2633 387
Size: 2246 3761
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6007
NotI GC'GGCC_GC
Cuts at: 3454 3454
Size: 6007
NspI r_CATG'y
Cuts at: 1132 1229 2090 2968 3365 4910 1132
Size: 97 861 878 397 1545 2229
Fragments arranged by size:
2229 1545 878 861 397 97
PasI CC'CwG_GG
Cuts at: 1348 2419 3078 1348
Size: 1071 659 4277
Fragments arranged by size:
4277 1071 659
PciI A'CATG_T
Cuts at: 4906 4906
Size: 6007
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2064 2419 1108
Size: 956 355 4696
Fragments arranged by size:
4696 956 355
PfoI T'CCnGG_A
Cuts at: 2639 3126 2639
Size: 487 5520
PmeI GTTT'AAAC
Cuts at: 3311 3311
Size: 6007
PmlI CAC'GTG
Cuts at: 2718 2718
Size: 6007
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4076 4108 5589 5621 4076
Size: 32 1481 32 4462
Fragments arranged by size:
4462 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2643 2643
Size: 6007
PshAI GACnn'nnGTC
Cuts at: 1613 2919 1613
Size: 1306 4701
PsiI TTA'TAA
Cuts at: 2495 3577 2495
Size: 1082 4925
PspOMI G'GGCC_C
Cuts at: 2240 2240
Size: 6007
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3005 3037 3005
Size: 32 5975
PstI C_TGCA'G
Cuts at: 830 2133 2397 3359 830
Size: 1303 264 962 3478
Fragments arranged by size:
3478 1303 962 264
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6007
PvuII CAG'CTG
Cuts at: 2134 2407 2740 2134
Size: 273 333 5401
Fragments arranged by size:
5401 333 273
SacI G_AGCT'C
Cuts at: 2871 2871
Size: 6007
SalI G'TCGA_C
Cuts at: 1936 3349 1936
Size: 1413 4594
SbfI CC_TGCA'GG
Cuts at: 2397 3359 2397
Size: 962 5045
ScaI AGT'ACT
Cuts at: 4255 5967 4255
Size: 1712 4295
SexAI A'CCwGG_T
Cuts at: 2685 2685
Size: 6007
SfcI C'TryA_G
Cuts at: 826 1045 2129 2393 3355 4490 5171 5362 826
Size: 219 1084 264 962 1135 681 191 1471
Fragments arranged by size:
1471 1135 1084 962 681 264 219 191
SfoI GGC'GCC
Cuts at: 2056 2056
Size: 6007
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6007
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6007
SmaI CCC'GGG
Cuts at: 1872 3334 1872
Size: 1462 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3134 4111 5012 5274 820
Size: 197 926 1191 977 901 262 1553
Fragments arranged by size:
1553 1191 977 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3798 493
Size: 3305 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6007
SphI G_CATG'C
Cuts at: 1132 2968 3365 1132
Size: 1836 397 3774
Fragments arranged by size:
3774 1836 397
SspI AAT'ATT
Cuts at: 5 52 3931 5
Size: 47 3879 2081
Fragments arranged by size:
3879 2081 47
StuI AGG'CCT
Cuts at: 1517 3179 1517
Size: 1662 4345
StyI C'CwwG_G
Cuts at: 513 1065 2220 2310 2523 3446 513
Size: 552 1155 90 213 923 3074
Fragments arranged by size:
3074 1155 923 552 213 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2411 2677 2888 4034 4051 4204 4389
Size: 1255 831 266 211 1146 17 153 185
Cuts at: 4389 4834 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1146 831 445 266 211 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4253 5965 96
Size: 274 80 33 51 1443 2276 1712 138
Fragments arranged by size:
2276 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5967 5999 5967
Size: 32 5975
Tth111I GACn'n_nGTC
Cuts at: 1316 2344 2360 2434 1316
Size: 1028 16 74 4889
Fragments arranged by size:
4889 1028 74 16
XbaI T'CTAG_A
Cuts at: 3343 3343
Size: 6007
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2420 2977 2420
Size: 557 5450
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6007
XmaI C'CCGG_G
Cuts at: 1870 3332 1870
Size: 1462 4545
XmnI GAAnn'nnTTC
Cuts at: 4136 4136
Size: 6007
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3815 276
Size: 53 83 186 936 2281 2468
Fragments arranged by size:
2468 2281 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII SacI SalI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PspXI RsrII SacII
SanDI SapI SfiI SrfI SwaI